Dwindling energy sources and a worsening environment are huge global problems,and biomass wastes are an under-exploited source of material for both energy and material generation.Herein,self-template decoction dregs o...Dwindling energy sources and a worsening environment are huge global problems,and biomass wastes are an under-exploited source of material for both energy and material generation.Herein,self-template decoction dregs of Ganoderma lucidum-derived porous carbon nanotubes(ST-DDLGCs)were synthesized via a facile and scalable strategy in response to these challenges.ST-DDLGCs exhibited a large surface area(1731.51 m^(2)g^(-1))and high pore volume(0.76 cm^(3)g^(-1)),due to the interlacing tubular structures of precursors and extra-hierarchical porous structures on tube walls.In the ST-DDLGC/PMS system,the degradation efficiency of capecitabine(CAP)reached~97.3%within 120 min.Moreover,ST-DDLGCs displayed high catalytic activity over a wide pH range of 3–9,and strong anti-interference to these typical and ubiquitous anions in wastewater and natural water bodies(i.e.,H_(2)PO_(4)^(-),NO_(3)^(-),Cl^(-) and HCO_(3)^(-)),in which a ^(1)O_(2)-dominated oxidation was identified and non-radical mechanisms were deduced.Additionally,ST-DDLGC-based coin-type symmetrical supercapacitors exhibited outstanding electrochemical performance,with specific capacitances of up to 328.1 F g^(-1)at 0.5 A g^(-1),and cycling stability of up to 98.6%after 10,000 cycles at a current density of 2 A g^(-1).The superior properties of ST-DDLGCs could be attributed to the unique porous tubular structure,which facilitated mass transfer and presented numerous active sites.The results highlight ST-DDLGCs as a potential candidate for constructing inexpensive and advanced environmentally functional materials and energy storage devices.展开更多
Ion cyclotron range of frequency（ICRF） heating has been used in tokamaks as one of the most successful auxiliary heating tools and has been adopted in the EAST. However, the antenna load will fluctuate with the chan...Ion cyclotron range of frequency（ICRF） heating has been used in tokamaks as one of the most successful auxiliary heating tools and has been adopted in the EAST. However, the antenna load will fluctuate with the change of plasma parameters in the ICRF heating process. To ensure the steady operation of the ICRF heating system in the EAST, fast ferrite tuner（FFT） has been carried out to achieve real-time impedance matching. For the requirements of the FFT impedance matching system, the magnet system of the ferrite tuner（FT） was designed by numerical simulations and experimental analysis, where the biasing magnetic circuit and alternating magnetic circuit were the key researched parts of the ferrite magnet. The integral design goal of the FT magnetic circuit is that DC bias magnetic field is 2000 Gs and alternating magnetic field is±400 Gs. In the FTT, E-type magnetic circuit was adopted. Ferrite material is Nd Fe B with a thickness of 30 mm by setting the working point of Nd Fe B, and the ampere turn of excitation coil is 25 through the theoretical calculation and simulation analysis. The coil inductance to generate alternating magnetic field is about 7 m H. Eddy-current effect has been analyzed, while the magnetic field distribution has been measured by a Hall probe in the medium plane of the biasing magnet. Finally, the test results show the good performance of the biasing magnet satisfying the design and operating requirements of the FFT.展开更多
In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents ...In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents and application amount of nitrogen fertilizer on hybrid wheat seed production and its component factors. The results showed that the seed production increased with the increase in the number of female parent row. When the row ratio of male and female parents was 2 ∶ 6,the seed production was 3 683. 8 kg/ha; when the application amount of nitrogen fertilization was 50 kg/ha,the seed production was 3 649. 4 kg/ha; the interaction between the row ratio of male and female parents and the application amount of nitrogen fertilizer indicated that when the row ratio of male and female parents was 2∶ 6 and the application amount of nitrogen fertilizer was 300 kg/ha,the seed production reached the highest( 4160. 6 kg/ha). The row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the component factors of seed production,including the number of grains per spike,spike weight and setting percentage. When the row ratio of male and female parents was 2∶ 5,the number of grains per spike,spike weight and setting percentage were the highest at 26. 7 grains,1. 12 g,and 62. 6% respectively; when application amount of nitrogen fertilizer was 450 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 26. 0 grains,1. 08 g,and59. 2% respectively; the interaction of row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the number of grains per spike,spike weight and setting percentage; when the row ratio of male and female parents was 2∶ 5 and the application amount of nitrogen fertilizer was 300 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 29. 6grains,1. 24 g,and 71. 6% respectively. The number of grains per spike is the largest component factor for seed production. Increasing the number of grains per spike can increase the seed production. According to the effects of row ratio of male and female parents and application amount of nitrogen fertilizer on the component factors of seed production,the optimal condition was 2∶ 5-2∶ 6 for row ratio of male and female parents and 300-450 kg/ha for application amount of nitrogen fertilizer.展开更多
The term“acute-on-chronic liver failure”(ACLF)was initially proposed nearly 30 years ago.[1]ACLF is distinct from decompensated cirrhosis and acute liver failure,representing an independent clinical syndrome resulti...The term“acute-on-chronic liver failure”(ACLF)was initially proposed nearly 30 years ago.[1]ACLF is distinct from decompensated cirrhosis and acute liver failure,representing an independent clinical syndrome resulting from an acute insult on the background of chronic liver disease.[2,3].展开更多
X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression.In female mammals,mono-allelic expression of the long non-coding RNA gene X-inac...X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression.In female mammals,mono-allelic expression of the long non-coding RNA gene X-inactive specific transcript(XIST)is essential for initiation of X chromosome inactivation upon differentiation.We have previously demonstrated that the central factor of super elongation complex-like 3(SEC-L3),AFF3,is enriched at gamete differentially methylated regions(DMRs)of the imprinted loci and regulates the imprinted gene expression.Here,we found that AFF3 can also bind to the DMR downstream of the XIST promoter.Knockdown of AFF3 leads to de-repression of the inactive allele of X IST in terminally differentiated cells.In addition,the binding of AFF3 to the XIST DMR relies on DNA methylation and also regulates DNA methylation level at DMR region.However,the KAP1-H3K9 methylation machineries,which regulate the imprinted loci,might not play major roles in maintaining the mono-aUelic expression pattern of XIST in these cells.Thus,our results suggest that the differential mechanisms involved in the X IST DMR and gDMR regulation,which both require AFF3 and DNA methylation.展开更多
We present an energy penalization method for isogeometric topology optimization using moving morphable components(ITO–MMC),propose an ITO–MMC with an additional bilateral or periodic symmetric constraint for symmetr...We present an energy penalization method for isogeometric topology optimization using moving morphable components(ITO–MMC),propose an ITO–MMC with an additional bilateral or periodic symmetric constraint for symmetric structures,and then extend the proposed energy penalization method to an ITO–MMC with a symmetric constraint.The energy penalization method can solve the problems of numerical instability and convergence for the ITO–MMC and the ITO–MMC subjected to the structural symmetric constraint with asymmetric loads.Topology optimization problems of asymmetric,bilateral symmetric,and periodic symmetric structures are discussed to validate the effectiveness of the proposed energy penalization approach.Compared with the conventional ITO–MMC,the energy penalization method for the ITO–MMC can improve the convergence rate from 18.6%to 44.5%for the optimization of the asymmetric structure.For the ITO–MMC under a bilateral symmetric constraint,the proposed method can reduce the objective value by 5.6%and obtain a final optimized topology that has a clear boundary with decreased iterations.For the ITO–MMC under a periodic symmetric constraint,the proposed energy penalization method can dramatically reduce the number of iterations and obtain a speedup of more than 2.展开更多
Maximizing the fundamental eigenfrequency is an efficient means for vibrating structures to avoid resonance and noises.In this study,we develop an isogeometric analysis(IGA)-based level set model for the fonnulation a...Maximizing the fundamental eigenfrequency is an efficient means for vibrating structures to avoid resonance and noises.In this study,we develop an isogeometric analysis(IGA)-based level set model for the fonnulation and solution of topology optimization in cases with maximum eigenfrequency.The proposed method is based on a combination of level set method and IGA technique,which uses the non-uniform rational B-spline(NURBS),description of geometry,to perfonn analysis.The same NURBS is used for geometry representation,but also for IGA-based dynamic analysis and parameterization of the level set surface,that is,the level set function.The method is applied to topology optimization problems of maximizing the fundamental eigenfrequency for a given amount of material.A modal track method,that monitors a single target eigenmode is employed to prevent the exchange of eigenmode order number in eigenfrequency optimization.The validity and efficiency of the proposed method are illustrated by benchmark examples.展开更多
Background and Aims:Acute-on-chronic liver failure(ACLF)tends to progress rapidly with high short-term mortality.We aimed to create a widely applicable,simple prognostic(WASP)score for ACLF patients.Methods:A retrospe...Background and Aims:Acute-on-chronic liver failure(ACLF)tends to progress rapidly with high short-term mortality.We aimed to create a widely applicable,simple prognostic(WASP)score for ACLF patients.Methods:A retrospective cohort of ACLF cases recruited from three centers in China were divided into training and validation sets to develop the new score.A prospective longitudinal cohort was recruited for further validation.Results:A total of 541 cases were included in the training set,and seven independent ACLF prognostic factors were screened to construct a new quantitative WASP-ACLF table.In the validation set of 671 cases,WASP-ACLF showed better predictive ability for 28-day and 90-day mortality than the currently used prognostic scores at baseline,day 3,week 1,and week 2.The predictive efficacy and clinical validity of the model improved over time.Patients were assigned to low-,intermediate-,and high-risk groups by their WASP-ACLF scores.Compared with the other two groups,intermediate-risk patients had a more uncertain prognosis,with a 90-day mortality of 44.4–50.6%.Sequential assessments at weeks 1 and 2 found the 90-day mortality of intermediate-risk groups was<20%forpatients with a≥2 point decrease in WASP-ACLF and was up to 56%for patients with a≥2 points increase.Similar results were observed in prospective data.Conclusions:The new ACLF prognostic score was simple,widely applicable,and had good predictive efficacy.Continuous assessments and trend of change in WASP-ACLF need to be considered,especially for intermediate-risk patients.展开更多
The flow-through electro-Fenton(EF-T)reactor with WBC cathode was designed to remove florfenicol(FF).The activated WBC cathode was prepared by facile carbonization and activation methods,and featured high specific sur...The flow-through electro-Fenton(EF-T)reactor with WBC cathode was designed to remove florfenicol(FF).The activated WBC cathode was prepared by facile carbonization and activation methods,and featured high specific surface area,natural multi-channel structure,abundant oxygen-containing groups,good hydrophilicity,and excellent O_(2)reducing capacity.WBC cathode was located above Ti/Ru-IrO_(2)mesh anode.O_(2)evolved at the anode was carried to the inner wall of channel of WBC by the force of buoyancy and water flow,which increases oxygen source of H_(2)O_(2)generation at the cathode.The flow-through system by using WBC electrode promote the mass transfer of O_(2)and FF.The production amount of H_(2)O_(2)at activated WBC was 32.2 mg/L,which was almost twice as much as that at non-activated WBC(15.0 mg/L).FF removal ratio in EF-T system was 98%,which was much higher than that of traditional flow-by electro-Fenton(EF-B,33%)or single electrooxidation system(EO,16%).EF-T system has the lowest energy consumption(4.367 kWh/kg)among the three electrochemical systems.The cathodic adsorption,anodic electrooxidation,and EF reaction are responsible for the degradation of FF.After five consecutive cycle experiments,FF removal ratio was still 98%,indicating WBC has the good stability.展开更多
Regulation of RNA stability plays a crucial role in gene expression control.Deadenylation is the initial rate-limiting step for the majority of RNA decay events.Here,we show that RING finger protein 219(RNF219)interac...Regulation of RNA stability plays a crucial role in gene expression control.Deadenylation is the initial rate-limiting step for the majority of RNA decay events.Here,we show that RING finger protein 219(RNF219)interacts with the CCR4-NOT deadenylase complex.RNF219-CCR4-NOT exhibits deadenylation activity in vitro.RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown,while upregulated after depletion of the CCR4-NOT subunit CNOTIO in mouse embryonic stem(ES)cells.RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation.Our study suggests that RNF219 is a novel interacting partner of CCR4-NOT and required for maintenance of ES cell pluripotency.展开更多
基金financial support from the National Natural Science Foundation of China(21908024,22078374 and 52100173)Key Realm Research and Development Program of Guangdong Province(2020B0202080001)+2 种基金Science and Technology Planning Project of Guangdong Province,China(2021B1212040008)Guangdong Laboratory for Lingnan Modern Agriculture Project(NT2021010)Scientific and Technological Planning Project of Guangzhou(202206010145).
文摘Dwindling energy sources and a worsening environment are huge global problems,and biomass wastes are an under-exploited source of material for both energy and material generation.Herein,self-template decoction dregs of Ganoderma lucidum-derived porous carbon nanotubes(ST-DDLGCs)were synthesized via a facile and scalable strategy in response to these challenges.ST-DDLGCs exhibited a large surface area(1731.51 m^(2)g^(-1))and high pore volume(0.76 cm^(3)g^(-1)),due to the interlacing tubular structures of precursors and extra-hierarchical porous structures on tube walls.In the ST-DDLGC/PMS system,the degradation efficiency of capecitabine(CAP)reached~97.3%within 120 min.Moreover,ST-DDLGCs displayed high catalytic activity over a wide pH range of 3–9,and strong anti-interference to these typical and ubiquitous anions in wastewater and natural water bodies(i.e.,H_(2)PO_(4)^(-),NO_(3)^(-),Cl^(-) and HCO_(3)^(-)),in which a ^(1)O_(2)-dominated oxidation was identified and non-radical mechanisms were deduced.Additionally,ST-DDLGC-based coin-type symmetrical supercapacitors exhibited outstanding electrochemical performance,with specific capacitances of up to 328.1 F g^(-1)at 0.5 A g^(-1),and cycling stability of up to 98.6%after 10,000 cycles at a current density of 2 A g^(-1).The superior properties of ST-DDLGCs could be attributed to the unique porous tubular structure,which facilitated mass transfer and presented numerous active sites.The results highlight ST-DDLGCs as a potential candidate for constructing inexpensive and advanced environmentally functional materials and energy storage devices.
基金supported by National Natural Science Foundation of China(Grant No.11575237)the National Magnetic Confinement Fusion Science Program(Grant No.2015GB101001)the International Scientific and Technological Cooperation Project of Anhui(Grant No.1704e1002207)
文摘Ion cyclotron range of frequency（ICRF） heating has been used in tokamaks as one of the most successful auxiliary heating tools and has been adopted in the EAST. However, the antenna load will fluctuate with the change of plasma parameters in the ICRF heating process. To ensure the steady operation of the ICRF heating system in the EAST, fast ferrite tuner（FFT） has been carried out to achieve real-time impedance matching. For the requirements of the FFT impedance matching system, the magnet system of the ferrite tuner（FT） was designed by numerical simulations and experimental analysis, where the biasing magnetic circuit and alternating magnetic circuit were the key researched parts of the ferrite magnet. The integral design goal of the FT magnetic circuit is that DC bias magnetic field is 2000 Gs and alternating magnetic field is±400 Gs. In the FTT, E-type magnetic circuit was adopted. Ferrite material is Nd Fe B with a thickness of 30 mm by setting the working point of Nd Fe B, and the ampere turn of excitation coil is 25 through the theoretical calculation and simulation analysis. The coil inductance to generate alternating magnetic field is about 7 m H. Eddy-current effect has been analyzed, while the magnetic field distribution has been measured by a Hall probe in the medium plane of the biasing magnet. Finally, the test results show the good performance of the biasing magnet satisfying the design and operating requirements of the FFT.
基金Supported by State Key Research and Development Program of Ministry of Science and Technology(2016YFD0101603)Fundamental Research Funds for the Central Universities in 2016(XDJK2016A020)
文摘In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents and application amount of nitrogen fertilizer on hybrid wheat seed production and its component factors. The results showed that the seed production increased with the increase in the number of female parent row. When the row ratio of male and female parents was 2 ∶ 6,the seed production was 3 683. 8 kg/ha; when the application amount of nitrogen fertilization was 50 kg/ha,the seed production was 3 649. 4 kg/ha; the interaction between the row ratio of male and female parents and the application amount of nitrogen fertilizer indicated that when the row ratio of male and female parents was 2∶ 6 and the application amount of nitrogen fertilizer was 300 kg/ha,the seed production reached the highest( 4160. 6 kg/ha). The row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the component factors of seed production,including the number of grains per spike,spike weight and setting percentage. When the row ratio of male and female parents was 2∶ 5,the number of grains per spike,spike weight and setting percentage were the highest at 26. 7 grains,1. 12 g,and 62. 6% respectively; when application amount of nitrogen fertilizer was 450 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 26. 0 grains,1. 08 g,and59. 2% respectively; the interaction of row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the number of grains per spike,spike weight and setting percentage; when the row ratio of male and female parents was 2∶ 5 and the application amount of nitrogen fertilizer was 300 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 29. 6grains,1. 24 g,and 71. 6% respectively. The number of grains per spike is the largest component factor for seed production. Increasing the number of grains per spike can increase the seed production. According to the effects of row ratio of male and female parents and application amount of nitrogen fertilizer on the component factors of seed production,the optimal condition was 2∶ 5-2∶ 6 for row ratio of male and female parents and 300-450 kg/ha for application amount of nitrogen fertilizer.
基金supported by the Beijing Hospitals Authority’s Ascent Plan(No.2022-DFL20221501)Construction Project of High-level Technology Talents in Public Health(No.Discipline leader-01-12)+1 种基金National Key Research and Development Program of China(No.2022YFC2304402)Capital’s Funds for the Health Improvement and Research(No.2024-1-2181).
文摘The term“acute-on-chronic liver failure”(ACLF)was initially proposed nearly 30 years ago.[1]ACLF is distinct from decompensated cirrhosis and acute liver failure,representing an independent clinical syndrome resulting from an acute insult on the background of chronic liver disease.[2,3].
基金Thousand Young Talents Plan of China(5631006003 to C.L.,6231000011 to Z.L.)Natural Science Foundation of Jiangsu Province of China(BK20160026 to C.L.,BK20160666 and BK20170020 to Z.L.)Fundamental Research Funds for the Central Universities(3231007201 to C.L,3231008201 to Z.L.).
文摘X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression.In female mammals,mono-allelic expression of the long non-coding RNA gene X-inactive specific transcript(XIST)is essential for initiation of X chromosome inactivation upon differentiation.We have previously demonstrated that the central factor of super elongation complex-like 3(SEC-L3),AFF3,is enriched at gamete differentially methylated regions(DMRs)of the imprinted loci and regulates the imprinted gene expression.Here,we found that AFF3 can also bind to the DMR downstream of the XIST promoter.Knockdown of AFF3 leads to de-repression of the inactive allele of X IST in terminally differentiated cells.In addition,the binding of AFF3 to the XIST DMR relies on DNA methylation and also regulates DNA methylation level at DMR region.However,the KAP1-H3K9 methylation machineries,which regulate the imprinted loci,might not play major roles in maintaining the mono-aUelic expression pattern of XIST in these cells.Thus,our results suggest that the differential mechanisms involved in the X IST DMR and gDMR regulation,which both require AFF3 and DNA methylation.
基金This research was supported by the National Natural Science Foundation of China(Grant Nos.51675197 and 51705158)the National Engineering Research Center of Near-Net-Shape Forming for Metallic Materials,Ministry of Education Key Laboratory of High Efficient Near-Net-Shape Forming Technology and Equipment for Metallic Materials(Category B)Opening Foundation(Grant No.2018005)The support is gratefully acknowledged.
文摘We present an energy penalization method for isogeometric topology optimization using moving morphable components(ITO–MMC),propose an ITO–MMC with an additional bilateral or periodic symmetric constraint for symmetric structures,and then extend the proposed energy penalization method to an ITO–MMC with a symmetric constraint.The energy penalization method can solve the problems of numerical instability and convergence for the ITO–MMC and the ITO–MMC subjected to the structural symmetric constraint with asymmetric loads.Topology optimization problems of asymmetric,bilateral symmetric,and periodic symmetric structures are discussed to validate the effectiveness of the proposed energy penalization approach.Compared with the conventional ITO–MMC,the energy penalization method for the ITO–MMC can improve the convergence rate from 18.6%to 44.5%for the optimization of the asymmetric structure.For the ITO–MMC under a bilateral symmetric constraint,the proposed method can reduce the objective value by 5.6%and obtain a final optimized topology that has a clear boundary with decreased iterations.For the ITO–MMC under a periodic symmetric constraint,the proposed energy penalization method can dramatically reduce the number of iterations and obtain a speedup of more than 2.
基金the National Natural Science Foundation of China(Grant No.51675197).
文摘Maximizing the fundamental eigenfrequency is an efficient means for vibrating structures to avoid resonance and noises.In this study,we develop an isogeometric analysis(IGA)-based level set model for the fonnulation and solution of topology optimization in cases with maximum eigenfrequency.The proposed method is based on a combination of level set method and IGA technique,which uses the non-uniform rational B-spline(NURBS),description of geometry,to perfonn analysis.The same NURBS is used for geometry representation,but also for IGA-based dynamic analysis and parameterization of the level set surface,that is,the level set function.The method is applied to topology optimization problems of maximizing the fundamental eigenfrequency for a given amount of material.A modal track method,that monitors a single target eigenmode is employed to prevent the exchange of eigenmode order number in eigenfrequency optimization.The validity and efficiency of the proposed method are illustrated by benchmark examples.
基金supported primarily by research grants from the National 13th 5-Year Plan for Hepatitis Research(No.2017ZX10203201-007)the National Natural Science Foundation of China(No.81870429,82170630)the Natural Science Foundation of Tianjin(No.19JCZDJC36700).
文摘Background and Aims:Acute-on-chronic liver failure(ACLF)tends to progress rapidly with high short-term mortality.We aimed to create a widely applicable,simple prognostic(WASP)score for ACLF patients.Methods:A retrospective cohort of ACLF cases recruited from three centers in China were divided into training and validation sets to develop the new score.A prospective longitudinal cohort was recruited for further validation.Results:A total of 541 cases were included in the training set,and seven independent ACLF prognostic factors were screened to construct a new quantitative WASP-ACLF table.In the validation set of 671 cases,WASP-ACLF showed better predictive ability for 28-day and 90-day mortality than the currently used prognostic scores at baseline,day 3,week 1,and week 2.The predictive efficacy and clinical validity of the model improved over time.Patients were assigned to low-,intermediate-,and high-risk groups by their WASP-ACLF scores.Compared with the other two groups,intermediate-risk patients had a more uncertain prognosis,with a 90-day mortality of 44.4–50.6%.Sequential assessments at weeks 1 and 2 found the 90-day mortality of intermediate-risk groups was<20%forpatients with a≥2 point decrease in WASP-ACLF and was up to 56%for patients with a≥2 points increase.Similar results were observed in prospective data.Conclusions:The new ACLF prognostic score was simple,widely applicable,and had good predictive efficacy.Continuous assessments and trend of change in WASP-ACLF need to be considered,especially for intermediate-risk patients.
基金supported by the National Science Fund for Distinguished Young Scholars(No.51625801)Major Science and Technology Program for Water Pollution Control and Treatment in China(No.2017ZX07202-001-007)+4 种基金Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2017)Guangdong Provincial Science and Technology Project(No.2017A020216014)the National Natural Science Foundation of China(No.21777106)Pearl River S&T Nova Program of Guangzhou,China(No.201710010065)Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology(No.2020B1212060022)。
文摘The flow-through electro-Fenton(EF-T)reactor with WBC cathode was designed to remove florfenicol(FF).The activated WBC cathode was prepared by facile carbonization and activation methods,and featured high specific surface area,natural multi-channel structure,abundant oxygen-containing groups,good hydrophilicity,and excellent O_(2)reducing capacity.WBC cathode was located above Ti/Ru-IrO_(2)mesh anode.O_(2)evolved at the anode was carried to the inner wall of channel of WBC by the force of buoyancy and water flow,which increases oxygen source of H_(2)O_(2)generation at the cathode.The flow-through system by using WBC electrode promote the mass transfer of O_(2)and FF.The production amount of H_(2)O_(2)at activated WBC was 32.2 mg/L,which was almost twice as much as that at non-activated WBC(15.0 mg/L).FF removal ratio in EF-T system was 98%,which was much higher than that of traditional flow-by electro-Fenton(EF-B,33%)or single electrooxidation system(EO,16%).EF-T system has the lowest energy consumption(4.367 kWh/kg)among the three electrochemical systems.The cathodic adsorption,anodic electrooxidation,and EF reaction are responsible for the degradation of FF.After five consecutive cycle experiments,FF removal ratio was still 98%,indicating WBC has the good stability.
基金Studies in this manuscript were supported by funds provided by the National Natural Science Foundation of China(31671343 and 31970617 to C.L.,31970626 to Z.L.,31700718 to D.H.)National Key R&D Program of China(2018YFA0800100 to C.L.)+2 种基金Natural Science Foundation of Jiangsu Province of China(BK20170020 to Z.L.,BK20170663 to D.H.)China Postdoctoral Science Foundation(2018M630492 to D.H.)Scientific Research Foundation of the Graduate School of Southeast University(YBPY1888 to Y.W.).
文摘Regulation of RNA stability plays a crucial role in gene expression control.Deadenylation is the initial rate-limiting step for the majority of RNA decay events.Here,we show that RING finger protein 219(RNF219)interacts with the CCR4-NOT deadenylase complex.RNF219-CCR4-NOT exhibits deadenylation activity in vitro.RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown,while upregulated after depletion of the CCR4-NOT subunit CNOTIO in mouse embryonic stem(ES)cells.RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation.Our study suggests that RNF219 is a novel interacting partner of CCR4-NOT and required for maintenance of ES cell pluripotency.
