Background:Shear stress-induced erythrocyte damage,namely hemolysis,is an important problem in the development of blood-contacting medical devices such as mechanical circulatory support devices.Computational fluid dyn...Background:Shear stress-induced erythrocyte damage,namely hemolysis,is an important problem in the development of blood-contacting medical devices such as mechanical circulatory support devices.Computational fluid dynamics simulation combined with hemolysis prediction models have been widely used to predict hemolysis.With the development of hemolysis prediction models,the new hemolysis prediction model requires more experimental data to verify.In addition,the difference of in vitro blood-shearing device also affect the accuracy of hemolysis prediction.Methods:To address these problems,a new in vitro blood-shearing device(vortex oscillator)was used to further verify the accuracy of the hemolysis prediction models,and to guide the optimal design of blood-contacting medical devices such as mechanical circulatory support devices.Firstly,the flow field information such as wall stress and velocity of the vortex oscillator under different speeds was analyzed.Secondly,different hemolysis prediction models were used to calculate hemolysis,and the predicted data was compared with the experimental data.Results and Conclusion:In this study,the flow field information inside the vortex oscillator at high rotational speeds was systematically investigated,and the prediction of hemolysis was carried out.The results showed that the predicted data of hemolysis was significantly different from the experimental data,which indicated that it was urgent to establish a standardized in vitro blood-shearing platform to provide a reference for accurate hemolysis prediction.展开更多
Enhancing the water permeation while maintaining high salt rejection of existing reverse osmosis(RO)membranes remains a considerable challenge.Herein,we proposed to introduce polymer of intrinsic microporosity,PIM-1,i...Enhancing the water permeation while maintaining high salt rejection of existing reverse osmosis(RO)membranes remains a considerable challenge.Herein,we proposed to introduce polymer of intrinsic microporosity,PIM-1,into the selective layer of reverse osmosis membranes to break the trade-off effect between permeability and selectivity.A water-soluble a-LPIM-1 of low-molecular-weight and hydroxyl terminals was synthesized.These designed characteristics endowed it with high solubility and reactivity.Then it was mixed with m-phenylenediamine and together served as aqueous monomer to react with organic monomer of trimesoyl chloride via interfacial polymerization.The characterization results exhibited that more“nodule”rather than“leaf”structure formed on RO membrane surface,which indicated that the introduction of the high free-volume of a-LPIM-1 with three dimensional twisted and folded structure into the selective layer effectively caused the frustrated packing between polymer chains.In virtue of this effect,even with reduced surface roughness and unchanged layer thickness,the water permeability of prepared reverse osmosis membranes increased 2.1 times to 62.8 L·m^(-2)·h^(-1) with acceptable Na Cl rejection of 97.6%.This attempt developed a new strategy to break the trade-off effect faced by traditional polyamide reverse osmosis membranes.展开更多
With the increasing number of people suffering from heart failure,ventricular assist devices have gradually become an effective way to treat end-stage heart failure.However,the blood damage caused by ventricular assis...With the increasing number of people suffering from heart failure,ventricular assist devices have gradually become an effective way to treat end-stage heart failure.However,the blood damage caused by ventricular assist devices has not been effectively solved,which is an obstacle to its clinical promotion.Most research focused on erythrocyte damage under shear stress,while few researches were conducted on the interaction between blood under shear stress and the induction of von Willebrand factor(VWF)damage.This research used a vortex oscillator blood-shearing platform to conduct in vitro experiments and used immunoblotting to quantify VWF damage in sheared samples to study the laws of shear-induced VWF damage under different shear stress,different exposure times,different blood components,and hemolysis conditions.It was found that VWF damage increased with exposure time and shear stress.At the same time,under lower shear stress,other blood components had little effect on VWF damage,while in a higher shear stress,other blood components would accelerate VWF damage.Hemolysis will also affect VWF damage,and the higher the degree of hemolysis,the higher the rate of VWF degradation in the plasma.The results of this research provide a reference for VWF damage evaluation standards and follow-up research and also guide for improving the design of ventricular assist devices to reduce VWF damage.展开更多
基金supported by 2023 Kunshan Science and Technology Association youth science and technology talent lifting project(Project name:Mechanism study of mechanical damage of coagulation factor VWF based on in vitro blood-shearing experimental platform).
文摘Background:Shear stress-induced erythrocyte damage,namely hemolysis,is an important problem in the development of blood-contacting medical devices such as mechanical circulatory support devices.Computational fluid dynamics simulation combined with hemolysis prediction models have been widely used to predict hemolysis.With the development of hemolysis prediction models,the new hemolysis prediction model requires more experimental data to verify.In addition,the difference of in vitro blood-shearing device also affect the accuracy of hemolysis prediction.Methods:To address these problems,a new in vitro blood-shearing device(vortex oscillator)was used to further verify the accuracy of the hemolysis prediction models,and to guide the optimal design of blood-contacting medical devices such as mechanical circulatory support devices.Firstly,the flow field information such as wall stress and velocity of the vortex oscillator under different speeds was analyzed.Secondly,different hemolysis prediction models were used to calculate hemolysis,and the predicted data was compared with the experimental data.Results and Conclusion:In this study,the flow field information inside the vortex oscillator at high rotational speeds was systematically investigated,and the prediction of hemolysis was carried out.The results showed that the predicted data of hemolysis was significantly different from the experimental data,which indicated that it was urgent to establish a standardized in vitro blood-shearing platform to provide a reference for accurate hemolysis prediction.
基金supported by Zhejiang Provincial Natural Science Foundation of China (LZ20B060001)National Natural Science Foundation of China (22008208&21908192)China Postdoctoral Science Foundation (2019TQ0276)。
文摘Enhancing the water permeation while maintaining high salt rejection of existing reverse osmosis(RO)membranes remains a considerable challenge.Herein,we proposed to introduce polymer of intrinsic microporosity,PIM-1,into the selective layer of reverse osmosis membranes to break the trade-off effect between permeability and selectivity.A water-soluble a-LPIM-1 of low-molecular-weight and hydroxyl terminals was synthesized.These designed characteristics endowed it with high solubility and reactivity.Then it was mixed with m-phenylenediamine and together served as aqueous monomer to react with organic monomer of trimesoyl chloride via interfacial polymerization.The characterization results exhibited that more“nodule”rather than“leaf”structure formed on RO membrane surface,which indicated that the introduction of the high free-volume of a-LPIM-1 with three dimensional twisted and folded structure into the selective layer effectively caused the frustrated packing between polymer chains.In virtue of this effect,even with reduced surface roughness and unchanged layer thickness,the water permeability of prepared reverse osmosis membranes increased 2.1 times to 62.8 L·m^(-2)·h^(-1) with acceptable Na Cl rejection of 97.6%.This attempt developed a new strategy to break the trade-off effect faced by traditional polyamide reverse osmosis membranes.
基金supported by 2023 Kunshan Science and Technology Association youth science and technology talent lifting project(Project name:Mechanism study of mechanical damage of coagulation factor VWF based on in vitro blood-shearing experimental platform).
文摘With the increasing number of people suffering from heart failure,ventricular assist devices have gradually become an effective way to treat end-stage heart failure.However,the blood damage caused by ventricular assist devices has not been effectively solved,which is an obstacle to its clinical promotion.Most research focused on erythrocyte damage under shear stress,while few researches were conducted on the interaction between blood under shear stress and the induction of von Willebrand factor(VWF)damage.This research used a vortex oscillator blood-shearing platform to conduct in vitro experiments and used immunoblotting to quantify VWF damage in sheared samples to study the laws of shear-induced VWF damage under different shear stress,different exposure times,different blood components,and hemolysis conditions.It was found that VWF damage increased with exposure time and shear stress.At the same time,under lower shear stress,other blood components had little effect on VWF damage,while in a higher shear stress,other blood components would accelerate VWF damage.Hemolysis will also affect VWF damage,and the higher the degree of hemolysis,the higher the rate of VWF degradation in the plasma.The results of this research provide a reference for VWF damage evaluation standards and follow-up research and also guide for improving the design of ventricular assist devices to reduce VWF damage.
