Degradation under challenging conditions such as rain, haze, and low light not only diminishes content visibility, but also results in additional degradation side effects, including detail occlusion and color distorti...Degradation under challenging conditions such as rain, haze, and low light not only diminishes content visibility, but also results in additional degradation side effects, including detail occlusion and color distortion. However, current technologies have barely explored the correlation between perturbation removal and background restoration, consequently struggling to generate high-naturalness content in challenging scenarios. In this paper, we rethink the image enhancement task from the perspective of joint optimization: Perturbation removal and texture reconstruction. To this end, we advise an efficient yet effective image enhancement model, termed the perturbation-guided texture reconstruction network(PerTeRNet). It contains two subnetworks designed for the perturbation elimination and texture reconstruction tasks, respectively. To facilitate texture recovery,we develop a novel perturbation-guided texture enhancement module(PerTEM) to connect these two tasks, where informative background features are extracted from the input with the guidance of predicted perturbation priors. To alleviate the learning burden and computational cost, we suggest performing perturbation removal in a sub-space and exploiting super-resolution to infer high-frequency background details. Our PerTeRNet has demonstrated significant superiority over typical methods in both quantitative and qualitative measures, as evidenced by extensive experimental results on popular image enhancement and joint detection tasks. The source code is available at https://github.com/kuijiang94/PerTeRNet.展开更多
Selective clearance of organelles,including endoplasmic reticulum(ER)and mitochondria,by autophagy plays an important role in cell health.Here,we describe a developmentally programmed selective ER clearance by autopha...Selective clearance of organelles,including endoplasmic reticulum(ER)and mitochondria,by autophagy plays an important role in cell health.Here,we describe a developmentally programmed selective ER clearance by autophagy.We show that Parkinson's disease-associated PINK1,as well as Atl,Rtnl1,and Trp1 receptors,regulate ER clearance by autophagy.展开更多
Background:Although more and more attention has been paid to the psychological consequences of the lockdown policy amongst pregnant women,the underlying mechanism linking the lockdown policy to maternal depression has...Background:Although more and more attention has been paid to the psychological consequences of the lockdown policy amongst pregnant women,the underlying mechanism linking the lockdown policy to maternal depression has not been studied in the context of China.This study aimed to explore the association between the lockdown policy and maternal depressive symptoms,and whether such association was mediated by internet use and/or family support.Methods:This cross-sectional study used multi-stage sampling techniques in central and western China.Data were collected from 1266 pregnant women using a structtured questionnaire that measured internet use,family support,and depressive symptoms.The Patient Health Questionnaire-9(PHQ-9)was used to measure depressive symptoms.Internet use was measured by length of usage and varierity of purpose for internet use.Family support was measureed by spousal support and parental support.The structural equation modelling was employed to conduct mediation analysis to test the specificity of the hypothetical paths.Results:Overall,527 respondents(41.63%)presented depressive symptoms.The lockdown policy was negatively associated with depressive symptoms in pregnant women(β=−0.925,95%CI=−1.510,−0.360).The impact of the lockdown policy on depressive symptoms was partially mediated by internet use(β=1.589,95%CI=0.730,2.807)and family support(β=−0.162,95%CI=−0.341,−0.017),accounting for 42.67%of the total effect.Conclusions:The lockdown policy was generally associated with fewer depressive symptoms in pregnant women.The lockdown policy increased maternal depressive symptoms through increased internet use,but decreased maternal depressive symptoms through enhanced family support.The findings suggest that the psychological consequence of the lockdown policy may vary across different populations,and warrant the need to take into consideration the features of subgroups.展开更多
Osteosarcoma(OS)is one of the most common malignant tumors in children and young adults.As chemotherapy and other therapies are limited by low therapeutic efficiency,severe side effects and single therapeutic function...Osteosarcoma(OS)is one of the most common malignant tumors in children and young adults.As chemotherapy and other therapies are limited by low therapeutic efficiency,severe side effects and single therapeutic function,it is of high value to develop innovative therapies for precise and efficient treatment of OS.Herein,natural photo-synthetic microalgae(C.vulgaris,CV)were utilized as carriers for the chemotherapeutic agent doxorubicin(DOX)to create a multifunctional therapeutic platform(CV@DOX)for the photo-modulation of the tumor microenvi-ronment(TME)and synergistic chemo-photodynamic therapy of osteosarcoma.CV@DOX exhibited rapid drug release behavior in the acidic TME,improving the efficiency of chemotherapy against tumors and reducing side effects on other normal tissues.Under 650 nm laser irradiation,CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species(ROS),thereby enhancing photodynamic therapy(PDT).CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis,inhibiting tumor proliferation and angiogenesis.Moreover,chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after administration.Finally,CV@DOX did not cause significant hematological and tissue toxicity,and prevented DOX-induced cardiotoxicity,showing good in vivo biocompatibility.Overall,this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosar-coma treatment.展开更多
Meiosis produces the haploid gametes required by all sexually reproducing organisms,occurring in specific temperature ranges in different organisms.However,how meiotic thermotolerance is regulated remains largely unkn...Meiosis produces the haploid gametes required by all sexually reproducing organisms,occurring in specific temperature ranges in different organisms.However,how meiotic thermotolerance is regulated remains largely unknown.Using the model organism Caenorhabditis elegans,here,we identified the synaptonemal complex(SC)protein SYP-5 as a critical regulator of meiotic thermotolerance.syp-5-null mutants maintained a high percentage of viable progeny at 20℃ but produced significantly fewer viable progeny at 25℃,a permissive temperature in wild-type worms.Cytological analysis of meiotic events in the mutants revealed that while SC assembly and disassembly,as well as DNA double-strand break repair kinetics,were not affected by the elevated temperature,crossover designation,and bivalent formation were significantly affected.More severe homolog segregation errors were also observed at elevated temperature.A temperature switching assay revealed that late meiotic prophase events were not temperature-sensitive and that meiotic defects during pachytene stage were responsible for the reduced viability of syp-5 mutants at the elevated temperature.Moreover,SC polycomplex formation and hexanediol sensitivity analysis suggested that SYP-5 was required for the normal properties of the SC,and charge-interacting elements in SC components were involved in regulating meiotic thermotolerance.Together,these findings provide a novel molecular mechanism for meiotic thermotolerance regulation.展开更多
Androgenetic alopecia(AGA)is an androgen-mediated alopecia affected by both genes and hormones.Medication is a relatively common treatment.As a new drug delivery method,microneedles(MNs)can effectively break through t...Androgenetic alopecia(AGA)is an androgen-mediated alopecia affected by both genes and hormones.Medication is a relatively common treatment.As a new drug delivery method,microneedles(MNs)can effectively break through the stratum corneum barrier,deliver drugs more efficiently,and achieve better therapeutic effects.In this study,we develop a composite double-layer MN through multi-step casting fabrication using a polydimethylsiloxane mold.The needle tip was fabricated by mixed solution of chitosan and polyvinylpyrrolidone which was loaded with Polygonum multiflorum extract,and the base layer was prepared by mixed solution of polyvinyl alcohol and polyvinylpyrrolidone.In vitro mechanical tests showed that the maximum load of a single tip of the drug-loaded MN was about 3.5 N,which met the mechanical requirements of skin puncture(>1 N).The drug release experiment showed that the MN could achieve gradual drug release.In the animal experiment,pigmentation and hair regrowth occurred earlier in the Polygonum multiflorum-MN(Pm-MN)group than in the other groups,and hair growth finally appeared in almost the entire area.Compared with the AGA model mice,mice in the Pm-MN group achieved an increase in the number and diameter of hair follicles.In conclusion,the Pm-MN is scientific and feasible for treating AGA.展开更多
基金supported by the National Natural Science Foundation of China (U23B2009, 62376201, 423B2104)Open Foundation (ZNXX2023MSO2, HBIR202311)。
文摘Degradation under challenging conditions such as rain, haze, and low light not only diminishes content visibility, but also results in additional degradation side effects, including detail occlusion and color distortion. However, current technologies have barely explored the correlation between perturbation removal and background restoration, consequently struggling to generate high-naturalness content in challenging scenarios. In this paper, we rethink the image enhancement task from the perspective of joint optimization: Perturbation removal and texture reconstruction. To this end, we advise an efficient yet effective image enhancement model, termed the perturbation-guided texture reconstruction network(PerTeRNet). It contains two subnetworks designed for the perturbation elimination and texture reconstruction tasks, respectively. To facilitate texture recovery,we develop a novel perturbation-guided texture enhancement module(PerTEM) to connect these two tasks, where informative background features are extracted from the input with the guidance of predicted perturbation priors. To alleviate the learning burden and computational cost, we suggest performing perturbation removal in a sub-space and exploiting super-resolution to infer high-frequency background details. Our PerTeRNet has demonstrated significant superiority over typical methods in both quantitative and qualitative measures, as evidenced by extensive experimental results on popular image enhancement and joint detection tasks. The source code is available at https://github.com/kuijiang94/PerTeRNet.
文摘Selective clearance of organelles,including endoplasmic reticulum(ER)and mitochondria,by autophagy plays an important role in cell health.Here,we describe a developmentally programmed selective ER clearance by autophagy.We show that Parkinson's disease-associated PINK1,as well as Atl,Rtnl1,and Trp1 receptors,regulate ER clearance by autophagy.
基金supported by Sanming Project of Medicine in Shenzhen(No.SZSM202011014)Shenzhen Fund for Guangdong Provincial High level Clinical Key Specialties(No.SZGSP013)Research Project of Hubei Provincial Department of Education(B2019061).
文摘Background:Although more and more attention has been paid to the psychological consequences of the lockdown policy amongst pregnant women,the underlying mechanism linking the lockdown policy to maternal depression has not been studied in the context of China.This study aimed to explore the association between the lockdown policy and maternal depressive symptoms,and whether such association was mediated by internet use and/or family support.Methods:This cross-sectional study used multi-stage sampling techniques in central and western China.Data were collected from 1266 pregnant women using a structtured questionnaire that measured internet use,family support,and depressive symptoms.The Patient Health Questionnaire-9(PHQ-9)was used to measure depressive symptoms.Internet use was measured by length of usage and varierity of purpose for internet use.Family support was measureed by spousal support and parental support.The structural equation modelling was employed to conduct mediation analysis to test the specificity of the hypothetical paths.Results:Overall,527 respondents(41.63%)presented depressive symptoms.The lockdown policy was negatively associated with depressive symptoms in pregnant women(β=−0.925,95%CI=−1.510,−0.360).The impact of the lockdown policy on depressive symptoms was partially mediated by internet use(β=1.589,95%CI=0.730,2.807)and family support(β=−0.162,95%CI=−0.341,−0.017),accounting for 42.67%of the total effect.Conclusions:The lockdown policy was generally associated with fewer depressive symptoms in pregnant women.The lockdown policy increased maternal depressive symptoms through increased internet use,but decreased maternal depressive symptoms through enhanced family support.The findings suggest that the psychological consequence of the lockdown policy may vary across different populations,and warrant the need to take into consideration the features of subgroups.
基金supported by the National Key R&D Program of China(2022YFA1104900)National Natural Science Foundation of China(no.32271409)+3 种基金the Natural Science Foundation of Shandong Province(ZR2023ZD30)the Nanjing Distinguished Youth Fund(JQX20001)the Binjiang Institute of Zhejiang University(ZY202205SMKY007)the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2022R01002).
文摘Osteosarcoma(OS)is one of the most common malignant tumors in children and young adults.As chemotherapy and other therapies are limited by low therapeutic efficiency,severe side effects and single therapeutic function,it is of high value to develop innovative therapies for precise and efficient treatment of OS.Herein,natural photo-synthetic microalgae(C.vulgaris,CV)were utilized as carriers for the chemotherapeutic agent doxorubicin(DOX)to create a multifunctional therapeutic platform(CV@DOX)for the photo-modulation of the tumor microenvi-ronment(TME)and synergistic chemo-photodynamic therapy of osteosarcoma.CV@DOX exhibited rapid drug release behavior in the acidic TME,improving the efficiency of chemotherapy against tumors and reducing side effects on other normal tissues.Under 650 nm laser irradiation,CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species(ROS),thereby enhancing photodynamic therapy(PDT).CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis,inhibiting tumor proliferation and angiogenesis.Moreover,chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after administration.Finally,CV@DOX did not cause significant hematological and tissue toxicity,and prevented DOX-induced cardiotoxicity,showing good in vivo biocompatibility.Overall,this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosar-coma treatment.
基金supported by grants from the National Natural Science Foundation of China(31871360,32022018,and 31701176 to J.G.and 31900557 to R.W.)Natural Science Foundation of Shandong Province(ZR2019PC050 to H.N.).
文摘Meiosis produces the haploid gametes required by all sexually reproducing organisms,occurring in specific temperature ranges in different organisms.However,how meiotic thermotolerance is regulated remains largely unknown.Using the model organism Caenorhabditis elegans,here,we identified the synaptonemal complex(SC)protein SYP-5 as a critical regulator of meiotic thermotolerance.syp-5-null mutants maintained a high percentage of viable progeny at 20℃ but produced significantly fewer viable progeny at 25℃,a permissive temperature in wild-type worms.Cytological analysis of meiotic events in the mutants revealed that while SC assembly and disassembly,as well as DNA double-strand break repair kinetics,were not affected by the elevated temperature,crossover designation,and bivalent formation were significantly affected.More severe homolog segregation errors were also observed at elevated temperature.A temperature switching assay revealed that late meiotic prophase events were not temperature-sensitive and that meiotic defects during pachytene stage were responsible for the reduced viability of syp-5 mutants at the elevated temperature.Moreover,SC polycomplex formation and hexanediol sensitivity analysis suggested that SYP-5 was required for the normal properties of the SC,and charge-interacting elements in SC components were involved in regulating meiotic thermotolerance.Together,these findings provide a novel molecular mechanism for meiotic thermotolerance regulation.
基金This work was financially supported by the National Natural Science Foundation of China(No.12272032)the National Key R&D Program of China(No.2020YFC0122204)the 111 Project(No.B13003).
文摘Androgenetic alopecia(AGA)is an androgen-mediated alopecia affected by both genes and hormones.Medication is a relatively common treatment.As a new drug delivery method,microneedles(MNs)can effectively break through the stratum corneum barrier,deliver drugs more efficiently,and achieve better therapeutic effects.In this study,we develop a composite double-layer MN through multi-step casting fabrication using a polydimethylsiloxane mold.The needle tip was fabricated by mixed solution of chitosan and polyvinylpyrrolidone which was loaded with Polygonum multiflorum extract,and the base layer was prepared by mixed solution of polyvinyl alcohol and polyvinylpyrrolidone.In vitro mechanical tests showed that the maximum load of a single tip of the drug-loaded MN was about 3.5 N,which met the mechanical requirements of skin puncture(>1 N).The drug release experiment showed that the MN could achieve gradual drug release.In the animal experiment,pigmentation and hair regrowth occurred earlier in the Polygonum multiflorum-MN(Pm-MN)group than in the other groups,and hair growth finally appeared in almost the entire area.Compared with the AGA model mice,mice in the Pm-MN group achieved an increase in the number and diameter of hair follicles.In conclusion,the Pm-MN is scientific and feasible for treating AGA.
