Sepsis is characterized by a severe and life-threatening host immune response to polymicrobial infection accompanied by organ dysfunction.Studies on the therapeutic effect and mechanism of immunomodulatory drugs on th...Sepsis is characterized by a severe and life-threatening host immune response to polymicrobial infection accompanied by organ dysfunction.Studies on the therapeutic effect and mechanism of immunomodulatory drugs on the sepsis-induced hyperinflammatory or immunosuppression states of various immune cells remain limited.This study aimed to investigate the protective effects and underlying mechanism of artesunate(ART)on the splenic microenvironment of cecal ligation and puncture-induced sepsis model mice using single-cell RNA sequencing(scRNA-seq)and experimental validations.The scRNA-seq analysis revealed that ART inhibited the activation of pro-inflammatory macrophages recruited during sepsis.ART could restore neutrophils’chemotaxis and immune function in the septic spleen.It inhibited the activation of T regulatory cells but promoted the cytotoxic function of natural killer cells during sepsis.ART also promoted the differentiation and activity of splenic B cells in mice with sepsis.These results indicated that ART could alleviate the inflammatory and/or immunosuppressive states of various immune cells involved in sepsis to balance the immune homeostasis within the host.Overall,this study provided a comprehensive investigation of the regulatory effect of ART on the splenic microenvironment in sepsis,thus contributing to the application of ART as adjunctive therapy for the clinical treatment of sepsis.展开更多
Diabetic nephropathy(DN)has become the leading cause of end-stage renal disease with high morbidity and mortality among individuals with diabetes mellitus.Although functional alterations of renal infiltrating immune c...Diabetic nephropathy(DN)has become the leading cause of end-stage renal disease with high morbidity and mortality among individuals with diabetes mellitus.Although functional alterations of renal infiltrating immune cells have been reported as part of the pathological mechanism of DN,the understanding of the immune response underlying peripheral blood mononuclear cells(PBMCs)in DN remains limited.Here,single-cell RNA sequencing(scRNA-seq)was used toprofile the transcriptomic signatures of PBMCs from DN patients.展开更多
Few-layers WS_(2) was obtained through unique chemical liquid exfoliation of commercial WS_(2).Results showed that after the exfoliation process,the thickness of WS_(2) reduced significantly.Moreover,the NiFe_(2)O_(4)...Few-layers WS_(2) was obtained through unique chemical liquid exfoliation of commercial WS_(2).Results showed that after the exfoliation process,the thickness of WS_(2) reduced significantly.Moreover,the NiFe_(2)O_(4) nanosheets/WS_(2) composite was successfully synthesized through a facile hydrothermal method at 180°C,and then proven by the analyses of field emission scanning electron microscopy,X-ray diffraction,and X-ray photoelectron spectroscopy.The composite showed a high specific surface area of 86.89 m^(2)·g^(−1) with an average pore size of 3.13 nm.Besides,in the threeelectrode electrochemical test,this composite exhibited a high specific capacitance of 878.04 F·g^(−1) at a current density of 1 A·g^(−1),while in the two-electrode system,the energy density of the composite could reach 25.47 Wh·kg^(−1) at the power density of 70 W·kg^(−1) and maintained 13.42 Wh·kg^(−1) at the higher power density of 7000 W·kg^(−1).All the excellent electrochemical performances demonstrate that the NiFe_(2)O_(4) nanosheets/WS_(2) composite is an excellent candidate for supercapacitor applications.展开更多
NiFe_(2)O_(4) is a kind of bimetallic oxide possessing excellent theoretical capacity and application prospect in the field of supercapacitors.Whereas,due to the inherent poor conductivity of metal oxides,the performa...NiFe_(2)O_(4) is a kind of bimetallic oxide possessing excellent theoretical capacity and application prospect in the field of supercapacitors.Whereas,due to the inherent poor conductivity of metal oxides,the performance of NiFe_(2)O_(4) is not ideal in practice.Oxygen vacancies can not only enhance the conductivities of NiFe_(2)O_(4) but also provide better adsorption of OH,which is beneficial to the electrochemical performances.Hence,oxygen vacancies engineered NiFe_(2)O_(4)(NiFe_(2)O_(4)‒δ)is obtained through a two-step method,including a hydrothermal reaction and a further heat treatment in activated carbon bed.Results of electron paramagnetic resonance spectra indicate that more oxygen vacancies exist in the treated NiFe_(2)O_(4)‒δthan the original one.UV-Vis diffuse reflectance spectra prove that the treated NiFe_(2)O_(4)‒δowns better conductivity than the original NiFe_(2)O_(4).As for the electrochemical performances,the treated NiFe_(2)O_(4)‒δperforms a high specific capacitance of 808.02 F∙g^(‒1) at 1 A∙g^(‒1).Moreover,the asymmetric supercapacitor of NiFe_(2)O_(4)‒δ//active carbon displays a high energy density of 17.7 Wh∙kg^(‒1) at the power density of 375 W∙kg^(‒1).This work gives an effective way to improve the conductivity of metal oxides,which is beneficial to the application of metal oxides in supercapacitors.展开更多
基金support by the Establishment of Sino-Austria“Belt and Road”Joint Laboratory on Traditional Chinese Medicine for Severe Infectious Diseases and Joint Research,China(Grant No.:2020YFE0205100)the National Key Research and Development Program of China(Grant Nos.:2020YFA0908000,2022YFC2303600)+9 种基金the Distinguished Expert Project of Sichuan Province Tianfu Scholar(Grant No.:CW202002)the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine,China(Grant No.:ZYYCXTD-C-202002)the National Natural Science Foundation of China(Grant Nos.:82141001,82274182,82074098,82173914)the China Academy of Chinese Medical Sciences(CACMS)Innovation Fund,China(Grant Nos.:CI2021A05101,CI2021A05104)the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(Grant No.:CI2021B014)the Science and Technology Foundation of Shenzhen,China(Grant No.:JCYJ20210324115800001)the Science and Technology Foundation of Shenzhen,China(Shenzhen Clinical Medical Research Center for Geriatric Diseases),the National Key R&D Program of China Key Projects for International Cooperation on Science,Technology and Innovation(Grant No.:2020YFE0205100)the Fundamental Research Funds for the Central Public Welfare Research Institutes,China(Grant Nos.:ZZ14-YQ-050,ZZ14-YQ-051,ZZ14-YQ-052,ZZ14-FL-002,ZZ14-ND-010,ZZ15-ND-10),Shenzhen Governmental Sustainable Development Fund,China(Grant No.:KCXFZ20201221173612034)Shenzhen key Laboratory of Kidney Diseases,China(Grant No.:ZDSYS201504301616234)Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties,China(Grant No.:SZGSP001).
文摘Sepsis is characterized by a severe and life-threatening host immune response to polymicrobial infection accompanied by organ dysfunction.Studies on the therapeutic effect and mechanism of immunomodulatory drugs on the sepsis-induced hyperinflammatory or immunosuppression states of various immune cells remain limited.This study aimed to investigate the protective effects and underlying mechanism of artesunate(ART)on the splenic microenvironment of cecal ligation and puncture-induced sepsis model mice using single-cell RNA sequencing(scRNA-seq)and experimental validations.The scRNA-seq analysis revealed that ART inhibited the activation of pro-inflammatory macrophages recruited during sepsis.ART could restore neutrophils’chemotaxis and immune function in the septic spleen.It inhibited the activation of T regulatory cells but promoted the cytotoxic function of natural killer cells during sepsis.ART also promoted the differentiation and activity of splenic B cells in mice with sepsis.These results indicated that ART could alleviate the inflammatory and/or immunosuppressive states of various immune cells involved in sepsis to balance the immune homeostasis within the host.Overall,this study provided a comprehensive investigation of the regulatory effect of ART on the splenic microenvironment in sepsis,thus contributing to the application of ART as adjunctive therapy for the clinical treatment of sepsis.
基金supported by the Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties(China)(No.SZGSP001)Shenzhen Governmental Sustainable Development Fund(Guangdong,China)(No.KCXFZ20201221173612034)+3 种基金the National Natural Science Foundation of China(China)(No.82170842)the Natural ScienceFoundation of Shenzhen City(China)(No.KCXFZ20201221173600001)Guangdong Basic and Applied Basic Research Foundation(China)(No.2021A1515012164)Shenzhen Key Laboratory of Kidney Diseases(No.ZDSYS20150430161-6234)。
文摘Diabetic nephropathy(DN)has become the leading cause of end-stage renal disease with high morbidity and mortality among individuals with diabetes mellitus.Although functional alterations of renal infiltrating immune cells have been reported as part of the pathological mechanism of DN,the understanding of the immune response underlying peripheral blood mononuclear cells(PBMCs)in DN remains limited.Here,single-cell RNA sequencing(scRNA-seq)was used toprofile the transcriptomic signatures of PBMCs from DN patients.
基金supported by Major Basic Research Projects of Shandong Natural Science Foundation(ZR2018ZB0104)Science and Technology Development Project of Shandong Province(2016GGX102003 and 2017GGX20105)Natural Science Foundation of Shandong Province(ZR2017BEM032).
文摘Few-layers WS_(2) was obtained through unique chemical liquid exfoliation of commercial WS_(2).Results showed that after the exfoliation process,the thickness of WS_(2) reduced significantly.Moreover,the NiFe_(2)O_(4) nanosheets/WS_(2) composite was successfully synthesized through a facile hydrothermal method at 180°C,and then proven by the analyses of field emission scanning electron microscopy,X-ray diffraction,and X-ray photoelectron spectroscopy.The composite showed a high specific surface area of 86.89 m^(2)·g^(−1) with an average pore size of 3.13 nm.Besides,in the threeelectrode electrochemical test,this composite exhibited a high specific capacitance of 878.04 F·g^(−1) at a current density of 1 A·g^(−1),while in the two-electrode system,the energy density of the composite could reach 25.47 Wh·kg^(−1) at the power density of 70 W·kg^(−1) and maintained 13.42 Wh·kg^(−1) at the higher power density of 7000 W·kg^(−1).All the excellent electrochemical performances demonstrate that the NiFe_(2)O_(4) nanosheets/WS_(2) composite is an excellent candidate for supercapacitor applications.
基金supported by Major Basic Research Projects of Shandong Natural Science Foundation(Grant No.ZR2018ZB0104)Science and Technology Development Project of Shandong Province(Grant Nos.2016GGX102003 and 2017GGX20105)Natural Science Foundation of Shandong Province(Grant No.ZR2017BEM032).
文摘NiFe_(2)O_(4) is a kind of bimetallic oxide possessing excellent theoretical capacity and application prospect in the field of supercapacitors.Whereas,due to the inherent poor conductivity of metal oxides,the performance of NiFe_(2)O_(4) is not ideal in practice.Oxygen vacancies can not only enhance the conductivities of NiFe_(2)O_(4) but also provide better adsorption of OH,which is beneficial to the electrochemical performances.Hence,oxygen vacancies engineered NiFe_(2)O_(4)(NiFe_(2)O_(4)‒δ)is obtained through a two-step method,including a hydrothermal reaction and a further heat treatment in activated carbon bed.Results of electron paramagnetic resonance spectra indicate that more oxygen vacancies exist in the treated NiFe_(2)O_(4)‒δthan the original one.UV-Vis diffuse reflectance spectra prove that the treated NiFe_(2)O_(4)‒δowns better conductivity than the original NiFe_(2)O_(4).As for the electrochemical performances,the treated NiFe_(2)O_(4)‒δperforms a high specific capacitance of 808.02 F∙g^(‒1) at 1 A∙g^(‒1).Moreover,the asymmetric supercapacitor of NiFe_(2)O_(4)‒δ//active carbon displays a high energy density of 17.7 Wh∙kg^(‒1) at the power density of 375 W∙kg^(‒1).This work gives an effective way to improve the conductivity of metal oxides,which is beneficial to the application of metal oxides in supercapacitors.
