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百合不同品种间花粉萌发活力检测分析 被引量:7
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作者 于金平 王媛媛 +3 位作者 张琪 王文和 冷平生 赵祥云 《沈阳农业大学学报》 CAS CSCD 北大核心 2018年第1期14-19,共6页
以不同类型百合的花粉为研究对象,探讨不同品种百合花粉萌发的特点以及不同类型百合花粉的萌发是否具有一定的规律性,旨在为百合杂交育种中父本材料的选择以及授粉方法的改良提供依据。以百合5个类型共43个品种的花粉为材料,利用离体萌... 以不同类型百合的花粉为研究对象,探讨不同品种百合花粉萌发的特点以及不同类型百合花粉的萌发是否具有一定的规律性,旨在为百合杂交育种中父本材料的选择以及授粉方法的改良提供依据。以百合5个类型共43个品种的花粉为材料,利用离体萌发法,探讨不同品种百合花粉萌发活力,筛选适用于大部分品种的最佳培养基,结果表明,在蔗糖100g·L^(-1)、硼酸20mg·L^(-1)、琼脂10g·L^(-1)的固体培养基上可以较好地萌发。大多数百合品种都能在12h内达到最大萌发率,也有少数品种在24h还处于萌发状态。百合会因自身遗传特性导致萌发率不同,其中,TR类百合总体萌发活力最强,平均萌发率80.98%;LA类百合萌发率最低,平均为13.49%;OT类、A类和AT类萌发率居中,分别为49.06%,46.05%和50.06%。百合花粉管自身寿命也存在明显差异,在43个百合品种中,发现有18个品种在培养后期花粉萌发率明显下降,这主要是由于培养后期花粉管自身破裂所导致的。因此,在进行杂交育种的过程中,可根据育种目的有针对性地选择父本材料,其中,TR类百合是非常好的父本材料;其他萌发率低于50%的资源做父本时可加大授粉量、采取二次授粉或切割花柱等方式提高授粉成功率;本试验中花粉不能萌发的资源建议不做父本应用。 展开更多
关键词 百合 离体培养条件 最适培养基 花粉萌发率
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水淹胁迫对4年生华北紫丁香生理指标的影响 被引量:11
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作者 白果 冷平生 胡增辉 《西部林业科学》 CAS 北大核心 2021年第4期101-106,共6页
为探索提高华北紫丁香耐涝性的途径和方法,筛选和培育耐涝性强的品系,以4 a生华北紫丁香成苗为材料,采用盆栽模拟水淹环境,经水淹0、1、4、7、10、16 d后,测定叶片含水量、相对电导率、丙二醛含量、可溶性糖含量、脯氨酸含量,以及过氧... 为探索提高华北紫丁香耐涝性的途径和方法,筛选和培育耐涝性强的品系,以4 a生华北紫丁香成苗为材料,采用盆栽模拟水淹环境,经水淹0、1、4、7、10、16 d后,测定叶片含水量、相对电导率、丙二醛含量、可溶性糖含量、脯氨酸含量,以及过氧化物酶、过氧化氢酶活性和叶绿素荧光等指标,分析其对水淹胁迫的生理响应。结果显示,华北紫丁香叶片相对含水量随水淹胁迫时间延长逐渐降低,在7 d时出现较明显下降,而相对电导率和丙二醛含量则逐渐升高,表明伤害程度越来越重;F_(o)和F_(m)呈现先升高后降低的趋势,而F_(v)/F_(m)和Yield则逐渐下降;可溶性糖含量、过氧化物酶和过氧化氢酶活性呈现出先升高后降低的趋势,在7 d时达到最高;脯氨酸含量则持续升高。总体来看,华北紫丁香在胁迫10 d时,伤害指标变化的加剧和抗性指标的明显降低说明已产生不可逆损伤。由此可见,4 a生丁香成苗表现出一定的耐涝性,可耐受近7 d的水淹胁迫。 展开更多
关键词 华北紫丁香 水淹胁迫 耐涝性 生理指标
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Characteristics of soil seed bank in plantation forest in the rocky mountain region of Beijing, China 被引量:5
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作者 HU Zeng-hui YANG Yang +3 位作者 leng ping-sheng DOU De-quan ZHANG Bo HOU Bing-fei 《Journal of Forestry Research》 SCIE CAS CSCD 2013年第1期91-97,共7页
We investigated characteristics (scales and composition) of soil seed banks at eight study sites in the rocky mountain region of Beijing by seed identification and germination monitoring. We also surveyed the vegeta... We investigated characteristics (scales and composition) of soil seed banks at eight study sites in the rocky mountain region of Beijing by seed identification and germination monitoring. We also surveyed the vegetation communities at the eight study sites to explore the role of soil seed banks in vegetation restoration. The storage capacity of soil seed banks at the eight sites ranked from 766.26 to 2461.92 seeds·m?2. A total of 23 plant species were found in soil seed banks, of which 63–80% of seeds were herbs in various soil layers and 60% of seeds were located in the soil layer at 0–5 cm depth. Biodiversity indices indicated clear differences in species diversity of soil seed banks among different plant communities. The species composition of aboveground vegetation showed low similarity with that based on soil seed banks. In the aboveground plant community, the afforestation tree species showed high importance values. The plant species originating from soil seed banks represented natural regeneration, which also showed relatively high importance values. This study suggests that in the rocky mountain region of Beijing the soil seed banks played a key role in the transformation from pure plantation forest to near-natural forest, promoting natural ecological processes, and the role of the seed banks in vegetation restoration was important to the improvement of ecological restoration methods. 展开更多
关键词 Rocky mountain region of Beijing soil seed bank plantcommunity plant diversity vegetation regeneration
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Emissions of saturated C6-C10 aldehydes from poplar (Populus simonii×P. pyramidalis ‘Opera 8277’) cuttings at different levels of light intensity 被引量:1
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作者 HU Zeng-hui leng ping-sheng +1 位作者 SHEN Ying-bai WANG Wen-he 《Journal of Forestry Research》 SCIE CAS CSCD 2011年第2期233-238,共6页
Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.py... Aldehydes play an important role in atmospheric chemistry and plant direct and indirect defense against environmental stresses.In this study,the emissions of saturated C6-C10 aldehydes from Populus simonii × P.pyramidalis 'Opera 8277' cuttings were examined by using a gas chromatography/mass spectrometry(GC/MS) technique at three levels of light intensity(400,800 and 1 200 μmol·m-2·s-1).A positive correlation between the emissions of these aldehydes and light intensity was found.Moreover,nordi-hydroguaiaretic acid(NDGA),a special inhibitor of lipoxygenase(LOX),significantly inhibited the emissions of C6-C9 aldehydes at three levels of light intensity,but did not influence the emission of decanal(C10).The emissions of C6-C10 aldehydes in NDGA treated poplar cuttings,exhibited the same positive correlation with light intensity.The results indicated that LOX pathway contributes to the emissions of C6-C9 aldehydes,whereas some pathways regulated by light intensity might be a universal mechanism for emissions of C6-C10 aldehydes. 展开更多
关键词 C6-C10 aldehydes light intensity LIPOXYGENASE NDGA Populus simonii × P.pyramidalis 'Opera 8277'
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