In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for n...In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for neurons resistant to injury. After colchicine injection, the number of nestin^＋ cholinergic neurons was decreased at 1 day, but increased at 3 days and peaked at 14-28 days. The quantity of nestincholinergic neurons, parvalbumin-positive neurons and choline acetyl transferase-positive neurons decreased gradually. Our results indicate that nestin^＋ cholinergic neurons possess better tolerance to colchicine-induced neurotoxicity.展开更多
In early life, the immune system plays an essential role in brain development. In our study, the immunopotentiator thymosin alpha-1（Ta1） was peripherally administered to neonatal mice to explore whether the peripher...In early life, the immune system plays an essential role in brain development. In our study, the immunopotentiator thymosin alpha-1（Ta1） was peripherally administered to neonatal mice to explore whether the peripheral immunopotentiator affects neurodevelopment and cognition, and to further investigate the relevant mechanism. Compared with the control group, the Ta1 mice displayed better cognitive abilities in early life. The numbers of 5-bromodeoxyuridine（Brd U）＋, nestin＋,T-box transcription factor 2（Tbr2）＋, Brd U＋/doublecortin（DCX）＋, Brd U＋/ionized calcium-binding adaptor molecule 1（Iba1）＋, and Brd U＋/neuronal nuclei（Neu N）＋ cells in the hippocampus were increased in the Ta1 group,accompanied by increased interleukin-4（IL-4）, interferon-gamma, brain-derived neurotrophic factor, nerve growth factor, and insulin-like growth factor-1 as well as decreased IL-6 and tumor necrosis factor-a. Furthermore, the Ta1-group showed a Th1-polarized immune response, and the neurotrophic factors were positively associated with the Th1/Th2 ratio. More importantly, administration of Ta1 blocked lipopolysaccharide-induced impairment of hippocampal neurogenesis in early life. These findings suggest that peripheral Ta1 contributes to neurogenesis and cognition probably through a systemic Th1 bias, as well as neuroprotection against LPS infection by Ta1.展开更多
基金the National Natural Science Foundation of China,No. 30700436
文摘In the present study we injected colchicine into the lateral ventricle of Sprague-Dawley rats to investigate the effects of colchicine on the number of different-type neurons in the basal forebrain and to search for neurons resistant to injury. After colchicine injection, the number of nestin^＋ cholinergic neurons was decreased at 1 day, but increased at 3 days and peaked at 14-28 days. The quantity of nestincholinergic neurons, parvalbumin-positive neurons and choline acetyl transferase-positive neurons decreased gradually. Our results indicate that nestin^＋ cholinergic neurons possess better tolerance to colchicine-induced neurotoxicity.
基金supported by the Natural Science Foundation of Guangdong Province, China (2014A030310343, 2015A030313153, and 2016A030313253)the Medical Scientific Research Foundation of Guangdong Province, China (A2015382)the Doctoral Program of Guangzhou Medical University, China (2014C19)
文摘In early life, the immune system plays an essential role in brain development. In our study, the immunopotentiator thymosin alpha-1（Ta1） was peripherally administered to neonatal mice to explore whether the peripheral immunopotentiator affects neurodevelopment and cognition, and to further investigate the relevant mechanism. Compared with the control group, the Ta1 mice displayed better cognitive abilities in early life. The numbers of 5-bromodeoxyuridine（Brd U）＋, nestin＋,T-box transcription factor 2（Tbr2）＋, Brd U＋/doublecortin（DCX）＋, Brd U＋/ionized calcium-binding adaptor molecule 1（Iba1）＋, and Brd U＋/neuronal nuclei（Neu N）＋ cells in the hippocampus were increased in the Ta1 group,accompanied by increased interleukin-4（IL-4）, interferon-gamma, brain-derived neurotrophic factor, nerve growth factor, and insulin-like growth factor-1 as well as decreased IL-6 and tumor necrosis factor-a. Furthermore, the Ta1-group showed a Th1-polarized immune response, and the neurotrophic factors were positively associated with the Th1/Th2 ratio. More importantly, administration of Ta1 blocked lipopolysaccharide-induced impairment of hippocampal neurogenesis in early life. These findings suggest that peripheral Ta1 contributes to neurogenesis and cognition probably through a systemic Th1 bias, as well as neuroprotection against LPS infection by Ta1.
