The quality traits of horticultural crops,including the accumulation of nutrients and flavor substances,morphology,and texture,affect the palatability and nutritional value.For many years,efforts have been made to imp...The quality traits of horticultural crops,including the accumulation of nutrients and flavor substances,morphology,and texture,affect the palatability and nutritional value.For many years,efforts have been made to improve the quality of horticultural crops.The recent establishment of gene editing technology,with its potential applications in horticultural crops,provides a strategy for achieving this goal in a rapid and efficient manner.Here,we summarize research efforts aimed at improving horticultural crop quality through genome editing.We describe specific genome editing systems that have been used and traits that have been targeted in these efforts.Additionally,we discuss limiting factors and future perspectives of genome editing technology in improving horticultural crop qualities in both research and plant breeding.In summary,genome editing technology is emerging as a powerful tool for efficiently and rapidly improving horticultural crop quality,and we believe that the cautious application of genome editing in horticultural crops will generate new germplasms with improved quality in the near future.展开更多
The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberr...The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.展开更多
In recent years,China’s outward foreign direct investment(OFDI)has risen significantly,arousing considerable interest in the motivations and drivers of Chinese overseas investment.This paper selected 27 host country-...In recent years,China’s outward foreign direct investment(OFDI)has risen significantly,arousing considerable interest in the motivations and drivers of Chinese overseas investment.This paper selected 27 host country-related indicators and extracted the common factors using the factor analysis method.This paper discusses the determinants of China’s OFDI location choice by using panel data regression method,and focuses on the differences between Belt and Road countries and nonBelt and Road countries.The results show that the favorable institutional environment and strong market demand of host countries have a positive influence on Chinese foreign investment.Besides,China’s investment in Belt and Road Initiative countries is more prone to a country with less developed technology and unreasonable energy utilization.China’s OFDI can promote technological progress in these countries and making full use of their advantage resources for economic development.As China’s economy has entered a“new normal”,its global influence has risen,and the relationship between the host countries and China has also mattered more on China’s OFDI decisions.This effort provides important supports for optimizing the location choices of Chinese enterprises’foreign investment.展开更多
基金This work was supported by the National Key Research and Development Program(Grant No.2018YFD1000200)the National Natural Science Foundation of China(Grant No.31772284).
文摘The quality traits of horticultural crops,including the accumulation of nutrients and flavor substances,morphology,and texture,affect the palatability and nutritional value.For many years,efforts have been made to improve the quality of horticultural crops.The recent establishment of gene editing technology,with its potential applications in horticultural crops,provides a strategy for achieving this goal in a rapid and efficient manner.Here,we summarize research efforts aimed at improving horticultural crop quality through genome editing.We describe specific genome editing systems that have been used and traits that have been targeted in these efforts.Additionally,we discuss limiting factors and future perspectives of genome editing technology in improving horticultural crop qualities in both research and plant breeding.In summary,genome editing technology is emerging as a powerful tool for efficiently and rapidly improving horticultural crop quality,and we believe that the cautious application of genome editing in horticultural crops will generate new germplasms with improved quality in the near future.
基金Thisworkwas supported by the National Natural Science Foundation of China(32222074,32072551)the National Key Research and Development Program of China(2022YFD2100102-3)+2 种基金111 Project(B17043)Beijing Innovation Consortium of Agriculture Research System(BAIC04-2022)the 2115 Talent Development Program of China Agricultural University.
文摘The WRKY transcription factors play important roles in plant growth and resistance,but only a few members have been identified in strawberry.Here we identified a WRKY transcription factor,FvWRKY50,in diploid strawberry which played essential roles in strawberry vegetative growth,and reproductive growth.Knocking out FvWRKY50 by genome editing accelerated flowering time and leaf senescence but delayed anthocyanin accumulation in fruit.Further analysis showed that FvWRKY50 acted as a transcriptional repressor to negatively regulate the expression of flowering-and leaf senescence-related genes,including FvFT2,FvCO,FvFT3,and FvSAUR36.Notably,FvWRKY50 directly upregulated the expression of FvCHI and FvDFR by binding their promoter under normal conditions,but at low temperature FvWRKY50 was phosphorylated by FvMAPK3 and then induced protein degradation by ubiquitination,delaying anthocyanin accumulation.In addition,the homozygous mutant of FvWRKY50 was smaller while the biallelic mutant showed normal size.These new findings provide important clues for us to further reveal the regulatory mechanisms of strawberry growth and fruit ripening.
基金Supported by the Social and Science Fund of Xinjiang (17BKS008)General Project of Inner-Party Law Research Center (XJDG2019D02)
文摘In recent years,China’s outward foreign direct investment(OFDI)has risen significantly,arousing considerable interest in the motivations and drivers of Chinese overseas investment.This paper selected 27 host country-related indicators and extracted the common factors using the factor analysis method.This paper discusses the determinants of China’s OFDI location choice by using panel data regression method,and focuses on the differences between Belt and Road countries and nonBelt and Road countries.The results show that the favorable institutional environment and strong market demand of host countries have a positive influence on Chinese foreign investment.Besides,China’s investment in Belt and Road Initiative countries is more prone to a country with less developed technology and unreasonable energy utilization.China’s OFDI can promote technological progress in these countries and making full use of their advantage resources for economic development.As China’s economy has entered a“new normal”,its global influence has risen,and the relationship between the host countries and China has also mattered more on China’s OFDI decisions.This effort provides important supports for optimizing the location choices of Chinese enterprises’foreign investment.