It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L...It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L2425 fromthe feces of an ICU patient in China.The isolate belongs to sequence type 11 and carries the antimicrobial resistance genes blaKPC-2,bla_(CTX-M-65) and mcr-8.Through S1-nuclease pulsed-field gel electrophoresis and Southern blotting analysis,we confirmed the presence of a~110 kb plasmid harboring the mcr-8 gene with a conserved structure(inhA-mcr-8-ompR-dgkA-IS903B).Furthermore,another~130 kb plasmid carrying bla_(KPC-2) was detected along with a composite transposon flanked by insertion sequence IS26.Conjugation experiments demonstrated that both pL2425-KPC-2 and pL2425-MCR-8 plasmids were transferable.Importantly,this is the first instance of clinical fecal samples from China containing a K.pneumoniae strain carrying both blaKPC-2 and mcr-8 genes.These findings underscore the significance of surveillance for carbapenemases and mobile colistin–resistance variants.展开更多
This paper proposes a model identification method to get high performance dynamic model of a small unmanned aerial rotorcraft. With the analysis of flight characteristics, a linear dynamic model is constructed by the ...This paper proposes a model identification method to get high performance dynamic model of a small unmanned aerial rotorcraft. With the analysis of flight characteristics, a linear dynamic model is constructed by the small perturbation theory. Using the micro guidance navigation and control module, the system can record the control signals of servos, the state infor- mation of attitude and velocity information in sequence. After the data preprocessing, an adaptive ant colony algorithm is proposed to get optimal parameters of the dynamic model. With the adaptive adjustment of the pheromone in the selection process, the proposed model identification method can escape from local minima traps and get the optimal solution quickly. Performance analysis and experiments are conducted to validate the effectiveness of the identified dynamic model. Compared with real flight data, the identified model generated by the proposed method has a better performance than the model generated by the adaptive genetic algorithm. Based on the identified dynamic model, the small unmanned aerial rotorcraft can generate suitable control parameters to realize stable hovering, turning, and straight flight.展开更多
To the Editor:Acute concomitant esotropia(ACE)is an acquired esotropia with a sudden appearance and no pathologic changes on cerebral imaging.There are various treatments for ACE,including extraocular muscle surgery,b...To the Editor:Acute concomitant esotropia(ACE)is an acquired esotropia with a sudden appearance and no pathologic changes on cerebral imaging.There are various treatments for ACE,including extraocular muscle surgery,botulinum toxin A(BTA)injection,prisms,and divergence training.[1]BTA had been used in the treatment of ACE since it was approved by Food and Drug Administration(FDA,USA)in 1989.[2]Some researchers have confirmed that its effect is stable and has unique advantages in the treatment of ACE.展开更多
Plants produce a rich diversity of biological forms,and the diversity of leaves is especially notable.Mechanisms of leaf morphogenesis have been studied in the past two decades,with a growing focus on the interactive ...Plants produce a rich diversity of biological forms,and the diversity of leaves is especially notable.Mechanisms of leaf morphogenesis have been studied in the past two decades,with a growing focus on the interactive roles of mechanics in recent years.Growth of plant organs involves feedback by mechanical stress:growth induces stress,and stress affects growth and morphogenesis.Although much attention has been given to potential stress-sensing mechanisms and cellular responses,the mechanical principles guiding morphogenesis have not been well understood.Here we synthesize the overarching roles of mechanics and mechanical stress in multilevel and multiple stages of leaf morphogenesis,encompassing leaf primordium initiation,phyllotaxis and venation patterning,and the establishment of complex mature leaf shapes.Moreover,the roles of mechanics at multiscale levels,from subcellular cytoskeletal molecules to single cells to tissues at the organ scale,are articulated.By highlighting the role of mechanical buckling in the formation of three-dimensional leaf shapes,this review integrates the perspectives of mechanics and biology to provide broader insights into the mechanobiology of leaf morphogenesis.展开更多
This paper proposes a fast adaptive fault estimator-based active fault-tolerant control strategy for a quadrotor UAV against multiple actuator faults.A fast adaptive fault estimation algorithm is designed to estimate ...This paper proposes a fast adaptive fault estimator-based active fault-tolerant control strategy for a quadrotor UAV against multiple actuator faults.A fast adaptive fault estimation algorithm is designed to estimate the unknown actuator fault parameters.By synthesizing the fast adaptive fault estimator with the embedded control law,an active fault-tolerant control mechanism is established to compensate the adverse e®ects of multiple actuator faults.The e®ectiveness of the proposed strategy is validated through both numerical simulations and experimental tests.展开更多
Epidermolysis bullosa(EB)is a group of clinically and genetically heterogeneous diseases characterized by trauma-induced mucocutaneous fragility and blister formation.Here,we investigated five Chinese families with EB...Epidermolysis bullosa(EB)is a group of clinically and genetically heterogeneous diseases characterized by trauma-induced mucocutaneous fragility and blister formation.Here,we investigated five Chinese families with EB,and eight variants including a novel nonsense variant(c.47G>A,p.W16*)in LAMA3,a known recurrent variant(c.74C>T,p.P25L)in KRT5,2 novel(c.2531T>A,p.V844E;c.6811_6814del,p.R2271fs)and 4 known(c.6187C>T,p.R2063W;c.7097G>A,p.G2366D;c.8569G>T,p.E2857*;c.3625_3635del,p.S1209fs)variants in COL7A1 were detected.Notably,this study identified a nonsense variant in LAMA3 that causes EB within the Chinese population and revealed that this variant resulted in a reduction in LAMA3 mRNA and protein expression levels by nonsense-mediated mRNA decay.Our study expands the mutation spectra of Chinese patients with EB.展开更多
Dear Editor,Neurofibromatosis type 1(NF1;von Recklinghausen neurofibromatosis;OMIM 162200)is a neurocutaneous genetic disorder that affects approximately one in 2,500 people worldwide(Huson et al.,1989).NF1 is charact...Dear Editor,Neurofibromatosis type 1(NF1;von Recklinghausen neurofibromatosis;OMIM 162200)is a neurocutaneous genetic disorder that affects approximately one in 2,500 people worldwide(Huson et al.,1989).NF1 is characterized by wide clinical variability,including multiple café-au-lait macules(CALMs),fibromatous tumors,axillary or inguinal freckling,Lisch nodules(iris hamartomas),skeletal anomalies,and cognitive impairments(Monroe et al.,2017).展开更多
基金supported by the National Key Research and Development Programof China(2019YFC0840600,2019YFC0840609 and 2020YFE0204300)the National Natural Science Foundation of China(82072314)+3 种基金the Zhejiang Province Natural Science Foundation of China(LHDMZ22H190002 and LY19H160060)the Shandong Provincial Laboratory Project(SYS202202)the Research Project of Jinan Microecological Biomedicine Shandong Laboratory(JNL-2022011B),the Fundamental Research Funds for the Central Universities(2022ZFJH003)the CAMS Innovation Fund for Medical Sciences(2019-I2M-5-045).
文摘It is critical to acknowledge that the coexistence of genes related to carbapenem and polymyxin resistance can exacerbate challenges in antimicrobial therapy.In this study,we identified Klebsiella pneumoniae isolate L2425 fromthe feces of an ICU patient in China.The isolate belongs to sequence type 11 and carries the antimicrobial resistance genes blaKPC-2,bla_(CTX-M-65) and mcr-8.Through S1-nuclease pulsed-field gel electrophoresis and Southern blotting analysis,we confirmed the presence of a~110 kb plasmid harboring the mcr-8 gene with a conserved structure(inhA-mcr-8-ompR-dgkA-IS903B).Furthermore,another~130 kb plasmid carrying bla_(KPC-2) was detected along with a composite transposon flanked by insertion sequence IS26.Conjugation experiments demonstrated that both pL2425-KPC-2 and pL2425-MCR-8 plasmids were transferable.Importantly,this is the first instance of clinical fecal samples from China containing a K.pneumoniae strain carrying both blaKPC-2 and mcr-8 genes.These findings underscore the significance of surveillance for carbapenemases and mobile colistin–resistance variants.
基金The National Natural Science Foundation of China
文摘This paper proposes a model identification method to get high performance dynamic model of a small unmanned aerial rotorcraft. With the analysis of flight characteristics, a linear dynamic model is constructed by the small perturbation theory. Using the micro guidance navigation and control module, the system can record the control signals of servos, the state infor- mation of attitude and velocity information in sequence. After the data preprocessing, an adaptive ant colony algorithm is proposed to get optimal parameters of the dynamic model. With the adaptive adjustment of the pheromone in the selection process, the proposed model identification method can escape from local minima traps and get the optimal solution quickly. Performance analysis and experiments are conducted to validate the effectiveness of the identified dynamic model. Compared with real flight data, the identified model generated by the proposed method has a better performance than the model generated by the adaptive genetic algorithm. Based on the identified dynamic model, the small unmanned aerial rotorcraft can generate suitable control parameters to realize stable hovering, turning, and straight flight.
基金Key Program on Basic Research Project of Universities of Henan(No.22B320016)Scientific Research Fund of National Health Commission-Henan Province Medical Science and Technology Project(No.SB201901013)
文摘To the Editor:Acute concomitant esotropia(ACE)is an acquired esotropia with a sudden appearance and no pathologic changes on cerebral imaging.There are various treatments for ACE,including extraocular muscle surgery,botulinum toxin A(BTA)injection,prisms,and divergence training.[1]BTA had been used in the treatment of ACE since it was approved by Food and Drug Administration(FDA,USA)in 1989.[2]Some researchers have confirmed that its effect is stable and has unique advantages in the treatment of ACE.
基金support from Nanyang Technological University(grant no.M4082428)K.J.H.and C.H.acknowledge support from Nanyang Technological University under its Accelerating Creativity and Excellence(ACE)grant(grant no.NTU-ACE2020-07)+2 种基金supported by the Center for Engineering Mechano Biology,an National Science Foundation(NSF)Science and Technology Center,under grant agreement No.CMMI:15-48571supported by the U.S.Department of Energy(grant no.DE-FG2-84ER13179)support from the Ministry of Education-Singapore,under its Academic Research Fund Tier 1(RT11/20 and RG32/20).
文摘Plants produce a rich diversity of biological forms,and the diversity of leaves is especially notable.Mechanisms of leaf morphogenesis have been studied in the past two decades,with a growing focus on the interactive roles of mechanics in recent years.Growth of plant organs involves feedback by mechanical stress:growth induces stress,and stress affects growth and morphogenesis.Although much attention has been given to potential stress-sensing mechanisms and cellular responses,the mechanical principles guiding morphogenesis have not been well understood.Here we synthesize the overarching roles of mechanics and mechanical stress in multilevel and multiple stages of leaf morphogenesis,encompassing leaf primordium initiation,phyllotaxis and venation patterning,and the establishment of complex mature leaf shapes.Moreover,the roles of mechanics at multiscale levels,from subcellular cytoskeletal molecules to single cells to tissues at the organ scale,are articulated.By highlighting the role of mechanical buckling in the formation of three-dimensional leaf shapes,this review integrates the perspectives of mechanics and biology to provide broader insights into the mechanobiology of leaf morphogenesis.
基金National Key Research and Development Program of China(Grant No.2020YFA0711200)National Natural Science Foundation of China(Grant Nos.61833013 and 61973012)+2 种基金Defense Industrial Technology Development Program(Grant No.JCKY2020601C016)Key Research and Development Program of Zhejiang(Grant No.2021C03158)Science and Technology Key Innovative Project of Hangzhou(Grant No.20182014B06).
文摘This paper proposes a fast adaptive fault estimator-based active fault-tolerant control strategy for a quadrotor UAV against multiple actuator faults.A fast adaptive fault estimation algorithm is designed to estimate the unknown actuator fault parameters.By synthesizing the fast adaptive fault estimator with the embedded control law,an active fault-tolerant control mechanism is established to compensate the adverse e®ects of multiple actuator faults.The e®ectiveness of the proposed strategy is validated through both numerical simulations and experimental tests.
基金This work was financially supported by the National Natural Science Foundation of China(No.81788101)the National Key Research and Development Program of China(No.2016YFC0905100)+1 种基金the CAMS Innovation Fund for Medical Sciences(CIFMS)(No.2016-I2M-1-002)the Natural Science Foundation of Beijing(No.7172167).
文摘Epidermolysis bullosa(EB)is a group of clinically and genetically heterogeneous diseases characterized by trauma-induced mucocutaneous fragility and blister formation.Here,we investigated five Chinese families with EB,and eight variants including a novel nonsense variant(c.47G>A,p.W16*)in LAMA3,a known recurrent variant(c.74C>T,p.P25L)in KRT5,2 novel(c.2531T>A,p.V844E;c.6811_6814del,p.R2271fs)and 4 known(c.6187C>T,p.R2063W;c.7097G>A,p.G2366D;c.8569G>T,p.E2857*;c.3625_3635del,p.S1209fs)variants in COL7A1 were detected.Notably,this study identified a nonsense variant in LAMA3 that causes EB within the Chinese population and revealed that this variant resulted in a reduction in LAMA3 mRNA and protein expression levels by nonsense-mediated mRNA decay.Our study expands the mutation spectra of Chinese patients with EB.
基金supported by the National Key Research and Development Program of China(2016YFC0905100 and 2016YFC1000504)the National Natural Science Foundation of China(81788101)+1 种基金the CAMS Innovation Fund for Medical Sciences(2016-I2M-1002)the Natural Science Foundation of Beijing(7172167)。
文摘Dear Editor,Neurofibromatosis type 1(NF1;von Recklinghausen neurofibromatosis;OMIM 162200)is a neurocutaneous genetic disorder that affects approximately one in 2,500 people worldwide(Huson et al.,1989).NF1 is characterized by wide clinical variability,including multiple café-au-lait macules(CALMs),fibromatous tumors,axillary or inguinal freckling,Lisch nodules(iris hamartomas),skeletal anomalies,and cognitive impairments(Monroe et al.,2017).
