Neighborhood gardens serve as sensitive sites for human microbial encounters,with phyllosphere microbes directly impacting our respiratory health.Yet,our understanding remains limited on how factors like season,garden...Neighborhood gardens serve as sensitive sites for human microbial encounters,with phyllosphere microbes directly impacting our respiratory health.Yet,our understanding remains limited on how factors like season,garden age,and land use shape the risk of respiratory diseases(RDs)tied to these garden microbes.Here we examined the microbial communities within the phyllosphere of 72 neighborhood gardens across Shanghai,spanning different seasons(warm and cold),garden ages(old and young),and locales(urban and rural).We found a reduced microbial diversity during the cold season,except for Gammaproteobacteria which exhibited an inverse trend.While land use influenced the microbial composition,urban and rural gardens had strikingly similar microbial profiles.Alarmingly,young gardens in the cold season hosted a substantial proportion of RDs-associated species,pointing towards increased respiratory inflammation risks.In essence,while newer gardens during colder periods show a decline in microbial diversity,they have an increased presence of RDs-associated microbes,potentially escalating respiratory disease prevalence.This underscores the pivotal role the garden age plays in enhancing both urban microbial diversity and respiratory health.展开更多
Soils and ecosystems contaminated with cadmium (Cd) threaten human health and adversely affect morphological,physiological,and biochemical parameters of plants.The symbiotic association of endophytic fungi with their ...Soils and ecosystems contaminated with cadmium (Cd) threaten human health and adversely affect morphological,physiological,and biochemical parameters of plants.The symbiotic association of endophytic fungi with their host plants is the best strategy to improve various plant characteristics and remediate soils polluted with heavy metal(loid)s (HMs).Being a well-known plant growth-promoting fungus,Piriformospora indica confers resistance against a number of abiotic stresses,including HM stress.This pot experiment explored the potential and ameliorative effects of P.indica on Artemisia annua L.plants treated with different concentrations (0,40,80,and 120 mg kg-1) of Cd.Inoculation with P.indica significantly increased plant performance,especially by enhancing chlorophyll concentration and water potential and by decreasing electrolytic leakage,when compared with un-inoculated plants,despite the high Cd levels.Similarly,P.indica enhanced antioxidant enzyme activities,thereby reducing the drastic effects of Cd in inoculated plants.In addition,P.indica accumulated Cd in the roots of colonized plants,as revealed by atomic absorption spectroscopy,and restricted Cd translocation to aerial parts.Furthermore,P.indica showed in vitro resistance (up to a certain level) to Cd stress;however,fungus growth was inhibited at very high Cd concentrations,proving it an excellent candidate for use as a potential phytoremediator in fields affected by Cd contamination.The transcriptional analysis showed that the signaling genes and artemisinin and flavonoid biosynthetic pathway genes were significantly upregulated in P.indica-co-cultivated plants when compared with un-inoculated plants,suggesting a fine collaboration between primary and secondary metabolisms to modulate resistance capacity and to enhance the phytoremediation capability of A.annua against Cd toxicity.展开更多
基金supported by the Natural Science Foundation of China(Project number:32371843)the Science and Technology Commission of Shanghai Municipality(Project number:22230713300).
文摘Neighborhood gardens serve as sensitive sites for human microbial encounters,with phyllosphere microbes directly impacting our respiratory health.Yet,our understanding remains limited on how factors like season,garden age,and land use shape the risk of respiratory diseases(RDs)tied to these garden microbes.Here we examined the microbial communities within the phyllosphere of 72 neighborhood gardens across Shanghai,spanning different seasons(warm and cold),garden ages(old and young),and locales(urban and rural).We found a reduced microbial diversity during the cold season,except for Gammaproteobacteria which exhibited an inverse trend.While land use influenced the microbial composition,urban and rural gardens had strikingly similar microbial profiles.Alarmingly,young gardens in the cold season hosted a substantial proportion of RDs-associated species,pointing towards increased respiratory inflammation risks.In essence,while newer gardens during colder periods show a decline in microbial diversity,they have an increased presence of RDs-associated microbes,potentially escalating respiratory disease prevalence.This underscores the pivotal role the garden age plays in enhancing both urban microbial diversity and respiratory health.
基金the School of Agriculture and Biology, Shanghai Jiao Tong University, China for providing financial support and experimental facilities。
文摘Soils and ecosystems contaminated with cadmium (Cd) threaten human health and adversely affect morphological,physiological,and biochemical parameters of plants.The symbiotic association of endophytic fungi with their host plants is the best strategy to improve various plant characteristics and remediate soils polluted with heavy metal(loid)s (HMs).Being a well-known plant growth-promoting fungus,Piriformospora indica confers resistance against a number of abiotic stresses,including HM stress.This pot experiment explored the potential and ameliorative effects of P.indica on Artemisia annua L.plants treated with different concentrations (0,40,80,and 120 mg kg-1) of Cd.Inoculation with P.indica significantly increased plant performance,especially by enhancing chlorophyll concentration and water potential and by decreasing electrolytic leakage,when compared with un-inoculated plants,despite the high Cd levels.Similarly,P.indica enhanced antioxidant enzyme activities,thereby reducing the drastic effects of Cd in inoculated plants.In addition,P.indica accumulated Cd in the roots of colonized plants,as revealed by atomic absorption spectroscopy,and restricted Cd translocation to aerial parts.Furthermore,P.indica showed in vitro resistance (up to a certain level) to Cd stress;however,fungus growth was inhibited at very high Cd concentrations,proving it an excellent candidate for use as a potential phytoremediator in fields affected by Cd contamination.The transcriptional analysis showed that the signaling genes and artemisinin and flavonoid biosynthetic pathway genes were significantly upregulated in P.indica-co-cultivated plants when compared with un-inoculated plants,suggesting a fine collaboration between primary and secondary metabolisms to modulate resistance capacity and to enhance the phytoremediation capability of A.annua against Cd toxicity.
