An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat(SSR) marker-assisted selection(MAS). Z550 carr...An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat(SSR) marker-assisted selection(MAS). Z550 carries 13 substitution segments distributed on chromosomes 1, 6, 7, 8, 9, 10, and 12, with an average substitution length of 1.68 Mb. Compared with the Nipponbare parental line, plant height, panicle length, spikelets per panicle, grains per panicle, and grain weight for Z550 were significantly increased. While the grain width of Z550 was significantly narrower, and the seed setting ratio(81.43%) was significantly lower than that of Nipponbare, it is still sufficient for breeding purposes. Quantitative trait loci(QTLs) mapping for important agronomic traits was conducted with the F_2 population derived from Nipponbare crossed with Z550 using the restricted maximum likelihood(REML) method. A total of 16, including 12 previously unreported QTLs were detected, with contribution rates ranging from 1.46 to 10.49%. Grains per panicle was controlled by 8 QTLs, 5 of which increased number of grains whereas 3 decreased it. qGPP-1, with the largest contribution(10.49%), was estimated to increase grains per panicle by 30.67, while q GPP-9, with the minimum contribution rate(2.47%), had an effect of increasing grains per panicle by 15.79. These results will be useful for further development of single segment substitution lines with major QTLs, and for research of their molecular functions via QTL cloning.展开更多
动态基因调控网是展现生物体内基因与基因之间相互关系随时间变化而变化的动力学行为的复杂网络.这种相互作用关系可以分为两类:激励和抑制.对动态基因调控网网络演化的研究,可以预测未来时刻生物体内的基因调控关系,从而在疾病预测和...动态基因调控网是展现生物体内基因与基因之间相互关系随时间变化而变化的动力学行为的复杂网络.这种相互作用关系可以分为两类:激励和抑制.对动态基因调控网网络演化的研究,可以预测未来时刻生物体内的基因调控关系,从而在疾病预测和诊断、药物开发、生物学实验等领域起到重要的指导和辅助作用.现实世界中,动态基因调控网的网络演化是一个复杂而巨大的系统,当前,对于其演化机制的研究存在只关注静态网络而忽略动态网络和只关注相互作用关系而忽略相互作用类型的缺陷.针对上述问题,提出了一种动态基因调控网演化分析方法(dynamic gene regulatory network evolution analyzing method,简称DGNE),将研究扩展到了动态带符号网络领域.通过该方法包含的基于模体转换概率的连边预测算法(link prediction algorithm based on motif transfer probability,简称MT)和基于隐空间特征的符号判别算法,能够动态地捕捉基因调控网的演化机制,并准确地预测未来时刻基因调控网的连边情况.实验结果表明,DGNE方法在仿真数据集和真实数据集上均有良好的表现.展开更多
Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified...Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified. Z744 contains a total of six substitution segments distributed on chromosomes(Chrs.) 1, 2, 6, 7, and 12, with an average substitution length of 2.72 Mb. The grain length, ratio of length to width, and 1 000-grain weight of Z744 were significantly higher than those in Nipponbare. The plant height, panicle number, and seed-set ratio in Z744 were significantly lower than those in Nipponbare, but they were still 78.7 cm, 13.5 per plant, and 86.49%, respectively. Furthermore, eight QTLs of different traits were identified in the secondary F2 population, constructed by Nipponbare and Z744 hybridization. The grain weight of Z744 was controlled by two synergistic QTLs(qGWT1 and q GWT7) and two subtractive QTLs(qGWT2 and qGWT6), respectively. The increase in the grain weight of Z744 was caused mainly by the increase in grain length. Two QTLs were detected, qGL1 and qGL7-3, which accounted for 25.54 and 15.58% of phenotypic variation, respectively. A Chi-square test showed that the long-grain number and the short-grain number were in accordance with the 3:1 separation ratio, which indicates that the long grain is dominant over the short-grain and Z744 was controlled mainly by the principal effect qGL1. These results offered a good basis for further fine mapping of qGL1 and further dissection of other QTLs into single-segment substitution lines.展开更多
基金supported by the National Key R&D Program of China (2017YFD0100202)the Chongqing Science and Technology Commission Special Project, China (cstc2016shms-ztzx0017)the Southwestern University Basic Operating Expenses Special Innovation Team Project, China (XDJK2017A004)
文摘An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat(SSR) marker-assisted selection(MAS). Z550 carries 13 substitution segments distributed on chromosomes 1, 6, 7, 8, 9, 10, and 12, with an average substitution length of 1.68 Mb. Compared with the Nipponbare parental line, plant height, panicle length, spikelets per panicle, grains per panicle, and grain weight for Z550 were significantly increased. While the grain width of Z550 was significantly narrower, and the seed setting ratio(81.43%) was significantly lower than that of Nipponbare, it is still sufficient for breeding purposes. Quantitative trait loci(QTLs) mapping for important agronomic traits was conducted with the F_2 population derived from Nipponbare crossed with Z550 using the restricted maximum likelihood(REML) method. A total of 16, including 12 previously unreported QTLs were detected, with contribution rates ranging from 1.46 to 10.49%. Grains per panicle was controlled by 8 QTLs, 5 of which increased number of grains whereas 3 decreased it. qGPP-1, with the largest contribution(10.49%), was estimated to increase grains per panicle by 30.67, while q GPP-9, with the minimum contribution rate(2.47%), had an effect of increasing grains per panicle by 15.79. These results will be useful for further development of single segment substitution lines with major QTLs, and for research of their molecular functions via QTL cloning.
文摘动态基因调控网是展现生物体内基因与基因之间相互关系随时间变化而变化的动力学行为的复杂网络.这种相互作用关系可以分为两类:激励和抑制.对动态基因调控网网络演化的研究,可以预测未来时刻生物体内的基因调控关系,从而在疾病预测和诊断、药物开发、生物学实验等领域起到重要的指导和辅助作用.现实世界中,动态基因调控网的网络演化是一个复杂而巨大的系统,当前,对于其演化机制的研究存在只关注静态网络而忽略动态网络和只关注相互作用关系而忽略相互作用类型的缺陷.针对上述问题,提出了一种动态基因调控网演化分析方法(dynamic gene regulatory network evolution analyzing method,简称DGNE),将研究扩展到了动态带符号网络领域.通过该方法包含的基于模体转换概率的连边预测算法(link prediction algorithm based on motif transfer probability,简称MT)和基于隐空间特征的符号判别算法,能够动态地捕捉基因调控网的演化机制,并准确地预测未来时刻基因调控网的连边情况.实验结果表明,DGNE方法在仿真数据集和真实数据集上均有良好的表现.
基金supported by the National Natural Science Foundation of China(31871593)the Chongqing Science and Technology Commission Special Project,China(cstc2016shms-ztzx0032)the Southwest University Innovation Team Project,China(XDJK2017A004)。
文摘Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified. Z744 contains a total of six substitution segments distributed on chromosomes(Chrs.) 1, 2, 6, 7, and 12, with an average substitution length of 2.72 Mb. The grain length, ratio of length to width, and 1 000-grain weight of Z744 were significantly higher than those in Nipponbare. The plant height, panicle number, and seed-set ratio in Z744 were significantly lower than those in Nipponbare, but they were still 78.7 cm, 13.5 per plant, and 86.49%, respectively. Furthermore, eight QTLs of different traits were identified in the secondary F2 population, constructed by Nipponbare and Z744 hybridization. The grain weight of Z744 was controlled by two synergistic QTLs(qGWT1 and q GWT7) and two subtractive QTLs(qGWT2 and qGWT6), respectively. The increase in the grain weight of Z744 was caused mainly by the increase in grain length. Two QTLs were detected, qGL1 and qGL7-3, which accounted for 25.54 and 15.58% of phenotypic variation, respectively. A Chi-square test showed that the long-grain number and the short-grain number were in accordance with the 3:1 separation ratio, which indicates that the long grain is dominant over the short-grain and Z744 was controlled mainly by the principal effect qGL1. These results offered a good basis for further fine mapping of qGL1 and further dissection of other QTLs into single-segment substitution lines.
