Mitochondria,the most crucial energy-generating organelles in eukaryotic cells,play a pivotal role in regulating energy metabolism.However,their significance extends beyond this,as they are also indispensable in vital...Mitochondria,the most crucial energy-generating organelles in eukaryotic cells,play a pivotal role in regulating energy metabolism.However,their significance extends beyond this,as they are also indispensable in vital life processes such as cell proliferation,differentiation,immune responses,and redox balance.In response to various physiological signals or external stimuli,a sophisticated mitochondrial quality control(MQC)mechanism has evolved,encompassing key processes like mitochondrial biogenesis,mitochondrial dynamics,and mitophagy,which have garnered increasing attention from researchers to unveil their specific molecular mechanisms.In this review,we present a comprehensive summary of the primary mechanisms and functions of key regulators involved in major components of MQC.Furthermore,the critical physiological functions regulated by MQC and its diverse roles in the progression of various systemic diseases have been described in detail.We also discuss agonists or antagonists targeting MQC,aiming to explore potential therapeutic and research prospects by enhancing MQC to stabilize mitochondrial function.展开更多
The number of patients developing esophageal cancer after gastrectomy has increased.However,gastric remnant is very rarely used for reconstruction in esophageal cancer surgery because of the risk of anastomotic leakag...The number of patients developing esophageal cancer after gastrectomy has increased.However,gastric remnant is very rarely used for reconstruction in esophageal cancer surgery because of the risk of anastomotic leakage resulting from insufficient blood flow.We present a case of esophageal cancer using gastric remnant for esophageal substitution after distal gastrectomy in a 57-year-old man who presented with a 1-month history of mild dysphagia and a background history of alcohol abuse.Gastroscopy showed a 1.2 cm × 1.0 cm bulge tumor of the lower third esophagus with the upper margin located 39 cm from the dental arcade.Computed tomography of the chest showed lower third esophageal wall thickening.The patient underwent en bloc radical esophagectomy with a two-field lymph node dissection of the upper abdomen and mediastinum via a left-sided posterolateral thoracotomy through the seventh intercostal space.The upper end of the esophagus was resected 5 cm above the tumor.The gastric remnant was used for reconstruction of the esophago-gastrostomy and placed in the left thoracic cavity.The patient started a liquid diet on postoperative day 8 and was discharged on the 10 th postoperative day without complications.In this report,we demonstrate that the gastric remnant may be used for reconstruction in patients with esophageal cancer as a substitute organ after distal gastrectomy.展开更多
The colon is an alternative graft organ for esophageal reconstruction.The present study reviewed our experience with the colon interposition for esophageal replacement following corrosive ingestion,to evaluate the out...The colon is an alternative graft organ for esophageal reconstruction.The present study reviewed our experience with the colon interposition for esophageal replacement following corrosive ingestion,to evaluate the outcomes of colon interposition based on our surgical experience. The clinical data of 119 patients who underwent colon interposition for esophageal replacement from January 2005 to March 2017 were retrospectively analyzed. The routes of the colon interposition were retrosternal in 119 (100%). The median operative time was 390 min (range: 290-610 min) and the median blood loss was 615 mL (range:270-2500 mL). Of these 119 patients, the cervical anastomosis was performed at the hypopharynx (n=20,16.8%), the larynx (n=3,2.5%), and the cervical esophagus (n=96, 80.7%). Five patients experienced cervical anastomotic leakage (4 cases for esophagus-colon, and one for hypopharynx-colon).One patient experienced wound infection of the abdominal wall. Three patients had injury of recurrent laryngeal nerve and hoarseness. Three patients had stress ulcer with bleeding and treated with octreotide. Two patients suffered from incomplete intestinal obstruction. The postoperative follow-up was made for 12 months in all patients and all of them were alive. In conclusion, The colon is well-suited for esophageal reconstruction. The selection of the colon graft should be flexible and be based on the inspection of blood supply and the length needed. We must therefore make every effort to reduce the number of postoperative complications, and improve the quality of life for patients.展开更多
Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sens...Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sensing(CS)makes it practically applicable by significantly reducing the data volume while maintaining its highspeed and high-quality imaging properties.To enrich the information of the images acquired with CS-equipped OTS imaging flow cytometry,in this work we propose and experimentally demonstrate Fourier-domaincompressed OTS quantitative phase imaging flow cytometry.It is capable of acquiring intensity and quantitative phase images of cells simultaneously from the compressed data.To evaluate the performance of our method,static microparticles and a corn root cross section are experimentally measured under various compression ratios.Furthermore,to show how our method can be applied in practice,we utilize it in the drug response analysis of breast cancer cells.Experimental results show that our method can acquire high-quality intensity and quantitative phase images of flowing cells at a flowing speed of 1 m/s and a compression ratio of 30%.Combined with machine-learning-based image analysis,it can distinguish drug-treated and drug-untreated cells with an accuracy of over 95%.We believe our method can facilitate cell analysis in both scientific research and clinical settings where both high-throughput and high-content cell analysis is required.展开更多
Dear Editor,Coronaviruses (Co Vs) are large enveloped non-segmented positive-strand RNA viruses that broadly distribute among humans and other animal species, including bats, mice and birds. SARS-Co V-2 infections can...Dear Editor,Coronaviruses (Co Vs) are large enveloped non-segmented positive-strand RNA viruses that broadly distribute among humans and other animal species, including bats, mice and birds. SARS-Co V-2 infections can cause diseases, named the 2019 novel coronavirus disease (COVID-19). The symptoms of COVID-19 range from mild symptoms to severe respiratory syndromes, including pneumonia, and even death(Chen et al., 2020b;Jiang and Shi, 2020;Xia et al., 2020).展开更多
Dear Editor,The molecular pathogenesis of estrogen receptor(ER)-negative,especially triple-negative breast cancer(TNBC),is unclear,thus,the treatment of these types of cancers is still a great challenge[1].Chemotherap...Dear Editor,The molecular pathogenesis of estrogen receptor(ER)-negative,especially triple-negative breast cancer(TNBC),is unclear,thus,the treatment of these types of cancers is still a great challenge[1].Chemotherapy remains the primary adjuvant treatment choice for TNBC patients[2],but a large number of these tumors are resistant to chemotherapy and relapse ormetastasize quickly after adjuvant therapy[3].展开更多
The world’s first porcine-to-human heart transplantation was performed at the University of Maryland School of Medicine(Baltimore,MD,USA),where a genetically modified pig heart was successfully transplanted into a 57...The world’s first porcine-to-human heart transplantation was performed at the University of Maryland School of Medicine(Baltimore,MD,USA),where a genetically modified pig heart was successfully transplanted into a 57-year-oldman in the end stage of heart disease.After highly experimental surgery,the patient was able to move about freely in the absence of cardiopulmonary bypass assistance.展开更多
基金supported by the National Natural Science Foundation of China(8210082163,81800343)the Fundamental Research Fund for the Central Universities(2042021kf0081)+1 种基金the Science Fund for Creative Research Groups of the Natural Science Foundation of Hubei Province(2020CFA027)the Doctor of Excellence Program of the First Hospital of Jilin University(JDYY-DEP-2023043).
文摘Mitochondria,the most crucial energy-generating organelles in eukaryotic cells,play a pivotal role in regulating energy metabolism.However,their significance extends beyond this,as they are also indispensable in vital life processes such as cell proliferation,differentiation,immune responses,and redox balance.In response to various physiological signals or external stimuli,a sophisticated mitochondrial quality control(MQC)mechanism has evolved,encompassing key processes like mitochondrial biogenesis,mitochondrial dynamics,and mitophagy,which have garnered increasing attention from researchers to unveil their specific molecular mechanisms.In this review,we present a comprehensive summary of the primary mechanisms and functions of key regulators involved in major components of MQC.Furthermore,the critical physiological functions regulated by MQC and its diverse roles in the progression of various systemic diseases have been described in detail.We also discuss agonists or antagonists targeting MQC,aiming to explore potential therapeutic and research prospects by enhancing MQC to stabilize mitochondrial function.
文摘The number of patients developing esophageal cancer after gastrectomy has increased.However,gastric remnant is very rarely used for reconstruction in esophageal cancer surgery because of the risk of anastomotic leakage resulting from insufficient blood flow.We present a case of esophageal cancer using gastric remnant for esophageal substitution after distal gastrectomy in a 57-year-old man who presented with a 1-month history of mild dysphagia and a background history of alcohol abuse.Gastroscopy showed a 1.2 cm × 1.0 cm bulge tumor of the lower third esophagus with the upper margin located 39 cm from the dental arcade.Computed tomography of the chest showed lower third esophageal wall thickening.The patient underwent en bloc radical esophagectomy with a two-field lymph node dissection of the upper abdomen and mediastinum via a left-sided posterolateral thoracotomy through the seventh intercostal space.The upper end of the esophagus was resected 5 cm above the tumor.The gastric remnant was used for reconstruction of the esophago-gastrostomy and placed in the left thoracic cavity.The patient started a liquid diet on postoperative day 8 and was discharged on the 10 th postoperative day without complications.In this report,we demonstrate that the gastric remnant may be used for reconstruction in patients with esophageal cancer as a substitute organ after distal gastrectomy.
文摘The colon is an alternative graft organ for esophageal reconstruction.The present study reviewed our experience with the colon interposition for esophageal replacement following corrosive ingestion,to evaluate the outcomes of colon interposition based on our surgical experience. The clinical data of 119 patients who underwent colon interposition for esophageal replacement from January 2005 to March 2017 were retrospectively analyzed. The routes of the colon interposition were retrosternal in 119 (100%). The median operative time was 390 min (range: 290-610 min) and the median blood loss was 615 mL (range:270-2500 mL). Of these 119 patients, the cervical anastomosis was performed at the hypopharynx (n=20,16.8%), the larynx (n=3,2.5%), and the cervical esophagus (n=96, 80.7%). Five patients experienced cervical anastomotic leakage (4 cases for esophagus-colon, and one for hypopharynx-colon).One patient experienced wound infection of the abdominal wall. Three patients had injury of recurrent laryngeal nerve and hoarseness. Three patients had stress ulcer with bleeding and treated with octreotide. Two patients suffered from incomplete intestinal obstruction. The postoperative follow-up was made for 12 months in all patients and all of them were alive. In conclusion, The colon is well-suited for esophageal reconstruction. The selection of the colon graft should be flexible and be based on the inspection of blood supply and the length needed. We must therefore make every effort to reduce the number of postoperative complications, and improve the quality of life for patients.
基金National Key Research and Development Program of China(2023YFF0723300)Fundamental Research Funds for the Central Universities(2042023kf0105,2042024kf0003,2042024kf1010)+6 种基金Science Fund for Distinguished Young Scholars of Hubei Province(2021CFA042)Natural Science Foundation of Hubei Province(2023AFB133)National Natural Science Foundation of China(12374295,62075200)Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCYR-2022-006)Jiangsu Science and Technology Program(BK20221257)Shenzhen Science and Technology Program(JCYJ20220530140601003,JCYJ20230807090207014)Translational Medicine and Multidisciplinary Research Project of Zhongnan Hospital of Wuhan University(ZNJC202217)。
文摘Optical time-stretch(OTS)imaging flow cytometry offers a promising solution for high-throughput and highprecision cell analysis due to its capabilities of high-speed,high-quality,and continuous imaging.Compressed sensing(CS)makes it practically applicable by significantly reducing the data volume while maintaining its highspeed and high-quality imaging properties.To enrich the information of the images acquired with CS-equipped OTS imaging flow cytometry,in this work we propose and experimentally demonstrate Fourier-domaincompressed OTS quantitative phase imaging flow cytometry.It is capable of acquiring intensity and quantitative phase images of cells simultaneously from the compressed data.To evaluate the performance of our method,static microparticles and a corn root cross section are experimentally measured under various compression ratios.Furthermore,to show how our method can be applied in practice,we utilize it in the drug response analysis of breast cancer cells.Experimental results show that our method can acquire high-quality intensity and quantitative phase images of flowing cells at a flowing speed of 1 m/s and a compression ratio of 30%.Combined with machine-learning-based image analysis,it can distinguish drug-treated and drug-untreated cells with an accuracy of over 95%.We believe our method can facilitate cell analysis in both scientific research and clinical settings where both high-throughput and high-content cell analysis is required.
基金supported by the Strategic Priority Research Program of CAS(XDB29010300 to X.Z.)the National Natural Science Foundation of China(31800140 to J.M.,81873964 to Y.Q.,and 31670161 to X.Z.)+1 种基金the National Science and Technology Major Project(2018ZX10101004 to X.Z.)the Yunde Hou Academician Fund from National Institute for Viral Disease Control and Prevention(2019HYDQNJJ10 to J.M.)。
文摘Dear Editor,Coronaviruses (Co Vs) are large enveloped non-segmented positive-strand RNA viruses that broadly distribute among humans and other animal species, including bats, mice and birds. SARS-Co V-2 infections can cause diseases, named the 2019 novel coronavirus disease (COVID-19). The symptoms of COVID-19 range from mild symptoms to severe respiratory syndromes, including pneumonia, and even death(Chen et al., 2020b;Jiang and Shi, 2020;Xia et al., 2020).
基金supported in part by NIH-NIMHD U54MD007598,U54CA143931,NIH/NCI1U54CA14393,U56 CA101599-01Department-of-Defense Breast Cancer Research Program grant BC043180,NIH/NCATS CTSI UL1TR000124 to J.V.Vadgama+1 种基金Accelerating Excellence in Translational Science(AXIS)Pilot Grants G0812D05,NIH/NCI SC1CA200517 and 9 SC1 GM135050-05 to Y.WuNIH/NIMHD Accelerating Excellence in Translational Science(AXIS)Pilot Grants G0814C01(Q.Hao).
文摘Dear Editor,The molecular pathogenesis of estrogen receptor(ER)-negative,especially triple-negative breast cancer(TNBC),is unclear,thus,the treatment of these types of cancers is still a great challenge[1].Chemotherapy remains the primary adjuvant treatment choice for TNBC patients[2],but a large number of these tumors are resistant to chemotherapy and relapse ormetastasize quickly after adjuvant therapy[3].
文摘The world’s first porcine-to-human heart transplantation was performed at the University of Maryland School of Medicine(Baltimore,MD,USA),where a genetically modified pig heart was successfully transplanted into a 57-year-oldman in the end stage of heart disease.After highly experimental surgery,the patient was able to move about freely in the absence of cardiopulmonary bypass assistance.
