Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to remyelination.Failure of remyelination due to oligodendrocyte progenitor cell death can result in severe n...Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to remyelination.Failure of remyelination due to oligodendrocyte progenitor cell death can result in severe nerve damage.Ferroptosis is an iron-dependent form of regulated cell death caused by membrane rupture induced by lipid peroxidation,and plays an important role in the pathological process of ischemic stroke.However,there are few studies on oligodendrocyte progenitor cell ferroptosis.We analyzed transcriptome sequencing data from GEO databases and identified a role of ferroptosis in oligodendrocyte progenitor cell death and myelin injury after cerebral ischemia.Bioinformatics analysis suggested that perilipin-2(PLIN2)was involved in oligodendrocyte progenitor cell ferroptosis.PLIN2 is a lipid storage protein and a marker of hypoxia-sensitive lipid droplet accumulation.For further investigation,we established a mouse model of cerebral ischemia/reperfusion.We found significant myelin damage after cerebral ischemia,as well as oligodendrocyte progenitor cell death and increased lipid peroxidation levels around the infarct area.The ferroptosis inhibitor,ferrostatin-1,rescued oligodendrocyte progenitor cell death and subsequent myelin injury.We also found increased PLIN2 levels in the peri-infarct area that co-localized with oligodendrocyte progenitor cells.Plin2 knockdown rescued demyelination and improved neurological deficits.Our findings suggest that targeting PLIN2 to regulate oligodendrocyte progenitor cell ferroptosis may be a potential therapeutic strategy for rescuing myelin damage after cerebral ischemia.展开更多
The pressure and temperature significantly influence precipitable water vapor(PWV) retrieval. Global Navigation Satellite System(GNSS) PWV retrieval is limited because the GNSS stations lack meteorological sensors. Fi...The pressure and temperature significantly influence precipitable water vapor(PWV) retrieval. Global Navigation Satellite System(GNSS) PWV retrieval is limited because the GNSS stations lack meteorological sensors. First, this article evaluated the accuracy of pressure and temperature in 68 radiosonde stations in China based on ERA5 Reanalysis data from 2015 to 2019 and compared them with GPT3model. Then, the accuracy of pressure and temperature calculated by ERA5 were estimated in 5 representative IGS stations in China. And the PWV calculated by these meteorological parameters from ERA5(ERA5-PWV) were analyzed. Finally, the relation between ERA5-PWV and precipitation was deeply explored using wavelet coherence analysis in IGS stations. These results indicate that the accuracy of pressure and temperature of ERA5 is better than the GPT3 model. In radiosonde stations, the mean BIAS and MAE of pressure and temperature in ERA5 are-0.41/1.15 hpa and-0.97/2.12 K. And the mean RMSEs are 1.35 hpa and 2.87 K, which improve 74.77% and 40.58% compared with GPT3 model. The errors of pressure and temperature of ERA5 are smaller than the GPT3 model in bjfs, hksl and wuh2, and the accuracy of ERA5-PWV is improved by 18.77% compared with the GPT3 model. In addition, there is a significant positive correlation between ERA5-PWV and precipitation. And precipitation is always associated with the sharp rise of ERA5-PWV, which provides important references for rainfall prediction.展开更多
Waste water treatment process(WWTP)control has been attracting more and more attention.However,various undesired factors,such as disturbance,uncertainties,and strong nonlinear couplings,propose big challenges to the c...Waste water treatment process(WWTP)control has been attracting more and more attention.However,various undesired factors,such as disturbance,uncertainties,and strong nonlinear couplings,propose big challenges to the control of a WWTP.In order to improve the control performance of the closed-loop system and guarantee the discharge requirements of the effluent quality,rather than take the model dependent control approaches,an active disturbance rejection control(ADRC)is utilized.Based on the control signal and system output,a phase optimized ADRC(POADRC)is designed to control the dissolved oxygen and nitrate concentration in a WWTP.The phase advantage of the phase optimized extended state observer(POESO),convergence of the POESO,and stability of the closed-loop system are analyzed from the theoretical point of view.Finally,a commonly accepted benchmark simulation model no.1.(BSM1)is utilized to test the POESO and POADRC.Linear active disturbance rejection control(LADRC)and the suggested proportion-integration(PI)control are taken to make a comparative research.Both system responses and performance index values confirm the advantage of the POADRC over the LADRC and the suggested PI control.Numerical results show that,as a result of the leading phase of the total disturbance estimation,the POESO based POADRC is an effective and promising way to control the dissolved oxygen and nitrate concentration so as to ensure the effluent quality of a WWTP.展开更多
A pioneering glass-compatible transparent temperature alarm system self-powered by luminescent solar concentrators(LSCs) is reported.Single green-emitted organic manganese halides(OMHs) of PEA_(2)MnBr_(2)I_(2),which h...A pioneering glass-compatible transparent temperature alarm system self-powered by luminescent solar concentrators(LSCs) is reported.Single green-emitted organic manganese halides(OMHs) of PEA_(2)MnBr_(2)I_(2),which has a unique temperature-dependent backward energy transfer process from selftrapped state to^(4)T_(1)energy level of Mn,is used for triggering the temperature alarm.The LSC with redemitted CsPbI_(3)perovskite-polymer composite films on the glass substrate is used for power supply.The spectrally separated nature between the green-emitted OMHs for temperature alarm and red-emitted CsPbI3in LSC for power supply allows for probing the signal light of temperature-responsive OMHs without the interference of LSCs,making it possible to calibrate the temperature visually just by a self-powered brightness detection circuit with LED indicators.Taking advantage of LSC without hot spot effects plaguing the solar cells,as-prepared temperature alarm system can operate well on both sunny and cloudy day.展开更多
[Objectives]This study was conducted to investigate the quality standard of Heracleum moellendorffii Hance.,so as to provide data support for the 2003 version of Quality Standards for Chinese Medicinal Materials and E...[Objectives]This study was conducted to investigate the quality standard of Heracleum moellendorffii Hance.,so as to provide data support for the 2003 version of Quality Standards for Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province.[Methods]H.moellendorffii was subjected to character identification and microscopic identification.Referring to Chinese Pharmacopoeia,TCL thin-layer identification and extract determination were carried out on 10 batches of H.moellendorffii from 6 habitats in Guizhou.[Results]The characters and microscopic characteristics of H.moellendorffii were described in detail.The TLC thin-layer identification spots were clear,with strong specificity.The limit of the medicinal material extract was determined to be not less than 22.0%.[Conclusions]This study can provide data support for the quality evaluation and standard improvement of the medicinal material H.moellendorffii.展开更多
[Objectives]This study was conducted to analyze the Raphanus sativus L.medicinal materials from different origins and batches in Guizhou Province to explore its quality standards.[Methods]The plant morphology,material...[Objectives]This study was conducted to analyze the Raphanus sativus L.medicinal materials from different origins and batches in Guizhou Province to explore its quality standards.[Methods]The plant morphology,material shape and microscopic characteristics of R.sativus were identified;and qualitative identification was performed by the TLC method.[Results]The plant morphology,material shape and microscopic characteristics of the R.sativus medicinal materials were described;and the TLC identification method of the R.sativus medicinal materials was established,with clear spots,good separation,and good specificity.[Conclusions]The method is accurate,simple,highly sensitive,interference-free,has good reproducibility,and can be used as a data support for the formulation of quality standards for R.sativus medicinal materials.展开更多
[Objectives]This study was conducted to establish a method for determining the contents of Chaenomeles cathayensis(Hemsl.)Schneid.,and analyze the changes in effective components of C.cathayensis grown in Guizhou,so a...[Objectives]This study was conducted to establish a method for determining the contents of Chaenomeles cathayensis(Hemsl.)Schneid.,and analyze the changes in effective components of C.cathayensis grown in Guizhou,so as to provide data support for the production and quality control of C.cathayensis.[Methods]The contents of ursolic acid and oleanolic acid in C.cathayensis in different areas of Guizhou were determined.The HPLC method was adopted under following conditions:chromatographic column waters C18 column(4.6 mm×150 mm,5.0μm);mobile phase:methanol-0.1 mol/L ammonium acetate(85∶15);column temperature:25℃;detection wavelength:257 nm;flow rate 1.0 ml/min.[Results]Through methodological investigations,HPLC could be used to detect the contents of the two terpenoids oleanolic acid and ursolic acid in C.cathayensis from Guizhou.The content of oleanolic acid ranged from 0.076%to 0.144%,and the content of ursolic acid ranged from 0.201%to 0.439%.[Conclusions]A method for determining the contents of C.cathayensis was established using the HPLC method with oleanolic acid and ursolic acid as the index components.The method is accurate,reliable,and simple and easy to implement,and can serve as a reference and support for quality evaluation and standard improvement of C.cathayensis.展开更多
[Objectives]Characters and microscopic characteristics of Aralia cordata were studied,to improve identification method for Aralia cordata.[Methods]Using pharmacognostic identification method,original plant morphology ...[Objectives]Characters and microscopic characteristics of Aralia cordata were studied,to improve identification method for Aralia cordata.[Methods]Using pharmacognostic identification method,original plant morphology of Aralia cordata was observed,and microscopic observation of its root cross section and powder was conducted.[Results]It is perennial herb,with rhizome recess,arranged in clusters of nodules.There are double or triple pinnate compound leaves.It is glabrous or sparsely pubescent,with umbel.Flowers are white,while fruits are spherical and purple black.In microstructure,the cork layer on the cross section of root consists of 6-10 rows of cells,and there is secondary cortex.The parenchyma cells are rich in starch granules,and the cambium is not obvious.The vessels are uniserial,and the wood fiber is less,and the primary xylem is bipartite.There are many starch granules in powder,mostly single granules,round or quasi round,oval,umbilicus herringbone or short slit,with many marginal pits and reticulate ducts.Calcium oxalate cluster crystals are mostly gathered.Cork cells are yellowish brown and arranged neatly.The fibrous wall is thin,and the pore groove is obvious,and stone cells are occasionally seen.[Conclusions]Using character and microscopic identification method,it could accurately identify medicinal material of Aralia cordata,and provide reference basis for development and utilization of its resources.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82071307(to HL),82271362(to HL),82171294(to JW),82371303(to JW),and 82301460(to PX)the Natural Science Foundation of Jiangsu Province,No.BK20211552(to HL)+1 种基金Suzhou Medical Technology Innovation Project-Clinical Frontier,No.SKY2022002(to ZY)the Science and Education Foundation for Health of Suzhou for Youth,No.KJXW2023001(to XL)。
文摘Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to remyelination.Failure of remyelination due to oligodendrocyte progenitor cell death can result in severe nerve damage.Ferroptosis is an iron-dependent form of regulated cell death caused by membrane rupture induced by lipid peroxidation,and plays an important role in the pathological process of ischemic stroke.However,there are few studies on oligodendrocyte progenitor cell ferroptosis.We analyzed transcriptome sequencing data from GEO databases and identified a role of ferroptosis in oligodendrocyte progenitor cell death and myelin injury after cerebral ischemia.Bioinformatics analysis suggested that perilipin-2(PLIN2)was involved in oligodendrocyte progenitor cell ferroptosis.PLIN2 is a lipid storage protein and a marker of hypoxia-sensitive lipid droplet accumulation.For further investigation,we established a mouse model of cerebral ischemia/reperfusion.We found significant myelin damage after cerebral ischemia,as well as oligodendrocyte progenitor cell death and increased lipid peroxidation levels around the infarct area.The ferroptosis inhibitor,ferrostatin-1,rescued oligodendrocyte progenitor cell death and subsequent myelin injury.We also found increased PLIN2 levels in the peri-infarct area that co-localized with oligodendrocyte progenitor cells.Plin2 knockdown rescued demyelination and improved neurological deficits.Our findings suggest that targeting PLIN2 to regulate oligodendrocyte progenitor cell ferroptosis may be a potential therapeutic strategy for rescuing myelin damage after cerebral ischemia.
基金supported by Open Fund of Key Laboratory of Mine Environmental Monitoring and Improving around Poyang Lake of Ministry of Natural Resources (Grant MEMI-2021-2022-27)funded by the National Natural Science Foundation of China (Grants 41904031,42374040,42061077)+2 种基金the Jiangxi Provincial Natural Science Foundation (Grants 20202BABL213033)the State Key Laboratory of Geodesy and Earth's Dynamics (Grants SKLGED2021-2-2)the Graduate Innovation Foundation of East China University of Technology (Grants YC2022-s604,YC2022-s609)。
文摘The pressure and temperature significantly influence precipitable water vapor(PWV) retrieval. Global Navigation Satellite System(GNSS) PWV retrieval is limited because the GNSS stations lack meteorological sensors. First, this article evaluated the accuracy of pressure and temperature in 68 radiosonde stations in China based on ERA5 Reanalysis data from 2015 to 2019 and compared them with GPT3model. Then, the accuracy of pressure and temperature calculated by ERA5 were estimated in 5 representative IGS stations in China. And the PWV calculated by these meteorological parameters from ERA5(ERA5-PWV) were analyzed. Finally, the relation between ERA5-PWV and precipitation was deeply explored using wavelet coherence analysis in IGS stations. These results indicate that the accuracy of pressure and temperature of ERA5 is better than the GPT3 model. In radiosonde stations, the mean BIAS and MAE of pressure and temperature in ERA5 are-0.41/1.15 hpa and-0.97/2.12 K. And the mean RMSEs are 1.35 hpa and 2.87 K, which improve 74.77% and 40.58% compared with GPT3 model. The errors of pressure and temperature of ERA5 are smaller than the GPT3 model in bjfs, hksl and wuh2, and the accuracy of ERA5-PWV is improved by 18.77% compared with the GPT3 model. In addition, there is a significant positive correlation between ERA5-PWV and precipitation. And precipitation is always associated with the sharp rise of ERA5-PWV, which provides important references for rainfall prediction.
基金supported by the Key program of Beijing Municipal Education Commission(KZ201810011012)National Natural Science Foundation of China(61873005)Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 13th Fiveyear Plan(CIT&TCD201704044)。
文摘Waste water treatment process(WWTP)control has been attracting more and more attention.However,various undesired factors,such as disturbance,uncertainties,and strong nonlinear couplings,propose big challenges to the control of a WWTP.In order to improve the control performance of the closed-loop system and guarantee the discharge requirements of the effluent quality,rather than take the model dependent control approaches,an active disturbance rejection control(ADRC)is utilized.Based on the control signal and system output,a phase optimized ADRC(POADRC)is designed to control the dissolved oxygen and nitrate concentration in a WWTP.The phase advantage of the phase optimized extended state observer(POESO),convergence of the POESO,and stability of the closed-loop system are analyzed from the theoretical point of view.Finally,a commonly accepted benchmark simulation model no.1.(BSM1)is utilized to test the POESO and POADRC.Linear active disturbance rejection control(LADRC)and the suggested proportion-integration(PI)control are taken to make a comparative research.Both system responses and performance index values confirm the advantage of the POADRC over the LADRC and the suggested PI control.Numerical results show that,as a result of the leading phase of the total disturbance estimation,the POESO based POADRC is an effective and promising way to control the dissolved oxygen and nitrate concentration so as to ensure the effluent quality of a WWTP.
基金supported by the Natural Science Foundation of China(22075043,21875034,61704093)。
文摘A pioneering glass-compatible transparent temperature alarm system self-powered by luminescent solar concentrators(LSCs) is reported.Single green-emitted organic manganese halides(OMHs) of PEA_(2)MnBr_(2)I_(2),which has a unique temperature-dependent backward energy transfer process from selftrapped state to^(4)T_(1)energy level of Mn,is used for triggering the temperature alarm.The LSC with redemitted CsPbI_(3)perovskite-polymer composite films on the glass substrate is used for power supply.The spectrally separated nature between the green-emitted OMHs for temperature alarm and red-emitted CsPbI3in LSC for power supply allows for probing the signal light of temperature-responsive OMHs without the interference of LSCs,making it possible to calibrate the temperature visually just by a self-powered brightness detection circuit with LED indicators.Taking advantage of LSC without hot spot effects plaguing the solar cells,as-prepared temperature alarm system can operate well on both sunny and cloudy day.
基金Science and Technology Innovation Platform Construction Project of Anshun City(ASKP[2017]03).
文摘[Objectives]This study was conducted to investigate the quality standard of Heracleum moellendorffii Hance.,so as to provide data support for the 2003 version of Quality Standards for Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province.[Methods]H.moellendorffii was subjected to character identification and microscopic identification.Referring to Chinese Pharmacopoeia,TCL thin-layer identification and extract determination were carried out on 10 batches of H.moellendorffii from 6 habitats in Guizhou.[Results]The characters and microscopic characteristics of H.moellendorffii were described in detail.The TLC thin-layer identification spots were clear,with strong specificity.The limit of the medicinal material extract was determined to be not less than 22.0%.[Conclusions]This study can provide data support for the quality evaluation and standard improvement of the medicinal material H.moellendorffii.
基金Science and Technology Innovation Platform Construction Project of Anshun City(ASKP[2017]03)Anshun Traditional Chinese Medicine(Ethnic Medicine)Inspection Technology Innovation Talent Team(ASKC[2018]09).
文摘[Objectives]This study was conducted to analyze the Raphanus sativus L.medicinal materials from different origins and batches in Guizhou Province to explore its quality standards.[Methods]The plant morphology,material shape and microscopic characteristics of R.sativus were identified;and qualitative identification was performed by the TLC method.[Results]The plant morphology,material shape and microscopic characteristics of the R.sativus medicinal materials were described;and the TLC identification method of the R.sativus medicinal materials was established,with clear spots,good separation,and good specificity.[Conclusions]The method is accurate,simple,highly sensitive,interference-free,has good reproducibility,and can be used as a data support for the formulation of quality standards for R.sativus medicinal materials.
基金Science and Technology Innovation Platform Construction Project of Anshun City(ASKP[2017]03)。
文摘[Objectives]This study was conducted to establish a method for determining the contents of Chaenomeles cathayensis(Hemsl.)Schneid.,and analyze the changes in effective components of C.cathayensis grown in Guizhou,so as to provide data support for the production and quality control of C.cathayensis.[Methods]The contents of ursolic acid and oleanolic acid in C.cathayensis in different areas of Guizhou were determined.The HPLC method was adopted under following conditions:chromatographic column waters C18 column(4.6 mm×150 mm,5.0μm);mobile phase:methanol-0.1 mol/L ammonium acetate(85∶15);column temperature:25℃;detection wavelength:257 nm;flow rate 1.0 ml/min.[Results]Through methodological investigations,HPLC could be used to detect the contents of the two terpenoids oleanolic acid and ursolic acid in C.cathayensis from Guizhou.The content of oleanolic acid ranged from 0.076%to 0.144%,and the content of ursolic acid ranged from 0.201%to 0.439%.[Conclusions]A method for determining the contents of C.cathayensis was established using the HPLC method with oleanolic acid and ursolic acid as the index components.The method is accurate,reliable,and simple and easy to implement,and can serve as a reference and support for quality evaluation and standard improvement of C.cathayensis.
基金Supported by Anshun Science and Technology Innovation Platform Construction Plan Project(ANSHIKEPING[2017]03)。
文摘[Objectives]Characters and microscopic characteristics of Aralia cordata were studied,to improve identification method for Aralia cordata.[Methods]Using pharmacognostic identification method,original plant morphology of Aralia cordata was observed,and microscopic observation of its root cross section and powder was conducted.[Results]It is perennial herb,with rhizome recess,arranged in clusters of nodules.There are double or triple pinnate compound leaves.It is glabrous or sparsely pubescent,with umbel.Flowers are white,while fruits are spherical and purple black.In microstructure,the cork layer on the cross section of root consists of 6-10 rows of cells,and there is secondary cortex.The parenchyma cells are rich in starch granules,and the cambium is not obvious.The vessels are uniserial,and the wood fiber is less,and the primary xylem is bipartite.There are many starch granules in powder,mostly single granules,round or quasi round,oval,umbilicus herringbone or short slit,with many marginal pits and reticulate ducts.Calcium oxalate cluster crystals are mostly gathered.Cork cells are yellowish brown and arranged neatly.The fibrous wall is thin,and the pore groove is obvious,and stone cells are occasionally seen.[Conclusions]Using character and microscopic identification method,it could accurately identify medicinal material of Aralia cordata,and provide reference basis for development and utilization of its resources.
