A special recurrent neural network(RNN),that is the zeroing neural network(ZNN),is adopted to find solutions to time-varying quadratic programming(TVQP)problems with equality and inequality constraints.Howevet,there a...A special recurrent neural network(RNN),that is the zeroing neural network(ZNN),is adopted to find solutions to time-varying quadratic programming(TVQP)problems with equality and inequality constraints.Howevet,there are some weaknesses in activation functions of traditional ZNN models,including convex restriction and redundant formulation.With the aid of different activation functions,modified ZNN models are obtained to overcome the drawbacks for solving TVQP problems.Theoretical and experimental research indicate that the proposed models are better and more effective at solving such TVQP problems.展开更多
Angle rigid multi-agent formations can simultaneously undergo translational,rotational,and scaling maneuvering,therefore combining the maneuvering capabilities of both distance and bearing rigid formations.However,man...Angle rigid multi-agent formations can simultaneously undergo translational,rotational,and scaling maneuvering,therefore combining the maneuvering capabilities of both distance and bearing rigid formations.However,maneuvering angle rigid formations in 2D or 3D with global convergence guarantees is shown to be a challenging problem in the existing literature even when relative position measurements are available.Motivated by angle-induced linear equations in 2D triangles and 3D tetrahedra,this paper aims to solve this challenging problem in both 2D and3D under a leader-follower framework.For the 2D case where the leaders have constant velocities,by using local relative position and velocity measurements,a formation maneuvering law is designed for the followers governed by double-integrator dynamics.When the leaders have time-varying velocities,a sliding mode formation maneuvering law is proposed by using the same measurements.For the 3D case,to establish an angle-induced linear equation for each tetrahedron,we assume that all the followers'coordinate frames share a common Z direction.Then,a formation maneuvering law is proposed for the followers to globally maneuver Z-weakly angle rigid formations in 3D.The extension to Lagrangian agent dynamics and the construction of the desired rigid formations by using the minimum number of angle constraints are also discussed.Simulation examples are provided to validate the effectiveness of the proposed algorithms.展开更多
N6-Methyladenosine(m^(6)A)is one of the most abundant modifications of eukaryotic mRNA,but its comprehensive biological functionality remains further exploration.In this study,we identified and characterized a new flo...N6-Methyladenosine(m^(6)A)is one of the most abundant modifications of eukaryotic mRNA,but its comprehensive biological functionality remains further exploration.In this study,we identified and characterized a new flowering-promoting gene,EARLY HEADING DATE6(EHD6),in rice.EHD6 encodes an RNA recognition motif(RRM)-containing RNA binding protein that is localized in the non-membranous cytoplasm ribonucleoprotein(RNP)granules and can bind both m^(6)A-modified RNA and unmodified RNA indiscriminately.We found that EHD6 can physically interact with YTH07,a YTH(YT521-B homology)domain-containing m^(6)A reader.We showed that their interaction enhances the binding of an m^(6)A-modified RNA and triggers relocation of a portion of YTH07 from the cytoplasm into RNP granules through phase-separated condensation.Within these condensates,the mRNA of a rice flowering repressor,CONSTANS-like 4(OsCOL4),becomes sequestered,leading to a reduction in its protein abundance and thus accelerated flowering through the Early heading date 1 pathway.Taken together,these results not only shed new light on the molecular mechanism of efficient m^(6)A recognition by the collaboration between an RNA binding protein and YTH family m^(6)A reader,but also uncover the potential for m^(6)A-mediated translation regulation through phaseseparated ribonucleoprotein condensation in rice.展开更多
This paper investigates the problem of Spacecraft Formation-Containment Flying Control(SFCFC)when the desired translational velocity is time-varying.In SFCFC problem,there are multiple leader spacecraft and multiple f...This paper investigates the problem of Spacecraft Formation-Containment Flying Control(SFCFC)when the desired translational velocity is time-varying.In SFCFC problem,there are multiple leader spacecraft and multiple follower spacecraft and SFCFC can be divided into leader spacecraft’s formation control and follower spacecraft’s containment control.First,under the condition that only a part of leader spacecraft can have access to the desired time-varying translational velocity,a velocity estimator is designed for each leader spacecraft.Secondly,based on the estimated translational velocity,a distributed formation control algorithm is designed for leader spacecraft to achieve the desired formation and move with the desired translational velocity simultaneously.Then,to ensure all follower spacecraft converge to the convex hull formed by the leader spacecraft,a distributed containment control algorithm is designed for follower spacecraft.Moreover,to reduce the dependence of the designed control algorithms on the graph information and increase system robustness,the control gains are changing adaptively and the parametric uncertainties are handled,respectively.Finally,simulation results are provided to illustrate the effectiveness of the theoretical results.展开更多
Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of plants.Previous studies have established that te...Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of plants.Previous studies have established that tetrapyrrole biosynthesis(TPB)and plastid gene expression(PGE)play essential roles in plastid retrograde signaling during early chloroplast biogenesis;however,their functional relationship remains unknown.In this study,we generated a series of rice TPB-related gun(genome uncoupled)mutants and systematically analyzed their effects on nuclear and plastid gene expression under normal conditions or when subjected to treatments with norflurazon(NF;a noncompetitive inhibitor of carotenoid biosynthesis)and/or lincomycin(Lin;a specific inhibitor of plastid translation).We show that under NF treatment,expression of plastid-encoded polymerase(PEP)-transcribed genes is significantly reduced in the wild type but is derepressed in the TPB-related gun mutants.We further demonstrate that the derepressed expression of PEPtranscribed genes may be caused by increased expression of the PEP core subunit and nuclear-encoded sigma factors and by elevated copy numbers of plastid genome per haploid genome.In addition,we show that expression of photosynthesis-associated nuclear genes(PhANGs)and PEP-transcribed genes is correlated in the rice TPB-related gun mutants,with or without NF or Lin treatment.A similar correlation between PhANGs and PGE is also observed in the Arabidopsis gun4 and gun5 mutants.Moreover,we show that increased expression of PEP-transcribed plastid genes is necessary for the gun phenotype in NF-treated TPB-related gun mutants.Further,we provide evidence that these TPB-related GUN genes act upstream of GUN1 in the regulation of retrograde signaling.Taken together,our results suggest that the TPB-related GUN genes control retrograde plastid signaling by regulating the PGE-dependent retrograde signaling pathway.展开更多
The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size （e.g., length and width） when breeding novel rice varie...The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size （e.g., length and width） when breeding novel rice varieties. It is challenging to measure such traits on a large scale in the field, and little is known about the genetic factors that determine the size of the top three leaves. In the present study, a population of recombinant inbred lines （RILs） and reciprocal single chromosomal segment substitution lines （SSSLs） derived from the progeny of a japonica Asominori x indica IR24 cross were grown under four diverse environmental conditions. Six morphological traits associated with leaf size were measured,namely length and width of the flag, second and third leaves. In the RIL population, 49 QTLs were identified that clustered in 30 genomic region. Twenty-three of these QTLs were confirmed in the SSSL population. A comparison with previously reported genes/QTLs revealed eight novel genomic regions that contained uncharacterized ORFs associated with leaf size. The QTLs identified in this study can be used for marker- assisted breeding and for fine mapping of novel genetic elements controlling leaf size in rice.展开更多
基金National Key Research and Development Programof China,Grant/Award Number:2017YFE0118900HHuawci Mindspore Academic Award Fund ofChinese Association of Artificial Intelligence,Grant/Award Number:CAALXSJLJ-2020-009A+5 种基金NaturalScience Foundation of Qinghai Province,China,Grant/Awad Number:2020-ZJ-903NaturalScience Foundation of Gansu Province,China,Grant/Award Number:20JR10RA639NaturalScience Foundation of Chongqing(China),Grant/Award Nurnber:cstc2020jcyj-zdxmX0028Researchand Development Foundation of Nanchong(China),Grant/Award Number:20YFZJ0018ChongqingKey Laboratory of Mobile CornrnunicationsTechnology,Grant/Award Nunber:cqupt-mct-202004Fundamental Research Funds for the Central Universities,Grant/Award Nurrber:lzujbky-2019-89.
文摘A special recurrent neural network(RNN),that is the zeroing neural network(ZNN),is adopted to find solutions to time-varying quadratic programming(TVQP)problems with equality and inequality constraints.Howevet,there are some weaknesses in activation functions of traditional ZNN models,including convex restriction and redundant formulation.With the aid of different activation functions,modified ZNN models are obtained to overcome the drawbacks for solving TVQP problems.Theoretical and experimental research indicate that the proposed models are better and more effective at solving such TVQP problems.
基金supported by National Natural Science Foundation of China(62173118)supported by the Ramon y Cajal(RYC2020-030090-I)from the Spanish Ministry of Science。
文摘Angle rigid multi-agent formations can simultaneously undergo translational,rotational,and scaling maneuvering,therefore combining the maneuvering capabilities of both distance and bearing rigid formations.However,maneuvering angle rigid formations in 2D or 3D with global convergence guarantees is shown to be a challenging problem in the existing literature even when relative position measurements are available.Motivated by angle-induced linear equations in 2D triangles and 3D tetrahedra,this paper aims to solve this challenging problem in both 2D and3D under a leader-follower framework.For the 2D case where the leaders have constant velocities,by using local relative position and velocity measurements,a formation maneuvering law is designed for the followers governed by double-integrator dynamics.When the leaders have time-varying velocities,a sliding mode formation maneuvering law is proposed by using the same measurements.For the 3D case,to establish an angle-induced linear equation for each tetrahedron,we assume that all the followers'coordinate frames share a common Z direction.Then,a formation maneuvering law is proposed for the followers to globally maneuver Z-weakly angle rigid formations in 3D.The extension to Lagrangian agent dynamics and the construction of the desired rigid formations by using the minimum number of angle constraints are also discussed.Simulation examples are provided to validate the effectiveness of the proposed algorithms.
基金supported by the Key Laboratory of Biology,Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River,Ministry of Agriculture and Rural Affairs,China,and the Jiangsu Collaborative Innovation Center for Modern Crop Production,China.Funding for this work was provided by the National Key Research and Development Program of China(2020YFE0202300 and 2021YFD1200504)the National Natural Science Foundation of China(31971910 and 32272115)+1 种基金the National Science Foundation of Jiangsu Province(BK20212010 and BK20230038)the Foundation of Biological Breeding Zhongshan Lab(BM2022008-03,ZSBBL-KY2023-04,and ZSBBL-KY2023-06).
文摘N6-Methyladenosine(m^(6)A)is one of the most abundant modifications of eukaryotic mRNA,but its comprehensive biological functionality remains further exploration.In this study,we identified and characterized a new flowering-promoting gene,EARLY HEADING DATE6(EHD6),in rice.EHD6 encodes an RNA recognition motif(RRM)-containing RNA binding protein that is localized in the non-membranous cytoplasm ribonucleoprotein(RNP)granules and can bind both m^(6)A-modified RNA and unmodified RNA indiscriminately.We found that EHD6 can physically interact with YTH07,a YTH(YT521-B homology)domain-containing m^(6)A reader.We showed that their interaction enhances the binding of an m^(6)A-modified RNA and triggers relocation of a portion of YTH07 from the cytoplasm into RNP granules through phase-separated condensation.Within these condensates,the mRNA of a rice flowering repressor,CONSTANS-like 4(OsCOL4),becomes sequestered,leading to a reduction in its protein abundance and thus accelerated flowering through the Early heading date 1 pathway.Taken together,these results not only shed new light on the molecular mechanism of efficient m^(6)A recognition by the collaboration between an RNA binding protein and YTH family m^(6)A reader,but also uncover the potential for m^(6)A-mediated translation regulation through phaseseparated ribonucleoprotein condensation in rice.
基金supported by the National Natural Science Foundation of China(Nos.61876050,61673135,61603114).
文摘This paper investigates the problem of Spacecraft Formation-Containment Flying Control(SFCFC)when the desired translational velocity is time-varying.In SFCFC problem,there are multiple leader spacecraft and multiple follower spacecraft and SFCFC can be divided into leader spacecraft’s formation control and follower spacecraft’s containment control.First,under the condition that only a part of leader spacecraft can have access to the desired time-varying translational velocity,a velocity estimator is designed for each leader spacecraft.Secondly,based on the estimated translational velocity,a distributed formation control algorithm is designed for leader spacecraft to achieve the desired formation and move with the desired translational velocity simultaneously.Then,to ensure all follower spacecraft converge to the convex hull formed by the leader spacecraft,a distributed containment control algorithm is designed for follower spacecraft.Moreover,to reduce the dependence of the designed control algorithms on the graph information and increase system robustness,the control gains are changing adaptively and the parametric uncertainties are handled,respectively.Finally,simulation results are provided to illustrate the effectiveness of the theoretical results.
基金supported by grants from the National Natural Science Foundation of China(91935301)National Natural Science Foundation of China Joint Program(U1701232)+4 种基金Jiangsu Science and Technology Development Program(BE2021360)Jiangsu Agricultural Science and Technology Innovation Fund Project(SCX(19)1079)Jiangsu Province Agriculture Independent Innovation Fund Project(CX(19)1002)National Key Research and Development Program of China(2016YFD0100903)the Fundamental Research Funds for the Central Universities(JCQY201902).
文摘Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of plants.Previous studies have established that tetrapyrrole biosynthesis(TPB)and plastid gene expression(PGE)play essential roles in plastid retrograde signaling during early chloroplast biogenesis;however,their functional relationship remains unknown.In this study,we generated a series of rice TPB-related gun(genome uncoupled)mutants and systematically analyzed their effects on nuclear and plastid gene expression under normal conditions or when subjected to treatments with norflurazon(NF;a noncompetitive inhibitor of carotenoid biosynthesis)and/or lincomycin(Lin;a specific inhibitor of plastid translation).We show that under NF treatment,expression of plastid-encoded polymerase(PEP)-transcribed genes is significantly reduced in the wild type but is derepressed in the TPB-related gun mutants.We further demonstrate that the derepressed expression of PEPtranscribed genes may be caused by increased expression of the PEP core subunit and nuclear-encoded sigma factors and by elevated copy numbers of plastid genome per haploid genome.In addition,we show that expression of photosynthesis-associated nuclear genes(PhANGs)and PEP-transcribed genes is correlated in the rice TPB-related gun mutants,with or without NF or Lin treatment.A similar correlation between PhANGs and PGE is also observed in the Arabidopsis gun4 and gun5 mutants.Moreover,we show that increased expression of PEP-transcribed plastid genes is necessary for the gun phenotype in NF-treated TPB-related gun mutants.Further,we provide evidence that these TPB-related GUN genes act upstream of GUN1 in the regulation of retrograde signaling.Taken together,our results suggest that the TPB-related GUN genes control retrograde plastid signaling by regulating the PGE-dependent retrograde signaling pathway.
基金supported by the National Natural Science Foundation of China(31471174,and 31271798)
文摘The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size （e.g., length and width） when breeding novel rice varieties. It is challenging to measure such traits on a large scale in the field, and little is known about the genetic factors that determine the size of the top three leaves. In the present study, a population of recombinant inbred lines （RILs） and reciprocal single chromosomal segment substitution lines （SSSLs） derived from the progeny of a japonica Asominori x indica IR24 cross were grown under four diverse environmental conditions. Six morphological traits associated with leaf size were measured,namely length and width of the flag, second and third leaves. In the RIL population, 49 QTLs were identified that clustered in 30 genomic region. Twenty-three of these QTLs were confirmed in the SSSL population. A comparison with previously reported genes/QTLs revealed eight novel genomic regions that contained uncharacterized ORFs associated with leaf size. The QTLs identified in this study can be used for marker- assisted breeding and for fine mapping of novel genetic elements controlling leaf size in rice.
