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Effective Method to Resolve the Chromosome Numbers in Pistacia Species (Anacardiaceae)
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作者 mohannad al-saghir Shadi Abu Baker Robert Pusok 《American Journal of Plant Sciences》 2014年第20期2913-2916,共4页
The genus Pistacia L. belongs to the Anacardiaceae family and includes at least eleven species. Cytogenetic studies addressing the genus Pistacia are rather few. Chromosome numbers of the different Pistacia species, r... The genus Pistacia L. belongs to the Anacardiaceae family and includes at least eleven species. Cytogenetic studies addressing the genus Pistacia are rather few. Chromosome numbers of the different Pistacia species, revealed by these studies, are questionable due to the fact that poor chromosome counting protocols were used, and these protocols are hampered by the extremely small-sized chromosomes of Pistacia species. The aim of this study was to develop a more effective method to resolve the chromosome numbers in Pistacia species using a fluorescent microscope. The method described here is modified from the Sigma Plant Protoplast Digest/Wash Solution protocol. The method used here is highly effective for karyotyping analysis and studying population genetics of Pistacia species. Moreover, it is easy and can be reproduced for other species that have smaller chromosomes. This method can be used for plant herbarium specimens or field plants. This study provides valuable chromosomal data for cytogeneticists and plant breeders who are working on this genus. It provides additional insight into understanding the taxonomic and phylogenetic relationships among Pistacia species. The chromosomes described here are also suitable for gene and genome mapping. 展开更多
关键词 PISTACIA CYTOGENETICS CHROMOSOME NUMBERS Fluorescent Microscopy
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Review of general, organic, and biological chemistry, second edition
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作者 Shadi Abu-Baker Shahrokh Ghaffari +1 位作者 mohannad al-saghir Parinbam K. Thamburaj 《Natural Science》 2014年第1期14-16,共3页
Overall, General, Organic, and Biological Chemistry is a very useful text to support a twosemester undergraduate course series in chemistry for health sciences students. Its strengths are the use of the P.A.V.E. way t... Overall, General, Organic, and Biological Chemistry is a very useful text to support a twosemester undergraduate course series in chemistry for health sciences students. Its strengths are the use of the P.A.V.E. way to student learning applications. It is very obvious that the author has the background and the preparation of the student taking the course(s) in mind when she was writing the textbook. 展开更多
关键词 TEXT BOOK Review Education CHEMISTRY BIOCHEMISTRY ORGANIC
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Microwave Assisted Peptide Synthesis as a New Gold Standard in Solid Phase Peptide Synthesis: Phospholamban as an Example
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作者 Shadi Abu-Baker Philip Garber +4 位作者 Bryce Hina Trevor Reed Ghaffari Shahrokh mohannad al-saghir Gary Lorigan 《Open Journal of Synthesis Theory and Applications》 2014年第1期1-4,共4页
In this study, we report the microwave assisted synthesis of Phospholamban protein (WT-PLB) as a new gold standard in solid phase peptide synthesis. Microwave energy offers benefits for both the coupling and deprotect... In this study, we report the microwave assisted synthesis of Phospholamban protein (WT-PLB) as a new gold standard in solid phase peptide synthesis. Microwave energy offers benefits for both the coupling and deprotection reactions during peptide synthesis. The use of microwave energy for both the coupling and deprotection steps makes the microwave peptide synthesizers the most versatile and powerful systems available. It produces high yield and fast synthesis when compared to conventional peptide synthesizers. 展开更多
关键词 SOLID-STATE PEPTIDE SYNTHESIS PHOSPHOLAMBAN Microwave Assisted SYNTHESIS
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