This study attempted to clarify the carrying-over effect of different nitrogen treatments applied to the main crop on the crop population growth and yield formation of ratoon rice under mechanized cultivation in South...This study attempted to clarify the carrying-over effect of different nitrogen treatments applied to the main crop on the crop population growth and yield formation of ratoon rice under mechanized cultivation in Southeast China.Based on the constant total nitrogen application amounts(225.00 kg ha;)in the main crop,an experiment with different ratios of basal and topdressing nitrogen fertilizer(the ratio of basal fertilizer:primary tillering fertilizer:secondary tillering fertilizer:booting fertilizer at 3:1:2:4(N1),3:2:1:4(N2),3:3:0:4(N3),and 4:3:0:3(N4),respectively,and a control without nitrogen treatment(N0))was set up across two consecutive years in field using hybrid rice variety Yongyou 1540 as the test materials.The results showed that the total tiller number and effective tillering percentage increased in the main crop under the N1 treatment,more nitrogen fertilizer applied in late growth stage of the main crop,and its effective tillering percentage of the main crop was the highest at up to 70.18%,which was 9.15%higher than that of conventional fertilization treatment(N4),more nitrogen fertilizer applied in early growth stage of the main crop.The same tendency was observed in leaf area index(LAI)value of the main crop and its subsequent ratoon rice,which were 16.52 and 29.87%higher,respectively,in the N1 treatment than that in the N4 treatment at the full heading stage.The same was true in the case of the transport rates of stem and sheath dry mater and the canopy light interception rates in both the main and its ratoon crops.The transport rate of stem and sheath in main crop rice under N1 treatment increased by 50.57%compared with N4 treatment.The canopy light interception rate of N1 treatment increased by 5.07%compared with N4 treatment at the full heading stage of the ratoon crop.Therefore,the total actual yield was the highest in the main and its ratoon crops under N1 treatment,averaging 17351.23 kg ha;in two-year trials,which was 23.00%higher than that in the conventional fertilization treatment(N4).The results showed that appropriate nitrogen treatment was able to produce a good crop stand in the main crop,which was essential for producing a good ratoon crop population and high yield especially under mechanized cultivation with low stubble height of the main crop.The study suggested that shifting the proper nitrogen application amounts to the late growth stage of the main crop,such as N1 treatment,not only had a higher productive effect on ensuring the yield of the main crop,but also had a positive effect on the axillary bud sprouts from the stubbles for ratoon rice,resulting in an increased percentage of productive panicles and achieving the goal of one planting with two good harvests under the conditions of our study.展开更多
To identify the function of differential expression proteins in different leaves of rice seedlings extracted from 2- to 5-leaf stages, the leaf proteins at the seedling stage of hybrid rice Shanyou 63 were studied by ...To identify the function of differential expression proteins in different leaves of rice seedlings extracted from 2- to 5-leaf stages, the leaf proteins at the seedling stage of hybrid rice Shanyou 63 were studied by using the approach of plant proteomics, and those proteins were separated with two-dimensional electrophoresis （2-DE） and then analyzed with an imagemaster 2D Elite 5.0. The results showed that the 41 protein spots were detected differential expression, of which 17 new protein spots appeared after the 3-leaf stage, including 9 special protein spots, which were only detected at the 3-leaf stage. Thirteen protein spots increased first and then decreased in expression abundance gradually and finally even disappeared. For the other 11 protein spots, 3 protein spots decreased, but 6 protein spots were opposite in expression abundance, however, 2 protein spots expressed in an irregular pattern after the 2-leaf stage. Of the 41 differential leaf proteins, 15 protein spots were identified by ESI-Q MS/MS and categorized into 4 groups of functions. The results indicated that proteins were the carriers of the functions in cells, but were significantly influenced by the changes in cell function or intercellular environment; hence, the reason that caused the proteomic changes as mentioned earlier might be related to the occurrence of tillers at the rice seedling stage after the 3-leaf stage.展开更多
基金funded by the National Key Research and Development Program of China(2016YFD0300508,2017YFD0301602 and 2018YFD0301105)the FujianTaiwan Joint Innovative Centre for Germplasm Resources and Cultivation of Crop,China(Fujian 2011 Program+1 种基金2015-75)the Science and Technology Development Fund of Fujian Agriculture and Forestry University,China(KF2015043)。
文摘This study attempted to clarify the carrying-over effect of different nitrogen treatments applied to the main crop on the crop population growth and yield formation of ratoon rice under mechanized cultivation in Southeast China.Based on the constant total nitrogen application amounts(225.00 kg ha;)in the main crop,an experiment with different ratios of basal and topdressing nitrogen fertilizer(the ratio of basal fertilizer:primary tillering fertilizer:secondary tillering fertilizer:booting fertilizer at 3:1:2:4(N1),3:2:1:4(N2),3:3:0:4(N3),and 4:3:0:3(N4),respectively,and a control without nitrogen treatment(N0))was set up across two consecutive years in field using hybrid rice variety Yongyou 1540 as the test materials.The results showed that the total tiller number and effective tillering percentage increased in the main crop under the N1 treatment,more nitrogen fertilizer applied in late growth stage of the main crop,and its effective tillering percentage of the main crop was the highest at up to 70.18%,which was 9.15%higher than that of conventional fertilization treatment(N4),more nitrogen fertilizer applied in early growth stage of the main crop.The same tendency was observed in leaf area index(LAI)value of the main crop and its subsequent ratoon rice,which were 16.52 and 29.87%higher,respectively,in the N1 treatment than that in the N4 treatment at the full heading stage.The same was true in the case of the transport rates of stem and sheath dry mater and the canopy light interception rates in both the main and its ratoon crops.The transport rate of stem and sheath in main crop rice under N1 treatment increased by 50.57%compared with N4 treatment.The canopy light interception rate of N1 treatment increased by 5.07%compared with N4 treatment at the full heading stage of the ratoon crop.Therefore,the total actual yield was the highest in the main and its ratoon crops under N1 treatment,averaging 17351.23 kg ha;in two-year trials,which was 23.00%higher than that in the conventional fertilization treatment(N4).The results showed that appropriate nitrogen treatment was able to produce a good crop stand in the main crop,which was essential for producing a good ratoon crop population and high yield especially under mechanized cultivation with low stubble height of the main crop.The study suggested that shifting the proper nitrogen application amounts to the late growth stage of the main crop,such as N1 treatment,not only had a higher productive effect on ensuring the yield of the main crop,but also had a positive effect on the axillary bud sprouts from the stubbles for ratoon rice,resulting in an increased percentage of productive panicles and achieving the goal of one planting with two good harvests under the conditions of our study.
基金the National Natural Science Foundation of China (30600385)the National Key Technology R&D Program of China (2004NZ0104)
文摘To identify the function of differential expression proteins in different leaves of rice seedlings extracted from 2- to 5-leaf stages, the leaf proteins at the seedling stage of hybrid rice Shanyou 63 were studied by using the approach of plant proteomics, and those proteins were separated with two-dimensional electrophoresis （2-DE） and then analyzed with an imagemaster 2D Elite 5.0. The results showed that the 41 protein spots were detected differential expression, of which 17 new protein spots appeared after the 3-leaf stage, including 9 special protein spots, which were only detected at the 3-leaf stage. Thirteen protein spots increased first and then decreased in expression abundance gradually and finally even disappeared. For the other 11 protein spots, 3 protein spots decreased, but 6 protein spots were opposite in expression abundance, however, 2 protein spots expressed in an irregular pattern after the 2-leaf stage. Of the 41 differential leaf proteins, 15 protein spots were identified by ESI-Q MS/MS and categorized into 4 groups of functions. The results indicated that proteins were the carriers of the functions in cells, but were significantly influenced by the changes in cell function or intercellular environment; hence, the reason that caused the proteomic changes as mentioned earlier might be related to the occurrence of tillers at the rice seedling stage after the 3-leaf stage.
