Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme.To some extent,the performance of immobilized enzyme depends on the choi...Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme.To some extent,the performance of immobilized enzyme depends on the choice of carrier material.Therefore,the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers.In this work,a novel organic–inorganic hybrid material,nickel-carnosine complex(NiCar),was synthesized for the first time by solvothermal method.The obtained NiCar exhibits spherical morphology,hierarchical porosity and abundant unsaturated coordination nickel ions,which provide excellent anchoring sites for the immobilization of proteins.His-tagged organophosphate-degrading enzyme(Opd A)and x-transaminase(ω-TA)were used as model enzymes to evaluate the performance of NiCar as a carrier.By a simple adsorption process,the enzyme molecules can be fixed on the particles of NiCar,and the stability and reusability are significantly improved.The analysis of protein adsorption on NiCar verified that the affinity adsorption between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process,which provided an idea way for the development of new enzyme immobilization carriers.展开更多
In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts;however,it is limited by the conditions of metal–organic framework synthesis and its acid-base stability.Herein,a b...In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts;however,it is limited by the conditions of metal–organic framework synthesis and its acid-base stability.Herein,a biocatalytic platform with improved acid-base stability was constructed via a one-pot method using bismuth-ellagic acid as the carrier.Bismuth-ellagic acid is a green phenol-based metal–organic framework whose organic precursor is extracted from natural plants.After encapsulation,the stability,especially the acid-base stability,of amyloglucosidases@bismuth-ellagic acid was enhanced,which remained stable over a wide pH range(2–12)and achieved multiple recycling.By selecting a suitable buffer,bismuth-ellagic acid can encapsulate different types of enzymes and enable interactions between the encapsulated enzymes and cofactors,as well as between multiple enzymes.The green precursor,simple and convenient preparation process provided a versatile strategy for enzymes encapsulation.展开更多
文摘Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme.To some extent,the performance of immobilized enzyme depends on the choice of carrier material.Therefore,the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers.In this work,a novel organic–inorganic hybrid material,nickel-carnosine complex(NiCar),was synthesized for the first time by solvothermal method.The obtained NiCar exhibits spherical morphology,hierarchical porosity and abundant unsaturated coordination nickel ions,which provide excellent anchoring sites for the immobilization of proteins.His-tagged organophosphate-degrading enzyme(Opd A)and x-transaminase(ω-TA)were used as model enzymes to evaluate the performance of NiCar as a carrier.By a simple adsorption process,the enzyme molecules can be fixed on the particles of NiCar,and the stability and reusability are significantly improved.The analysis of protein adsorption on NiCar verified that the affinity adsorption between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process,which provided an idea way for the development of new enzyme immobilization carriers.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.22178083,22078081 and 21878068)the Natural Science Foundation of Tianjin China(Grant No.20JCYBJC00530)+3 种基金the Hebei Key Research and Development Project(Grant No.20372802D)Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering(Grant No.SKLBEE2020011)Science Technology Research Project of Higher Education of Hebei Province(Grant No.QN2021045)Tianjin Enterprise Science and Technology Commissioner Project(Grant No.21YDTPJC00810).
文摘In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts;however,it is limited by the conditions of metal–organic framework synthesis and its acid-base stability.Herein,a biocatalytic platform with improved acid-base stability was constructed via a one-pot method using bismuth-ellagic acid as the carrier.Bismuth-ellagic acid is a green phenol-based metal–organic framework whose organic precursor is extracted from natural plants.After encapsulation,the stability,especially the acid-base stability,of amyloglucosidases@bismuth-ellagic acid was enhanced,which remained stable over a wide pH range(2–12)and achieved multiple recycling.By selecting a suitable buffer,bismuth-ellagic acid can encapsulate different types of enzymes and enable interactions between the encapsulated enzymes and cofactors,as well as between multiple enzymes.The green precursor,simple and convenient preparation process provided a versatile strategy for enzymes encapsulation.
