BACKGROUND Fracture nonunion represents a challenging complication during fracture repair,often necessitating surgical intervention.Teriparatide,a recombinant human parathyroid hormone,has demonstrated promise in enha...BACKGROUND Fracture nonunion represents a challenging complication during fracture repair,often necessitating surgical intervention.Teriparatide,a recombinant human parathyroid hormone,has demonstrated promise in enhancing fracture healing,although its efficacy in treating established nonunion remains under investigation.CASE SUMMARY We report a case of a 27-year-old male who presented with a right humerus fracture following a traffic accident.Despite undergoing open reduction and internal fixation,the fracture resulted in a delayed union and subsequent nonunion.After 4 years of conservative management,teriparatide treatment was initiated due to persistent nonunion.Teriparatide injections were administered daily for 6 months,resulting in complete fracture healing and resolution of pain.CONCLUSION Our case demonstrates the successful use of teriparatide in treating a prolonged nonunion of a humerus fracture.Teriparatide may provide a valuable therapeutic option for established bone nonunion,even in cases that have not responded to conservative treatments.展开更多
Metallic Li is a promising anode material for high energy density batteries but it suffers from poor stability and formation of unsafe dendrites. Previous studies demonstrated that 3 D metal foams are able to improve ...Metallic Li is a promising anode material for high energy density batteries but it suffers from poor stability and formation of unsafe dendrites. Previous studies demonstrated that 3 D metal foams are able to improve the stability of Li metal but the properties of these foams are inherently limited. Here we report a facile surface modification approach via magnetron sputtering of mixed oxides that effectively modulate the properties of Cu foams for supporting Li metal with remarkable stability. We discovered that hybrid Li anodes with Li metal thermally infused to aluminum-zinc oxides(AZO) coated Cu foams have significantly improved stability and reactivity compared with pristine Li foils and Li infused to unmodified Cu foams. Full cells assembled with a Li Fe PO4 cathode and a hybrid anode maintained low and stable charge-transfer resistance(<50) during 500 cycles in carbonate electrolytes, and exhibited superior rate capability(~100 m Ah g-1 at 20 C) along with better electrochemical reversibility and surface stability. The AZO modified Cu foams had superior mechanical strength and afforded the hybrid anodes with minimized volume change without the formation of dendrites during battery cycling. The rational construction of surface architecture to precisely control Li plating and stripping may have great implications for the practical applications of Li metal batteries.展开更多
This work outlines an experimental investigation of grain-size-dependent structure evolution under tension in nickel with a grain size gradient.Two opposite and competing processes,grain refinement and coarsening,were...This work outlines an experimental investigation of grain-size-dependent structure evolution under tension in nickel with a grain size gradient.Two opposite and competing processes,grain refinement and coarsening,were examined within one specimen,due to the widely ranging grain size in gradient-structured(GS)Ni.A tensioninduced minimum grain size of approximately 280 nm was determined in GS Ni,which is comparable to those obtained by severe plastic deformation processes.The minimum grain size was phenomenologically explained using a dislocation model.Below the minimum grain size,the Ni’s grain coarsening ability peaked at approximately 50 nm and progressively decreased with decreasing grain size,showing an inverse grain-size-dependent coarsening tendency.Moreover,this inverse grain coarsening behavior was related to a transition in the deformation mechanism,through which the deformation process was accommodated more by partial dislocation than by full dislocation below a critical grain size.This was confirmed by observation of the microstructure and low temperature tensile testing results.This work demonstrates a high-throughput strategy for exploring the minimum grain size and grain-size-dependent coarsening in metals.展开更多
Imatinib mesylate(IM)is the first-line treatment for Philadelphia(Ph)chromosomal positive leukemia by inhibiting phosphorylation of substrates via binding to the ABL kinase domain.Because of the drug resistance,side e...Imatinib mesylate(IM)is the first-line treatment for Philadelphia(Ph)chromosomal positive leukemia by inhibiting phosphorylation of substrates via binding to the ABL kinase domain.Because of the drug resistance,side effects and the high cost of IM,it is necessary to find anti-cancer drugs with relatively low toxicity and cost,and enhanced efficacy,such as traditional Chinese medicines(TCMs).As one of TCMs,Huai Qi Huang(HQH)was chosen to treat BV173 and K562 cells.Various concentrations of HQH were added to cells for 24-72 h.Co-treatment of HQH and trametinib,an MEK inhibitor,was used to verify the synergistic effects on cell viability and apoptosis.Knockdown and overexpression of mitogen-activated protein kinase kinase 4(MEK4)were implemented to demonstrate the role of MEK in cell apoptosis.Cell viability and apoptosis were measured by cell counting kit-8 assay(CCK8)and flow cytometry,respectively.Western blotting and real-time quantitative PCR(RT-qPCR)were used to assess protein and mRNA expression levels,respectively.The results showed that HQH inhibited survival and promoted apoptosis of BV173 and K562 cells in a dose-dependent manner,accompanied with down-regulation of PRKCH mRNA as well as CRAF,MEK4,phospho-ERK(pERK)and BCL2 proteins,and up-regulation of cleaved caspase3 protein.Co-treatment of HQH and trametinib had.a synergistic effect on inhibiting survival and promoting apoptosis.MEK4 knockdown increased apoptosis,and had a synergistic effeet with HQH.In contrast,MEK4 overexpression decreased.apoptosis,and had the opposite efect with HQH.Collectively,the results of this study may identify a therapeutic mechanism of HQH on promoting apoptosis,and provide a potential option for treatment of Pht leukemia.展开更多
目的探讨微滴数字聚合酶链反应(ddPCR)在重症急性胰腺炎(SAP)合并疑似血流感染(BSI)病原学诊断中的价值。方法选取2022年7—9月某院重症医学科收治的SAP患者,在疑似BSI发作时同步采集静脉血进行ddPCR检测和血培养(BC)及药敏试验(AST),...目的探讨微滴数字聚合酶链反应(ddPCR)在重症急性胰腺炎(SAP)合并疑似血流感染(BSI)病原学诊断中的价值。方法选取2022年7—9月某院重症医学科收治的SAP患者,在疑似BSI发作时同步采集静脉血进行ddPCR检测和血培养(BC)及药敏试验(AST),记录两种检测方法的耗时,比较ddPCR与BC的检测结果,计算ddPCR的病原学诊断效能,并探讨ddPCR检测病原菌载量值与感染指标水平的相关性。结果共纳入22例患者,采集52份静脉血标本进行检测,BC阳性17份(32.7%),检出病原体29株;ddPCR阳性41份(78.8%),检测出病原体73株。ddPCR耗时低于BC[(0.16±0.03)d VS(5.92±1.20)d,P<0.001]。在ddPCR检测范围内,以BC为金标准,ddPCR检测的灵敏度和特异度分别为80.0%、28.6%;联合检测前1周内非血标本微生物证据综合判定BSI,ddPCR检测的灵敏度和特异度分别提高至91.9%、76.9%。ddPCR耐药基因检测中,19份检出bla KPC,9份检出bla NDM/IMP,6份检出V an A/V an M,5份检出mec A。相关性分析显示病原菌载量值与C反应蛋白、降钙素原水平呈正相关(r分别为0.347、0.414,均P<0.05)。结论ddPCR作为一种辅助BC诊断BSI的检测方法具有灵敏度高、耗时低等优势,值得进一步探讨其在临床中的应用。展开更多
Predictive Business Process Monitoring(PBPM)is a significant research area in Business Process Management(BPM)aimed at accurately forecasting future behavioral events.At present,deep learning methods are widely cited ...Predictive Business Process Monitoring(PBPM)is a significant research area in Business Process Management(BPM)aimed at accurately forecasting future behavioral events.At present,deep learning methods are widely cited in PBPM research,but no method has been effective in fusing data information into the control flow for multi-perspective process prediction.Therefore,this paper proposes a process prediction method based on the hierarchical BERT and multi-perspective data fusion.Firstly,the first layer BERT network learns the correlations between different category attribute data.Then,the attribute data is integrated into a weighted event-level feature vector and input into the second layer BERT network to learn the impact and priority relationship of each event on future predicted events.Next,the multi-head attention mechanism within the framework is visualized for analysis,helping to understand the decision-making logic of the framework and providing visual predictions.Finally,experimental results show that the predictive accuracy of the framework surpasses the current state-of-the-art research methods and significantly enhances the predictive performance of BPM.展开更多
In recent years, antibiotic pollution has become a serious threat to human health. In this study, a gas-liquid discharge plasma is developed to degrade ciprofloxacin hydrochloride in a multiphase mixed system containi...In recent years, antibiotic pollution has become a serious threat to human health. In this study, a gas-liquid discharge plasma is developed to degrade ciprofloxacin hydrochloride in a multiphase mixed system containing inorganic and organic impurities. The discharge characteristics are analyzed by diagnosing the applied voltage and discharge current waveforms, as well as the optical emission spectra. The work investigates how degradation efficiency is affected by applied voltage, gas flow rate, treatment time, initial concentration as well as the addition of γ-Al_(2)O_(3) pellets and peanut straw. After 70 min, the degradation efficiency of ciprofloxacin hydrochloride in the multiphase mixed system reached 99.6%. Its removal efficiency increases as the initial concentration decreases and the applied voltage increases. Besides, there is still a good degradation efficiency of ciprofloxacin hydrochloride with the addition of peanut straw.The degradation mechanism of ciprofloxacin hydrochloride is investigated through the analysis of degraded intermediates and reactive species.展开更多
The long sought cubic gauche polymeric nitrogen(cg-N)consisting of N-N single bonds has been synthesized by one pot route using sodium azide as a precursor at ambient conditions.The recrystallization process was desig...The long sought cubic gauche polymeric nitrogen(cg-N)consisting of N-N single bonds has been synthesized by one pot route using sodium azide as a precursor at ambient conditions.The recrystallization process was designed to expose crystal faces with low activation energy that facilitates initiating the polymeric reaction at ambient conditions.The azide was considered as a precursor due to the low energy barrier in transforming double bonded N=N to single bonded cg-N.Raman spectrum measurements detected the emerging vibron peaks at 635 cm^(−1) for the polymerized sodium azide samples,demonstrating the formation of cg-N with N-N single bonds.Different from traditional high-pressure technique and recently developed plasma enhanced chemical vapor deposition method,the route achieves the quantitative synthesis of cg-N at ambient conditions.The one pot synthesis of cg-N offers potential for further scaling up production as well as practical applications of polymeric nitrogen based materials as high energy density materials.展开更多
基金Supported by National Natural Science Foundation of China,No.82172441Suzhou City Major Disease Multicenter Clinical Research Project,No.DZXYJ202312+2 种基金Special Funding for Jiangsu Province Science and Technology Plan(Key Research and Development Program for Social Development),No.BE2023737Gusu Health Talent Plan Scientific Research Project,No.GSWS2022109Postgraduate Research and Practice Innovation Program of Jiangsu Province,No.SJCX24_2446.
文摘BACKGROUND Fracture nonunion represents a challenging complication during fracture repair,often necessitating surgical intervention.Teriparatide,a recombinant human parathyroid hormone,has demonstrated promise in enhancing fracture healing,although its efficacy in treating established nonunion remains under investigation.CASE SUMMARY We report a case of a 27-year-old male who presented with a right humerus fracture following a traffic accident.Despite undergoing open reduction and internal fixation,the fracture resulted in a delayed union and subsequent nonunion.After 4 years of conservative management,teriparatide treatment was initiated due to persistent nonunion.Teriparatide injections were administered daily for 6 months,resulting in complete fracture healing and resolution of pain.CONCLUSION Our case demonstrates the successful use of teriparatide in treating a prolonged nonunion of a humerus fracture.Teriparatide may provide a valuable therapeutic option for established bone nonunion,even in cases that have not responded to conservative treatments.
基金The financial supports of the National Natural Science Foundation of China(Grant Nos.51572060,51702067 and 51671074)Special Financial Grant from the China Postdoctoral Science Foundation(No.2017T100239)+1 种基金General Financial Grant from the China Postdoctoral Science Foundation(No.2016M590279)the startup grants from Northern Illinois University。
文摘Metallic Li is a promising anode material for high energy density batteries but it suffers from poor stability and formation of unsafe dendrites. Previous studies demonstrated that 3 D metal foams are able to improve the stability of Li metal but the properties of these foams are inherently limited. Here we report a facile surface modification approach via magnetron sputtering of mixed oxides that effectively modulate the properties of Cu foams for supporting Li metal with remarkable stability. We discovered that hybrid Li anodes with Li metal thermally infused to aluminum-zinc oxides(AZO) coated Cu foams have significantly improved stability and reactivity compared with pristine Li foils and Li infused to unmodified Cu foams. Full cells assembled with a Li Fe PO4 cathode and a hybrid anode maintained low and stable charge-transfer resistance(<50) during 500 cycles in carbonate electrolytes, and exhibited superior rate capability(~100 m Ah g-1 at 20 C) along with better electrochemical reversibility and surface stability. The AZO modified Cu foams had superior mechanical strength and afforded the hybrid anodes with minimized volume change without the formation of dendrites during battery cycling. The rational construction of surface architecture to precisely control Li plating and stripping may have great implications for the practical applications of Li metal batteries.
基金financial support received from the National Key Research and Development Program of China(Grant no.2017YFB0702003)the National Natural Science Foundation of China(Grant no.51471165).
文摘This work outlines an experimental investigation of grain-size-dependent structure evolution under tension in nickel with a grain size gradient.Two opposite and competing processes,grain refinement and coarsening,were examined within one specimen,due to the widely ranging grain size in gradient-structured(GS)Ni.A tensioninduced minimum grain size of approximately 280 nm was determined in GS Ni,which is comparable to those obtained by severe plastic deformation processes.The minimum grain size was phenomenologically explained using a dislocation model.Below the minimum grain size,the Ni’s grain coarsening ability peaked at approximately 50 nm and progressively decreased with decreasing grain size,showing an inverse grain-size-dependent coarsening tendency.Moreover,this inverse grain coarsening behavior was related to a transition in the deformation mechanism,through which the deformation process was accommodated more by partial dislocation than by full dislocation below a critical grain size.This was confirmed by observation of the microstructure and low temperature tensile testing results.This work demonstrates a high-throughput strategy for exploring the minimum grain size and grain-size-dependent coarsening in metals.
基金The present study was supported by the National Natural Science Foundation of China(No.81700147).
文摘Imatinib mesylate(IM)is the first-line treatment for Philadelphia(Ph)chromosomal positive leukemia by inhibiting phosphorylation of substrates via binding to the ABL kinase domain.Because of the drug resistance,side effects and the high cost of IM,it is necessary to find anti-cancer drugs with relatively low toxicity and cost,and enhanced efficacy,such as traditional Chinese medicines(TCMs).As one of TCMs,Huai Qi Huang(HQH)was chosen to treat BV173 and K562 cells.Various concentrations of HQH were added to cells for 24-72 h.Co-treatment of HQH and trametinib,an MEK inhibitor,was used to verify the synergistic effects on cell viability and apoptosis.Knockdown and overexpression of mitogen-activated protein kinase kinase 4(MEK4)were implemented to demonstrate the role of MEK in cell apoptosis.Cell viability and apoptosis were measured by cell counting kit-8 assay(CCK8)and flow cytometry,respectively.Western blotting and real-time quantitative PCR(RT-qPCR)were used to assess protein and mRNA expression levels,respectively.The results showed that HQH inhibited survival and promoted apoptosis of BV173 and K562 cells in a dose-dependent manner,accompanied with down-regulation of PRKCH mRNA as well as CRAF,MEK4,phospho-ERK(pERK)and BCL2 proteins,and up-regulation of cleaved caspase3 protein.Co-treatment of HQH and trametinib had.a synergistic effect on inhibiting survival and promoting apoptosis.MEK4 knockdown increased apoptosis,and had a synergistic effeet with HQH.In contrast,MEK4 overexpression decreased.apoptosis,and had the opposite efect with HQH.Collectively,the results of this study may identify a therapeutic mechanism of HQH on promoting apoptosis,and provide a potential option for treatment of Pht leukemia.
文摘目的探讨微滴数字聚合酶链反应(ddPCR)在重症急性胰腺炎(SAP)合并疑似血流感染(BSI)病原学诊断中的价值。方法选取2022年7—9月某院重症医学科收治的SAP患者,在疑似BSI发作时同步采集静脉血进行ddPCR检测和血培养(BC)及药敏试验(AST),记录两种检测方法的耗时,比较ddPCR与BC的检测结果,计算ddPCR的病原学诊断效能,并探讨ddPCR检测病原菌载量值与感染指标水平的相关性。结果共纳入22例患者,采集52份静脉血标本进行检测,BC阳性17份(32.7%),检出病原体29株;ddPCR阳性41份(78.8%),检测出病原体73株。ddPCR耗时低于BC[(0.16±0.03)d VS(5.92±1.20)d,P<0.001]。在ddPCR检测范围内,以BC为金标准,ddPCR检测的灵敏度和特异度分别为80.0%、28.6%;联合检测前1周内非血标本微生物证据综合判定BSI,ddPCR检测的灵敏度和特异度分别提高至91.9%、76.9%。ddPCR耐药基因检测中,19份检出bla KPC,9份检出bla NDM/IMP,6份检出V an A/V an M,5份检出mec A。相关性分析显示病原菌载量值与C反应蛋白、降钙素原水平呈正相关(r分别为0.347、0.414,均P<0.05)。结论ddPCR作为一种辅助BC诊断BSI的检测方法具有灵敏度高、耗时低等优势,值得进一步探讨其在临床中的应用。
基金Supported by the National Natural Science Foundation,China(No.61402011)the Open Project Program of the Key Laboratory of Embedded System and Service Computing of Ministry of Education(No.ESSCKF2021-05).
文摘Predictive Business Process Monitoring(PBPM)is a significant research area in Business Process Management(BPM)aimed at accurately forecasting future behavioral events.At present,deep learning methods are widely cited in PBPM research,but no method has been effective in fusing data information into the control flow for multi-perspective process prediction.Therefore,this paper proposes a process prediction method based on the hierarchical BERT and multi-perspective data fusion.Firstly,the first layer BERT network learns the correlations between different category attribute data.Then,the attribute data is integrated into a weighted event-level feature vector and input into the second layer BERT network to learn the impact and priority relationship of each event on future predicted events.Next,the multi-head attention mechanism within the framework is visualized for analysis,helping to understand the decision-making logic of the framework and providing visual predictions.Finally,experimental results show that the predictive accuracy of the framework surpasses the current state-of-the-art research methods and significantly enhances the predictive performance of BPM.
基金supported by National Natural Science Foundations of China (Nos. 52307163 and 12305279)the China Postdoctoral Science Foundation (Nos. 2023M740498 and 2022M710590)Postdoctoral Fellowship Program of CPSF (No. GZC20230348)。
文摘In recent years, antibiotic pollution has become a serious threat to human health. In this study, a gas-liquid discharge plasma is developed to degrade ciprofloxacin hydrochloride in a multiphase mixed system containing inorganic and organic impurities. The discharge characteristics are analyzed by diagnosing the applied voltage and discharge current waveforms, as well as the optical emission spectra. The work investigates how degradation efficiency is affected by applied voltage, gas flow rate, treatment time, initial concentration as well as the addition of γ-Al_(2)O_(3) pellets and peanut straw. After 70 min, the degradation efficiency of ciprofloxacin hydrochloride in the multiphase mixed system reached 99.6%. Its removal efficiency increases as the initial concentration decreases and the applied voltage increases. Besides, there is still a good degradation efficiency of ciprofloxacin hydrochloride with the addition of peanut straw.The degradation mechanism of ciprofloxacin hydrochloride is investigated through the analysis of degraded intermediates and reactive species.
文摘The long sought cubic gauche polymeric nitrogen(cg-N)consisting of N-N single bonds has been synthesized by one pot route using sodium azide as a precursor at ambient conditions.The recrystallization process was designed to expose crystal faces with low activation energy that facilitates initiating the polymeric reaction at ambient conditions.The azide was considered as a precursor due to the low energy barrier in transforming double bonded N=N to single bonded cg-N.Raman spectrum measurements detected the emerging vibron peaks at 635 cm^(−1) for the polymerized sodium azide samples,demonstrating the formation of cg-N with N-N single bonds.Different from traditional high-pressure technique and recently developed plasma enhanced chemical vapor deposition method,the route achieves the quantitative synthesis of cg-N at ambient conditions.The one pot synthesis of cg-N offers potential for further scaling up production as well as practical applications of polymeric nitrogen based materials as high energy density materials.
