Objectives:Non-small cell lung cancer(NSCLC)represents a formidable malignancy characterized by its marked metastatic potential and intrinsic resistance to therapeutic interventions.The identification of potential bio...Objectives:Non-small cell lung cancer(NSCLC)represents a formidable malignancy characterized by its marked metastatic potential and intrinsic resistance to therapeutic interventions.The identification of potential biomarkers delineating the progression and metastatic cascade of NSCLC assumes paramount importance in fostering advancements toward enhanced patient outcomes and prognostic stratification.Methods:The expression level of the actin-related protein 2/3 complex;subunit 1A(ARPC1A)in NSCLC was evaluated using The Cancer Genome Atlas(TCGA)and Gene Expression Profiling Interactive Analysis(GEPIA)databases;along with the LinkedOmics database for co-expression genes.Further verification of ARPC1A expression in normal lung cells and NSCLC cells;as well as in normal tissues and lung cancer tissues;was performed using quantitative real-time reverse transcription PCR(RTqPCR)and Western blotting.The function of ARPC1A was explored through Gene Set Enrichment Analysis(GSEA)and immune infiltration analysis;followed by functional experiments for validation.Results:ARPC1A is upregulated in NSCLC and is associated with unfavorable clinical prognoses.Additionally,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis highlights a potential link between the ARPC1A gene and the cell cycle and p53 signaling pathways.ARPC1A also promotes cell proliferation and resistance to chemotherapeutic drugs,thereby enhancing the oncogenic potential of NSCLC.Relevant cell-based experiments confirm that targeted inhibition of ARPC1A effectively suppresses cellular migratory and invasive capabilities.The immune infiltration analysis showed a close association between ARPC1A expression and various immune components,suggesting ARPC1A may interact with the tumor microenvironment.Mechanistically,ARPC1A promotes cell migration by stimulating the epithelialto-mesenchymal transition(EMT).Conclusion:The study results revealed the potential of ARPC1A as a valuable prognostic biomarker for NSCLC.Additionally,the associated mechanisms provide insights that may pave the way for therapeutic interventions for NSCLC patients.展开更多
A spray-drying assisted solid-state method to prepare spherical layer-structured H_(2)TiO_(3) ion sieve(LSTIS)particles is reported herein.The effects of synthesis parameters(calcination temperature,calcination time,a...A spray-drying assisted solid-state method to prepare spherical layer-structured H_(2)TiO_(3) ion sieve(LSTIS)particles is reported herein.The effects of synthesis parameters(calcination temperature,calcination time,and the lithium-titanium molar ratio)on adsorption-desorption performance(the delithiation ratio,titanium dissolution loss,and the adsorption capacity)were investigated.The as-prepared LSTIS exhibited an equilibrium adsorption capacity of 30.08 mg·g^(-1)(average of 25.85 mg·g^(-1) over 5 cycles)and ultra-low titanium dissolution loss of less than 0.12%(average of 0.086%over 5 cycles).The LSTIS showed excellent selectivity toward Li^(+) in Na^(+),K^(+),Mg^(2+),and Ca^(2+) coexisting saline solutions where its adsorption capacity reached 27.45 mg·g^(-1) and the separation factors of Li^(+) over the coexisting cations exceeded 100.The data suggests that the LSTIS is promising to competitively enrich Li^(+) from saline solutions.展开更多
Emerging cohorts and basic studies have associated certain genetic modifications in cancer patients,such as gene mutation,amplification,or deletion,with the overall survival prognosis,underscoring patients’genetic ba...Emerging cohorts and basic studies have associated certain genetic modifications in cancer patients,such as gene mutation,amplification,or deletion,with the overall survival prognosis,underscoring patients’genetic background may directly regulate drug sensitivity/resistance during chemotherapies.Understanding the molecular mechanism underpinning drug sensitivity/resistance and further uncovering the effective drugs have been the major ambition in the cancer drug discovery.The emergence and popularity of CRISPR/Cas9 technology have reformed the entire life science research,providing a precise and simplified genome editing tool with unlimited editing possibilities.Furthermore,it presents a powerful tool in cancer drug discovery,which hopefully facilitates us with a rapid and reliable manner in developing novel therapies and understanding the molecular mechanisms of drug sensitivity/resistance.Herein,we summarized the application of CRISPR/Cas9 in drug screening,with the focus on CRISPR/Cas9 mediated gene knockout,gene knock-in,as well as transcriptional modification.Additionally,this review provides the concerns,cautions,and ethnic considerations that need to be taken when applying CRISPR in the drug discovery.展开更多
PAHs are one of the environmental contaminants of greatest concern on global scales due to their ecological risk.This study collected the polycyclic aromatic hydrocarbon(PAH)concentration data of six different media i...PAHs are one of the environmental contaminants of greatest concern on global scales due to their ecological risk.This study collected the polycyclic aromatic hydrocarbon(PAH)concentration data of six different media in Kenya and evaluated their ecological risk assessment.Twenty-five literatures related to PAHs pollution in Kenya were found to conduct a meta-analysis.Our meta-analysis shows that the geometric means of PAH pollution in water(481.49 ng L^(-1)),sediment(519.15 ng g^(-1))t,soil(337.46 ng g^(-1)),air(38.60 ng m^(-3)),food(33.12 ng g^(-1)),and other(111,176.90 ng g^(-1))six media in Kenya had different degrees.The primary sources were combustion sources.A small number of PAHs are petroleum sources.A variety of methods were used to assess the ecological risk of PAHs in different media in Kenya,and we used a tiered approach to assess the ecological risk of water.The risk assessment result showed that the high contamination of Acenaphthylene and Acenaphthelene found in water in Kenya may pose certain ecological risks,which requires necessary measures.The concentration of Benzo[k]fluoranthene in sediment and the concentration of carcinogenic PAHs in soil can also cause the ecological risks,which should be paid more attention.In light of the PAH contamination in various media found in Kenya in the study,further detailed studies should be carried out to provide a more detailed analysis of the distribution of PAHs in Kenya to prevent the severe impact of PAHs contamination in Kenya and Africa.展开更多
基金supported by the Natural Science Foundation of Anhui Province(ML 2308085MC80)the Anhui Medical University Research and Innovation Talent Team(KZ).
文摘Objectives:Non-small cell lung cancer(NSCLC)represents a formidable malignancy characterized by its marked metastatic potential and intrinsic resistance to therapeutic interventions.The identification of potential biomarkers delineating the progression and metastatic cascade of NSCLC assumes paramount importance in fostering advancements toward enhanced patient outcomes and prognostic stratification.Methods:The expression level of the actin-related protein 2/3 complex;subunit 1A(ARPC1A)in NSCLC was evaluated using The Cancer Genome Atlas(TCGA)and Gene Expression Profiling Interactive Analysis(GEPIA)databases;along with the LinkedOmics database for co-expression genes.Further verification of ARPC1A expression in normal lung cells and NSCLC cells;as well as in normal tissues and lung cancer tissues;was performed using quantitative real-time reverse transcription PCR(RTqPCR)and Western blotting.The function of ARPC1A was explored through Gene Set Enrichment Analysis(GSEA)and immune infiltration analysis;followed by functional experiments for validation.Results:ARPC1A is upregulated in NSCLC and is associated with unfavorable clinical prognoses.Additionally,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis highlights a potential link between the ARPC1A gene and the cell cycle and p53 signaling pathways.ARPC1A also promotes cell proliferation and resistance to chemotherapeutic drugs,thereby enhancing the oncogenic potential of NSCLC.Relevant cell-based experiments confirm that targeted inhibition of ARPC1A effectively suppresses cellular migratory and invasive capabilities.The immune infiltration analysis showed a close association between ARPC1A expression and various immune components,suggesting ARPC1A may interact with the tumor microenvironment.Mechanistically,ARPC1A promotes cell migration by stimulating the epithelialto-mesenchymal transition(EMT).Conclusion:The study results revealed the potential of ARPC1A as a valuable prognostic biomarker for NSCLC.Additionally,the associated mechanisms provide insights that may pave the way for therapeutic interventions for NSCLC patients.
基金financially supported by the Prospective Joint Research Project of Industry,University and Research in Jiangsu Province(BY2016005-11)National Science and Technology Support Plan(No.2013BAE111B03)。
文摘A spray-drying assisted solid-state method to prepare spherical layer-structured H_(2)TiO_(3) ion sieve(LSTIS)particles is reported herein.The effects of synthesis parameters(calcination temperature,calcination time,and the lithium-titanium molar ratio)on adsorption-desorption performance(the delithiation ratio,titanium dissolution loss,and the adsorption capacity)were investigated.The as-prepared LSTIS exhibited an equilibrium adsorption capacity of 30.08 mg·g^(-1)(average of 25.85 mg·g^(-1) over 5 cycles)and ultra-low titanium dissolution loss of less than 0.12%(average of 0.086%over 5 cycles).The LSTIS showed excellent selectivity toward Li^(+) in Na^(+),K^(+),Mg^(2+),and Ca^(2+) coexisting saline solutions where its adsorption capacity reached 27.45 mg·g^(-1) and the separation factors of Li^(+) over the coexisting cations exceeded 100.The data suggests that the LSTIS is promising to competitively enrich Li^(+) from saline solutions.
基金This work was supported by the Start-Up funding from Anhui Medical University(KZ 0801033201),and the Doctoral Research Funding from the Cancer Society of Finland(KZ).
文摘Emerging cohorts and basic studies have associated certain genetic modifications in cancer patients,such as gene mutation,amplification,or deletion,with the overall survival prognosis,underscoring patients’genetic background may directly regulate drug sensitivity/resistance during chemotherapies.Understanding the molecular mechanism underpinning drug sensitivity/resistance and further uncovering the effective drugs have been the major ambition in the cancer drug discovery.The emergence and popularity of CRISPR/Cas9 technology have reformed the entire life science research,providing a precise and simplified genome editing tool with unlimited editing possibilities.Furthermore,it presents a powerful tool in cancer drug discovery,which hopefully facilitates us with a rapid and reliable manner in developing novel therapies and understanding the molecular mechanisms of drug sensitivity/resistance.Herein,we summarized the application of CRISPR/Cas9 in drug screening,with the focus on CRISPR/Cas9 mediated gene knockout,gene knock-in,as well as transcriptional modification.Additionally,this review provides the concerns,cautions,and ethnic considerations that need to be taken when applying CRISPR in the drug discovery.
基金Opening Funding Project of Key Laboratory of Aquatic Botany and Watershed Ecology,Chinese Academy of Sciences(No.E0520202)and ProjectSAJC202102 supported by SAJOREC,CAS。
文摘PAHs are one of the environmental contaminants of greatest concern on global scales due to their ecological risk.This study collected the polycyclic aromatic hydrocarbon(PAH)concentration data of six different media in Kenya and evaluated their ecological risk assessment.Twenty-five literatures related to PAHs pollution in Kenya were found to conduct a meta-analysis.Our meta-analysis shows that the geometric means of PAH pollution in water(481.49 ng L^(-1)),sediment(519.15 ng g^(-1))t,soil(337.46 ng g^(-1)),air(38.60 ng m^(-3)),food(33.12 ng g^(-1)),and other(111,176.90 ng g^(-1))six media in Kenya had different degrees.The primary sources were combustion sources.A small number of PAHs are petroleum sources.A variety of methods were used to assess the ecological risk of PAHs in different media in Kenya,and we used a tiered approach to assess the ecological risk of water.The risk assessment result showed that the high contamination of Acenaphthylene and Acenaphthelene found in water in Kenya may pose certain ecological risks,which requires necessary measures.The concentration of Benzo[k]fluoranthene in sediment and the concentration of carcinogenic PAHs in soil can also cause the ecological risks,which should be paid more attention.In light of the PAH contamination in various media found in Kenya in the study,further detailed studies should be carried out to provide a more detailed analysis of the distribution of PAHs in Kenya to prevent the severe impact of PAHs contamination in Kenya and Africa.
