Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelera...Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.展开更多
Objectives:To investigate the health-related quality of life(HRQL)of long-term survivors of inoperable esophageal squamous cell carcinoma(ESCC)treated with definitive radiation therapy,the real-world trends in the use...Objectives:To investigate the health-related quality of life(HRQL)of long-term survivors of inoperable esophageal squamous cell carcinoma(ESCC)treated with definitive radiation therapy,the real-world trends in the use of advanced radiation techniques,and their impact on the survival outcomes of ESCC patients.Methods:In this multicenter retrospective observational study,the medical records related to demographics and treatment of ESCC patients who were treated with definitive radiation therapy at 14 provincial hospitals in China from 1 January 2015 to 31 December 2016 were analyzed.A HRQL questionnaire was completed by survivors and collected by doctors at the final follow-up.The difference in quality of life between patients with or without recurrence was compared using the Wilcoxon-Mann-Whitney test.Overall survival(OS)was estimated using the Kaplan-Meier method and the group differences were assessed by unstratified log-rank test.The Cox proportional hazards model with Efron’s method of tie handling was used to calculate the risk factors for OS.Results:The data of a total of 3,308 patients were collected for this study,248 were excluded because of missing data,and a final of 3,060 patients were included in the analysis.Most patients(2,901;94.8%)received intensity-modulated radiotherapy(IMRT)/volumetric-modulated arc therapy(VMAT)/tomotherapy(TOMO).The 5-year OS rate was 30%.Patients who received either two-dimensional radiotherapy(2DRT;HR,2.43[95%CI,1.70-3.47];P<0.001)or three-dimensional radiotherapy(3DRT;HR,1.45[95%CI,1.14-1.84];P=0.003)had a significantly increased risk of death compared to those who received IMRT/VMAT/TOMO.Of the 716(23.4%)long-term survivors who completed the HRQL questionnaire,nearly 70%patients were still able to swallow normally or almost normally,and>80%patients did not experience weight loss.Nearly 80%patients found life very enjoyable or were fairly enjoying life.Conclusions:This large,multicenter retrospective study on ESCC patients who received definitive radiation ther-apy found that most ESCC survivors are satisfied with their quality of life.Most patients received advanced radiation technology.Patients who received either 2DRT or 3DRT had a significantly increased risk of death compared to those who received advanced radiation technology.展开更多
Background:Carbohydrate drinking 2-3 hours before surgery has been widely adopted in colorectal operations.However,there is lit-tle direct evidence regarding its application in gastric cancer surgery.We aimed to evalu...Background:Carbohydrate drinking 2-3 hours before surgery has been widely adopted in colorectal operations.However,there is lit-tle direct evidence regarding its application in gastric cancer surgery.We aimed to evaluate the gastric residual volume,safety,and effectiveness of drinking 250 mL of 5%glucose solution 2-3 hours before elective gastric cancer surgery.Methods:We conducted an investigator-initiated,multicenter,randomized-controlled,parallel group,and equivalence trial.Eighty-eight patients with gastric adenocarcinoma were randomized into study or control group.Patients in the control group followed the traditional routine of 6-8 hours preoperative fasting,while those in the study group drank 250 mL of 5%glucose solution 2-3 hours be-fore surgery.Immediately following tracheal intubation,gastric contents were aspirated through gastroscopy.The primary outcome was preoperative gastric residual volume.Results:Eighty-three patients were eventually analysed in the study(42 in the study group and 41 in the control group).Two groups were comparable at baseline characteristics.There were no statistical differences in residual gastric fluid volumes(35.86±27.13 vs 27.70±20.37 mL,P=0.135)and pH values(2.81±1.99 vs 2.66±1.68,P=0.708)between the two groups.Preoperative discomfort was significantly more decreased in the study group than in the control group(thirst score:1.49±1.23 vs 4.14±2.07,P<0.001;hunger score:1.66±1.18 vs 3.00±2.32,P=0.007).There was no statistical difference in the incidence of postoperative complications(19.05%vs 17.07%,P=0.815).Conclusions:Drinking 250 mL of 5%glucose solution 2-3 hours before surgery in elective gastric cancer patients shows benefits in lowering thirst and hunger scores without increasing gastric residual volume and perioperative complications.展开更多
This paper proposes an inverse method for improving the prediction of tunnel displacements during adjacent excavation.In this framework,staged data assimilation and parameter identification are conducted using the mul...This paper proposes an inverse method for improving the prediction of tunnel displacements during adjacent excavation.In this framework,staged data assimilation and parameter identification are conducted using the multi-objective particle swarm optimization algorithm.Recent monitoring data are assumed to be more informative and assigned more weights in the multi-objective optimization to improve the prediction accuracy.Then,an empirical formula is applied to correct the time effect of tunnel displacement.The Kriging method is introduced to surrogate the finite element model to reduce computational cost.The proposed framework is verified using a typical staged“excavation nearing tunnel”case.The predictions using the updated parameters are in good agreement with the measurements.The identified values of underlying soil parameters are within the typical ranges for the unloading condition.The updated time effect indicates that tunnel displacements may develop excessively in the three months after the region S1-B is excavated to the bottom.The maximum vertical tunnel displacement may increase from the currently measured 12 mm to the calculated 26 mm if the later construction is suspended long enough.Subsequent constructions need to be timely conducted to restrain the time effect and control tunnel displacements.展开更多
基金supported by National Natural Science Foundation of China(Grant Nos.32072614 and 31972452)Shandong Provincial Natural Science Foundation(Grant Nos.ZR2020MC146 and ZR2020QC160)Seed improvement project of Shandong Province(Grant No.2020LZGC011-1-4)。
文摘Tree peony(Paeonia suffruticosa Andrews)is a well-known ornamental plant with high economic value,but the short fluorescence is a key obstacle to its ornamental value and industry development.High temperature accelerates flower senescence and abscission,but the associated mechanisms are poorly understood.In this study,the tandem mass tag(TMT)proteome and label-free quantitative ubiquitome from tree peony cut flowers treated with 20℃for 0 h(RT0),20℃or 28℃for 60 h(RT60 or HT60)were examined based on morphological observation,respectively.Totally,6970 proteins and 1545 lysine ubiquitinated(Kub)sites in 844 proteins were identified.Hydrophilic residues(such as glutamate and aspartate)neighboring the Kub sites were in preference,and 36.01%of the Kub sites were located on the protein surface.The differentially expressed proteins(DEPs)and Kub-DEPs in HT60 vs RT60 were mainly enriched in ribosomal protein,protein biosynthesis,secondary metabolites biosynthesis,flavonoid metabolism,carbohydrate catabolism,and auxin biosynthesis and signaling revealed by GO and KEGG analysis,accompanying the increase of endogenous abscisic acid(ABA)accumulation and decrease of endogenous indoleacetic acid(IAA)level.Additionally,the expression patterns of six enzymes(SAMS,ACO,YUC,CHS,ANS and PFK)putatively with Kub modifications were analyzed by proteome and real-time quantitative RT-PCR.The cell-free degradation assays showed PsSAMS and PsACO proteins could be degraded via the 26 S proteasome system in tree peony flowers.Finally,a working model was proposed for the acceleration of flower senescence and abscission by high temperature.In summary,all results contributed to understanding the mechanism of flower senescence induced by high temperature and prolonging fluorescence in tree peony.
基金supported by the Sanming Project of Medicine in Shenzhen(grant num-ber:SZSM201612063)National Natural Science Foundation of China Research(grant numbers:U22A20326,81872454,21441010)the Beijing Xisike Clinical Oncology Research Foundation(grant number:Y-MSD2020-0324).
文摘Objectives:To investigate the health-related quality of life(HRQL)of long-term survivors of inoperable esophageal squamous cell carcinoma(ESCC)treated with definitive radiation therapy,the real-world trends in the use of advanced radiation techniques,and their impact on the survival outcomes of ESCC patients.Methods:In this multicenter retrospective observational study,the medical records related to demographics and treatment of ESCC patients who were treated with definitive radiation therapy at 14 provincial hospitals in China from 1 January 2015 to 31 December 2016 were analyzed.A HRQL questionnaire was completed by survivors and collected by doctors at the final follow-up.The difference in quality of life between patients with or without recurrence was compared using the Wilcoxon-Mann-Whitney test.Overall survival(OS)was estimated using the Kaplan-Meier method and the group differences were assessed by unstratified log-rank test.The Cox proportional hazards model with Efron’s method of tie handling was used to calculate the risk factors for OS.Results:The data of a total of 3,308 patients were collected for this study,248 were excluded because of missing data,and a final of 3,060 patients were included in the analysis.Most patients(2,901;94.8%)received intensity-modulated radiotherapy(IMRT)/volumetric-modulated arc therapy(VMAT)/tomotherapy(TOMO).The 5-year OS rate was 30%.Patients who received either two-dimensional radiotherapy(2DRT;HR,2.43[95%CI,1.70-3.47];P<0.001)or three-dimensional radiotherapy(3DRT;HR,1.45[95%CI,1.14-1.84];P=0.003)had a significantly increased risk of death compared to those who received IMRT/VMAT/TOMO.Of the 716(23.4%)long-term survivors who completed the HRQL questionnaire,nearly 70%patients were still able to swallow normally or almost normally,and>80%patients did not experience weight loss.Nearly 80%patients found life very enjoyable or were fairly enjoying life.Conclusions:This large,multicenter retrospective study on ESCC patients who received definitive radiation ther-apy found that most ESCC survivors are satisfied with their quality of life.Most patients received advanced radiation technology.Patients who received either 2DRT or 3DRT had a significantly increased risk of death compared to those who received advanced radiation technology.
基金supported by the National Natural Science Foundation of China[grant numbers 82172637 and 81702319]the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences[grant number 2021-JKCS-004]+1 种基金the Guangdong Provincial Key Laboratory of Digestive Cancer Research[grant number 2021B1212040006]Research startup fund of part-time PI,SAHSYSU[grant number ZSQYJZPI202010].
文摘Background:Carbohydrate drinking 2-3 hours before surgery has been widely adopted in colorectal operations.However,there is lit-tle direct evidence regarding its application in gastric cancer surgery.We aimed to evaluate the gastric residual volume,safety,and effectiveness of drinking 250 mL of 5%glucose solution 2-3 hours before elective gastric cancer surgery.Methods:We conducted an investigator-initiated,multicenter,randomized-controlled,parallel group,and equivalence trial.Eighty-eight patients with gastric adenocarcinoma were randomized into study or control group.Patients in the control group followed the traditional routine of 6-8 hours preoperative fasting,while those in the study group drank 250 mL of 5%glucose solution 2-3 hours be-fore surgery.Immediately following tracheal intubation,gastric contents were aspirated through gastroscopy.The primary outcome was preoperative gastric residual volume.Results:Eighty-three patients were eventually analysed in the study(42 in the study group and 41 in the control group).Two groups were comparable at baseline characteristics.There were no statistical differences in residual gastric fluid volumes(35.86±27.13 vs 27.70±20.37 mL,P=0.135)and pH values(2.81±1.99 vs 2.66±1.68,P=0.708)between the two groups.Preoperative discomfort was significantly more decreased in the study group than in the control group(thirst score:1.49±1.23 vs 4.14±2.07,P<0.001;hunger score:1.66±1.18 vs 3.00±2.32,P=0.007).There was no statistical difference in the incidence of postoperative complications(19.05%vs 17.07%,P=0.815).Conclusions:Drinking 250 mL of 5%glucose solution 2-3 hours before surgery in elective gastric cancer patients shows benefits in lowering thirst and hunger scores without increasing gastric residual volume and perioperative complications.
基金supported by the National Key Research and Development Program of China(Grant Nos.2017YFE0119500 and 2016YFC0800200)National Natural Science Foundation of China(Grant Nos.51620105008,52078464,and U2006225)the program of the China Scholarships Scholarship Council(No.202006320256).
文摘This paper proposes an inverse method for improving the prediction of tunnel displacements during adjacent excavation.In this framework,staged data assimilation and parameter identification are conducted using the multi-objective particle swarm optimization algorithm.Recent monitoring data are assumed to be more informative and assigned more weights in the multi-objective optimization to improve the prediction accuracy.Then,an empirical formula is applied to correct the time effect of tunnel displacement.The Kriging method is introduced to surrogate the finite element model to reduce computational cost.The proposed framework is verified using a typical staged“excavation nearing tunnel”case.The predictions using the updated parameters are in good agreement with the measurements.The identified values of underlying soil parameters are within the typical ranges for the unloading condition.The updated time effect indicates that tunnel displacements may develop excessively in the three months after the region S1-B is excavated to the bottom.The maximum vertical tunnel displacement may increase from the currently measured 12 mm to the calculated 26 mm if the later construction is suspended long enough.Subsequent constructions need to be timely conducted to restrain the time effect and control tunnel displacements.
