There exists large space to save energy of high-sulfur natural gas purification process.The multi-objective optimization problem has been investigated to effectively reduce the total comprehensive energy consumption a...There exists large space to save energy of high-sulfur natural gas purification process.The multi-objective optimization problem has been investigated to effectively reduce the total comprehensive energy consumption and further improve the production rate of purified gas.A steady-state simulation model of high-sulfur natural gas purification process has been set up by using ProMax.Seven key operating parameters of the purification process have been determined based on the analysis of comprehensive energy consumption distribution.To solve the problem that the process model does not converge in some conditions,back-propagation(BP)neural network has been applied to substitute the simulation model to predict the relative parameters in the optimization model.The uniform design method and the table U21(107)have been applied to design the experiment points for training and testing BP model.High prediction accuracy can be achieved by using the BP model.Nondominated sorting genetic algorithm-II has been developed to optimize the two objectives,and 100 Pareto optimal solutions have been obtained.Three optimal points have been selected and evaluated further.The results demonstrate that the total comprehensive energy consumption is reduced by 13.4%and the production rate of purified gas is improved by 0.2%under the optimized operating conditions.展开更多
The clinical application of cancer immunotherapy is unsatisfied due to low response rates and systemic immune-related adverse events.Microwave hyperthermia can be used as a synergistic immunotherapy to amplify the ant...The clinical application of cancer immunotherapy is unsatisfied due to low response rates and systemic immune-related adverse events.Microwave hyperthermia can be used as a synergistic immunotherapy to amplify the antitumor effect.Herein,we designed a Gd-based metal-organic framework(Gd-MOF)nanosystem for MRI-guided thermotherapy and synergistic immunotherapy,which featured high performance in drug loading and tumor tissue penetration.The PD-1 inhibitor(aPD-1)was initially loaded in the porous Gd-MOF(Gd/M)nanosystem.Then,the phase change material(PCM)and the cancer cell membrane were further sequentially modified on the surface of Gd/MP to obtain Gd-MOF@aPD-1@CM(Gd/MPC).When entering the tumor microenvironment(TME),Gd/MPC induces immunogenic death of tumor cells through microwave thermal responsiveness,improves tumor suppressive immune microenvironment and further enhances anti-tumor ability of T cells by releasing aPD-1.Meanwhile,Gd/MPC can be used for contrast-enhanced MRI.Transcriptomics data revealed that the downregulation of MSK2 in cancer cells leads to the downregulation of c-fos and c-jun,and ultimately leads to the apoptosis of cancer cells after treatment.In general,Gd/MPC nanosystem not only solves the problem of system side effect,but also achieves the controlled drug release via PCM,providing a promising theranostic nanoplatform for development of cancer combination immunotherapy.展开更多
Rapid development of checkpoint inhibitors has provided significant breakthroughs for cancer stem cell(CSC)therapy,while the therapeutic efficacy is restricted by hypoxia-mediated tumor immune evasion,especially hypox...Rapid development of checkpoint inhibitors has provided significant breakthroughs for cancer stem cell(CSC)therapy,while the therapeutic efficacy is restricted by hypoxia-mediated tumor immune evasion,especially hypoxia-induced CD47 overexpression in CSCs.Herein,we developed a genetically engineered CSC membrane-coated hollow manganese dioxide(hMnO_(2)@gCMs)to elicit robust antitumor immunity by blocking CD47 and alleviating hypoxia to ultimately achieve the eradication of CSCs.The hMnO_(2)core effectively alleviated tumor hypoxia by inducing decomposition of tumor endogenous H_(2)O_(2),thus suppressing the CSCs and reducing the expression of CD47.Cooperating with hypoxia relief-induced downregulation of CD47,the overexpressed SIRPαon gCM shell efficiently blocked the CD47-SIRPα“don’t eat me”pathway,synergistically eliciting robust antitumor-mediated immune responses.In a B16F10-CSC bearing melanoma mouse model,the hMnO_(2)@gCMs showed an enhanced therapeutic effect in eradicating CSCs and inhibiting tumor growth.Our work presents a simple,safe,and robust platform for CSC eradication and cancer immunotherapy.展开更多
4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole（NBD-F） was employed as a colorimetric probe for differential detection of biothiols in different media. The spectral response and the selectivity of NBD-F toward thiols were s...4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole（NBD-F） was employed as a colorimetric probe for differential detection of biothiols in different media. The spectral response and the selectivity of NBD-F toward thiols were significantly improved by surfactant micelles. Mercapto group exhibited high reactivity in all the solvents（including Tris–HCl buffer solution, CTAB and SDS micelles）. The 4-thioether derivatives of NBDF reacting with Cys and Hcy but not GSH could transfer to the corresponding 4-amino-substituents via intramolecular nucleophilic aromatic substitution, thus, GSH could be discriminated from Cys/Hcy. In CTAB micelles, the reaction product of NBD-F with Cys is non-fluorescent and it absorbs in longwavelength region. According to the spectral responses of NBD-F toward different low-molecularweight thiols, we could identify Cys, Hcy and GSH from each other.展开更多
基金Financial support from National Science and Technology Major Project of the Ministry of Science and Technology of China(Grant No.2016ZX05017-004)
文摘There exists large space to save energy of high-sulfur natural gas purification process.The multi-objective optimization problem has been investigated to effectively reduce the total comprehensive energy consumption and further improve the production rate of purified gas.A steady-state simulation model of high-sulfur natural gas purification process has been set up by using ProMax.Seven key operating parameters of the purification process have been determined based on the analysis of comprehensive energy consumption distribution.To solve the problem that the process model does not converge in some conditions,back-propagation(BP)neural network has been applied to substitute the simulation model to predict the relative parameters in the optimization model.The uniform design method and the table U21(107)have been applied to design the experiment points for training and testing BP model.High prediction accuracy can be achieved by using the BP model.Nondominated sorting genetic algorithm-II has been developed to optimize the two objectives,and 100 Pareto optimal solutions have been obtained.Three optimal points have been selected and evaluated further.The results demonstrate that the total comprehensive energy consumption is reduced by 13.4%and the production rate of purified gas is improved by 0.2%under the optimized operating conditions.
基金support from National Natural Science Foundation of China[82103404]Southern Medical University Excellent Youth Scholars Training Program[2020YQPY008]+3 种基金Stomatological Hospital of Southern Medical University Startup Funds[No.PY2020001,PY2019026]Natural Science Foundation of Guang dong Province(2023A1515011604)The Fundamental Research Funds for the Central Universities(2022ZYGXZR042)the High-level Hospital Construction Project(KJ012019100)for financial support.
文摘The clinical application of cancer immunotherapy is unsatisfied due to low response rates and systemic immune-related adverse events.Microwave hyperthermia can be used as a synergistic immunotherapy to amplify the antitumor effect.Herein,we designed a Gd-based metal-organic framework(Gd-MOF)nanosystem for MRI-guided thermotherapy and synergistic immunotherapy,which featured high performance in drug loading and tumor tissue penetration.The PD-1 inhibitor(aPD-1)was initially loaded in the porous Gd-MOF(Gd/M)nanosystem.Then,the phase change material(PCM)and the cancer cell membrane were further sequentially modified on the surface of Gd/MP to obtain Gd-MOF@aPD-1@CM(Gd/MPC).When entering the tumor microenvironment(TME),Gd/MPC induces immunogenic death of tumor cells through microwave thermal responsiveness,improves tumor suppressive immune microenvironment and further enhances anti-tumor ability of T cells by releasing aPD-1.Meanwhile,Gd/MPC can be used for contrast-enhanced MRI.Transcriptomics data revealed that the downregulation of MSK2 in cancer cells leads to the downregulation of c-fos and c-jun,and ultimately leads to the apoptosis of cancer cells after treatment.In general,Gd/MPC nanosystem not only solves the problem of system side effect,but also achieves the controlled drug release via PCM,providing a promising theranostic nanoplatform for development of cancer combination immunotherapy.
基金supported by the National Natural Science Foundation of China(Nos.82222035 and 82372106)the Guangdong Basic and Applied Basic Research Foundation(No.2023A1515110633)+2 种基金the Shenzhen Medical Research Found(No.B2302041)the Shenzhen Science and Technology Program(No.RCBS20221008093123060)the Shenzhen Bay Laboratory Proof-of-Concept Fund(No.S231801005).
文摘Rapid development of checkpoint inhibitors has provided significant breakthroughs for cancer stem cell(CSC)therapy,while the therapeutic efficacy is restricted by hypoxia-mediated tumor immune evasion,especially hypoxia-induced CD47 overexpression in CSCs.Herein,we developed a genetically engineered CSC membrane-coated hollow manganese dioxide(hMnO_(2)@gCMs)to elicit robust antitumor immunity by blocking CD47 and alleviating hypoxia to ultimately achieve the eradication of CSCs.The hMnO_(2)core effectively alleviated tumor hypoxia by inducing decomposition of tumor endogenous H_(2)O_(2),thus suppressing the CSCs and reducing the expression of CD47.Cooperating with hypoxia relief-induced downregulation of CD47,the overexpressed SIRPαon gCM shell efficiently blocked the CD47-SIRPα“don’t eat me”pathway,synergistically eliciting robust antitumor-mediated immune responses.In a B16F10-CSC bearing melanoma mouse model,the hMnO_(2)@gCMs showed an enhanced therapeutic effect in eradicating CSCs and inhibiting tumor growth.Our work presents a simple,safe,and robust platform for CSC eradication and cancer immunotherapy.
基金financially supported by Shanghai Municipal Natural Science Foundation(No.15ZR1409000)the open fund of Shanghai Key Laboratory of Chemical Biology(No.SKLCB-2013-03)
文摘4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole（NBD-F） was employed as a colorimetric probe for differential detection of biothiols in different media. The spectral response and the selectivity of NBD-F toward thiols were significantly improved by surfactant micelles. Mercapto group exhibited high reactivity in all the solvents（including Tris–HCl buffer solution, CTAB and SDS micelles）. The 4-thioether derivatives of NBDF reacting with Cys and Hcy but not GSH could transfer to the corresponding 4-amino-substituents via intramolecular nucleophilic aromatic substitution, thus, GSH could be discriminated from Cys/Hcy. In CTAB micelles, the reaction product of NBD-F with Cys is non-fluorescent and it absorbs in longwavelength region. According to the spectral responses of NBD-F toward different low-molecularweight thiols, we could identify Cys, Hcy and GSH from each other.
