In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named "2019 novel coronavirus(2019-nCoV)" by the World Health Organization(WHO) on 12 January 2020. For it is a n...In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named "2019 novel coronavirus(2019-nCoV)" by the World Health Organization(WHO) on 12 January 2020. For it is a never been experienced respiratory disease before and with infection ability widely and quickly, it attracted the world’s attention but without treatment and control manual. For the request from frontline clinicians and public health professionals of 2019-nCoV infected pneumonia management, an evidence-based guideline urgently needs to be developed. Therefore, we drafted this guideline according to the rapid advice guidelines methodology and general rules of WHO guideline development;we also added the first-hand management data of Zhongnan Hospital of Wuhan University. This guideline includes the guideline methodology, epidemiological characteristics, disease screening and population prevention, diagnosis, treatment and control(including traditional Chinese Medicine), nosocomial infection prevention and control, and disease nursing of the 2019-nCoV. Moreover, we also provide a whole process of a successful treatment case of the severe 2019-nCoV infected pneumonia and experience and lessons of hospital rescue for 2019-nCoV infections. This rapid advice guideline is suitable for the first frontline doctors and nurses, managers of hospitals and healthcare sections, community residents, public health persons, relevant researchers, and all person who are interested in the 2019-nCoV.展开更多
An optimization method is introduced to design the aerodynamic parameters of a dual-spin twodimensional guided projectile with the canards for trajectory correction. The nose guidance component contains two pairs of c...An optimization method is introduced to design the aerodynamic parameters of a dual-spin twodimensional guided projectile with the canards for trajectory correction. The nose guidance component contains two pairs of canards which can provide lift and despin with the projectile for stability. The optimal design algorithm is developed to decide the profiles both of the steering and spinning canards,and their deflection angles are also simulated to meet the needs of trajectory correction capabilities.Finally, the aerodynamic efficiency of the specific canards is discussed according to the CFD simulations.Results that obtained here can be further applied to the exterior ballistics design.展开更多
Objective:The function of euchromatic histone-lysine N-methyltransferase 2(EHMT2)has been studied in several cancers;however,little is known about its role in mantle cell lymphoma(MCL).Thus,this study aimed to charact...Objective:The function of euchromatic histone-lysine N-methyltransferase 2(EHMT2)has been studied in several cancers;however,little is known about its role in mantle cell lymphoma(MCL).Thus,this study aimed to characterize the significance and function of EHMT2 in MCL.Methods:EHMT2 expression in MCL and reactive hyperplasia(RH)were investigated by immunohistochemistry.Genome-wide analysis of DNA methylation was performed on EHMT2+MCL samples.The function of EHMT2 was determined by CCK&flow cytometry,and western blot assays.Gene expression profile analysis was performed before and after EHMT2 knockdown to search for EHMT2-regulated genes.Co-immunoprecipitation(Co-IP)experiments were conducted to identify the proteins interacting with EHMT2.Results:EHMT2 was expressed in 68.57%(24/35)of MCLs but not in any RHs.Genome-wide analysis of DNA methylation on EHMT2+MCLs revealed that multiple members of the HOX,FOX,PAX,SOX,and CDX families were hypermethylated or hypomethylated in EHMT2+MCLs.BIX0I294,a EHMT2 inhibitor,inhibited MCL cell growth and stalled cells in the G1 phase.Additionally,BIX01294 downregulated the expressions of cell cycle proteins,cyclin DI,CDK4,and P21,but upregulated the expressions of apoptosis-related proteins,Bax and caspase-3.Co-IP experiments revealed that EHMT2 interacted with UHRF1,HDAC1,and HDAC2 but not with HDCA3.After EHMT2 knockdown,multiple genes were regulated,including CD5 and CCND1,mostly enriched in the Tec kinase signaling pathway.In addition,several genes(e.g.,MARCH 1,CCDC50,HIP1,and WNT3)were aberrantly methylated in EHMT2+MCLs.Conclusions:For the first time,we determined the significance of EHMT2 in MCL and identified potential EHMT2-regulated genes.展开更多
In this paper we aimed to determine the surface tension of traditional Chinese medicine (TCM) tablet and tried to provide a key reference parameter to control tablet film coating quality. Using contact angle tester, t...In this paper we aimed to determine the surface tension of traditional Chinese medicine (TCM) tablet and tried to provide a key reference parameter to control tablet film coating quality. Using contact angle tester, the Chinese medicine tablet, a famous Fufang Danshen tablet CP as a model example, was determined by Zisman critical surface tension method. Our study showed that the critical surface tension of Fufang Danshen tablet CP was determined to be 18.00 dyne/cm. The Zisman method, hence, can determine successfully the critical surface tension of Chinese medicine tablet and will become an effective manner to control tablet film coating quality.展开更多
Based on the precipitation data and torrential rain disaster data in Zhangjiajie City of Hunan Province from 2016 to 2020,taking the claim cases of the property and cargo insurance(hereinafter referred to as"prop...Based on the precipitation data and torrential rain disaster data in Zhangjiajie City of Hunan Province from 2016 to 2020,taking the claim cases of the property and cargo insurance(hereinafter referred to as"property and cargo insurance")from Hunan Branch of the People’s Insurance Company of China as the research sample,the Dominance Analysis Method was used to determine the influence weights of disaster-causing factors to establish a comprehensive disaster-causing index(I)model of torrential rain.Second,an exponential function was used to fit the relationship between the number of town or street which filed claims of property and cargo insurance and I,then to determine the threshold of I corresponding to different accident levels.The claim cases caused by torrential rain disaster in Zhangjiajie in the flood season of 2021 were selected to verify the I and its threshold.The results showed that the number of property and cargo insurance accidents caused by torrential rain in Zhangjiajie was generally low in east and west but high in middle areas.Among the disaster-causing factors,the weight of the 96-h accumulated precipitation on the scope of accident was the largest,reaching 28.6%.The simulated grades of the scope of accident,the amount of claim and the number of accidents of property and cargo insurance had a high correlation with the grades of actual disasters,and all passed the test at the 0.01 significance level.The threshold test results showed that the consistency rate or accuracy between the predicted level and the actual level of torrential rain disaster-causing cases was 71.4%,in which the predicted values of accuracy for the mild,moderate and severe disaster levels were 70%,70%and 100%,respectively.Therefore,the threshold of I established in this study can be used for the industrial meteorological services related to the property and cargo insurance in Zhangjiajie.展开更多
There are various strategies to conduct tumor microenvironment(TME)stimulus-responsive(e.g.,acid,H_(2)O_(2)or glutathione)nanoreactors for increasing the efficiency of chemodynamic therapy(CDT).Among these,the exploit...There are various strategies to conduct tumor microenvironment(TME)stimulus-responsive(e.g.,acid,H_(2)O_(2)or glutathione)nanoreactors for increasing the efficiency of chemodynamic therapy(CDT).Among these,the exploitation of adenosine triphosphate(ATP,another overexpressed biomarker in TME)-responsive nanoreactors for tumor CDT is still challenging.Herein,the ATP-responsive iron-doped CDs(FeCDs)were firstly prepared and then coassembled with glucose oxidase(GOx)to obtain FeCDs/GOx liposomes as ATP-responsive nanoreactors.Under TME conditions,the nanoreactors initially released FeCDs and GOx.Subsequently,with the existence of ATP,iron ions were rapidly released from the FeCDs to trigger Fenton/Fenton-like reactions for generating·OH.Meanwhile,the T_(1)-weighted magnetic resonance imaging(MRI)was achieved due to the released iron ions.Moreover,the GOx converted endogenous glucose in tumor to gluconic acid and H_(2)O_(2)to satisfy the requirement of·OH generation.In vitro as well as in vivo experiments illustrated that the obtained ATP-responsive CD nanoreactors could be used as a versatile nanotheranostics for simultaneously T_(1)-weighted MRI-guided tumor CDT.This work presents a new ATP-responsive nanoreactor with selfsupplied H_(2)O_(2)for multifunctional nanotheranostic applications.展开更多
Photodynamic therapy(PDT)has been emerged as a promising modality for cancer treatment.However,the development of drug delivery system enabling continuous release of photosensitizers(PSs)for long-term PDT treatment st...Photodynamic therapy(PDT)has been emerged as a promising modality for cancer treatment.However,the development of drug delivery system enabling continuous release of photosensitizers(PSs)for long-term PDT treatment still remains challenges.Herein,a H_(2)O_(2)-responsive injectable hydrogel,covalently crosslinked by N^(1)-(4-boronobenzyl)-N^(3)-(4-boronophenyl)-N^(1),N^(1),N^(3),N^(3)-tetramethylpropane-1,3-diaminium(TSPBA)with PVA containing polythiophene quaternary ammonium salt(PT2)polymer dots(PDots)as a photosensitizer was fabricated.Under the stimulation of H_(2)O_(2),the obtained injectable hydrogel gradually degrades and releases PDots.In vitro experiments suggested that the released PDots could realize efficient tumor cells inhibition through its robust singlet oxygen generation capability upon 577 nm laser irradiation.In vivo studies demonstrated a sustained retention of PDots for at least 7 days following single-dose administration,facilitating efficient tumor inhibition with light treatments for 3 times without apparent biotoxicity.This work presents an innovative polymer dots-based composite local drug delivery system for long-term PDT in cancer treatment.展开更多
基金supported(in part)by the Entrusted Project of National Center for Medical Service Administration,National Health and Family Planning Commission China(No.[2019]099)the First Level Funding of the Second Medical Leading Talent Project in Hubei Provincethe Special Project for Emergency of the Ministry of Science and Technology(2020YFC0841300)。
文摘In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named "2019 novel coronavirus(2019-nCoV)" by the World Health Organization(WHO) on 12 January 2020. For it is a never been experienced respiratory disease before and with infection ability widely and quickly, it attracted the world’s attention but without treatment and control manual. For the request from frontline clinicians and public health professionals of 2019-nCoV infected pneumonia management, an evidence-based guideline urgently needs to be developed. Therefore, we drafted this guideline according to the rapid advice guidelines methodology and general rules of WHO guideline development;we also added the first-hand management data of Zhongnan Hospital of Wuhan University. This guideline includes the guideline methodology, epidemiological characteristics, disease screening and population prevention, diagnosis, treatment and control(including traditional Chinese Medicine), nosocomial infection prevention and control, and disease nursing of the 2019-nCoV. Moreover, we also provide a whole process of a successful treatment case of the severe 2019-nCoV infected pneumonia and experience and lessons of hospital rescue for 2019-nCoV infections. This rapid advice guideline is suitable for the first frontline doctors and nurses, managers of hospitals and healthcare sections, community residents, public health persons, relevant researchers, and all person who are interested in the 2019-nCoV.
文摘An optimization method is introduced to design the aerodynamic parameters of a dual-spin twodimensional guided projectile with the canards for trajectory correction. The nose guidance component contains two pairs of canards which can provide lift and despin with the projectile for stability. The optimal design algorithm is developed to decide the profiles both of the steering and spinning canards,and their deflection angles are also simulated to meet the needs of trajectory correction capabilities.Finally, the aerodynamic efficiency of the specific canards is discussed according to the CFD simulations.Results that obtained here can be further applied to the exterior ballistics design.
基金This study was supported by the National Natural Science Foundation of China(Grant No.81272277)the Natural Science Foundation of Shandong Province(Grant No.ZR2018MH020).
文摘Objective:The function of euchromatic histone-lysine N-methyltransferase 2(EHMT2)has been studied in several cancers;however,little is known about its role in mantle cell lymphoma(MCL).Thus,this study aimed to characterize the significance and function of EHMT2 in MCL.Methods:EHMT2 expression in MCL and reactive hyperplasia(RH)were investigated by immunohistochemistry.Genome-wide analysis of DNA methylation was performed on EHMT2+MCL samples.The function of EHMT2 was determined by CCK&flow cytometry,and western blot assays.Gene expression profile analysis was performed before and after EHMT2 knockdown to search for EHMT2-regulated genes.Co-immunoprecipitation(Co-IP)experiments were conducted to identify the proteins interacting with EHMT2.Results:EHMT2 was expressed in 68.57%(24/35)of MCLs but not in any RHs.Genome-wide analysis of DNA methylation on EHMT2+MCLs revealed that multiple members of the HOX,FOX,PAX,SOX,and CDX families were hypermethylated or hypomethylated in EHMT2+MCLs.BIX0I294,a EHMT2 inhibitor,inhibited MCL cell growth and stalled cells in the G1 phase.Additionally,BIX01294 downregulated the expressions of cell cycle proteins,cyclin DI,CDK4,and P21,but upregulated the expressions of apoptosis-related proteins,Bax and caspase-3.Co-IP experiments revealed that EHMT2 interacted with UHRF1,HDAC1,and HDAC2 but not with HDCA3.After EHMT2 knockdown,multiple genes were regulated,including CD5 and CCND1,mostly enriched in the Tec kinase signaling pathway.In addition,several genes(e.g.,MARCH 1,CCDC50,HIP1,and WNT3)were aberrantly methylated in EHMT2+MCLs.Conclusions:For the first time,we determined the significance of EHMT2 in MCL and identified potential EHMT2-regulated genes.
文摘In this paper we aimed to determine the surface tension of traditional Chinese medicine (TCM) tablet and tried to provide a key reference parameter to control tablet film coating quality. Using contact angle tester, the Chinese medicine tablet, a famous Fufang Danshen tablet CP as a model example, was determined by Zisman critical surface tension method. Our study showed that the critical surface tension of Fufang Danshen tablet CP was determined to be 18.00 dyne/cm. The Zisman method, hence, can determine successfully the critical surface tension of Chinese medicine tablet and will become an effective manner to control tablet film coating quality.
基金Supported by Key Project of Hunan Meteorological Bureau in 2021(XQKJ21A006)。
文摘Based on the precipitation data and torrential rain disaster data in Zhangjiajie City of Hunan Province from 2016 to 2020,taking the claim cases of the property and cargo insurance(hereinafter referred to as"property and cargo insurance")from Hunan Branch of the People’s Insurance Company of China as the research sample,the Dominance Analysis Method was used to determine the influence weights of disaster-causing factors to establish a comprehensive disaster-causing index(I)model of torrential rain.Second,an exponential function was used to fit the relationship between the number of town or street which filed claims of property and cargo insurance and I,then to determine the threshold of I corresponding to different accident levels.The claim cases caused by torrential rain disaster in Zhangjiajie in the flood season of 2021 were selected to verify the I and its threshold.The results showed that the number of property and cargo insurance accidents caused by torrential rain in Zhangjiajie was generally low in east and west but high in middle areas.Among the disaster-causing factors,the weight of the 96-h accumulated precipitation on the scope of accident was the largest,reaching 28.6%.The simulated grades of the scope of accident,the amount of claim and the number of accidents of property and cargo insurance had a high correlation with the grades of actual disasters,and all passed the test at the 0.01 significance level.The threshold test results showed that the consistency rate or accuracy between the predicted level and the actual level of torrential rain disaster-causing cases was 71.4%,in which the predicted values of accuracy for the mild,moderate and severe disaster levels were 70%,70%and 100%,respectively.Therefore,the threshold of I established in this study can be used for the industrial meteorological services related to the property and cargo insurance in Zhangjiajie.
基金supported by the National Key Research and Development Program of China(2022YFA1207600)National Natural Science Foundation of China(51972315,21873110,52272052,61720106014)project ZR2023QE322 supported by Shandong Provincial Natural Science Foundation。
文摘There are various strategies to conduct tumor microenvironment(TME)stimulus-responsive(e.g.,acid,H_(2)O_(2)or glutathione)nanoreactors for increasing the efficiency of chemodynamic therapy(CDT).Among these,the exploitation of adenosine triphosphate(ATP,another overexpressed biomarker in TME)-responsive nanoreactors for tumor CDT is still challenging.Herein,the ATP-responsive iron-doped CDs(FeCDs)were firstly prepared and then coassembled with glucose oxidase(GOx)to obtain FeCDs/GOx liposomes as ATP-responsive nanoreactors.Under TME conditions,the nanoreactors initially released FeCDs and GOx.Subsequently,with the existence of ATP,iron ions were rapidly released from the FeCDs to trigger Fenton/Fenton-like reactions for generating·OH.Meanwhile,the T_(1)-weighted magnetic resonance imaging(MRI)was achieved due to the released iron ions.Moreover,the GOx converted endogenous glucose in tumor to gluconic acid and H_(2)O_(2)to satisfy the requirement of·OH generation.In vitro as well as in vivo experiments illustrated that the obtained ATP-responsive CD nanoreactors could be used as a versatile nanotheranostics for simultaneously T_(1)-weighted MRI-guided tumor CDT.This work presents a new ATP-responsive nanoreactor with selfsupplied H_(2)O_(2)for multifunctional nanotheranostic applications.
基金financially supported by the National Natural Science Foundation of China(Nos.52272052 and 51972315)。
文摘Photodynamic therapy(PDT)has been emerged as a promising modality for cancer treatment.However,the development of drug delivery system enabling continuous release of photosensitizers(PSs)for long-term PDT treatment still remains challenges.Herein,a H_(2)O_(2)-responsive injectable hydrogel,covalently crosslinked by N^(1)-(4-boronobenzyl)-N^(3)-(4-boronophenyl)-N^(1),N^(1),N^(3),N^(3)-tetramethylpropane-1,3-diaminium(TSPBA)with PVA containing polythiophene quaternary ammonium salt(PT2)polymer dots(PDots)as a photosensitizer was fabricated.Under the stimulation of H_(2)O_(2),the obtained injectable hydrogel gradually degrades and releases PDots.In vitro experiments suggested that the released PDots could realize efficient tumor cells inhibition through its robust singlet oxygen generation capability upon 577 nm laser irradiation.In vivo studies demonstrated a sustained retention of PDots for at least 7 days following single-dose administration,facilitating efficient tumor inhibition with light treatments for 3 times without apparent biotoxicity.This work presents an innovative polymer dots-based composite local drug delivery system for long-term PDT in cancer treatment.
