In a wheat breeding line XQ-0508 showing consistent resistance to powdery mildew disease,a recessive gene,designated PmXQ-0508,was identified and mapped to a distal region on chromosome arm 2BS.Of three resistance-ass...In a wheat breeding line XQ-0508 showing consistent resistance to powdery mildew disease,a recessive gene,designated PmXQ-0508,was identified and mapped to a distal region on chromosome arm 2BS.Of three resistance-associated genes in this region,one encoding a protein kinase was selected as the primary candidate for PmXQ-0508.Ten closely linked DNA markers developed in the study could be used for marker-assisted selection for powdery-mildew resistance in breeding programs.展开更多
It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L...It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L2425 fromthe feces of an ICU patient in China.The isolate belongs to sequence type 11 and carries the antimicrobial resistance genes blaKPC-2,bla_(CTX-M-65) and mcr-8.Through S1-nuclease pulsed-field gel electrophoresis and Southern blotting analysis,we confirmed the presence of a~110 kb plasmid harboring the mcr-8 gene with a conserved structure(inhA-mcr-8-ompR-dgkA-IS903B).Furthermore,another~130 kb plasmid carrying bla_(KPC-2) was detected along with a composite transposon flanked by insertion sequence IS26.Conjugation experiments demonstrated that both pL2425-KPC-2 and pL2425-MCR-8 plasmids were transferable.Importantly,this is the first instance of clinical fecal samples from China containing a K.pneumoniae strain carrying both blaKPC-2 and mcr-8 genes.These findings underscore the significance of surveillance for carbapenemases and mobile colistin–resistance variants.展开更多
Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage...Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.展开更多
Superspreaders are critical infectious resources in multiple infectious diseases.They can be asymptomatic or present mild symptoms but can transmit pathogens to susceptible populations,leading to severe symptoms,and e...Superspreaders are critical infectious resources in multiple infectious diseases.They can be asymptomatic or present mild symptoms but can transmit pathogens to susceptible populations,leading to severe symptoms,and even death.Early identification of this population is extremely important to inhibit the spread of infectious diseases.Right now,the whole global world is suffering from a devastating infectious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).In this article,a superspreader cluster event in coronavirus disease 2019(COVID-19)was identified by tracking contacting histories of infected patients.This cluster was found to be originated from an asymptomatic SARS-CoV-2 carrier,which resulted in 13 secondary cases getting infected.All the secondary patients presented with non-typical symptoms of COVID-19,such as fever,dry cough,and myalgia,one of which died of respiratory failure at the end.From this cluster,we learn that people with older ages,low immunity,multiple underlying diseases,especially pulmonary diseases,can contribute to a poor prognosis.Thus,asymptomatic superspreaders of COVID-19 can be extremely dangerous and must be handled time-efficiently.展开更多
基金supported by the National Natural Science Foundation of China(32072053,32301800,and 32301923)Wheat Industry Technology System of Shandong Province(SDAIT-01-01)the Key R&D Plan of Shandong Province(2022LZG002-4).
文摘In a wheat breeding line XQ-0508 showing consistent resistance to powdery mildew disease,a recessive gene,designated PmXQ-0508,was identified and mapped to a distal region on chromosome arm 2BS.Of three resistance-associated genes in this region,one encoding a protein kinase was selected as the primary candidate for PmXQ-0508.Ten closely linked DNA markers developed in the study could be used for marker-assisted selection for powdery-mildew resistance in breeding programs.
基金supported by the National Key Research and Development Programof China(2019YFC0840600,2019YFC0840609 and 2020YFE0204300)the National Natural Science Foundation of China(82072314)+3 种基金the Zhejiang Province Natural Science Foundation of China(LHDMZ22H190002 and LY19H160060)the Shandong Provincial Laboratory Project(SYS202202)the Research Project of Jinan Microecological Biomedicine Shandong Laboratory(JNL-2022011B),the Fundamental Research Funds for the Central Universities(2022ZFJH003)the CAMS Innovation Fund for Medical Sciences(2019-I2M-5-045).
文摘It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L2425 fromthe feces of an ICU patient in China.The isolate belongs to sequence type 11 and carries the antimicrobial resistance genes blaKPC-2,bla_(CTX-M-65) and mcr-8.Through S1-nuclease pulsed-field gel electrophoresis and Southern blotting analysis,we confirmed the presence of a~110 kb plasmid harboring the mcr-8 gene with a conserved structure(inhA-mcr-8-ompR-dgkA-IS903B).Furthermore,another~130 kb plasmid carrying bla_(KPC-2) was detected along with a composite transposon flanked by insertion sequence IS26.Conjugation experiments demonstrated that both pL2425-KPC-2 and pL2425-MCR-8 plasmids were transferable.Importantly,this is the first instance of clinical fecal samples from China containing a K.pneumoniae strain carrying both blaKPC-2 and mcr-8 genes.These findings underscore the significance of surveillance for carbapenemases and mobile colistin–resistance variants.
基金financially supported by National Natural Science Foundation of China(32301800,32301923 and 32072053)Wheat Industrial Technology System of Shandong Province(SDAIT-01-01)Key Research and Development Project of Shandong Province(2022LZG002-4,2023LZGC009-4-4).
文摘Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.
文摘Superspreaders are critical infectious resources in multiple infectious diseases.They can be asymptomatic or present mild symptoms but can transmit pathogens to susceptible populations,leading to severe symptoms,and even death.Early identification of this population is extremely important to inhibit the spread of infectious diseases.Right now,the whole global world is suffering from a devastating infectious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).In this article,a superspreader cluster event in coronavirus disease 2019(COVID-19)was identified by tracking contacting histories of infected patients.This cluster was found to be originated from an asymptomatic SARS-CoV-2 carrier,which resulted in 13 secondary cases getting infected.All the secondary patients presented with non-typical symptoms of COVID-19,such as fever,dry cough,and myalgia,one of which died of respiratory failure at the end.From this cluster,we learn that people with older ages,low immunity,multiple underlying diseases,especially pulmonary diseases,can contribute to a poor prognosis.Thus,asymptomatic superspreaders of COVID-19 can be extremely dangerous and must be handled time-efficiently.
