Perovskite solar cells(PSCs)have emerged as a promising photovoltaic technology because of their high light absorption coefficient,long carrier diffusion distance,and tunable bandgap.However,PSCs face challenges such ...Perovskite solar cells(PSCs)have emerged as a promising photovoltaic technology because of their high light absorption coefficient,long carrier diffusion distance,and tunable bandgap.However,PSCs face challenges such as hysteresis effects and stability issues.In this study,we introduced a novel approach to improve film crystallization by leveraging 4-tert-butylpyridine(TBP)molecules,thereby enhancing the performance and stability of PSCs.Our findings demonstrate the effective removal of PbI_(2)from the perovskite surface through strong coordination with TBP molecules.Additionally,by carefully adjusting the concentration of the TBP solution,we achieved enhanced film crystallinity without disrupting the perovskite structure.The TBP-treated perovskite films exhibit a low defect density,improved crystallinity,and improved carrier lifetime.As a result,the PSCs manufactured with TBP treatment achieve power conversion efficiency(PCE)exceeding 24%.Moreover,we obtained the PCE of 21.39%for the 12.25 cm^(2)module.展开更多
A simple and sensitive high performance liquid chromatography with fluorescence detection （HPLC-FD） has been developed for simultaneous quantification of doxorubicin （DOX） and its dipeptide conjugate prodrug （PDO...A simple and sensitive high performance liquid chromatography with fluorescence detection （HPLC-FD） has been developed for simultaneous quantification of doxorubicin （DOX） and its dipeptide conjugate prodrug （PDOX） in mice plasma. The chromatographic separation was carried out on an Amethyst C18-H column with gradient mobile phase of 0.1% formic acid and 0.1% formic acid in acetonitrile at a flow rate of 1.0 mL/min. The excitation and emission wavelengths were set at 490 and 550 nm, respectively. The method was comprehensively validated. The limits of detection were low up to 5.0 ng/mL for DOX and 25.0 ng/mL for PDOX. And the limits of quantification were low up to 12.5 ng/mL for DOX and 50 ng/mL for PDOX, which were lower than those for most of the current methods. The calibration curves showed good linearity （R2 〉 0.999） over the concentration ranges. The extraction recoveries ranged from 84.0% to 88.2% for DOX and from 85.4% to 89.2% for PDOX. Satisfactory intra-day and inter-day precisions were achieved with RSDs less than 9.1%. The results show that the developed HPLC-FD method is accurate, reliable and will be helpful for preclinical pharmacokinetic study of DOX and PDOX.展开更多
Sepsis,a highly life-threatening organ dysfunction caused by uncontrollable immune responses to infection,is a leading contributor to mortality in intensive care units.Sepsis-related deaths have been reported to accou...Sepsis,a highly life-threatening organ dysfunction caused by uncontrollable immune responses to infection,is a leading contributor to mortality in intensive care units.Sepsis-related deaths have been reported to account for 19.7%of all global deaths.However,no effective and specific therapeutic for clinical sepsis management is available due to the complex pathogenesis.Concurrently eliminating infections and restoring immune homeostasis are regarded as the core strategies to manage sepsis.Sophisticated nanoplatforms guided by supramolecular and medicinal chemistry,targeting infection and/or imbalanced immune responses,have emerged as potent tools to combat sepsis by supporting more accurate diagnosis and precision treatment.Nanoplatforms can overcome the barriers faced by clinical strategies,including delayed diagnosis,drug resistance and incapacity to manage immune disorders.Here,we present a comprehensive review highlighting the pathogenetic characteristics of sepsis and future therapeutic concepts,summarizing the progress of these well-designed nanoplatforms in sepsis management and discussing the ongoing challenges and perspectives regarding future potential therapies.Based on these state-of-the-art studies,this review will advance multidisciplinary collaboration and drive clinical translation to remedy sepsis.展开更多
In view of artificially high urbanization rate of China and possible causes,survey and data analysis methods were used to discuss reform of household registration system,and path for promoting rural land system reform...In view of artificially high urbanization rate of China and possible causes,survey and data analysis methods were used to discuss reform of household registration system,and path for promoting rural land system reform. Results show that it is required to eliminate bottlenecks of land system in the advance of new urbanization process. In line with land system bottlenecks of new urbanization,it is recommended to take following measures.( i) Promoting land right confirmation centered land system reform.( ii) Weakening rural land ownership and stressing land use right.( iii) Establishing urban and rural integrated land market and putting an end to low cost urbanization road.( iv) Realizing asset value of rural construction land and tackling the problem of insufficient construction land.( v) Reforming the land expropriation system and increasing the share of gain in land value to farmers.( vi) Implementing different land policies to promote coordinated development of regional social economy.( vii) Establishing land circulation access system to ensure unchanged land use purpose and high efficient use.展开更多
Multisensory lab based in Peking University,has carried out basic studies in multisensory space and time processing,intersensory binding and haptic/tactile perception.We exploited a typical paradigm of multisensory il...Multisensory lab based in Peking University,has carried out basic studies in multisensory space and time processing,intersensory binding and haptic/tactile perception.We exploited a typical paradigm of multisensory illusion-temporal ventriloquist effect and applied it in a wide range of multisensory interactions(mainly focused on temporal processing).In this work,we summarized how the tactile stimuli were exploited to compose tactile cues and as tactile apparent motion to interface with other sensory stimuli(visual and auditory stimuli)to examine the underlying perceptual organization in a multisensory context.Moreover,we introduced two examples of wearable haptic/tactile perception in our lab,by using two customized tactile devices and discussed the potential applications in this field.展开更多
Mitochondrial flash(mitoflash)is the electrochemical excitation of a single mitochondrion and plays diverse signaling roles in a variety of cells.From the viewpoint of bioenergetics,it represents the transient uncoupl...Mitochondrial flash(mitoflash)is the electrochemical excitation of a single mitochondrion and plays diverse signaling roles in a variety of cells.From the viewpoint of bioenergetics,it represents the transient uncoupling of mitochondrial respiration from ATP synthesis.Brown adipose tissue(BAT)produces heat,primarily via mitochondrial uncoupled respiration.Here,we aim to illustrate the mitoflash activity in BAT thermogenesis.We show that BAT mitochondria undergo stochastic and intermittent mitoflashes at the levels of isolated mitochondria,cultured brown adipocytes,and even intact BAT.A BAT mitoflash contains multiple signals,including transient depolarization of mitochondrial membrane potential,transient matrix alkalization,and bursting production of reactive oxygen species.Importantly,thermogenic activation by b3-adrenergic stimulation dramatically augments mitoflash incidence,with mild effects on its unitary characteristics.Ablation of the thermogenic uncoupling protein 1(UCP1)showed little effect on mitoflash biogenesis during heat production.Collectively,our results suggest that mitoflash might constitute an elemental signaling activity of mitochondria to regulate BAT thermogenesis.展开更多
Diffusion-weighted imaging(DWI) is considered to be one of the dominant modalities used in prostate cancer(PCa) detection and the assessment of lesion aggressiveness,especially for peripheral zone(PZ) PCa.Computer-aid...Diffusion-weighted imaging(DWI) is considered to be one of the dominant modalities used in prostate cancer(PCa) detection and the assessment of lesion aggressiveness,especially for peripheral zone(PZ) PCa.Computer-aided diagnosis(CAD),which is capable of automatically extracting and evaluating image features,can integrate multiple parameters and improve the detection of PCa.In this study,13 quantitative image features were extracted from DWI by CAD,and diagnostic efficacy was analyzed in both the PZ and transition zone(TZ).The results demonstrated that there was a significant difference(P<0.05) between PCa and non-PCa for nine of the 13 features in the PZ and five of the 13 features in the TZ.Besides,the prediction outcome of CAD had a strong correlation with the DWI scores that were graded by experienced radiologists according to the Prostate Imaging-Reporting and Data System Version 2(PI-RADS v2).展开更多
The Taihu Lake Basin, an east-coastal developed area, is one of the fastest-growing metropolitan areas in China. Ecosystem services in the Taihu Lake Basin have been overexploited and jeopardized. Based on land-use an...The Taihu Lake Basin, an east-coastal developed area, is one of the fastest-growing metropolitan areas in China. Ecosystem services in the Taihu Lake Basin have been overexploited and jeopardized. Based on land-use and land-cover change （LUCC） data from 1979, 1984, 2000, and 2010, in conjunction with the adjusted ecosystem service values （ESV）, changes in ESV were analyzed in detail. Results revealed that LUCC resulted in a substantial decrease in total ESV from S3.92 billion in 1979 to S2.98 billion in 2010. The ESV of cropland decreased from $1.64 billion in 1979 to S1.34 billion in 2010, which represented a 20.28% reduction. The ESV of water areas decreased from S1.08 billion in 1979 to $0.36 billion in 2010, which represented a 65.62% reduction mainly because of a decline in water quality. In terms of annual change rate, cropland and water areas showed a sustained downward trend. Spatially, ESV declines were mainly observed in Suzhou, Wuxi, Changzhou, and Shanghai, probably due to a combination of economic progress, population growth, and rapid urbanization. The research results can be a useful reference for policymakers in mitigating ESV decline.展开更多
Previous studies revealed that extracellular regulated kinase-1 and -2 （ERK1/2） cascade plays pivotal roles in regulating oocyte meiotic cell cycle progression. However, most knowledge about the in vivo function of ...Previous studies revealed that extracellular regulated kinase-1 and -2 （ERK1/2） cascade plays pivotal roles in regulating oocyte meiotic cell cycle progression. However, most knowledge about the in vivo function of ERK1/2 in mammalian oocytes was indirectly obtained from analyzing the phenotypes of Mos knockout mice. In this study, we knocked out Erkl and Erk2 in mouse oocytes as early as the primordial follicle stage using the well-characterized Gdp-Cre mouse model, and for the first time directly investigated the in vivo function of ERK1/2 in mouse oocytes. In this novel mouse model, we observed that ERK1/2 activities in oocyte are dispensable for primordial follicle maintenance, activation and follicle growth. Different from the Mos null oocytes, the ERK1/2-deleted oocytes had well-assembled spindles at metaphase I （MI）, extruded polar body-I （PB1） with normal sizes, and did not undergo a full parthenogenetic activation characterized for pronuclear formation. However, the ovulated ERK1/2-deleted oocytes had poorly-assembled metaphase II （MII） spindles, spontaneously released polar body-2 （PB2）, and were arrested at another metaphase called metaphase III （MIII）. In addition, ERK1/2 deletion prevented male pronuclear formation after fertilization, and caused female infertility. In conclusion, these results indicate that ERK1/2 activities are required for not only MII-arrest maintenance, but also efficient pronuclear formation in mouse oocytes.展开更多
Mesenchymal stem cells(MSCs) are important cell sources in cartilage tissue development and homeostasis,and multiple strategies have been developed to improve MSCs chondrogenic differentiation with an aim of promoting...Mesenchymal stem cells(MSCs) are important cell sources in cartilage tissue development and homeostasis,and multiple strategies have been developed to improve MSCs chondrogenic differentiation with an aim of promoting cartilage regeneration.Here we report the effects of combining nanosecond pulsed electric fields(ns PEFs) followed by treatment with ghrelin(a hormone that stimulates release of growth hormone) to regulate chondrogenesis of MSCs.ns PEFs and ghrelin were observed to separately enhance the chondrogenesis of MSCs,and the effects were significantly enhanced when the bioelectric stimulation and hormone were combined,which in turn improved osteochondral tissue repair of these cells within Sprague Dawley rats.We further found that ns PEFs can prime MSCs to be more receptive to subsequent stimuli of differentiation by upregulated Oct4/Nanog and activated JNK signaling pathway.Ghrelin initiated chondrogenic differentiation by activation of ERK1/2 signaling pathway,and RNA-seq results indicated 243 genes were regulated,and JAK-STAT signaling pathway was involved.Interestingly,the sequential order of applying these two stimuli is critical,with ns PEFs pretreatment followed by ghrelin enhanced chondrogenesis of MSCs in vitro and subsequent cartilage regeneration in vivo,but not vice versa.This synergistic prochondrogenic effects provide us new insights and strategies for future cell-based therapies.展开更多
Patients with chronic kidney disease are at high risk of hyperkalemia that is associated with various lifethreatening complications.Treatments primarily rely on orally administered potassium binding agents,along with ...Patients with chronic kidney disease are at high risk of hyperkalemia that is associated with various lifethreatening complications.Treatments primarily rely on orally administered potassium binding agents,along with low curative effects and various side effects.Herein,direct serum potassium uptake was realized via zeolite–heparin-mimicking-polymer hybrid microbeads.The preparation process involved the synthesis of the heparin-mimicking polymer via the in situ cross-linking polymerization of acrylic acid and N-vinylpyrrolidone in polyethersulfone solution,the fabrication of microbeads via zeolite-mixing,electro-spraying and phase-inversion,and the subsequent aqueous-phase modifications based on ion-exchange and metal-leaching.An ultra-high(about 88%)amount of zeolite could be incorporated and well locked inside the polymer matrix.Potassium uptake capability was verified in water,normal saline and human serum,showing high selectivity and fast adsorption.The microbeads exhibited satisfying blood compatibility,negligible hemolysis ratio,prolonged clotting time,inhibited contact activation,and enhanced antifouling property toward serum proteins and cells.The proposed approach toward zeolite–heparin-mimicking-polymer hybrid microbeads provided a cheap,efficient and safe treatment protocol of hyperkalemia for the high-risk patients.展开更多
The growing demand for brilliant smile and tooth whitening has led to the increasing significance of tooth whitening methods.However,the development of tooth whitening materials with high efficiency and safety has bee...The growing demand for brilliant smile and tooth whitening has led to the increasing significance of tooth whitening methods.However,the development of tooth whitening materials with high efficiency and safety has been considered to be an urgent challenge.Here,we report a novel tooth whitening strategy,which overcomes the limitation of conventional methods,shows excellent tooth whitening efficiency and safety,and even exhibits outstanding antibacterial activity.Inspired by stomata and chloroplasts in green leaves,we have developed an integrated two-phase free radical hydrogel(TP-GEL),which integrates solid-phase and liquid-phase free radical functional components fabricated by cold plasma technology.TP-GEL demonstrated safe and ultrafast tooth-whitening effect with or without visible light,which can be more significant than that of 30% H_(2)O_(2),the commonly whitening material in dental clinical practice.More importantly,we also avoid the damage to enamel,such as microhardness,roughness,surface morphology.Benefiting from free radicals,much higher antibacterial properties than commercial products(Chlorhexidine)was obtained.This research provides fundamental design criteria for the development of dual functional oral health care materials with advanced tooth whitening and antibacterial activity.展开更多
To the Editor:Multiple myeloma(MM),the second most common hematological malignancy,is caused by clonal proliferation of plasma cells and presents characteristics including anemia,bone pain,renal insufficiency,and hype...To the Editor:Multiple myeloma(MM),the second most common hematological malignancy,is caused by clonal proliferation of plasma cells and presents characteristics including anemia,bone pain,renal insufficiency,and hypercalcemia.The adhesion of myeloma cells to the bone marrow(BM)microenvironment results in the mainly intramedullary involvement of MM,and sometimes,however,plasma cells can disseminate and infiltrate into extramedullary(EM)sites.展开更多
The abdomen contains many organs, along with a range of abdominal diseases that require accurate diagnosis. Clinical imaging plays a crucial role in abdominal disease diagnosis, prognosis, and treatment assessment. Fo...The abdomen contains many organs, along with a range of abdominal diseases that require accurate diagnosis. Clinical imaging plays a crucial role in abdominal disease diagnosis, prognosis, and treatment assessment. For decades, physicists have focused on innovation in terms of imaging techniques, assisting radiologists to improve abdominal diseases detection and diagnosis. Consequently, many new imaging methods for abdominal organs have been developed, including computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), X-ray, endoscopy, and ultrasound (McAuliffe et al., 2001;Dhawan et al., 2011). Each imaging method has its own unique features. For example, abdominal MRI can achieve the most comprehensive assessment of abdominal lesions, such as the liver, kidney and prostate (Yang et al., 2017;Wang et al., 2018;Gao et al., 2017) mainly due to its diversiform sequences, including structural imaging such as T1 weighted imaging and T2 weighted imaging, as well as diffusion imaging, perfusion imaging, arterial spin labeling and other functional imaging that reflect different functional changes.展开更多
基金financial support from various entities,including the Foundation of Anhui Science and Technology University[HCYJ202201]the Anhui Science and Technology University’s Student Innovation and Entrepreneurship Training Program[S202310879115,202310879053]+4 种基金the Key Project of Natural Science Research in Anhui Science and Technology University[2021ZRZD07]the Chuzhou Science and Technology Project[2021GJ002]the Anhui Province Key Research and Development Program[202304a05020085]the Natural Science Research Project of Anhui Educational Committee[2023AH051877]The Opening Project of State Key Laboratory of Advanced Technology for Float Glass[2020KF06,2022KF06]。
文摘Perovskite solar cells(PSCs)have emerged as a promising photovoltaic technology because of their high light absorption coefficient,long carrier diffusion distance,and tunable bandgap.However,PSCs face challenges such as hysteresis effects and stability issues.In this study,we introduced a novel approach to improve film crystallization by leveraging 4-tert-butylpyridine(TBP)molecules,thereby enhancing the performance and stability of PSCs.Our findings demonstrate the effective removal of PbI_(2)from the perovskite surface through strong coordination with TBP molecules.Additionally,by carefully adjusting the concentration of the TBP solution,we achieved enhanced film crystallinity without disrupting the perovskite structure.The TBP-treated perovskite films exhibit a low defect density,improved crystallinity,and improved carrier lifetime.As a result,the PSCs manufactured with TBP treatment achieve power conversion efficiency(PCE)exceeding 24%.Moreover,we obtained the PCE of 21.39%for the 12.25 cm^(2)module.
基金supported by the National Natural Science Foundation of China (Grant nos. 21375101, 81573384 and 91417301)Natural Science Foundation of Hubei Province, China (No. 2014CFA077)Innovation Seed Fund and Translational Medical Research Fund of Wuhan University School of Medicine, China
文摘A simple and sensitive high performance liquid chromatography with fluorescence detection （HPLC-FD） has been developed for simultaneous quantification of doxorubicin （DOX） and its dipeptide conjugate prodrug （PDOX） in mice plasma. The chromatographic separation was carried out on an Amethyst C18-H column with gradient mobile phase of 0.1% formic acid and 0.1% formic acid in acetonitrile at a flow rate of 1.0 mL/min. The excitation and emission wavelengths were set at 490 and 550 nm, respectively. The method was comprehensively validated. The limits of detection were low up to 5.0 ng/mL for DOX and 25.0 ng/mL for PDOX. And the limits of quantification were low up to 12.5 ng/mL for DOX and 50 ng/mL for PDOX, which were lower than those for most of the current methods. The calibration curves showed good linearity （R2 〉 0.999） over the concentration ranges. The extraction recoveries ranged from 84.0% to 88.2% for DOX and from 85.4% to 89.2% for PDOX. Satisfactory intra-day and inter-day precisions were achieved with RSDs less than 9.1%. The results show that the developed HPLC-FD method is accurate, reliable and will be helpful for preclinical pharmacokinetic study of DOX and PDOX.
基金the National Key Research and Development Program of China(Grant No.2018YFC2001904).
文摘Sepsis,a highly life-threatening organ dysfunction caused by uncontrollable immune responses to infection,is a leading contributor to mortality in intensive care units.Sepsis-related deaths have been reported to account for 19.7%of all global deaths.However,no effective and specific therapeutic for clinical sepsis management is available due to the complex pathogenesis.Concurrently eliminating infections and restoring immune homeostasis are regarded as the core strategies to manage sepsis.Sophisticated nanoplatforms guided by supramolecular and medicinal chemistry,targeting infection and/or imbalanced immune responses,have emerged as potent tools to combat sepsis by supporting more accurate diagnosis and precision treatment.Nanoplatforms can overcome the barriers faced by clinical strategies,including delayed diagnosis,drug resistance and incapacity to manage immune disorders.Here,we present a comprehensive review highlighting the pathogenetic characteristics of sepsis and future therapeutic concepts,summarizing the progress of these well-designed nanoplatforms in sepsis management and discussing the ongoing challenges and perspectives regarding future potential therapies.Based on these state-of-the-art studies,this review will advance multidisciplinary collaboration and drive clinical translation to remedy sepsis.
基金Supported by Project of Anhui Development and Reform Commission "Study on Reform and Innovation of Rural Land System in Anhui Province"(20141121)
文摘In view of artificially high urbanization rate of China and possible causes,survey and data analysis methods were used to discuss reform of household registration system,and path for promoting rural land system reform. Results show that it is required to eliminate bottlenecks of land system in the advance of new urbanization process. In line with land system bottlenecks of new urbanization,it is recommended to take following measures.( i) Promoting land right confirmation centered land system reform.( ii) Weakening rural land ownership and stressing land use right.( iii) Establishing urban and rural integrated land market and putting an end to low cost urbanization road.( iv) Realizing asset value of rural construction land and tackling the problem of insufficient construction land.( v) Reforming the land expropriation system and increasing the share of gain in land value to farmers.( vi) Implementing different land policies to promote coordinated development of regional social economy.( vii) Establishing land circulation access system to ensure unchanged land use purpose and high efficient use.
基金Supported by Natural Science Foundation of China(NSFC61527804)NSFC and the German Research Foundation(DFG)in Project Crossmodal Learning(NSFC 61621136008/DFG TRR-169)Research fund from brain lab,TAL education group,China.
文摘Multisensory lab based in Peking University,has carried out basic studies in multisensory space and time processing,intersensory binding and haptic/tactile perception.We exploited a typical paradigm of multisensory illusion-temporal ventriloquist effect and applied it in a wide range of multisensory interactions(mainly focused on temporal processing).In this work,we summarized how the tactile stimuli were exploited to compose tactile cues and as tactile apparent motion to interface with other sensory stimuli(visual and auditory stimuli)to examine the underlying perceptual organization in a multisensory context.Moreover,we introduced two examples of wearable haptic/tactile perception in our lab,by using two customized tactile devices and discussed the potential applications in this field.
基金supported by the National Natural Science Foundation of China(31971158,92157105,and 32293210)the National Science and Technology Innovation 2030Major Program(2022ZD0211900)CAMS Innovation Fund for Medical Sciences(2019-I2M-5–054).
文摘Mitochondrial flash(mitoflash)is the electrochemical excitation of a single mitochondrion and plays diverse signaling roles in a variety of cells.From the viewpoint of bioenergetics,it represents the transient uncoupling of mitochondrial respiration from ATP synthesis.Brown adipose tissue(BAT)produces heat,primarily via mitochondrial uncoupled respiration.Here,we aim to illustrate the mitoflash activity in BAT thermogenesis.We show that BAT mitochondria undergo stochastic and intermittent mitoflashes at the levels of isolated mitochondria,cultured brown adipocytes,and even intact BAT.A BAT mitoflash contains multiple signals,including transient depolarization of mitochondrial membrane potential,transient matrix alkalization,and bursting production of reactive oxygen species.Importantly,thermogenic activation by b3-adrenergic stimulation dramatically augments mitoflash incidence,with mild effects on its unitary characteristics.Ablation of the thermogenic uncoupling protein 1(UCP1)showed little effect on mitoflash biogenesis during heat production.Collectively,our results suggest that mitoflash might constitute an elemental signaling activity of mitochondria to regulate BAT thermogenesis.
文摘Diffusion-weighted imaging(DWI) is considered to be one of the dominant modalities used in prostate cancer(PCa) detection and the assessment of lesion aggressiveness,especially for peripheral zone(PZ) PCa.Computer-aided diagnosis(CAD),which is capable of automatically extracting and evaluating image features,can integrate multiple parameters and improve the detection of PCa.In this study,13 quantitative image features were extracted from DWI by CAD,and diagnostic efficacy was analyzed in both the PZ and transition zone(TZ).The results demonstrated that there was a significant difference(P<0.05) between PCa and non-PCa for nine of the 13 features in the PZ and five of the 13 features in the TZ.Besides,the prediction outcome of CAD had a strong correlation with the DWI scores that were graded by experienced radiologists according to the Prostate Imaging-Reporting and Data System Version 2(PI-RADS v2).
文摘The Taihu Lake Basin, an east-coastal developed area, is one of the fastest-growing metropolitan areas in China. Ecosystem services in the Taihu Lake Basin have been overexploited and jeopardized. Based on land-use and land-cover change （LUCC） data from 1979, 1984, 2000, and 2010, in conjunction with the adjusted ecosystem service values （ESV）, changes in ESV were analyzed in detail. Results revealed that LUCC resulted in a substantial decrease in total ESV from S3.92 billion in 1979 to S2.98 billion in 2010. The ESV of cropland decreased from $1.64 billion in 1979 to S1.34 billion in 2010, which represented a 20.28% reduction. The ESV of water areas decreased from S1.08 billion in 1979 to $0.36 billion in 2010, which represented a 65.62% reduction mainly because of a decline in water quality. In terms of annual change rate, cropland and water areas showed a sustained downward trend. Spatially, ESV declines were mainly observed in Suzhou, Wuxi, Changzhou, and Shanghai, probably due to a combination of economic progress, population growth, and rapid urbanization. The research results can be a useful reference for policymakers in mitigating ESV decline.
基金supported by the National Basic Research Program of China (Nos. 2011CB944504 and 2012CB944403)the National Natural Science Foundation of China (Nos. 81172473 and 31371449)
文摘Previous studies revealed that extracellular regulated kinase-1 and -2 （ERK1/2） cascade plays pivotal roles in regulating oocyte meiotic cell cycle progression. However, most knowledge about the in vivo function of ERK1/2 in mammalian oocytes was indirectly obtained from analyzing the phenotypes of Mos knockout mice. In this study, we knocked out Erkl and Erk2 in mouse oocytes as early as the primordial follicle stage using the well-characterized Gdp-Cre mouse model, and for the first time directly investigated the in vivo function of ERK1/2 in mouse oocytes. In this novel mouse model, we observed that ERK1/2 activities in oocyte are dispensable for primordial follicle maintenance, activation and follicle growth. Different from the Mos null oocytes, the ERK1/2-deleted oocytes had well-assembled spindles at metaphase I （MI）, extruded polar body-I （PB1） with normal sizes, and did not undergo a full parthenogenetic activation characterized for pronuclear formation. However, the ovulated ERK1/2-deleted oocytes had poorly-assembled metaphase II （MII） spindles, spontaneously released polar body-2 （PB2）, and were arrested at another metaphase called metaphase III （MIII）. In addition, ERK1/2 deletion prevented male pronuclear formation after fertilization, and caused female infertility. In conclusion, these results indicate that ERK1/2 activities are required for not only MII-arrest maintenance, but also efficient pronuclear formation in mouse oocytes.
基金supported by the National Key Research and Development Program of China (2019YFA0111900)the National Natural Science Foundation of China (81772334)。
文摘Mesenchymal stem cells(MSCs) are important cell sources in cartilage tissue development and homeostasis,and multiple strategies have been developed to improve MSCs chondrogenic differentiation with an aim of promoting cartilage regeneration.Here we report the effects of combining nanosecond pulsed electric fields(ns PEFs) followed by treatment with ghrelin(a hormone that stimulates release of growth hormone) to regulate chondrogenesis of MSCs.ns PEFs and ghrelin were observed to separately enhance the chondrogenesis of MSCs,and the effects were significantly enhanced when the bioelectric stimulation and hormone were combined,which in turn improved osteochondral tissue repair of these cells within Sprague Dawley rats.We further found that ns PEFs can prime MSCs to be more receptive to subsequent stimuli of differentiation by upregulated Oct4/Nanog and activated JNK signaling pathway.Ghrelin initiated chondrogenic differentiation by activation of ERK1/2 signaling pathway,and RNA-seq results indicated 243 genes were regulated,and JAK-STAT signaling pathway was involved.Interestingly,the sequential order of applying these two stimuli is critical,with ns PEFs pretreatment followed by ghrelin enhanced chondrogenesis of MSCs in vitro and subsequent cartilage regeneration in vivo,but not vice versa.This synergistic prochondrogenic effects provide us new insights and strategies for future cell-based therapies.
基金This work was financially sponsored by the State Key Research Development Programme of China(Grant No.2016YFC1103000)the National Natural Science Foundation of China(No.51773127,51873115)+1 种基金Science and Technology Program of Sichuan Province(2019YJ0132)We gratefully acknowledge the help of the Analytical and Testing Center at Sichuan University for the SEM and XPS analysis.
文摘Patients with chronic kidney disease are at high risk of hyperkalemia that is associated with various lifethreatening complications.Treatments primarily rely on orally administered potassium binding agents,along with low curative effects and various side effects.Herein,direct serum potassium uptake was realized via zeolite–heparin-mimicking-polymer hybrid microbeads.The preparation process involved the synthesis of the heparin-mimicking polymer via the in situ cross-linking polymerization of acrylic acid and N-vinylpyrrolidone in polyethersulfone solution,the fabrication of microbeads via zeolite-mixing,electro-spraying and phase-inversion,and the subsequent aqueous-phase modifications based on ion-exchange and metal-leaching.An ultra-high(about 88%)amount of zeolite could be incorporated and well locked inside the polymer matrix.Potassium uptake capability was verified in water,normal saline and human serum,showing high selectivity and fast adsorption.The microbeads exhibited satisfying blood compatibility,negligible hemolysis ratio,prolonged clotting time,inhibited contact activation,and enhanced antifouling property toward serum proteins and cells.The proposed approach toward zeolite–heparin-mimicking-polymer hybrid microbeads provided a cheap,efficient and safe treatment protocol of hyperkalemia for the high-risk patients.
基金financially supported by the Peking University Biomed-X Foundation,China。
文摘The growing demand for brilliant smile and tooth whitening has led to the increasing significance of tooth whitening methods.However,the development of tooth whitening materials with high efficiency and safety has been considered to be an urgent challenge.Here,we report a novel tooth whitening strategy,which overcomes the limitation of conventional methods,shows excellent tooth whitening efficiency and safety,and even exhibits outstanding antibacterial activity.Inspired by stomata and chloroplasts in green leaves,we have developed an integrated two-phase free radical hydrogel(TP-GEL),which integrates solid-phase and liquid-phase free radical functional components fabricated by cold plasma technology.TP-GEL demonstrated safe and ultrafast tooth-whitening effect with or without visible light,which can be more significant than that of 30% H_(2)O_(2),the commonly whitening material in dental clinical practice.More importantly,we also avoid the damage to enamel,such as microhardness,roughness,surface morphology.Benefiting from free radicals,much higher antibacterial properties than commercial products(Chlorhexidine)was obtained.This research provides fundamental design criteria for the development of dual functional oral health care materials with advanced tooth whitening and antibacterial activity.
基金National Natural Science Foundation of China(No. 82070223)Social Development Project of Jiangsu Science and Technology Plan(No. BE2022810)
文摘To the Editor:Multiple myeloma(MM),the second most common hematological malignancy,is caused by clonal proliferation of plasma cells and presents characteristics including anemia,bone pain,renal insufficiency,and hypercalcemia.The adhesion of myeloma cells to the bone marrow(BM)microenvironment results in the mainly intramedullary involvement of MM,and sometimes,however,plasma cells can disseminate and infiltrate into extramedullary(EM)sites.
文摘The abdomen contains many organs, along with a range of abdominal diseases that require accurate diagnosis. Clinical imaging plays a crucial role in abdominal disease diagnosis, prognosis, and treatment assessment. For decades, physicists have focused on innovation in terms of imaging techniques, assisting radiologists to improve abdominal diseases detection and diagnosis. Consequently, many new imaging methods for abdominal organs have been developed, including computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), X-ray, endoscopy, and ultrasound (McAuliffe et al., 2001;Dhawan et al., 2011). Each imaging method has its own unique features. For example, abdominal MRI can achieve the most comprehensive assessment of abdominal lesions, such as the liver, kidney and prostate (Yang et al., 2017;Wang et al., 2018;Gao et al., 2017) mainly due to its diversiform sequences, including structural imaging such as T1 weighted imaging and T2 weighted imaging, as well as diffusion imaging, perfusion imaging, arterial spin labeling and other functional imaging that reflect different functional changes.
