Root-knot nematodes(RKNs;Meloidogyne spp.)are a serious threat to crop production.The competition between plants and pathogens for assimilates influences the outcome of their interactions.However,the mechanisms by whi...Root-knot nematodes(RKNs;Meloidogyne spp.)are a serious threat to crop production.The competition between plants and pathogens for assimilates influences the outcome of their interactions.However,the mechanisms by which plants and nematodes compete with each other for assimilates have not been elucidated.In this study,we demonstrated that miR396a plays a negative role in defense against RKNs and a positive role in sugar accumulation in tomato roots.The overexpression of SIGRF8(Solanum lycopersicum growth-regulating factor 8),the target of miR396a,decreased the sugar content of the roots and the susceptibility to RKNs,whereas the grf8-cr mutation had the opposite effects.Furthermore,we confirmed that SIGRF8regulated the sugar content in roots by directly activating the transcription of SISTP10(Solanum lycopersicum sugar transporter protein10) in response to RKN stress.Moreover,SISTP10 was expressed primarily in the tissues surrounding giant cells,and the SISTP10knockout increased both the sugar content in the roots and the plant's susceptibility to RKNs.Overall,this study provides important insight into the molecular mechanism through which the miR396a-SIGRF8-SISTP10 module regulates sugar allocation in roots under RKN stress.展开更多
基金supported by the Beijing Natural Science Foundation(6222005,6242006)the National Natural Science Foundation of China(32002117,32372796)+3 种基金the R&D Program of Beijing Municipal Education Commission(KZ20231002033)the Beijing Municipal University classified development project(11000024T000002961733)the Beijing Innovation Consortium of Agriculture Research System(BAIC01-2024)the Science and Technology Innovation Support Program of Beijing University of Agriculture(BUA-HHXD2023).
文摘Root-knot nematodes(RKNs;Meloidogyne spp.)are a serious threat to crop production.The competition between plants and pathogens for assimilates influences the outcome of their interactions.However,the mechanisms by which plants and nematodes compete with each other for assimilates have not been elucidated.In this study,we demonstrated that miR396a plays a negative role in defense against RKNs and a positive role in sugar accumulation in tomato roots.The overexpression of SIGRF8(Solanum lycopersicum growth-regulating factor 8),the target of miR396a,decreased the sugar content of the roots and the susceptibility to RKNs,whereas the grf8-cr mutation had the opposite effects.Furthermore,we confirmed that SIGRF8regulated the sugar content in roots by directly activating the transcription of SISTP10(Solanum lycopersicum sugar transporter protein10) in response to RKN stress.Moreover,SISTP10 was expressed primarily in the tissues surrounding giant cells,and the SISTP10knockout increased both the sugar content in the roots and the plant's susceptibility to RKNs.Overall,this study provides important insight into the molecular mechanism through which the miR396a-SIGRF8-SISTP10 module regulates sugar allocation in roots under RKN stress.
