Dear Editor,The CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9）system is revolutionizing genome editing due to its high efficiency,low cost,design simplicity and ve...Dear Editor,The CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9）system is revolutionizing genome editing due to its high efficiency,low cost,design simplicity and versatility.However,introduction of a point mutation at a desired position remains a great challenge in plant genome engineering.展开更多
Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control.We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of the ac...Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control.We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of the acetolactate synthase(ALS)gene to confer herbicide tolerance in rice.The four different missense mutations in the P171 codon,P171S,P171A,P171Yand P171F,exhibited different patterns of tolerance towards five representative herbicides from five chemical families of ALS inhibitors.For example,P171S and P171A had lower levels of tolerance than P171Y and P171F to bispyribac but not to the other herbicides.Interestingly,a novel triple mutant(P171F/G628E/G629S)had the highest tolerance to all five tested herbicides.Field trials showed that both P171F and P171F/G628E/G629S could potentially be used with nicosulfuron.Our work illustrates an effective way of using base editing to generate herbicide tolerance in elite rice varieties.展开更多
Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double strand...Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double stranded breaks(DSB)were effectively repaired by non-homologous end joining(NHEJ)pathway in higher eukaryotic cells,including animals and plants.展开更多
This paper contains an error in Figure 2, where P171Y and P171A under treatment of pyroxsulam have a duplicated picture.This picture is for P171Y but not for P171A.We provide the correct picture for P171A as follows. ...This paper contains an error in Figure 2, where P171Y and P171A under treatment of pyroxsulam have a duplicated picture.This picture is for P171Y but not for P171A.We provide the correct picture for P171A as follows. This new picture does not affect the conclusion of this article.展开更多
Dear Editor,Horizontal gene transfer(HGT)has long been recognized as asexual means of DNA transfer between different species.Although HGT is common to prokaryotes,it remains uncommon in higher eukaryotic species(Beiko...Dear Editor,Horizontal gene transfer(HGT)has long been recognized as asexual means of DNA transfer between different species.Although HGT is common to prokaryotes,it remains uncommon in higher eukaryotic species(Beiko et al.,2005).展开更多
基金supported by the National Crop Breeding Fund (2016YFD0101804)National Natural Science Foundation of China (31471785, 31670371)Special Fund for Agro-scientific Research in the Public Interest (201303022)
文摘Dear Editor,The CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9）system is revolutionizing genome editing due to its high efficiency,low cost,design simplicity and versatility.However,introduction of a point mutation at a desired position remains a great challenge in plant genome engineering.
基金supported by grants from the National Key R&D Program of China(2018YFA0900600)the National Natural Science Foundation of China(31900301,31872933 and 31971370)the Chinese Academy of Sciences(QYZDY-SSW-SMC030).
文摘Herbicide-tolerant rice varieties generated by genome editing are highly desirable for weed control.We have used a cytosine base editor to create a series of missense mutations in the P171 and/or G628 codons of the acetolactate synthase(ALS)gene to confer herbicide tolerance in rice.The four different missense mutations in the P171 codon,P171S,P171A,P171Yand P171F,exhibited different patterns of tolerance towards five representative herbicides from five chemical families of ALS inhibitors.For example,P171S and P171A had lower levels of tolerance than P171Y and P171F to bispyribac but not to the other herbicides.Interestingly,a novel triple mutant(P171F/G628E/G629S)had the highest tolerance to all five tested herbicides.Field trials showed that both P171F and P171F/G628E/G629S could potentially be used with nicosulfuron.Our work illustrates an effective way of using base editing to generate herbicide tolerance in elite rice varieties.
基金supported by the National Transgenic Science and Technology Program(2019ZX08010-003)National Natural Science Foundation of China(31872933)。
文摘Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double stranded breaks(DSB)were effectively repaired by non-homologous end joining(NHEJ)pathway in higher eukaryotic cells,including animals and plants.
文摘This paper contains an error in Figure 2, where P171Y and P171A under treatment of pyroxsulam have a duplicated picture.This picture is for P171Y but not for P171A.We provide the correct picture for P171A as follows. This new picture does not affect the conclusion of this article.
基金supported by the National Natural Science Foundation of China(31872933 and 31272055)the National Transgenic Science and Technology Program(2019ZX08010-003)。
文摘Dear Editor,Horizontal gene transfer(HGT)has long been recognized as asexual means of DNA transfer between different species.Although HGT is common to prokaryotes,it remains uncommon in higher eukaryotic species(Beiko et al.,2005).
