Prof.Ren ZHANG’s experience in clinical treatment of neuromyelitis optica spectrum disorders(NMOSD)is summarized in this paper.The pathogenesis of this kind of disease is complicated.Prof.Ren ZHANG treats it in both ...Prof.Ren ZHANG’s experience in clinical treatment of neuromyelitis optica spectrum disorders(NMOSD)is summarized in this paper.The pathogenesis of this kind of disease is complicated.Prof.Ren ZHANG treats it in both symptoms and root cause.In the acute stage and remission stage,based on the standard application of western medicines,acupuncture should be applied as early as possible and continued.In the acute stage,acupuncture can be an alternative and complementary treatment to reduce the adverse reaction of high dose hormone and shorten the acute course of this disease.In the remission stage,acupuncture can not only improve the visual function of patients,but also helps to retard the deterioration of this disease,reduce the recurrence and disability degree,having a long-term effect.The specific treatment method of Prof.Ren ZHANG emphasizes a combination of acupuncture and acupoint injection,with the extraordinary points as the main acupoints and coordinate with meridian acupoints in application.In the manipulation,it is emphasized deep insertion,penetrating method,and needling sensation to the diseased location.For the treatment course,it is emphasized that early intervention and long-term regular treatment.Here is the summarization of Prof.Ren ZHANG’s experience in treating this disease,which can be the reference for clinicians.展开更多
The aim of this study was to explore the impact of chronic apical periodontitis(CAP)on atherosclerosis in apoE^(−/−)mice fed high-fat diet(HFD).This investigation focused on the gut microbiota,metabolites,and intestin...The aim of this study was to explore the impact of chronic apical periodontitis(CAP)on atherosclerosis in apoE^(−/−)mice fed high-fat diet(HFD).This investigation focused on the gut microbiota,metabolites,and intestinal barrier function to uncover potential links between oral health and cardiovascular disease(CVD).In this study,CAP was shown to exacerbate atherosclerosis in HFD-fed apoE^(−/−)mice,as evidenced by the increase in plaque size and volume in the aortic walls observed via Oil Red O staining.16S rRNA sequencing revealed significant alterations in the gut microbiota,with harmful bacterial species thriving while beneficial species declining.Metabolomic profiling indicated disruptions in lipid metabolism and primary bile acid synthesis,leading to elevated levels of taurochenodeoxycholic acid(TCDCA),taurocholic acid(TCA),and tauroursodeoxycholic acid(TDCA).These metabolic shifts may contribute to atherosclerosis development.Furthermore,impaired intestinal barrier function,characterized by reduced mucin expression and disrupted tight junction proteins,was observed.The increased intestinal permeability observed was positively correlated with the severity of atherosclerotic lesions,highlighting the importance of the intestinal barrier in cardiovascular health.In conclusion,this research underscores the intricate interplay among oral health,gut microbiota composition,metabolite profiles,and CVD incidence.These findings emphasize the importance of maintaining good oral hygiene as a potential preventive measure against cardiovascular issues,as well as the need for further investigations into the intricate mechanisms linking oral health,gut microbiota,and metabolic pathways in CVD development.展开更多
The spaceborne platform has unprecedently provided the global eddy-permitting(typically about 0.25°)products of sea surface salinity(SSS),however the existing SSS products can hardly resolve mesoscale motions due...The spaceborne platform has unprecedently provided the global eddy-permitting(typically about 0.25°)products of sea surface salinity(SSS),however the existing SSS products can hardly resolve mesoscale motions due to the heavy noises therein and the over-smoothing in denoising processes.By means of the multi-fractal fusion(MFF),the high-resolution SSS product is synthesized with the template of sea surface temperature(SST).Two low-resolution SSS products and four SST products are considered as the source data and the templates respectively to determine the best combination.The fused products are validated by the in situ observations and intercompared via SSS maps,Singularity Exponent maps and wavenumber spectra.The results demonstrate that the MFF can perform a good work in mitigating the noises and improving the resolution.The combination of the climate change initiative SSS and the remote sensing system SST can produce the 0.1°denoised product whose global mean standard derivation of salinity against Argo is 0.21 and the feature resolution can reach 30−40 km.展开更多
This paper studies time-varying fault-tolerant formation tracking problems for the multiple cruise missile system under directed topologies subjected to actuator failures. Firstly, the timevarying fault-tolerant forma...This paper studies time-varying fault-tolerant formation tracking problems for the multiple cruise missile system under directed topologies subjected to actuator failures. Firstly, the timevarying fault-tolerant formation tracking process for the multiple cruise missile system is divided into the guidance loop and the control loop. Then protocols are constructed to accomplish distributed fault-tolerant formation tracking in the guidance loop with the adaptive updating mechanism, in the condition where neither the knowledge about actuator malfunctions nor any global information of the communication topology remains available. Moreover, sufficient conditions to accomplish formation tracking are presented, and it is shown that the multiple cruise missile system can carry on the predefined time-varying fault-tolerant control (FTC) formation tracking through the active disturbances rejection controller (ADRC) and the proportion integration (PI) controller by the way of the fault-tolerant protocol utilizing the designed strategies, in the event of actuator failures. At last, numerical analysis and simulation are designed to verify the theoretical results.展开更多
In this study, subsurface eddies near the Vietnam coast of the South China Sea were observed with in situ observations, including Argo, CTD, XBT and some processed and quality controlled data. Based on temperature pro...In this study, subsurface eddies near the Vietnam coast of the South China Sea were observed with in situ observations, including Argo, CTD, XBT and some processed and quality controlled data. Based on temperature profiles from four Argo floats near the coast of Vietnam, a subsurface warm eddy was identified in spring and summer. The multi-year Argo and Global Temperature and Salinity Profile Programme(GTSPP) data were merged on a seasonal basis based on the data interpolating variational analysis(DIVA) method to reconstruct the three-dimensional temperature structure. There is a warm eddy in the central subsurface at 12.5°N, 111°E below300 m depth in spring, which does not exist in autumn and is weak in winter and summer. From CSIRO Atlas of Regional Seas(CARS) and Generalized Digital Environment Model(GDEM) reanalysis data, this subsurface warm eddy is also verified in spring.展开更多
The effects of essential oil from Carpesium abrotanoides L.(CAEO)on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study.MTT assays indicated that CAEO inhibited the proliferat...The effects of essential oil from Carpesium abrotanoides L.(CAEO)on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study.MTT assays indicated that CAEO inhibited the proliferation of HCC cells with the IC50 values ranging from 41.28±3.06 to 130.36±20.79μg/mL.Moreover,many obviously nuclear morphological changes of apoptotic cells in CAEO-treated HepG2 cells were detected by Hoechst 33258staining and fluorescence microscopy.Flow cytometry was used to detect cell apoptosis and cell cycle,and noticeable findings showed that CAEO arrested cell-cycle at S and G2/M phases.The decreased Bcl-2/Bax protein ratio and the activation of caspase-3,caspase-9 were also detected by Western blotting.All results suggested that CAEO is a potential agent to fight against liver cancer,and the mitochondria-mediated intrinsic apoptotic pathway could be involved in CAEO-mediated apoptosis of human liver carcinoma cells.展开更多
Rose(Rosa hybrida)is one of most famous ornamental plants in the world,and its commodity value largely depends on its flower color.However,the regulatory mechanism underlying rose flower color is still unclear.In this...Rose(Rosa hybrida)is one of most famous ornamental plants in the world,and its commodity value largely depends on its flower color.However,the regulatory mechanism underlying rose flower color is still unclear.In this study,we found that a key R2R3-MYB transcription factor,RcMYB1,plays a central role in rose anthocyanin biosynthesis.Overexpression of RcMYB1 significantly promoted anthocyanin accumulation in both white rose petals and tobacco leaves.In 35S:RcMYB1 transgenic lines,a significant accumulation of anthocyanins occurred in leaves and petioles.We further identified two MBW complexes(RcMYB1-RcBHLH42-RcTTG1;RcMYB1-RcEGL1-RcTTG1)associated with anthocyanin accumulation.Yeast one-hybrid and luciferase assays showed that RcMYB1 could active its own gene promoter and those of other EBGs(early anthocyanin biosynthesis genes)and LBGs(late anthocyanin biosynthesis genes).In addition,both of the MBW complexes enhanced the transcriptional activity of RcMYB1 and LBGs.Interestingly,our results also indicate that RcMYB1 is involved in the metabolic regulation of carotenoids and volatile aroma.In summary,we found that RcMYB1 widely participates in the transcriptional regulation of ABGs(anthocyanin biosynthesis genes),indicative of its central role in the regulation of anthocyanin accumulation in rose.Our results provide a theoretical basis for the further improvement of the flower color trait in rose by breeding or genetic modification.展开更多
BACKGROUND Currently, the findings about irisin as a novel myokine related to obesity are inconsistent in overweight/obese people. To our knowledge, no systematic analysis has been conducted to evaluate the relationsh...BACKGROUND Currently, the findings about irisin as a novel myokine related to obesity are inconsistent in overweight/obese people. To our knowledge, no systematic analysis has been conducted to evaluate the relationship between irisin levels and overweight/obesity. AIM To evaluate the association between circulating irisin levels and overweight/obesity. METHODS The Cochrane Library, MEDLINE, SCOPUS, and the ISI Web of Science were searched to retrieve all of the studies associated with circulating irisin levels and overweight/obesity. Standard mean difference values and 95% confidence intervals (CI) were estimated and pooled using meta-analysis methodology. RESULTS A total of 18 studies were included in our meta-analysis containing 1005 cases and 1242 controls. Our analysis showed that the circulating irisin level in overweight/obese people was higher than that in overall healthy controls (random effects MD = 0.63;95%CI: 0.22-1.05;P = 0.003). In the subgroup analysis by ethnicity, the irisin level was higher in overweight/obesity people than that in controls in Africa (random effects MD = 3.41;95%CI: 1.23-5.59;P < 0.05) but not in European, Asian, or American populations. In addition, in a subgroup analysis by age, the results showed that obese children exhibited a higher irisin level than controls (random effects MD = 0.86;95%CI: 0.28-1.43;P < 0.05). CONCLUSION This meta-analysis provides evidence that circulating irisin is higher in obese individuals compared to healthy controls and it is important to identify the relationship between circulating irisin levels and overweight/obesity in predicting overweight/obesity.展开更多
Based on Bayesian network (BN) and information flow (IF),a new machine learning-based model named IFBN is put forward to interpolate missing time series of multiple ocean variables. An improved BN structural learning ...Based on Bayesian network (BN) and information flow (IF),a new machine learning-based model named IFBN is put forward to interpolate missing time series of multiple ocean variables. An improved BN structural learning algorithm with IF is designed to mine causal relationships among ocean variables to build network structure. Nondirectional inference mechanism of BN is applied to achieve the synchronous interpolation of multiple missing time series. With the IFBN,all ocean variables are placed in a causal network visually,making full use of information about related variables to fill missing data. More importantly,the synchronous interpolation of multiple variables can avoid model retraining when interpolative objects change. Interpolation experiments show that IFBN has even better interpolation accuracy,effectiveness and stability than existing methods.展开更多
Objective:To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109.Methods:The chemical composition of essential oil from Saussurea...Objective:To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109.Methods:The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry(GC-MS).The anti-proliferative,anti-migrative,and apoptotic effects of essential oil from Saussurea costus against Eca109 cells were analyzed.Moreover,the expression of proteins associated with cell cycle,metastasis,and apoptosis was determined.Results:GC-MS analysis showed that essential oil from Saussurea costus was predominantly comprised of sesquiterpenes.Saussurea costus essential oil inhibited the viability of Eca109 cells in a dose-and time-dependent manner with IC_(50) values of(24.29±1.49),(19.16±2.27)and(6.97±0.86)μg/mL at 12,24,and 48 h,respectively.The expression levels of target proteins in the cell cycle(phase G_(1)/S),including cyclin D1,p21,and p53,were affected by Saussurea costus essential oil.The essential oil also downregulated the expression of metastasis-related proteins MMP-9 and MMP-2.Moreover,it induced apoptosis of Eca109 cells through the mitochondrial pathway,as well as inhibition of STAT3 phosphorylation.Conclusions:The essential oil from Saussurea costus exhibited anti-proliferative,anti-migrative,and apoptotic effects on Eca109 cells,and could be further explored as a potential anti-esophageal cancer agent.展开更多
We study the relation between the bosonic frequency shift of a dipole oscillation and the fermionic compressibility in a Bose-Fermi super fluid mixture. Our results show that the A transition occurs in such a system, ...We study the relation between the bosonic frequency shift of a dipole oscillation and the fermionic compressibility in a Bose-Fermi super fluid mixture. Our results show that the A transition occurs in such a system, which interprets the perplexing phenomenon observed in the recent experiment [arXiv：1705.04496]. In that experiment,the frequency shift of the bosonic gas was measured with different fermion-fermion s-wave scattering lengths af. The most striking feature of their measurement is that the frequency shift shows a puzzling non-monotonic behavior around 1/kFaf（？）-0.2 with kF being the Fermi momentum. In the present work, the relation between the bosonic frequency shift and the compressibility of the Fermi gas is revealed by means of the equation of state of the super fluid mixture. Making use of the relation, we find that the compressibility of Fermi gas shows a ∧-like shape around 1/kFaf（？）-0.2. We would like to stress that no free parameter is used in our calculation and our results can be further testified in a future experiment.展开更多
We study the possibility of stabilizing a Fulde-Ferrell-Larkin-Ovchinnikov(FFLO)state in an equally populated two-component Fermi gas trapped in a moving two-dimensional optical lattice.For a system with nearly half f...We study the possibility of stabilizing a Fulde-Ferrell-Larkin-Ovchinnikov(FFLO)state in an equally populated two-component Fermi gas trapped in a moving two-dimensional optical lattice.For a system with nearly half filling,we find that a finite pairing momentum perpendicular to the moving direction can be spontaneously induced for a proper choice of lattice velocity.As a result,the total pairing momentum is tilted towards the nesting vector to take advantage of the significant enhancement of the density of states.展开更多
This paper considers robust fault detection and diagnosis for input uncertain nonlinear systems. It proposes a multi-objective fault detection criterion so that the fault residual is sensitive to the fault but insensi...This paper considers robust fault detection and diagnosis for input uncertain nonlinear systems. It proposes a multi-objective fault detection criterion so that the fault residual is sensitive to the fault but insensitive to the uncertainty as much as possible. Then the paper solves the proposed criterion by maximizing the smallest singular value of the transformation from faults to fault detection residuals while minimizing the largest singular value of the transformation from input uncertainty to the fault detection residuals. This method is applied to an aircraft which has a fault in the left elevator or rudder. The simulation results show the proposed method can detect the control surface failures rapidly and efficiently.展开更多
Small non-coding RNAs with important regulatory roles are not confined to eukaryotes. Recent work has uncovered a growing number of bacterial small RNAs （sRNAs）, some of which have been shown to regulate critical ce...Small non-coding RNAs with important regulatory roles are not confined to eukaryotes. Recent work has uncovered a growing number of bacterial small RNAs （sRNAs）, some of which have been shown to regulate critical cellular processes. Computational approaches, in combination with molecular experiments, have played an important role in the identification of these sRNAs. At present, there is no information on the presence of small non-coding RNAs and their genes in the Agrobacterium tumefaciens genome. To identify potential sRNAs in this important bacterium, deep sequencing of the short RNA populations isolated from Agrobacterium tumefaciens C58 was carried out. From a data set of more than 10,000 short sequences, 16 candidate sRNAs have been tentatively identified based on computational analysis. All of these candidates can form stem-loop structures by RNA folding predictions and the majority of the secondary structures are rich in GC base-pairs：：Some are followed by a short stretch of U residues, indicative of a rho-independent transcription terminator, whereas some of the short RNAs are found in the stem region of the hairpin, indicative of eukaryotic-like sRNAs. Experimental strategies will need to be used to verify these candidates. The study of an expanded list of candidate sRNAs in Agrobacterium will allow a more complete understanding of the range of roles played by regulatory RNAs in prokaryotes.展开更多
We show that reactive molecules with a unit probability of reaction naturally provide a simulator of some intriguing black hole physics.The unit reaction at the short distance acts as an event horizon and delivers a o...We show that reactive molecules with a unit probability of reaction naturally provide a simulator of some intriguing black hole physics.The unit reaction at the short distance acts as an event horizon and delivers a one-way traffic for matter waves passing through the potential barrier when two molecules interact by high partialwave scatterings or dipole-dipole interactions.In particular,the scattering rate as a function of the incident energy exhibits a thermal-like distribution near the maximum of the interaction energy in the same manner as a scalar field scatters with the potential barrier outside the event horizon of a black hole.Such a thermal-like scattering can be extracted from the temperature-dependent two-body loss rate measured in experiments on KRb and other molecules.展开更多
In Kriging interpolation, the types of variogram model are very finite, which make the variogram very difficult to describe the spatial distributional characteristics of true data. In order to overcome its shortage, a...In Kriging interpolation, the types of variogram model are very finite, which make the variogram very difficult to describe the spatial distributional characteristics of true data. In order to overcome its shortage, an improved interpolation called Support Vector Machine-Kriging interpolation (SVM-Kriging) was proposed in this paper. The SVM-Kriging uses Least Square Support Vector Machine (LS-SVM) to fit the variogram, which needn’t select the basic variogram model and can directly get the optimal variogram of real interpolated field by using SVM to fit the variogram curve automatically. Based on GODAS data, by using the proposed SVM-Kriging and the general Kriging based on other traditional variogram models, the interpolation test was carried out and the interpolated results were analyzed contrastively. The test show that the variogram of SVM-Kriging can avoid the subjectivity of selecting the type of variogram models and the SVM-Kriging is better than the general Kriging based on other variogram model as a whole. Therefore, the SVM-Kriging is a good and adaptive interpolation method.展开更多
Endogenous opioid peptides(EOP)are the neurochemical basis of the anesthetic and analgesic effects of acupuncture,and the quantity of acupuncture stimulus can be controlled accurately by using electroacupuncture(EA).T...Endogenous opioid peptides(EOP)are the neurochemical basis of the anesthetic and analgesic effects of acupuncture,and the quantity of acupuncture stimulus can be controlled accurately by using electroacupuncture(EA).The present study explores the dose-effect relationship between EA with different parameters and the regulation of EOP system.In this paper,the intervention effects of EA on EOP system were specially discussed in terms of the single factor and the different combinations of the frequency,waveform and current intensity.This study shows that EOP system presents a frequency-response specificity.The low frequency of EA promotes the release of enkephalin,β-endorphin and endomorphin,the high one activates the dynorphin system selectively,and the intermediate frequency works on promoting the release of enkephalin andβ-endorphin,as well as dynorphin.Sparse-dense wave of EA may induce the release of enkephalin,β-endorphin,endomorphin and dynorphin,presenting a synergistic effect.However,the waveform of EA should be selected flexibly in clinical practice.Sometimes the better therapeutic effect can also be obtained with the continuous wave of EA.EOP system is involved in mediating appropriate intensity of EA,while the acupuncture effect generated by an extra strong EA stimulation refers to a kind of stress response of non-opioid mechanism.The different combinations of EA parameters result in various effects.The combination of EA parameters should be optimized in accordance with different diseases,which is valuable for guiding clinical practice and the development of EA therapy.展开更多
基金Supported by Ren ZHANG inheritance studio of national famous senior TCM experts,National Administration of Traditional Chinese Medicine:154,296,231,683Jinshan Health Committee Jinshan District key medical specialty of health system。
文摘Prof.Ren ZHANG’s experience in clinical treatment of neuromyelitis optica spectrum disorders(NMOSD)is summarized in this paper.The pathogenesis of this kind of disease is complicated.Prof.Ren ZHANG treats it in both symptoms and root cause.In the acute stage and remission stage,based on the standard application of western medicines,acupuncture should be applied as early as possible and continued.In the acute stage,acupuncture can be an alternative and complementary treatment to reduce the adverse reaction of high dose hormone and shorten the acute course of this disease.In the remission stage,acupuncture can not only improve the visual function of patients,but also helps to retard the deterioration of this disease,reduce the recurrence and disability degree,having a long-term effect.The specific treatment method of Prof.Ren ZHANG emphasizes a combination of acupuncture and acupoint injection,with the extraordinary points as the main acupoints and coordinate with meridian acupoints in application.In the manipulation,it is emphasized deep insertion,penetrating method,and needling sensation to the diseased location.For the treatment course,it is emphasized that early intervention and long-term regular treatment.Here is the summarization of Prof.Ren ZHANG’s experience in treating this disease,which can be the reference for clinicians.
基金supported by the National Natural Science Foundation of China(No.81970926)supported by the Fujian Province Natural Science Foundation of China(No.2023J01709)the Fujian Provincial Health Technology Project(No.2022QNA073).
文摘The aim of this study was to explore the impact of chronic apical periodontitis(CAP)on atherosclerosis in apoE^(−/−)mice fed high-fat diet(HFD).This investigation focused on the gut microbiota,metabolites,and intestinal barrier function to uncover potential links between oral health and cardiovascular disease(CVD).In this study,CAP was shown to exacerbate atherosclerosis in HFD-fed apoE^(−/−)mice,as evidenced by the increase in plaque size and volume in the aortic walls observed via Oil Red O staining.16S rRNA sequencing revealed significant alterations in the gut microbiota,with harmful bacterial species thriving while beneficial species declining.Metabolomic profiling indicated disruptions in lipid metabolism and primary bile acid synthesis,leading to elevated levels of taurochenodeoxycholic acid(TCDCA),taurocholic acid(TCA),and tauroursodeoxycholic acid(TDCA).These metabolic shifts may contribute to atherosclerosis development.Furthermore,impaired intestinal barrier function,characterized by reduced mucin expression and disrupted tight junction proteins,was observed.The increased intestinal permeability observed was positively correlated with the severity of atherosclerotic lesions,highlighting the importance of the intestinal barrier in cardiovascular health.In conclusion,this research underscores the intricate interplay among oral health,gut microbiota composition,metabolite profiles,and CVD incidence.These findings emphasize the importance of maintaining good oral hygiene as a potential preventive measure against cardiovascular issues,as well as the need for further investigations into the intricate mechanisms linking oral health,gut microbiota,and metabolic pathways in CVD development.
基金The National Natural Science Foundation of China under contract Nos 42206205,41976188 and 42276205.
文摘The spaceborne platform has unprecedently provided the global eddy-permitting(typically about 0.25°)products of sea surface salinity(SSS),however the existing SSS products can hardly resolve mesoscale motions due to the heavy noises therein and the over-smoothing in denoising processes.By means of the multi-fractal fusion(MFF),the high-resolution SSS product is synthesized with the template of sea surface temperature(SST).Two low-resolution SSS products and four SST products are considered as the source data and the templates respectively to determine the best combination.The fused products are validated by the in situ observations and intercompared via SSS maps,Singularity Exponent maps and wavenumber spectra.The results demonstrate that the MFF can perform a good work in mitigating the noises and improving the resolution.The combination of the climate change initiative SSS and the remote sensing system SST can produce the 0.1°denoised product whose global mean standard derivation of salinity against Argo is 0.21 and the feature resolution can reach 30−40 km.
基金supported by the Natural Science Foundation of China(61101004 61803014)
文摘This paper studies time-varying fault-tolerant formation tracking problems for the multiple cruise missile system under directed topologies subjected to actuator failures. Firstly, the timevarying fault-tolerant formation tracking process for the multiple cruise missile system is divided into the guidance loop and the control loop. Then protocols are constructed to accomplish distributed fault-tolerant formation tracking in the guidance loop with the adaptive updating mechanism, in the condition where neither the knowledge about actuator malfunctions nor any global information of the communication topology remains available. Moreover, sufficient conditions to accomplish formation tracking are presented, and it is shown that the multiple cruise missile system can carry on the predefined time-varying fault-tolerant control (FTC) formation tracking through the active disturbances rejection controller (ADRC) and the proportion integration (PI) controller by the way of the fault-tolerant protocol utilizing the designed strategies, in the event of actuator failures. At last, numerical analysis and simulation are designed to verify the theoretical results.
基金The National Natural Science Foundation of China under contract Nos 91428206,41206002,41706021 and41775053the China Postdoctoral Science Foundation under contract No.2014M551711the Strategic Priority Research Program of the Chinese Academy of Sciences under contract No.XDA11010103
文摘In this study, subsurface eddies near the Vietnam coast of the South China Sea were observed with in situ observations, including Argo, CTD, XBT and some processed and quality controlled data. Based on temperature profiles from four Argo floats near the coast of Vietnam, a subsurface warm eddy was identified in spring and summer. The multi-year Argo and Global Temperature and Salinity Profile Programme(GTSPP) data were merged on a seasonal basis based on the data interpolating variational analysis(DIVA) method to reconstruct the three-dimensional temperature structure. There is a warm eddy in the central subsurface at 12.5°N, 111°E below300 m depth in spring, which does not exist in autumn and is weak in winter and summer. From CSIRO Atlas of Regional Seas(CARS) and Generalized Digital Environment Model(GDEM) reanalysis data, this subsurface warm eddy is also verified in spring.
基金the Natural Science Foundation of Hubei Province of China(No.2017CFB517)the Talent Introduction Project of Hangzhou Medical College,Zhejiang Province,China (No.2015B08)+2 种基金Open Project Fund of Hubei Province Key Laboratory of Occupational Hazard Identification and Control(No.OHIC2017G03)the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities(CZP17060)National Natural Science Foundation of China(No.81573239,No.31671092 and No.81773264).
文摘The effects of essential oil from Carpesium abrotanoides L.(CAEO)on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study.MTT assays indicated that CAEO inhibited the proliferation of HCC cells with the IC50 values ranging from 41.28±3.06 to 130.36±20.79μg/mL.Moreover,many obviously nuclear morphological changes of apoptotic cells in CAEO-treated HepG2 cells were detected by Hoechst 33258staining and fluorescence microscopy.Flow cytometry was used to detect cell apoptosis and cell cycle,and noticeable findings showed that CAEO arrested cell-cycle at S and G2/M phases.The decreased Bcl-2/Bax protein ratio and the activation of caspase-3,caspase-9 were also detected by Western blotting.All results suggested that CAEO is a potential agent to fight against liver cancer,and the mitochondria-mediated intrinsic apoptotic pathway could be involved in CAEO-mediated apoptosis of human liver carcinoma cells.
基金This work was supported by Shanghai Special Project of Capacity Construction for Local Colleges and Universities,No.20070502500Shanghai Science and Technology Agriculture Program,No.2022-02-08-00-12-F01146+1 种基金Science and Technology Commission of Shanghai Municipality,No.18DZ2260500Shanghai Plant Germplasm Resources Engineering Research Center,17DZ2252700.
文摘Rose(Rosa hybrida)is one of most famous ornamental plants in the world,and its commodity value largely depends on its flower color.However,the regulatory mechanism underlying rose flower color is still unclear.In this study,we found that a key R2R3-MYB transcription factor,RcMYB1,plays a central role in rose anthocyanin biosynthesis.Overexpression of RcMYB1 significantly promoted anthocyanin accumulation in both white rose petals and tobacco leaves.In 35S:RcMYB1 transgenic lines,a significant accumulation of anthocyanins occurred in leaves and petioles.We further identified two MBW complexes(RcMYB1-RcBHLH42-RcTTG1;RcMYB1-RcEGL1-RcTTG1)associated with anthocyanin accumulation.Yeast one-hybrid and luciferase assays showed that RcMYB1 could active its own gene promoter and those of other EBGs(early anthocyanin biosynthesis genes)and LBGs(late anthocyanin biosynthesis genes).In addition,both of the MBW complexes enhanced the transcriptional activity of RcMYB1 and LBGs.Interestingly,our results also indicate that RcMYB1 is involved in the metabolic regulation of carotenoids and volatile aroma.In summary,we found that RcMYB1 widely participates in the transcriptional regulation of ABGs(anthocyanin biosynthesis genes),indicative of its central role in the regulation of anthocyanin accumulation in rose.Our results provide a theoretical basis for the further improvement of the flower color trait in rose by breeding or genetic modification.
基金Supported by the National Natural Science Foundation of China,No.81500351the Youth Medical Talent Project of Jiangsu Province,No.QNRC2016842+3 种基金the Jiangsu University Affiliated Hospital"5123"Talent Plan,No.51232017305the 169 Talent Project of Zhenjiang
文摘BACKGROUND Currently, the findings about irisin as a novel myokine related to obesity are inconsistent in overweight/obese people. To our knowledge, no systematic analysis has been conducted to evaluate the relationship between irisin levels and overweight/obesity. AIM To evaluate the association between circulating irisin levels and overweight/obesity. METHODS The Cochrane Library, MEDLINE, SCOPUS, and the ISI Web of Science were searched to retrieve all of the studies associated with circulating irisin levels and overweight/obesity. Standard mean difference values and 95% confidence intervals (CI) were estimated and pooled using meta-analysis methodology. RESULTS A total of 18 studies were included in our meta-analysis containing 1005 cases and 1242 controls. Our analysis showed that the circulating irisin level in overweight/obese people was higher than that in overall healthy controls (random effects MD = 0.63;95%CI: 0.22-1.05;P = 0.003). In the subgroup analysis by ethnicity, the irisin level was higher in overweight/obesity people than that in controls in Africa (random effects MD = 3.41;95%CI: 1.23-5.59;P < 0.05) but not in European, Asian, or American populations. In addition, in a subgroup analysis by age, the results showed that obese children exhibited a higher irisin level than controls (random effects MD = 0.86;95%CI: 0.28-1.43;P < 0.05). CONCLUSION This meta-analysis provides evidence that circulating irisin is higher in obese individuals compared to healthy controls and it is important to identify the relationship between circulating irisin levels and overweight/obesity in predicting overweight/obesity.
基金The National Natural Science Foundation of China under contract Nos 41875061 and 41976188the“Double First-Class”Research Program of National University of Defense Technology under contract No.xslw05.
文摘Based on Bayesian network (BN) and information flow (IF),a new machine learning-based model named IFBN is put forward to interpolate missing time series of multiple ocean variables. An improved BN structural learning algorithm with IF is designed to mine causal relationships among ocean variables to build network structure. Nondirectional inference mechanism of BN is applied to achieve the synchronous interpolation of multiple missing time series. With the IFBN,all ocean variables are placed in a causal network visually,making full use of information about related variables to fill missing data. More importantly,the synchronous interpolation of multiple variables can avoid model retraining when interpolative objects change. Interpolation experiments show that IFBN has even better interpolation accuracy,effectiveness and stability than existing methods.
基金financially supported by the Scientific Research and Innovation Fund of Wuhan Asia General Hospital (Project No. WAGH-DSE-2022-005)2020 General Planning Fund Project for Humanities and Social Sciences of the Ministry of Education,China (Project No. 20YJA880053)+3 种基金Key Research Project of Philosophy and Social Sciences of Hubei Provincial Department of Education in 2020 (Project No. 20D026)WUST National Defence Pre-research Foundation,China (Project No. GF202003)Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology (Project No. OHIC2020G05)National Undergraduate Innovation and Entrepreneurship Training Project (Project No. JCX201976)
文摘Objective:To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109.Methods:The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry(GC-MS).The anti-proliferative,anti-migrative,and apoptotic effects of essential oil from Saussurea costus against Eca109 cells were analyzed.Moreover,the expression of proteins associated with cell cycle,metastasis,and apoptosis was determined.Results:GC-MS analysis showed that essential oil from Saussurea costus was predominantly comprised of sesquiterpenes.Saussurea costus essential oil inhibited the viability of Eca109 cells in a dose-and time-dependent manner with IC_(50) values of(24.29±1.49),(19.16±2.27)and(6.97±0.86)μg/mL at 12,24,and 48 h,respectively.The expression levels of target proteins in the cell cycle(phase G_(1)/S),including cyclin D1,p21,and p53,were affected by Saussurea costus essential oil.The essential oil also downregulated the expression of metastasis-related proteins MMP-9 and MMP-2.Moreover,it induced apoptosis of Eca109 cells through the mitochondrial pathway,as well as inhibition of STAT3 phosphorylation.Conclusions:The essential oil from Saussurea costus exhibited anti-proliferative,anti-migrative,and apoptotic effects on Eca109 cells,and could be further explored as a potential anti-esophageal cancer agent.
文摘We study the relation between the bosonic frequency shift of a dipole oscillation and the fermionic compressibility in a Bose-Fermi super fluid mixture. Our results show that the A transition occurs in such a system, which interprets the perplexing phenomenon observed in the recent experiment [arXiv：1705.04496]. In that experiment,the frequency shift of the bosonic gas was measured with different fermion-fermion s-wave scattering lengths af. The most striking feature of their measurement is that the frequency shift shows a puzzling non-monotonic behavior around 1/kFaf（？）-0.2 with kF being the Fermi momentum. In the present work, the relation between the bosonic frequency shift and the compressibility of the Fermi gas is revealed by means of the equation of state of the super fluid mixture. Making use of the relation, we find that the compressibility of Fermi gas shows a ∧-like shape around 1/kFaf（？）-0.2. We would like to stress that no free parameter is used in our calculation and our results can be further testified in a future experiment.
基金supported by the Beijing Natural Science Foundation,China(Grant No.Z180013)the National Natural Science Foundation of China(Grant Nos.11522436,11774425,and 12074428)+1 种基金the National Key R&D Program of China(Grant No.2018YFA0306501)the Research Funds of Renmin University of China(Grant Nos.16XNLQ03 and 18XNLQ15)。
文摘We study the possibility of stabilizing a Fulde-Ferrell-Larkin-Ovchinnikov(FFLO)state in an equally populated two-component Fermi gas trapped in a moving two-dimensional optical lattice.For a system with nearly half filling,we find that a finite pairing momentum perpendicular to the moving direction can be spontaneously induced for a proper choice of lattice velocity.As a result,the total pairing momentum is tilted towards the nesting vector to take advantage of the significant enhancement of the density of states.
基金supported by the National Natural Science Foundation of China(60328304)the"111"project of Beihang University (B07009)
文摘This paper considers robust fault detection and diagnosis for input uncertain nonlinear systems. It proposes a multi-objective fault detection criterion so that the fault residual is sensitive to the fault but insensitive to the uncertainty as much as possible. Then the paper solves the proposed criterion by maximizing the smallest singular value of the transformation from faults to fault detection residuals while minimizing the largest singular value of the transformation from input uncertainty to the fault detection residuals. This method is applied to an aircraft which has a fault in the left elevator or rudder. The simulation results show the proposed method can detect the control surface failures rapidly and efficiently.
文摘Small non-coding RNAs with important regulatory roles are not confined to eukaryotes. Recent work has uncovered a growing number of bacterial small RNAs （sRNAs）, some of which have been shown to regulate critical cellular processes. Computational approaches, in combination with molecular experiments, have played an important role in the identification of these sRNAs. At present, there is no information on the presence of small non-coding RNAs and their genes in the Agrobacterium tumefaciens genome. To identify potential sRNAs in this important bacterium, deep sequencing of the short RNA populations isolated from Agrobacterium tumefaciens C58 was carried out. From a data set of more than 10,000 short sequences, 16 candidate sRNAs have been tentatively identified based on computational analysis. All of these candidates can form stem-loop structures by RNA folding predictions and the majority of the secondary structures are rich in GC base-pairs：：Some are followed by a short stretch of U residues, indicative of a rho-independent transcription terminator, whereas some of the short RNAs are found in the stem region of the hairpin, indicative of eukaryotic-like sRNAs. Experimental strategies will need to be used to verify these candidates. The study of an expanded list of candidate sRNAs in Agrobacterium will allow a more complete understanding of the range of roles played by regulatory RNAs in prokaryotes.
基金the National Natural Science Foundation of China(Grant No.12174300)the National Key R&D Program of China(Grant No.2018YFA0307601)supported by the National Science Foundation(Grant No.PHY-2110614)。
文摘We show that reactive molecules with a unit probability of reaction naturally provide a simulator of some intriguing black hole physics.The unit reaction at the short distance acts as an event horizon and delivers a one-way traffic for matter waves passing through the potential barrier when two molecules interact by high partialwave scatterings or dipole-dipole interactions.In particular,the scattering rate as a function of the incident energy exhibits a thermal-like distribution near the maximum of the interaction energy in the same manner as a scalar field scatters with the potential barrier outside the event horizon of a black hole.Such a thermal-like scattering can be extracted from the temperature-dependent two-body loss rate measured in experiments on KRb and other molecules.
文摘In Kriging interpolation, the types of variogram model are very finite, which make the variogram very difficult to describe the spatial distributional characteristics of true data. In order to overcome its shortage, an improved interpolation called Support Vector Machine-Kriging interpolation (SVM-Kriging) was proposed in this paper. The SVM-Kriging uses Least Square Support Vector Machine (LS-SVM) to fit the variogram, which needn’t select the basic variogram model and can directly get the optimal variogram of real interpolated field by using SVM to fit the variogram curve automatically. Based on GODAS data, by using the proposed SVM-Kriging and the general Kriging based on other traditional variogram models, the interpolation test was carried out and the interpolated results were analyzed contrastively. The test show that the variogram of SVM-Kriging can avoid the subjectivity of selecting the type of variogram models and the SVM-Kriging is better than the general Kriging based on other variogram model as a whole. Therefore, the SVM-Kriging is a good and adaptive interpolation method.
基金Supported by the National Natural Science Foundation of China:No.815740786th Cycle Medical Key Specialty Construction Project of Jinshan District:JSZK2019H04Three Years Action Plan of Shanghai to Further Accelerate the Development of Traditional Chinese Medicine:2018-2020。
文摘Endogenous opioid peptides(EOP)are the neurochemical basis of the anesthetic and analgesic effects of acupuncture,and the quantity of acupuncture stimulus can be controlled accurately by using electroacupuncture(EA).The present study explores the dose-effect relationship between EA with different parameters and the regulation of EOP system.In this paper,the intervention effects of EA on EOP system were specially discussed in terms of the single factor and the different combinations of the frequency,waveform and current intensity.This study shows that EOP system presents a frequency-response specificity.The low frequency of EA promotes the release of enkephalin,β-endorphin and endomorphin,the high one activates the dynorphin system selectively,and the intermediate frequency works on promoting the release of enkephalin andβ-endorphin,as well as dynorphin.Sparse-dense wave of EA may induce the release of enkephalin,β-endorphin,endomorphin and dynorphin,presenting a synergistic effect.However,the waveform of EA should be selected flexibly in clinical practice.Sometimes the better therapeutic effect can also be obtained with the continuous wave of EA.EOP system is involved in mediating appropriate intensity of EA,while the acupuncture effect generated by an extra strong EA stimulation refers to a kind of stress response of non-opioid mechanism.The different combinations of EA parameters result in various effects.The combination of EA parameters should be optimized in accordance with different diseases,which is valuable for guiding clinical practice and the development of EA therapy.
