AIM: To observe the therapeutic effects of dexamethasone on rats with severe acute pancreatitis (SAP) and investigate the influences of dexamethasone on the inflammatory mediators and NF-κB expression in multiple org...AIM: To observe the therapeutic effects of dexamethasone on rats with severe acute pancreatitis (SAP) and investigate the influences of dexamethasone on the inflammatory mediators and NF-κB expression in multiple organs of SAP rats as well as the mechanisms involved. METHODS: Ninety Sprague-Dawley (SD) rats with SAP were randomly divided into the model group (n = 45) and dexamethasone treatment group (n = 45), and another 45 rats were selected for the sham operation group. All groups were randomly subdivided into the 3 h, 6 h and 12 h groups, each group containing 15 rats. The survival of all groups and pathological changes of multiple organs (liver, kidney and lung) were observed at different time points after the operation. The pathologicalscore of multiple organs was carried out, followed by the determination of amylase, endotoxin and TNF-α contents in blood. The tissue microarray was used to detect the expression levels of NF-κB p65 protein in multiple organs. RESULTS: There was no marked difference between the model group and treatment group in the survival rate. The amylase content of the treatment group was significantly lower compared to the model group at 12 h (P < 0.01, 7791.00 vs 9195.00). Moreover, the endotoxin and TNF-α levels of the treatment group were significantly lower than that of the model group at 6 h and 12 h (P < 0.01, 0.040 vs 0.055, 0.042 vs 0.059 and P < 0.05, 58.30 vs 77.54, 38.70 vs 67.30, respectively). Regarding the changes in liver NF-κB expression, the model group significantly exceeded the sham operation group at 3 h (P < 0.01, 1.00 vs 0.00), and the treatment group significantly exceeded the sham operation group at 12 h (P < 0.01, 1.00 vs 0.00), whereas no marked difference was observed between the model group and treatment group at all time points. The kidney NF-κB expression level in the treatment group significantly exceeded the model group (P < 0.05, 2.00 vs 0.00) and the sham operation group (P < 0.01, 2.00 vs 0.00) at 12 h. No NF-κB expression in the lung was found in any group. CONCLUSION: Dexamethasone can lower the amylase, endotoxin and TNF-α levels as well as mortality of SAP rats. NF-κB plays an important role in multiple organ injury. Further studies should be conducted to determine whether dexamethasone can ameliorate the pathological changes of multiple organs by reducing the NF-κB expression in the liver and kidney. The advantages of tissue microarrays in pancreatitis pathological examination include time- and energy- saving, and are highly efficient and representative. The restriction of tissue microarrays on the representation of tissues to various extents due to small diameter may lead to the deviation of analysis.展开更多
The solar radio signal that can be received by the ground-based telescopes covers a wide frequency range,allowing us to monitor the complex physical processes occurred from the solar surface to the vast interplanetary...The solar radio signal that can be received by the ground-based telescopes covers a wide frequency range,allowing us to monitor the complex physical processes occurred from the solar surface to the vast interplanetary space.MingantU SpEctral Radioheliograph(MUSER),as the latest generation of solar dedicated radio spectral-imaging instrument in the centimeter-decimeter wavelengths,has accumulated a large number of observational data since its commissioning observation in 2014.This paper presents the main observational results identified by MUSER from 2014 to 2019,including the quiet Sun and 94 solar radio burst events.We find that there are 81 events accompanied with Geostationary Operational Environmental Satellites(GOES)soft X-ray(SXR)flares,among which the smallest flare class is B1.0.There are 13 events without accompanying any recorded flares,among which the smallest SXR intensity during the radio burst period is equivalent to level-A.The main characteristics of all radio burst events are presented,which shows the powerful ability of MUSER to capture the valuable information of the solar non-thermal processes and the importance for space weather.This work also provides a database for further in-depth research.展开更多
The very low frequency(VLF)regime below 30 MHz in the electromagnetic spectrum has presently been drawing global attention in radio astronomical research due to its potentially significant science outcomes exploring m...The very low frequency(VLF)regime below 30 MHz in the electromagnetic spectrum has presently been drawing global attention in radio astronomical research due to its potentially significant science outcomes exploring many unknown extragalactic sources,transients,and so on.However,the nontransparency of the Earth’s ionosphere,ionospheric distortion and artificial radio frequency interference(RFI)have made it difficult to detect the VLF celestial radio emission with ground-based instruments.A straightforward solution to overcome these problems is a space-based VLF radio telescope,just like the VLF radio instruments onboard the Chang’E-4 spacecraft.But building such a space telescope would be inevitably costly and technically challenging.The alternative approach would be then a ground-based VLF radio telescope.Particularly,in the period of post 2020 when the solar and terrestrial ionospheric activities are expected to be in a’calm’state,it will provide us a good chance to perform VLF ground-based radio observations.Anticipating such an opportunity,we built an agile VLF radio spectrum explorer co-located with the currently operational Mingantu Spectra Radio Heliograph(MUSER).The instrument includes four antennas operating in the VLF frequency range 1-70 MHz.Along with them,we employ an eight-channel analog and digital receivers to amplify,digitize and process the radio signals received by the antennas.We present in the paper this VLF radio spectrum explorer and the instrument will be useful for celestial studies of VLF radio emissions.展开更多
基金Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province, NO. 2003C130 and NO. 2004C142 Foundation Project for Medical Science and Technology of Zhejiang province, No. 2003B134+3 种基金 Grave Foundation Project for Technological and Development of Hangzhou, No. 2003123B19 Intensive Foundation Project for Technology of Hangzhou, NO. 2004Z006 Foundation Project for Medical Science and Technology of Hangzhou, No. 2003A004 and Foundation Project for Technology of Hangzhou, No. 2005224
文摘AIM: To observe the therapeutic effects of dexamethasone on rats with severe acute pancreatitis (SAP) and investigate the influences of dexamethasone on the inflammatory mediators and NF-κB expression in multiple organs of SAP rats as well as the mechanisms involved. METHODS: Ninety Sprague-Dawley (SD) rats with SAP were randomly divided into the model group (n = 45) and dexamethasone treatment group (n = 45), and another 45 rats were selected for the sham operation group. All groups were randomly subdivided into the 3 h, 6 h and 12 h groups, each group containing 15 rats. The survival of all groups and pathological changes of multiple organs (liver, kidney and lung) were observed at different time points after the operation. The pathologicalscore of multiple organs was carried out, followed by the determination of amylase, endotoxin and TNF-α contents in blood. The tissue microarray was used to detect the expression levels of NF-κB p65 protein in multiple organs. RESULTS: There was no marked difference between the model group and treatment group in the survival rate. The amylase content of the treatment group was significantly lower compared to the model group at 12 h (P < 0.01, 7791.00 vs 9195.00). Moreover, the endotoxin and TNF-α levels of the treatment group were significantly lower than that of the model group at 6 h and 12 h (P < 0.01, 0.040 vs 0.055, 0.042 vs 0.059 and P < 0.05, 58.30 vs 77.54, 38.70 vs 67.30, respectively). Regarding the changes in liver NF-κB expression, the model group significantly exceeded the sham operation group at 3 h (P < 0.01, 1.00 vs 0.00), and the treatment group significantly exceeded the sham operation group at 12 h (P < 0.01, 1.00 vs 0.00), whereas no marked difference was observed between the model group and treatment group at all time points. The kidney NF-κB expression level in the treatment group significantly exceeded the model group (P < 0.05, 2.00 vs 0.00) and the sham operation group (P < 0.01, 2.00 vs 0.00) at 12 h. No NF-κB expression in the lung was found in any group. CONCLUSION: Dexamethasone can lower the amylase, endotoxin and TNF-α levels as well as mortality of SAP rats. NF-κB plays an important role in multiple organ injury. Further studies should be conducted to determine whether dexamethasone can ameliorate the pathological changes of multiple organs by reducing the NF-κB expression in the liver and kidney. The advantages of tissue microarrays in pancreatitis pathological examination include time- and energy- saving, and are highly efficient and representative. The restriction of tissue microarrays on the representation of tissues to various extents due to small diameter may lead to the deviation of analysis.
基金supported by the National Natural Science Foundation of China (Grant Nos. 11790301, 11790305, 11973057, 12003048, 11903055, 11773043 and 12003049)supported by the Ministry of Science and Technology of China Key Project (2018YFA0404602)the international collaboration of ISSI-BJ
文摘The solar radio signal that can be received by the ground-based telescopes covers a wide frequency range,allowing us to monitor the complex physical processes occurred from the solar surface to the vast interplanetary space.MingantU SpEctral Radioheliograph(MUSER),as the latest generation of solar dedicated radio spectral-imaging instrument in the centimeter-decimeter wavelengths,has accumulated a large number of observational data since its commissioning observation in 2014.This paper presents the main observational results identified by MUSER from 2014 to 2019,including the quiet Sun and 94 solar radio burst events.We find that there are 81 events accompanied with Geostationary Operational Environmental Satellites(GOES)soft X-ray(SXR)flares,among which the smallest flare class is B1.0.There are 13 events without accompanying any recorded flares,among which the smallest SXR intensity during the radio burst period is equivalent to level-A.The main characteristics of all radio burst events are presented,which shows the powerful ability of MUSER to capture the valuable information of the solar non-thermal processes and the importance for space weather.This work also provides a database for further in-depth research.
基金funded by the National Natural Science Foundation of China(NSFC,Nos.11573043,11790305,11433006)National Key R&D 278 Program of China(2018YFA0404602)+1 种基金the CE4 mission of the Chinese Lunar Exploration Program:the Netherlands-China Low Frequency Explorer(NCLE)Chinese Academy of Sciences(CAS)Strategic Priority Research Program(XDA15020200)。
文摘The very low frequency(VLF)regime below 30 MHz in the electromagnetic spectrum has presently been drawing global attention in radio astronomical research due to its potentially significant science outcomes exploring many unknown extragalactic sources,transients,and so on.However,the nontransparency of the Earth’s ionosphere,ionospheric distortion and artificial radio frequency interference(RFI)have made it difficult to detect the VLF celestial radio emission with ground-based instruments.A straightforward solution to overcome these problems is a space-based VLF radio telescope,just like the VLF radio instruments onboard the Chang’E-4 spacecraft.But building such a space telescope would be inevitably costly and technically challenging.The alternative approach would be then a ground-based VLF radio telescope.Particularly,in the period of post 2020 when the solar and terrestrial ionospheric activities are expected to be in a’calm’state,it will provide us a good chance to perform VLF ground-based radio observations.Anticipating such an opportunity,we built an agile VLF radio spectrum explorer co-located with the currently operational Mingantu Spectra Radio Heliograph(MUSER).The instrument includes four antennas operating in the VLF frequency range 1-70 MHz.Along with them,we employ an eight-channel analog and digital receivers to amplify,digitize and process the radio signals received by the antennas.We present in the paper this VLF radio spectrum explorer and the instrument will be useful for celestial studies of VLF radio emissions.
