The process of the gas jet from aircraft engines impacting a jet blast deflector is not only a complex fluid–solid coupling problem that is not easy to compute, but also a safety issue that seriously interferes with ...The process of the gas jet from aircraft engines impacting a jet blast deflector is not only a complex fluid–solid coupling problem that is not easy to compute, but also a safety issue that seriously interferes with flight deck envi?ronment. The computational fluid dynamics(CFD) method is used to simulate numerically the impact e ect of gas jet from aircraft engines on a jet blast deflector by using the Reynolds?averaged Navier?Stokes(RANS) equations and turbulence models. First of all, during the pre?processing of numerical computation, a sub?domains hybrid meshing scheme is adopted to reduce mesh number and improve mesh quality. Then, four di erent turbulence models includ?ing shear?stress transport(SST) k-w, standard k-w, standard k-ε and Reynolds stress model(RSM) are used to compare and verify the correctness of numerical methods for gas jet from a single aircraft engine. The predicted values are in good agreement with the experimental data, and the distribution and regularity of shock wave, velocity, pressure and temperature of a single aircraft engine are got. The results show that SST k?w turbulence model is more suitable for the numerical simulation of compressible viscous gas jet with high prediction accuracy. Finally, the impact e ect of gas jet from two aircraft engines on a jet blast deflector is analyzed based on the above numerical method, not only the flow parameters of gas jet and the interaction regularity between gas jet and the jet blast deflector are got, but also the thermal shock properties and dynamic impact characteristics of gas jet impacting the jet blast deflector are got. So the dangerous activity area of crew and equipments on the flight deck can be predicted qualitatively and quantitatively. The proposed research explores out a correct numerical method for the fluid–solid interaction during the impact process of supersonic gas jet, which provides an e ective technical support for design, thermal ablation and structural damage analysis of a new jet blast deflector.展开更多
Objective To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B＇/C virus-infected ART-naive individuals. Methods HIV-1-specific CTL responses were analyzed by IFN-7 ELISPOT assay us...Objective To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B＇/C virus-infected ART-naive individuals. Methods HIV-1-specific CTL responses were analyzed by IFN-7 ELISPOT assay using overlapping peptides spanning the consensus sequences of HIV-1 clade C Tat and Rev proteins. Statistical analysis and graphical presentation were performed using SIGMAPLOT 10.0 and SIGMASTAT 3.5. For samples with a positive response, the magnitude of CTL responses was compared between HIV-1 C proteins by Wilcoxon rank sum test, and the significance threshold was P〈0.05. Results Tat and Rev were frequently recognized, with 23% and 52% of the tested individuals having detectable responses to these proteins, respectively. Several immunodominant regions were detected in Rev. No significant correlation was observed between the magnitude and breadth of CTL responses to regulatory proteins and the control of virus replication in this study. Conclusion Tat and Rev can serve as targets for HIV-l-specific CTL, and several immunodominant regions are detectable in Rev. Further characterization of epitopes and their role in virus control may shed light on pathogenesis of HIV- 1 natural infection and also be useful for the design and testing of candidate vaccines.展开更多
基金Supported by National Natural Science Foundation of China(Grant No.51505491)Shandong Provincial Natural Science Foundation of China(Grant No.ZR2014EEP019)
文摘The process of the gas jet from aircraft engines impacting a jet blast deflector is not only a complex fluid–solid coupling problem that is not easy to compute, but also a safety issue that seriously interferes with flight deck envi?ronment. The computational fluid dynamics(CFD) method is used to simulate numerically the impact e ect of gas jet from aircraft engines on a jet blast deflector by using the Reynolds?averaged Navier?Stokes(RANS) equations and turbulence models. First of all, during the pre?processing of numerical computation, a sub?domains hybrid meshing scheme is adopted to reduce mesh number and improve mesh quality. Then, four di erent turbulence models includ?ing shear?stress transport(SST) k-w, standard k-w, standard k-ε and Reynolds stress model(RSM) are used to compare and verify the correctness of numerical methods for gas jet from a single aircraft engine. The predicted values are in good agreement with the experimental data, and the distribution and regularity of shock wave, velocity, pressure and temperature of a single aircraft engine are got. The results show that SST k?w turbulence model is more suitable for the numerical simulation of compressible viscous gas jet with high prediction accuracy. Finally, the impact e ect of gas jet from two aircraft engines on a jet blast deflector is analyzed based on the above numerical method, not only the flow parameters of gas jet and the interaction regularity between gas jet and the jet blast deflector are got, but also the thermal shock properties and dynamic impact characteristics of gas jet impacting the jet blast deflector are got. So the dangerous activity area of crew and equipments on the flight deck can be predicted qualitatively and quantitatively. The proposed research explores out a correct numerical method for the fluid–solid interaction during the impact process of supersonic gas jet, which provides an e ective technical support for design, thermal ablation and structural damage analysis of a new jet blast deflector.
基金Ministry of Science and Technology of China (No. 2004BA719A01, 2006 CB 504207)
文摘Objective To characterize HIV-1 specific CTL responses to regulatory proteins Tat and Rev in HIV-B＇/C virus-infected ART-naive individuals. Methods HIV-1-specific CTL responses were analyzed by IFN-7 ELISPOT assay using overlapping peptides spanning the consensus sequences of HIV-1 clade C Tat and Rev proteins. Statistical analysis and graphical presentation were performed using SIGMAPLOT 10.0 and SIGMASTAT 3.5. For samples with a positive response, the magnitude of CTL responses was compared between HIV-1 C proteins by Wilcoxon rank sum test, and the significance threshold was P〈0.05. Results Tat and Rev were frequently recognized, with 23% and 52% of the tested individuals having detectable responses to these proteins, respectively. Several immunodominant regions were detected in Rev. No significant correlation was observed between the magnitude and breadth of CTL responses to regulatory proteins and the control of virus replication in this study. Conclusion Tat and Rev can serve as targets for HIV-l-specific CTL, and several immunodominant regions are detectable in Rev. Further characterization of epitopes and their role in virus control may shed light on pathogenesis of HIV- 1 natural infection and also be useful for the design and testing of candidate vaccines.
