Background:Dihydrolipoamide S-acetyltransferase(DLAT)is a subunit of the pyruvate dehydrogenase complex(PDC),a rate-limiting enzyme complex,that can participate in either glycolysis or the tricarboxylic acid cycle(TCA...Background:Dihydrolipoamide S-acetyltransferase(DLAT)is a subunit of the pyruvate dehydrogenase complex(PDC),a rate-limiting enzyme complex,that can participate in either glycolysis or the tricarboxylic acid cycle(TCA).However,the pathogenesis is not fully understood.We aimed to perform a more systematic and comprehensive analysis of DLAT in the occurrence and progression of tumors,and to investigate its function in patients’prognosis and immunotherapy.Methods:The differential expression,diagnosis,prognosis,genetic and epigenetic alterations,tumor microenvironment,stemness,immune infiltration cells,function enrichment,single-cell analysis,and drug response across cancers were conducted based on multiple computational tools.Additionally,we validated its carcinogenic effect and possible mechanism in glioma cells.Results:We exhibited that DLAT expression was increased in most tumors,especially in glioma,and affected the survival of tumor patients.DLAT was related to RNA modification genes,DNA methylation,immune infiltration,and immune infiltration cells,including CD4+T cells,CD8+T cells,Tregs,and cancer-associatedfibroblasts.Single-cell analysis displayed that DLAT might regulate cancer by mediating angiogenesis,inflammation,and stemness.Enrichment analysis revealed that DLAT might take part in the cell cycle pathway.Increased expression of DLAT leads tumor cells to be more resistant to many kinds of compounds,including PI3Kβinhibitors,PKC inhibitors,HSP90 inhibitors,and MEK inhibitors.In addition,glioma cells with DLAT silence inhibited proliferation,migration,and invasion ability,and promoted cell apoptosis.Conclusion:We conducted a comprehensive analysis of DLAT in the occurrence and progression of tumors,and its possible functions and mechanisms.DLAT is a potential diagnostic,prognostic,and immunotherapeutic biomarker for cancer patients.展开更多
[Objectives]Integrated land productivity can reflect the comprehensive utilization of land and the overall output level,which is the most basic and commonly used indicator in assessing land use efficiency.This thesis ...[Objectives]Integrated land productivity can reflect the comprehensive utilization of land and the overall output level,which is the most basic and commonly used indicator in assessing land use efficiency.This thesis aims to analyze the spatial and temporal changes of integrated land productivity in Chongqing from 1997 to 2023 in order to assess its land use efficiency.[Methods]This study measured the integrated land productivity of Chongqing Municipality,the only municipality directly under the central government in the western part of China,over the past 26 years(1997-2023)through relevant surveys and statistical data,and analyzed in depth the integrated land productivity of the 38 districts and counties under the jurisdiction of Chongqing,as well as the functional sub-districts of the"one district and two clusters"and the"one district and two clusters"in Chongqing.It also analyzes the characteristics of spatial and temporal differences in land productivity in 38 districts and counties under the jurisdiction of Chongqing and"one district and two clusters".[Results]The results of the study show that over the past 26 years,the integrated land productivity of Chongqing has shown an annual growth trend,and the integrated land productivity of the 38 districts and counties and the functional subregions of"one district and two clusters"has also increased significantly,but the average annual growth rate of the integrated land productivity varies among different regions.From the perspective of spatial differences,there are significant differences in land productivity among the 38 districts and counties of Chongqing and the functional subregions of"one district and two clusters",which are mainly due to the different natural conditions,economic development levels and functional positioning of each region.[Conclusions]Based on the results of the study and the actual situation of Chongqing,this paper puts forward the leading measures to improve the integrated land productivity,with a view to providing a reference basis for Chongqing to improve the efficiency of land use and promote the sustainable use of land resources.展开更多
The sustenance of humanity is contingent upon the production of food.The foundation of this production is agriculture,which in turn is dependent upon the cultivation of the land.As a fundamental element of agricultura...The sustenance of humanity is contingent upon the production of food.The foundation of this production is agriculture,which in turn is dependent upon the cultivation of the land.As a fundamental element of agricultural advancement,the rational development and utilization of land play a pivotal role in the process of rural revitalization.Agricultural land productivity serves as a principal indicator of the efficacy of land utilization and the extent of agricultural advancement.Nevertheless,there is a paucity of research examining the productivity of agricultural land,particularly a lack of specialized research on large agricultural provinces.In light of the aforementioned considerations,this paper presents a comprehensive examination of agricultural land productivity and its regional variations in Heilongjiang Province in 2022,with the aid of pertinent statistical data.The findings of the analysis indicate that among the prefecture-level cities,Daqing and Suihua exhibit the highest levels of agricultural land productivity.Additionally,Zhaodong City,Zhaozhou,Qinggang,Wangkui,and Lanxi counties exhibit high levels of agricultural land productivity within their respective prefecture-level cities.There are notable disparities in agricultural land productivity across various regions,including Yichun,Heihe,Harbin,Daqing,Hegang,and Suihua.In contrast,other regions demonstrate a more balanced spatial distribution.In order to facilitate the prosperous development of the agricultural industry in Heilongjiang Province,it is essential to optimize the spatial planning of the land,to investigate the potential for agricultural development in each region,to establish effective collaboration between resources and industries,and to create a development synergy that will collectively advance rural revitalization.展开更多
With the deepening of reform and opening up,the rural land system has experienced the evolution from contracting to transfer to the current rural revitalization strategy,and has always been closely linked to the well-...With the deepening of reform and opening up,the rural land system has experienced the evolution from contracting to transfer to the current rural revitalization strategy,and has always been closely linked to the well-being of farmers and the vigorous development of the rural economy.The reform of this system is not only a key driving force for rural economic development,but also an important cornerstone for realizing the rural revitalization strategy.Through in-depth analysis of the connotation of rural revitalization and rural land system reform,this study discusses in detail the internal dynamic mechanism of rural land system reform driving rural revitalization,and comprehensively analyzes the current implementation of rural land system reform.On this basis,this study puts forward the strategic concept of promoting the rural land system reform in the context of rural revitalization.Studies have shown that the rural land system reform has mainly played a positive role in the following four aspects in promoting rural revitalization:providing a solid material foundation for rural revitalization,optimizing the allocation of rural resources,promoting the transformation and upgrading of rural industrial structure,and promoting rural social harmony and stability.In the context of the current rural revitalization,the rural land system reform is undoubtedly one of the core issues of China s rural development.In order to further improve this system,we need to continue to optimize and innovate the rural land transfer system,the homestead reform system,and the rural collective construction land market entry system.展开更多
[Objectives]To explore the relationship between rural industry revitalization and land resource utilization.[Methods]Starting from the connotation of rural industry revitalization and land resource utilization,this st...[Objectives]To explore the relationship between rural industry revitalization and land resource utilization.[Methods]Starting from the connotation of rural industry revitalization and land resource utilization,this study explores the relationship between rural industry revitalization and land resource utilization,Taking Ganning Town with typical mountain industry as the research area,this paper analyzed the relationship between rural industry revitalization and land resource utilization through a combination of field research and literature analysis,found out the existing problems and came up with pertinent recommendations.[Results](i)Rural industry revitalization is both mutually reinforcing and mutually restrictive.(ii)In this study,the land use types of Ganning Town were divided into nine categories,including farmland,garden land,forest land and grassland,and their industrial functions and development models were identified,and the corresponding framework of land resource utilization and rural industry revitalization in Ganning Town was constructed.(iii)In view of the problems existing in Ganning Town,this paper proposed to change the ideological concept and enhance understanding,improve the utilization rate of idle land and promote rural industry revitalization,and accelerate land consolidation and restoration,and promote sustainable development.[Conclusions]It is necessary to correctly understand the two-way role and dynamic change relationship between rural industry revitalization and land resource utilization,solve the problem from the perspective of development,adopt development strategies according to local conditions,and take the road of sustainable development.展开更多
Deep understanding of the fluorescence quenching mechanisms of probes plays a crucial role in developing their practical applications.The fluorescence quenching mechanism of hydrazine-based fluorescence probes needs t...Deep understanding of the fluorescence quenching mechanisms of probes plays a crucial role in developing their practical applications.The fluorescence quenching mechanism of hydrazine-based fluorescence probes needs to be clarified up to the present.Herein,we designed and synthesized a new hydrazine-based fluorescence probe(HA-Na)based on the naphthalimide skeleton.We clarified the molecular origin of the non-fluorescence of this probe with the aid of computational chemistry and spectroscopic analysis.We showed that the significant rotation of the hydrazine group in the excited state potential energy surface,which caused the complete charge separation,was responsible for the fluorescence quenching of the probe in an organic solvent.Once the rotation was prevented in an aggregative state or high-viscosity solution,the fluorescence of the probe recovered.In other words,the fluorescence quenching mechanism of hydrazine-based fluorescence probes was attributed to the formation of a twisted intramolecular charge transfer(TICT)state.More importantly,we demonstrated that this fluorescence molecular rotor could be used to monitor the autophagy process in living cells by detecting lysosomal viscosity changes during starvation.Altogether,this work provides an essential theoretical basis for the developing potential hydrazine-based fluorescence molecular rotors.展开更多
Hematologic malignancies are one of the most common cancers,and the incidence has been rising in recent decades.The clinical and molecular features of hematologic malignancies are highly heterogenous,and some hematolo...Hematologic malignancies are one of the most common cancers,and the incidence has been rising in recent decades.The clinical and molecular features of hematologic malignancies are highly heterogenous,and some hematologic malignancies are incurable,challenging the treatment,and prognosis of the patients.However,hematopoiesis and oncogenesis of hematologic malignancies are profoundly affected by epigenetic regulation.Studies have found that methylation-related mutations,abnormal methylation profiles of DNA,and abnormal histone deacetylase expression are recurrent in leukemia and lymphoma.Furthermore,the hypomethylating agents and histone deacetylase inhibitors are effective to treat acute myeloid leukemia and T-cell lymphomas,indicating that epigenetic regulation is indispensable to hematologic oncogenesis.Epigenetic regulation mainly includes DNA modifications,histone modifications,and noncoding RNA-mediated targeting,and regulates various DNA-based processes.This review presents the role of writers,readers,and erasers of DNA methylation and histone methylation,and acetylation in hematologic malignancies.In addition,this review provides the influence of microRNAs and long noncoding RNAs on hematologic malignancies.Furthermore,the implication of epigenetic regulation in targeted treatment is discussed.This review comprehensively presents the change and function of each epigenetic regulator in normal and oncogenic hematopoiesis and provides innovative epigenetic-targeted treatment in clinical practice.展开更多
基金supported by Achievement Transformation Project(No.CGZH21001)1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(No.ZYJC21007)+4 种基金Translational Research Grant of NCRCH(No.2021WWB03)Chengdu Science and Technology Program(No.2022-YF05-01444-SN)Key Research and Development Program of Sichuan Province(No.2023YFS0031)National Key Research and Development Program of China(Nos.2022YFC2502600,2022YFC2502603)National Natural Science Foundation of China(No.82370192).
文摘Background:Dihydrolipoamide S-acetyltransferase(DLAT)is a subunit of the pyruvate dehydrogenase complex(PDC),a rate-limiting enzyme complex,that can participate in either glycolysis or the tricarboxylic acid cycle(TCA).However,the pathogenesis is not fully understood.We aimed to perform a more systematic and comprehensive analysis of DLAT in the occurrence and progression of tumors,and to investigate its function in patients’prognosis and immunotherapy.Methods:The differential expression,diagnosis,prognosis,genetic and epigenetic alterations,tumor microenvironment,stemness,immune infiltration cells,function enrichment,single-cell analysis,and drug response across cancers were conducted based on multiple computational tools.Additionally,we validated its carcinogenic effect and possible mechanism in glioma cells.Results:We exhibited that DLAT expression was increased in most tumors,especially in glioma,and affected the survival of tumor patients.DLAT was related to RNA modification genes,DNA methylation,immune infiltration,and immune infiltration cells,including CD4+T cells,CD8+T cells,Tregs,and cancer-associatedfibroblasts.Single-cell analysis displayed that DLAT might regulate cancer by mediating angiogenesis,inflammation,and stemness.Enrichment analysis revealed that DLAT might take part in the cell cycle pathway.Increased expression of DLAT leads tumor cells to be more resistant to many kinds of compounds,including PI3Kβinhibitors,PKC inhibitors,HSP90 inhibitors,and MEK inhibitors.In addition,glioma cells with DLAT silence inhibited proliferation,migration,and invasion ability,and promoted cell apoptosis.Conclusion:We conducted a comprehensive analysis of DLAT in the occurrence and progression of tumors,and its possible functions and mechanisms.DLAT is a potential diagnostic,prognostic,and immunotherapeutic biomarker for cancer patients.
文摘[Objectives]Integrated land productivity can reflect the comprehensive utilization of land and the overall output level,which is the most basic and commonly used indicator in assessing land use efficiency.This thesis aims to analyze the spatial and temporal changes of integrated land productivity in Chongqing from 1997 to 2023 in order to assess its land use efficiency.[Methods]This study measured the integrated land productivity of Chongqing Municipality,the only municipality directly under the central government in the western part of China,over the past 26 years(1997-2023)through relevant surveys and statistical data,and analyzed in depth the integrated land productivity of the 38 districts and counties under the jurisdiction of Chongqing,as well as the functional sub-districts of the"one district and two clusters"and the"one district and two clusters"in Chongqing.It also analyzes the characteristics of spatial and temporal differences in land productivity in 38 districts and counties under the jurisdiction of Chongqing and"one district and two clusters".[Results]The results of the study show that over the past 26 years,the integrated land productivity of Chongqing has shown an annual growth trend,and the integrated land productivity of the 38 districts and counties and the functional subregions of"one district and two clusters"has also increased significantly,but the average annual growth rate of the integrated land productivity varies among different regions.From the perspective of spatial differences,there are significant differences in land productivity among the 38 districts and counties of Chongqing and the functional subregions of"one district and two clusters",which are mainly due to the different natural conditions,economic development levels and functional positioning of each region.[Conclusions]Based on the results of the study and the actual situation of Chongqing,this paper puts forward the leading measures to improve the integrated land productivity,with a view to providing a reference basis for Chongqing to improve the efficiency of land use and promote the sustainable use of land resources.
文摘The sustenance of humanity is contingent upon the production of food.The foundation of this production is agriculture,which in turn is dependent upon the cultivation of the land.As a fundamental element of agricultural advancement,the rational development and utilization of land play a pivotal role in the process of rural revitalization.Agricultural land productivity serves as a principal indicator of the efficacy of land utilization and the extent of agricultural advancement.Nevertheless,there is a paucity of research examining the productivity of agricultural land,particularly a lack of specialized research on large agricultural provinces.In light of the aforementioned considerations,this paper presents a comprehensive examination of agricultural land productivity and its regional variations in Heilongjiang Province in 2022,with the aid of pertinent statistical data.The findings of the analysis indicate that among the prefecture-level cities,Daqing and Suihua exhibit the highest levels of agricultural land productivity.Additionally,Zhaodong City,Zhaozhou,Qinggang,Wangkui,and Lanxi counties exhibit high levels of agricultural land productivity within their respective prefecture-level cities.There are notable disparities in agricultural land productivity across various regions,including Yichun,Heihe,Harbin,Daqing,Hegang,and Suihua.In contrast,other regions demonstrate a more balanced spatial distribution.In order to facilitate the prosperous development of the agricultural industry in Heilongjiang Province,it is essential to optimize the spatial planning of the land,to investigate the potential for agricultural development in each region,to establish effective collaboration between resources and industries,and to create a development synergy that will collectively advance rural revitalization.
文摘With the deepening of reform and opening up,the rural land system has experienced the evolution from contracting to transfer to the current rural revitalization strategy,and has always been closely linked to the well-being of farmers and the vigorous development of the rural economy.The reform of this system is not only a key driving force for rural economic development,but also an important cornerstone for realizing the rural revitalization strategy.Through in-depth analysis of the connotation of rural revitalization and rural land system reform,this study discusses in detail the internal dynamic mechanism of rural land system reform driving rural revitalization,and comprehensively analyzes the current implementation of rural land system reform.On this basis,this study puts forward the strategic concept of promoting the rural land system reform in the context of rural revitalization.Studies have shown that the rural land system reform has mainly played a positive role in the following four aspects in promoting rural revitalization:providing a solid material foundation for rural revitalization,optimizing the allocation of rural resources,promoting the transformation and upgrading of rural industrial structure,and promoting rural social harmony and stability.In the context of the current rural revitalization,the rural land system reform is undoubtedly one of the core issues of China s rural development.In order to further improve this system,we need to continue to optimize and innovate the rural land transfer system,the homestead reform system,and the rural collective construction land market entry system.
基金Supported by National Natural Science Foundation of China(41261018).
文摘[Objectives]To explore the relationship between rural industry revitalization and land resource utilization.[Methods]Starting from the connotation of rural industry revitalization and land resource utilization,this study explores the relationship between rural industry revitalization and land resource utilization,Taking Ganning Town with typical mountain industry as the research area,this paper analyzed the relationship between rural industry revitalization and land resource utilization through a combination of field research and literature analysis,found out the existing problems and came up with pertinent recommendations.[Results](i)Rural industry revitalization is both mutually reinforcing and mutually restrictive.(ii)In this study,the land use types of Ganning Town were divided into nine categories,including farmland,garden land,forest land and grassland,and their industrial functions and development models were identified,and the corresponding framework of land resource utilization and rural industry revitalization in Ganning Town was constructed.(iii)In view of the problems existing in Ganning Town,this paper proposed to change the ideological concept and enhance understanding,improve the utilization rate of idle land and promote rural industry revitalization,and accelerate land consolidation and restoration,and promote sustainable development.[Conclusions]It is necessary to correctly understand the two-way role and dynamic change relationship between rural industry revitalization and land resource utilization,solve the problem from the perspective of development,adopt development strategies according to local conditions,and take the road of sustainable development.
基金supported by the Hainan Provincial Natural Science Foundation of China(123MS001 and 123QN181)the National Natural Science Foundation of China(32360236,52063011,22203024)+2 种基金the Collaborative Innovation Center Foundation of the Hainan University(XTCX2022JKB03)the Research Start-up Fund Project of Hainan University(No.RZ2200001217 and RZ2200001216)Tianjin University-Hainan University Independent Innovation Fund(RZ2200003795).
文摘Deep understanding of the fluorescence quenching mechanisms of probes plays a crucial role in developing their practical applications.The fluorescence quenching mechanism of hydrazine-based fluorescence probes needs to be clarified up to the present.Herein,we designed and synthesized a new hydrazine-based fluorescence probe(HA-Na)based on the naphthalimide skeleton.We clarified the molecular origin of the non-fluorescence of this probe with the aid of computational chemistry and spectroscopic analysis.We showed that the significant rotation of the hydrazine group in the excited state potential energy surface,which caused the complete charge separation,was responsible for the fluorescence quenching of the probe in an organic solvent.Once the rotation was prevented in an aggregative state or high-viscosity solution,the fluorescence of the probe recovered.In other words,the fluorescence quenching mechanism of hydrazine-based fluorescence probes was attributed to the formation of a twisted intramolecular charge transfer(TICT)state.More importantly,we demonstrated that this fluorescence molecular rotor could be used to monitor the autophagy process in living cells by detecting lysosomal viscosity changes during starvation.Altogether,this work provides an essential theoretical basis for the developing potential hydrazine-based fluorescence molecular rotors.
基金This work is supported by the National Natural Science Foundation of China(82204490 to A.Z.)the Translational Research Grant of NCRCH(2021WWB03 to T.N.)+1 种基金Achievement Transformation Project(CGZH21001 to T.N.)1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(ZYJC21007 to T.N.).
文摘Hematologic malignancies are one of the most common cancers,and the incidence has been rising in recent decades.The clinical and molecular features of hematologic malignancies are highly heterogenous,and some hematologic malignancies are incurable,challenging the treatment,and prognosis of the patients.However,hematopoiesis and oncogenesis of hematologic malignancies are profoundly affected by epigenetic regulation.Studies have found that methylation-related mutations,abnormal methylation profiles of DNA,and abnormal histone deacetylase expression are recurrent in leukemia and lymphoma.Furthermore,the hypomethylating agents and histone deacetylase inhibitors are effective to treat acute myeloid leukemia and T-cell lymphomas,indicating that epigenetic regulation is indispensable to hematologic oncogenesis.Epigenetic regulation mainly includes DNA modifications,histone modifications,and noncoding RNA-mediated targeting,and regulates various DNA-based processes.This review presents the role of writers,readers,and erasers of DNA methylation and histone methylation,and acetylation in hematologic malignancies.In addition,this review provides the influence of microRNAs and long noncoding RNAs on hematologic malignancies.Furthermore,the implication of epigenetic regulation in targeted treatment is discussed.This review comprehensively presents the change and function of each epigenetic regulator in normal and oncogenic hematopoiesis and provides innovative epigenetic-targeted treatment in clinical practice.
