Objective: To develop a best-evidence-based optimal nutrition management plan for patients with chronic heart failure, apply it in clinical practice, and evaluate its effectiveness. Methods: Use the KTA knowledge tran...Objective: To develop a best-evidence-based optimal nutrition management plan for patients with chronic heart failure, apply it in clinical practice, and evaluate its effectiveness. Methods: Use the KTA knowledge translation model to guide evidence-based practice in nutrition management, and compare the nutritional status, cardiac function status, quality of life, and quality review indicators of chronic heart failure patients before and after the application of evidence. Results: After the application of evidence, the nutritional status indicators (MNA-SF score, albumin, hemoglobin) of two groups of heart failure patients significantly increased compared to before the application of evidence, with statistically significant differences (p Conclusion: The KTA knowledge translation model provides methodological guidance for the implementation of evidence-based practice for heart failure patients. This evidence-based practice project is beneficial for improving the outcomes of malnutrition in chronic heart failure patients and is conducive to standardizing nursing pathways, thereby promoting the improvement of nursing quality.展开更多
Objective:To investigate the effects and mechanisms of Xuebijing injection(XBJ)on Chimeric antigen receptor-T(CAR-T)cell function and its therapeutic potential against CAR-T therapy-associated cytokine storms(CRS).Met...Objective:To investigate the effects and mechanisms of Xuebijing injection(XBJ)on Chimeric antigen receptor-T(CAR-T)cell function and its therapeutic potential against CAR-T therapy-associated cytokine storms(CRS).Methods:Anti-CD19 CAR-T cells were established based on FMC63 antibodies.Different doses of XBJ(1 and 10 mg/mL)were added to the culture system.Untreated anti-CD19 CAR-T cells served as negative controls.After 48-h co-culture,the effects of XBJ on CAR-T cell function were assessed.Carboxyfluorescein diacetate succinimidyl ester staining was used to assess the effect of XBJ on CAR-T cell proliferation.Flow cytometry,luciferase reporter gene assays,and real time cellular analysis were employed to evaluate the effects of XBJ on CAR-T cell cytotoxicity in vitro.RNA-sequencing was performed to analyze the effects of XBJ on CAR-T cell gene expression.Network pharmacology predicted potential XBJ therapeutic targets for CRS,which were verified in a THP-1 macrophage inflammation model.Results:XBJ enhanced both the proliferation and tumor killing capacities of CAR-T cells.Transcriptome analysis showed that XBJ treatment affects multiple genes and pathways in CAR-T cells,with differential gene enrichment in multiple cell proliferation and growth factor pathways.Potential targets for CRS control by XBJ were predicted using network pharmacology,and the inhibitory effect of XBJ on the expression of relevant genes was verified using a macrophage model.Conclusion:The results of this study indicate that XBJ can enhance the killing effect of CAR-T cells on tumor cells and that the mechanism is related to the regulation of T cell proliferation and activation.Moreover,XBJ inhibited excessive inflammation associated with CAR-T therapy.However,the current findings remain to be further validated through in vivo experiments.展开更多
Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and memory.Kinesin-4 KIF21A helps organize the microtubule-actin network at th...Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and memory.Kinesin-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains unknown.In this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative spines.Furthermore,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic plasticity.Knockdown of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and talin1.Knocking down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive abilities.Taken together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.展开更多
Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational levels.tsRNAs are not on...Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational levels.tsRNAs are not only degradation fragments but also have biological functions,including those in immune inflammation,metabolic disorders,and cell death.tsRNA dysregulation is closely associated with multiple diseases,including various cancers and acute pancreatitis(AP).AP is a common gastrointestinal disease,and its incidence increases annually.AP development is associated with tsRNAs,which regulate cell injury and induce inflammation,especially pyroptosis and ferroptosis.Notably,serum tRF36 has the potential to serve as a non-invasive diagnostic biomarker and leads to pancreatic acinar cell ferroptosis causing inflammation to promote AP.We show the characteristics of tsRNAs and their diagnostic value and function in AP,and discuss the potential opportunities and challenges of using tsRNAs in clinical applications and research.展开更多
BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and te...BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and teenagers, but the maintenance of pulphealth and the calcific healing of multiple root fractures are rarely reported in theliterature.CASE SUMMARY This case reports healing of a permanent tooth with complicated crown–root andadditional root fractures, in which pulp health was maintained. A 10-year-old girlfell and fractured the root of her maxillary left central incisor at the cervical level.After the coronal fragment was repositioned, the tooth was splinted until thetooth was no longer mobile, 2 years later. Eight years after treatment, the toothhas remained asymptomatic with vital pulp and localized gingival overgrowth.Cone-beam computed tomography revealed not only calcified healing of the CRFbut also spontaneous healing in an additional undiagnosed root fracture. Thefracture line on the enamel could not be healed by hard tissue and formed agroove in the cervical crown. It was speculated that the groove was related to thelocalized gingival overgrowth.CONCLUSION This case provides a clinical perspective of the treatment of a tooth with acomplicated CRF and an additional root fracture.展开更多
We are deeply interested in the recent findings onβ-arrestin 2.Liu et al demonstrated thatβ-arrestin 2 knockout provides significant protection in diabetic nephropathy,underscoring its potential as a promising thera...We are deeply interested in the recent findings onβ-arrestin 2.Liu et al demonstrated thatβ-arrestin 2 knockout provides significant protection in diabetic nephropathy,underscoring its potential as a promising therapeutic target for diabetic nephropathy treatment.Furthermore,the role ofβ-arrestin 2 in metabolic regulation is equally critical,particularly in insulin signaling,hepatic glucose production,and adipose tissue function.Althoughβ-arrestin 2 plays a distinct role in metabolism and kidney protection,its tissue-specific regulation opens up valuable avenues for developing targeted therapeutic strategies centered onβ-arrestin 2.展开更多
This work is focused on a quasi-Euclidean space with UV cutoff, IR cutoff and symmetries. Mathematical analysis reveals that the UV cutoff results in the minimum structures of space. Dominated by rotational symmetry, ...This work is focused on a quasi-Euclidean space with UV cutoff, IR cutoff and symmetries. Mathematical analysis reveals that the UV cutoff results in the minimum structures of space. Dominated by rotational symmetry, the structure should be a local one in situ or on a sphere. Investigations show that a 10D minimum structure is a non-local one with transformability between in-situ state and spherical state due to its special topology. Based on the quantum behaviors of the 10D structure controlled by translational symmetry, IR cutoff determines two long-range interactions with dimensionless constants of ~1/137.036 and ~1/1.628E+38, respectively.展开更多
Lithium argyrodites with high ionic conductivity and low cost are considered as one of the most prospective solid electrolytes for all-solid-state lithium batteries.However,the poor chemical stability and compatibilit...Lithium argyrodites with high ionic conductivity and low cost are considered as one of the most prospective solid electrolytes for all-solid-state lithium batteries.However,the poor chemical stability and compatibility with lithium metal limit their application.Herein,Li_(5.4)PS_(4.4)Cl1.4I0.2solid electrolyte with high ionic conductivity of 11.49 m S ccm^(-1)and improved chemical stability is synthesized by iodine doping.An ultra-thin Li_(5.4)PS_(4.4)Cl_(1.4)I_(0.2)membrane with thickness of 10μm can be obtained by wet coating process,exhibiting a high ionic conductivity of 2.09 mS ccm^(-1)and low areal resistance of 1.17Ωcm^(-2).Moreover,iodine doping could in-situ form LiI at the lithium/electrolyte interface and improve the critical current density of Li_(5.4)PS_(4.4)Cl_(1.6)from 0.8 to 1.35 mA cm^(-2).The resultant LiCoO_(2)/Li_(5.4)PS_(4.4)Cl_(1.4)I_(0.2)/Li battery shows excellent cycling stability at 1 C,with a reversible specific capacity of 110.1 mA h g^(-1)and a retention of 80.5% after 1000 cycles.In addition,the assembled LiCoO_(2)/Li_(5.4)PS_(4.4)Cl_(1.4)I_(0.2)membrane/Li pouch cell delivers an initial discharge capacity of 110.4 mA h g^(-1)and 80.5% capacity retention after 100 cycles.展开更多
BACKGROUND Most non-small cell lung cancer patients have epidermal growth factor receptor(EGFR)activating mutations,such as exon 19 deletion and exon 21 replacement mutations.Osimertinib is a third-generation EGFR-tyr...BACKGROUND Most non-small cell lung cancer patients have epidermal growth factor receptor(EGFR)activating mutations,such as exon 19 deletion and exon 21 replacement mutations.Osimertinib is a third-generation EGFR-tyrosine kinase inhibitors ap-proved for the treatment of lung cancer patients carrying EGFR activating mu-tations.Osimertinib-induced interstitial lung disease(ILD)is a rare and poten-tially fatal pulmonary toxic manifestation of drug therapy.At present,there is no international consensus on the risks and treatment of the osimertinib-induced ILD.CASE SUMMARY We report a case of a 56-year-old woman who was diagnosed with lung adenocar-cinoma with lung hilum,mediastinal lymph nodes and brain metastases(T4N3-M1c stage IVB).The patient received targeted treatment with osimertinib after radiotherapy and chemotherapy.But she developed ILD after osimertinib treat-ment.Following active symptomatic treatment and hormone treatment,the lung injury alleviated.The patient was retreated with furmonertinib combined with prednisone and did not experience ILD again.So far,she has survived for 14 months without disease progression.CONCLUSION Retreatment with furmonertinib under prednisone could be considered as an effective therapeutic option after risk-benefit assessment for EGFR-mutant lung adenocarcinoma patients.展开更多
In this study,an end-to-end deep learning method is proposed to improve the accuracy of continuum estimation in low-resolution gamma-ray spectra.A novel process for generating the theoretical continuum of a simulated ...In this study,an end-to-end deep learning method is proposed to improve the accuracy of continuum estimation in low-resolution gamma-ray spectra.A novel process for generating the theoretical continuum of a simulated spectrum is established,and a convolutional neural network consisting of 51 layers and more than 105 parameters is constructed to directly predict the entire continuum from the extracted global spectrum features.For testing,an in-house NaI-type whole-body counter is used,and 106 training spectrum samples(20%of which are reserved for testing)are generated using Monte Carlo simulations.In addition,the existing fitting,step-type,and peak erosion methods are selected for comparison.The proposed method exhibits excellent performance,as evidenced by its activity error distribution and the smallest mean activity error of 1.5%among the evaluated methods.Additionally,a validation experiment is performed using a whole-body counter to analyze a human physical phantom containing four radionuclides.The largest activity error of the proposed method is−5.1%,which is considerably smaller than those of the comparative methods,confirming the test results.The multiscale feature extraction and nonlinear relation modeling in the proposed method establish a novel approach for accurate and convenient continuum estimation in a low-resolution gamma-ray spectrum.Thus,the proposed method is promising for accurate quantitative radioactivity analysis in practical applications.展开更多
Rechargeable aqueous zinc-metal batteries (AZMBs) are promising candidates for large-scale energy storage systems due to their low cost and high safety.However,their performance and sustainability are significantly hi...Rechargeable aqueous zinc-metal batteries (AZMBs) are promising candidates for large-scale energy storage systems due to their low cost and high safety.However,their performance and sustainability are significantly hindered by the sluggish desolvation kinetics at the electrode/electrolyte interface and the corresponding hydrogen evolution reaction where active water molecules tightly participate in the Zn(H_(2)O)_(6)^(2+)solvation shell.Herein,learnt from self-generated solid electrolyte interphase (SEI) in anodes,the dielectric but ion-conductive zinc niobate nanoparticles artificial layer is constructed on metallic Zn surface (ZNB@Zn),acting as a rapid desolvation promotor.The zincophilic and dielectric-conductive properties of ZNB layer accelerate interfacial desolvation/diffusion and suppress surface corrosion or dendrite formation,achieving uniform Zn plating/stripping behavior,as confirmed by electronic/optical microscopies and interface spectroscopical measurements together with theoretical calculations.Consequently,the as-prepared ZNB@Zn electrode exhibits excellent cycling stability of over 2000 h and robust reversibility (99.54%) even under high current density and depth of discharge conditions.Meanwhile,the assembled ZNB@Zn-based full cell displays high capacity-retention rate of 80.21%after 3000 cycles at 5 A g^(-1)and outstanding rate performance up to 10 A g^(-1).The large-areal pouch cell is stabilized for hundreds of cycles,highlighting the bright prospects of the dielectric but ion-conductive layer in further application of AZMBs.展开更多
High-entropy materials have become high-activity electrocatalysis owing to their high-entropy effect and multiple active sites.Herein,we synthesize a series of carbon-supported nano high-entropy oxides(HEOs/C),specifi...High-entropy materials have become high-activity electrocatalysis owing to their high-entropy effect and multiple active sites.Herein,we synthesize a series of carbon-supported nano high-entropy oxides(HEOs/C),specifically (PtFeCoNiCu)O/C,using a carbothermal shock (CTS) method for application as a cathode catalyst in direct borohydride fuel cells (DBFCs).The microstructure of the prepared catalysts was characterized by X-ray photoelectron spectroscopy,X-ray absorption fine structure,and transmission electron microscopy.The prepared (PtFeCoNiCu)O/C,with particle sizes ranging from 2 to 4 nm,demonstrates 3.94 transferred electrons towards the oxygen reduction reaction in an alkaline environment,resulting in a minimal H_(2)O_(2)yield of 2.6%.Additionally,it exhibits a Tafel slope of 61 mV dec-1,surpassing that of commercial Pt/C (82 mV dec-1).Furthermore,after 40,000 cycles of cyclic voltammetry(CV) testing,the half-wave potential of (PtFeCoNiCu)O/C shows a positive shift of 3 mV,with no notable decline in the limiting current density.When (PtFeCoNiCu)O/C is used as a cathode catalyst in DBFCs,the DBFC achieves a maximum power density of 441 mW cm^(-2)at 60°C and sustains a cell voltage of approximately 0.73 V after 52 h at 30°C.These findings confirm that HEO/C is a promising cathode catalyst for DBFCs.展开更多
Objective:To observe the effect of Xuebijing,a complex traditional Chinese preparation,on inflammation and prognosis of patients with pneumonia.Methods:The patients with ventilator-associated pneumonia in the intensiv...Objective:To observe the effect of Xuebijing,a complex traditional Chinese preparation,on inflammation and prognosis of patients with pneumonia.Methods:The patients with ventilator-associated pneumonia in the intensive care unit(ICU)were randomly divided into the control group and the treatment group with 35 cases in each group.Both groups were given routine treatment such as anti-inflammatory drugs,rehydration,expectorant,and nutritional support,while the treatment group was additionally given Xuebijing injection.Serum C-reactive protein(CRP),clinical pulmonary infection score(CPIS),acute physiology,and chronic health scoreⅡ(APACHEⅡ)were recorded before treatment,the 3rd and 7th day after treatment.The duration of antibiotic use,mechanical ventilation,ICU stay,and mortality during 28 days was recorded.Results:There was no significant difference in CRP,CPIS,and APACHEⅡbetween the two groups before treatment(P>0.05).The improvement of CRP,CPIS,and APACHEⅡin the treatment group was better than those in the control group on the 3 and 7 days after treatment,and the differences were statistically significant(P<0.05).The duration of antibiotic use,mechanical ventilation,and ICU stay in the treatment group were less than those in the control group(P<0.05).The 28-day mortality of the treatment group was lower than that of the control group,but the difference was not statistically significant(P>0.05).Conclusions:Xuebijing injection can improve the inflammatory indexes of patients with ventilator-associated pneumonia,and can partly improve the prognosis.展开更多
Objective:The objective of this study is to establish a nursing standard of integrated traditional Chinese and Western medicine for patients with COVID‑19(mild and common)in Beijing,to provide reference for clinical n...Objective:The objective of this study is to establish a nursing standard of integrated traditional Chinese and Western medicine for patients with COVID‑19(mild and common)in Beijing,to provide reference for clinical nursing of patients with COVID‑19(mild and common).Methods:Through online communication meeting with nurses who are in the frontline of anti‑epidemic,clinical investigation,literature research,and expert demonstration meeting are carried out to prepare the draft of the standard,and the Delphi method is applied to determine the standard of integrated traditional Chinese and Western medicine care for patients with COVID‑19(mild and common)in Beijing.Results:The nursing standard of integrated traditional Chinese and Western medicine for patients with COVID‑19(mild and common)was established,which included 5 first‑level indicators,14 second-level indicators and 60 third‑level indicators.After two rounds of Delphi method,the positive coefficients of experts were 96%and 83%,the authoritative coefficients of experts were 0.89 and 0.91,and the Kendall’s coefficient of concordance(W)of experts were 0.12,0.09,0.10,0.13(P<0.05)and 0.44,0.43,0.37,0.39(P<0.05).Conclusion:The standard of integrated traditional Chinese and Western medicine nursing for patients with COVID‑19(mild and common)in Beijing constructed by the Delphi method is scientific and practical,which provides a reference for clinical application of integrated traditional Chinese and Western medicine nursing to fight against COVID‑19 infection.展开更多
Birds exhibit extraordinary mobility and remarkable navigational skills,obtaining guidance cues from the Earth’s magnetic field for orientation and long-distance movement.Bird species also show tremendous diversity i...Birds exhibit extraordinary mobility and remarkable navigational skills,obtaining guidance cues from the Earth’s magnetic field for orientation and long-distance movement.Bird species also show tremendous diversity in navigation strategies,with considerable differences even within the same taxa and among individuals from the same population.The highly conserved iron and iron-sulfur cluster binding magnetoreceptor(MagR)protein is suggested to enable animals,including birds,to detect the geomagnetic field and navigate accordingly.Notably,MagR is also implicated in other functions,such as electron transfer and biogenesis of iron-sulfur clusters,raising the question of whether variability exists in its biochemical and biophysical features among species,particularly birds.In the current study,we conducted a comparative analysis of MagR from two different bird species,including the migratory European robin(Erithacus rubecula)and the homing pigeon(Columba livia).Sequence alignment revealed an extremely high degree of similarity between the MagRs of these species,with only three sequence variations.Nevertheless,two of these variations underpinned significant differences in metal binding capacity,oligomeric state,and magnetic properties.These findings offer compelling evidence for the marked differences in MagR between the two avian species,potentially explaining how a highly conserved protein can mediate such diverse functions.展开更多
文摘Objective: To develop a best-evidence-based optimal nutrition management plan for patients with chronic heart failure, apply it in clinical practice, and evaluate its effectiveness. Methods: Use the KTA knowledge translation model to guide evidence-based practice in nutrition management, and compare the nutritional status, cardiac function status, quality of life, and quality review indicators of chronic heart failure patients before and after the application of evidence. Results: After the application of evidence, the nutritional status indicators (MNA-SF score, albumin, hemoglobin) of two groups of heart failure patients significantly increased compared to before the application of evidence, with statistically significant differences (p Conclusion: The KTA knowledge translation model provides methodological guidance for the implementation of evidence-based practice for heart failure patients. This evidence-based practice project is beneficial for improving the outcomes of malnutrition in chronic heart failure patients and is conducive to standardizing nursing pathways, thereby promoting the improvement of nursing quality.
基金supported by the Beijing Double Class One Human Resource Office-High-Level-Jianxun Wang's Research Team Funding Project of China(1000041510155)the Research Startup Fund Program at the Beijing University of Chinese Medicine(90011451310032).
文摘Objective:To investigate the effects and mechanisms of Xuebijing injection(XBJ)on Chimeric antigen receptor-T(CAR-T)cell function and its therapeutic potential against CAR-T therapy-associated cytokine storms(CRS).Methods:Anti-CD19 CAR-T cells were established based on FMC63 antibodies.Different doses of XBJ(1 and 10 mg/mL)were added to the culture system.Untreated anti-CD19 CAR-T cells served as negative controls.After 48-h co-culture,the effects of XBJ on CAR-T cell function were assessed.Carboxyfluorescein diacetate succinimidyl ester staining was used to assess the effect of XBJ on CAR-T cell proliferation.Flow cytometry,luciferase reporter gene assays,and real time cellular analysis were employed to evaluate the effects of XBJ on CAR-T cell cytotoxicity in vitro.RNA-sequencing was performed to analyze the effects of XBJ on CAR-T cell gene expression.Network pharmacology predicted potential XBJ therapeutic targets for CRS,which were verified in a THP-1 macrophage inflammation model.Results:XBJ enhanced both the proliferation and tumor killing capacities of CAR-T cells.Transcriptome analysis showed that XBJ treatment affects multiple genes and pathways in CAR-T cells,with differential gene enrichment in multiple cell proliferation and growth factor pathways.Potential targets for CRS control by XBJ were predicted using network pharmacology,and the inhibitory effect of XBJ on the expression of relevant genes was verified using a macrophage model.Conclusion:The results of this study indicate that XBJ can enhance the killing effect of CAR-T cells on tumor cells and that the mechanism is related to the regulation of T cell proliferation and activation.Moreover,XBJ inhibited excessive inflammation associated with CAR-T therapy.However,the current findings remain to be further validated through in vivo experiments.
基金supported by the National Key Research and Development Program of China,No.2021ZD0202503(to AHT)the National Natural Science Foundation of China,Nos.31872759(to AHT)and 32070707(to CF)+1 种基金Shenzhen Science and Technology Program,No.RCJC20210609104333007(to ZW)Shenzhen-Hong Kong Institute of Brain Science,Shenzhen Fundamental Research Institutions,No.2021SHIBS0002(to ZW).
文摘Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and memory.Kinesin-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains unknown.In this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative spines.Furthermore,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic plasticity.Knockdown of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and talin1.Knocking down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive abilities.Taken together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.
基金Supported by the Central South University Innovation-Driven Research Programme,No.2023CXQD075。
文摘Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational levels.tsRNAs are not only degradation fragments but also have biological functions,including those in immune inflammation,metabolic disorders,and cell death.tsRNA dysregulation is closely associated with multiple diseases,including various cancers and acute pancreatitis(AP).AP is a common gastrointestinal disease,and its incidence increases annually.AP development is associated with tsRNAs,which regulate cell injury and induce inflammation,especially pyroptosis and ferroptosis.Notably,serum tRF36 has the potential to serve as a non-invasive diagnostic biomarker and leads to pancreatic acinar cell ferroptosis causing inflammation to promote AP.We show the characteristics of tsRNAs and their diagnostic value and function in AP,and discuss the potential opportunities and challenges of using tsRNAs in clinical applications and research.
基金Supported by 2021 Disciplinary Construction Project in School of Dentistry,Anhui Medical University,No.2021kqxkFY05.
文摘BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and teenagers, but the maintenance of pulphealth and the calcific healing of multiple root fractures are rarely reported in theliterature.CASE SUMMARY This case reports healing of a permanent tooth with complicated crown–root andadditional root fractures, in which pulp health was maintained. A 10-year-old girlfell and fractured the root of her maxillary left central incisor at the cervical level.After the coronal fragment was repositioned, the tooth was splinted until thetooth was no longer mobile, 2 years later. Eight years after treatment, the toothhas remained asymptomatic with vital pulp and localized gingival overgrowth.Cone-beam computed tomography revealed not only calcified healing of the CRFbut also spontaneous healing in an additional undiagnosed root fracture. Thefracture line on the enamel could not be healed by hard tissue and formed agroove in the cervical crown. It was speculated that the groove was related to thelocalized gingival overgrowth.CONCLUSION This case provides a clinical perspective of the treatment of a tooth with acomplicated CRF and an additional root fracture.
基金Supported by National Natural Science Foundation of China,No.82471616,No.82170418,and No.82271618Natural Science Foundation of Hubei Province,No.2022CFA015+2 种基金Central Guiding Local Science and Technology Development Project,No.2022BGE237Key Research and Development Program of Hubei Province,No.2022BCE001,and No.2023BCB139Hubei Provincial Health Commission Project,No.WJ2023M151。
文摘We are deeply interested in the recent findings onβ-arrestin 2.Liu et al demonstrated thatβ-arrestin 2 knockout provides significant protection in diabetic nephropathy,underscoring its potential as a promising therapeutic target for diabetic nephropathy treatment.Furthermore,the role ofβ-arrestin 2 in metabolic regulation is equally critical,particularly in insulin signaling,hepatic glucose production,and adipose tissue function.Althoughβ-arrestin 2 plays a distinct role in metabolism and kidney protection,its tissue-specific regulation opens up valuable avenues for developing targeted therapeutic strategies centered onβ-arrestin 2.
文摘This work is focused on a quasi-Euclidean space with UV cutoff, IR cutoff and symmetries. Mathematical analysis reveals that the UV cutoff results in the minimum structures of space. Dominated by rotational symmetry, the structure should be a local one in situ or on a sphere. Investigations show that a 10D minimum structure is a non-local one with transformability between in-situ state and spherical state due to its special topology. Based on the quantum behaviors of the 10D structure controlled by translational symmetry, IR cutoff determines two long-range interactions with dimensionless constants of ~1/137.036 and ~1/1.628E+38, respectively.
基金National Key R&D Program of China (grant no. 2022YFB3807700)National Natural Science Foundation of China (Grant No. U1964205, U21A2075, 52172253,52102326, 52250610214, 22309194, 52372244)+4 种基金Ningbo S&T Innovation 2025 Major Special Programme (Grant No.2019B10044, 2021Z122, 2023Z106)Zhejiang Provincial Key R&D Program of China (Grant No. 2022C01072, 2024C01095)Jiangsu Provincial S&T Innovation Special Programme for carbon peak and carbon neutrality (Grant No. BE2022007)Baima Lake Laboratory Joint Funds of the Zhejiang Provincial Natural Science Foundation of China (LBMHD24E020001)Youth Innovation Promotion Association CAS (Y2021080)。
文摘Lithium argyrodites with high ionic conductivity and low cost are considered as one of the most prospective solid electrolytes for all-solid-state lithium batteries.However,the poor chemical stability and compatibility with lithium metal limit their application.Herein,Li_(5.4)PS_(4.4)Cl1.4I0.2solid electrolyte with high ionic conductivity of 11.49 m S ccm^(-1)and improved chemical stability is synthesized by iodine doping.An ultra-thin Li_(5.4)PS_(4.4)Cl_(1.4)I_(0.2)membrane with thickness of 10μm can be obtained by wet coating process,exhibiting a high ionic conductivity of 2.09 mS ccm^(-1)and low areal resistance of 1.17Ωcm^(-2).Moreover,iodine doping could in-situ form LiI at the lithium/electrolyte interface and improve the critical current density of Li_(5.4)PS_(4.4)Cl_(1.6)from 0.8 to 1.35 mA cm^(-2).The resultant LiCoO_(2)/Li_(5.4)PS_(4.4)Cl_(1.4)I_(0.2)/Li battery shows excellent cycling stability at 1 C,with a reversible specific capacity of 110.1 mA h g^(-1)and a retention of 80.5% after 1000 cycles.In addition,the assembled LiCoO_(2)/Li_(5.4)PS_(4.4)Cl_(1.4)I_(0.2)membrane/Li pouch cell delivers an initial discharge capacity of 110.4 mA h g^(-1)and 80.5% capacity retention after 100 cycles.
基金Supported by Guangxi Guilin Science and Technology Fund,No.20190218-7-6.
文摘BACKGROUND Most non-small cell lung cancer patients have epidermal growth factor receptor(EGFR)activating mutations,such as exon 19 deletion and exon 21 replacement mutations.Osimertinib is a third-generation EGFR-tyrosine kinase inhibitors ap-proved for the treatment of lung cancer patients carrying EGFR activating mu-tations.Osimertinib-induced interstitial lung disease(ILD)is a rare and poten-tially fatal pulmonary toxic manifestation of drug therapy.At present,there is no international consensus on the risks and treatment of the osimertinib-induced ILD.CASE SUMMARY We report a case of a 56-year-old woman who was diagnosed with lung adenocar-cinoma with lung hilum,mediastinal lymph nodes and brain metastases(T4N3-M1c stage IVB).The patient received targeted treatment with osimertinib after radiotherapy and chemotherapy.But she developed ILD after osimertinib treat-ment.Following active symptomatic treatment and hormone treatment,the lung injury alleviated.The patient was retreated with furmonertinib combined with prednisone and did not experience ILD again.So far,she has survived for 14 months without disease progression.CONCLUSION Retreatment with furmonertinib under prednisone could be considered as an effective therapeutic option after risk-benefit assessment for EGFR-mutant lung adenocarcinoma patients.
基金supported by the National Natural Science Foundation of China(No.12005198).
文摘In this study,an end-to-end deep learning method is proposed to improve the accuracy of continuum estimation in low-resolution gamma-ray spectra.A novel process for generating the theoretical continuum of a simulated spectrum is established,and a convolutional neural network consisting of 51 layers and more than 105 parameters is constructed to directly predict the entire continuum from the extracted global spectrum features.For testing,an in-house NaI-type whole-body counter is used,and 106 training spectrum samples(20%of which are reserved for testing)are generated using Monte Carlo simulations.In addition,the existing fitting,step-type,and peak erosion methods are selected for comparison.The proposed method exhibits excellent performance,as evidenced by its activity error distribution and the smallest mean activity error of 1.5%among the evaluated methods.Additionally,a validation experiment is performed using a whole-body counter to analyze a human physical phantom containing four radionuclides.The largest activity error of the proposed method is−5.1%,which is considerably smaller than those of the comparative methods,confirming the test results.The multiscale feature extraction and nonlinear relation modeling in the proposed method establish a novel approach for accurate and convenient continuum estimation in a low-resolution gamma-ray spectrum.Thus,the proposed method is promising for accurate quantitative radioactivity analysis in practical applications.
基金National Key R&D Program of China (2021YFA1201503)National Natural Science Foundation of China (21972164, 22279161, 12264038, 22309144)+4 种基金Natural Science Foundation of Jiangsu Province (BK. 20210130)China Postdoctoral Science Foundation (2023M733189)Jiangsu Double-Innovation PhD Program in 2022 (JSSCBS20221241)Senior Talents Fund of Jiangsu University (5501220014)fellowship funding provided by the Alexander von Humboldt Foundation。
文摘Rechargeable aqueous zinc-metal batteries (AZMBs) are promising candidates for large-scale energy storage systems due to their low cost and high safety.However,their performance and sustainability are significantly hindered by the sluggish desolvation kinetics at the electrode/electrolyte interface and the corresponding hydrogen evolution reaction where active water molecules tightly participate in the Zn(H_(2)O)_(6)^(2+)solvation shell.Herein,learnt from self-generated solid electrolyte interphase (SEI) in anodes,the dielectric but ion-conductive zinc niobate nanoparticles artificial layer is constructed on metallic Zn surface (ZNB@Zn),acting as a rapid desolvation promotor.The zincophilic and dielectric-conductive properties of ZNB layer accelerate interfacial desolvation/diffusion and suppress surface corrosion or dendrite formation,achieving uniform Zn plating/stripping behavior,as confirmed by electronic/optical microscopies and interface spectroscopical measurements together with theoretical calculations.Consequently,the as-prepared ZNB@Zn electrode exhibits excellent cycling stability of over 2000 h and robust reversibility (99.54%) even under high current density and depth of discharge conditions.Meanwhile,the assembled ZNB@Zn-based full cell displays high capacity-retention rate of 80.21%after 3000 cycles at 5 A g^(-1)and outstanding rate performance up to 10 A g^(-1).The large-areal pouch cell is stabilized for hundreds of cycles,highlighting the bright prospects of the dielectric but ion-conductive layer in further application of AZMBs.
基金Zhejiang Provincial Natural Science Foundation of China (LZ22B060001,LY22E010003)“Pioneer” R&D Program of Zhejiang Province(2023C01080)National Natural Science Foundation of China (52301235)。
文摘High-entropy materials have become high-activity electrocatalysis owing to their high-entropy effect and multiple active sites.Herein,we synthesize a series of carbon-supported nano high-entropy oxides(HEOs/C),specifically (PtFeCoNiCu)O/C,using a carbothermal shock (CTS) method for application as a cathode catalyst in direct borohydride fuel cells (DBFCs).The microstructure of the prepared catalysts was characterized by X-ray photoelectron spectroscopy,X-ray absorption fine structure,and transmission electron microscopy.The prepared (PtFeCoNiCu)O/C,with particle sizes ranging from 2 to 4 nm,demonstrates 3.94 transferred electrons towards the oxygen reduction reaction in an alkaline environment,resulting in a minimal H_(2)O_(2)yield of 2.6%.Additionally,it exhibits a Tafel slope of 61 mV dec-1,surpassing that of commercial Pt/C (82 mV dec-1).Furthermore,after 40,000 cycles of cyclic voltammetry(CV) testing,the half-wave potential of (PtFeCoNiCu)O/C shows a positive shift of 3 mV,with no notable decline in the limiting current density.When (PtFeCoNiCu)O/C is used as a cathode catalyst in DBFCs,the DBFC achieves a maximum power density of 441 mW cm^(-2)at 60°C and sustains a cell voltage of approximately 0.73 V after 52 h at 30°C.These findings confirm that HEO/C is a promising cathode catalyst for DBFCs.
基金This work was supported by the Talent Funding Project of Tangshan City in Hebei Province(No.a201902005).
文摘Objective:To observe the effect of Xuebijing,a complex traditional Chinese preparation,on inflammation and prognosis of patients with pneumonia.Methods:The patients with ventilator-associated pneumonia in the intensive care unit(ICU)were randomly divided into the control group and the treatment group with 35 cases in each group.Both groups were given routine treatment such as anti-inflammatory drugs,rehydration,expectorant,and nutritional support,while the treatment group was additionally given Xuebijing injection.Serum C-reactive protein(CRP),clinical pulmonary infection score(CPIS),acute physiology,and chronic health scoreⅡ(APACHEⅡ)were recorded before treatment,the 3rd and 7th day after treatment.The duration of antibiotic use,mechanical ventilation,ICU stay,and mortality during 28 days was recorded.Results:There was no significant difference in CRP,CPIS,and APACHEⅡbetween the two groups before treatment(P>0.05).The improvement of CRP,CPIS,and APACHEⅡin the treatment group was better than those in the control group on the 3 and 7 days after treatment,and the differences were statistically significant(P<0.05).The duration of antibiotic use,mechanical ventilation,and ICU stay in the treatment group were less than those in the control group(P<0.05).The 28-day mortality of the treatment group was lower than that of the control group,but the difference was not statistically significant(P>0.05).Conclusions:Xuebijing injection can improve the inflammatory indexes of patients with ventilator-associated pneumonia,and can partly improve the prognosis.
文摘Objective:The objective of this study is to establish a nursing standard of integrated traditional Chinese and Western medicine for patients with COVID‑19(mild and common)in Beijing,to provide reference for clinical nursing of patients with COVID‑19(mild and common).Methods:Through online communication meeting with nurses who are in the frontline of anti‑epidemic,clinical investigation,literature research,and expert demonstration meeting are carried out to prepare the draft of the standard,and the Delphi method is applied to determine the standard of integrated traditional Chinese and Western medicine care for patients with COVID‑19(mild and common)in Beijing.Results:The nursing standard of integrated traditional Chinese and Western medicine for patients with COVID‑19(mild and common)was established,which included 5 first‑level indicators,14 second-level indicators and 60 third‑level indicators.After two rounds of Delphi method,the positive coefficients of experts were 96%and 83%,the authoritative coefficients of experts were 0.89 and 0.91,and the Kendall’s coefficient of concordance(W)of experts were 0.12,0.09,0.10,0.13(P<0.05)and 0.44,0.43,0.37,0.39(P<0.05).Conclusion:The standard of integrated traditional Chinese and Western medicine nursing for patients with COVID‑19(mild and common)in Beijing constructed by the Delphi method is scientific and practical,which provides a reference for clinical application of integrated traditional Chinese and Western medicine nursing to fight against COVID‑19 infection.
基金supported by the National Natural Science Foundation of China(31640001 and T2350005 to C.X.,U21A20148 to X.Z.and C.X.)Ministry of Science and Technology of China(2021ZD0140300 to C.X.)Presidential Foundation of Hefei Institutes of Physical Science,Chinese Academy of Sciences(Y96XC11131,E26CCG27,and E26CCD15 to C.X.,E36CWGBR24B and E36CZG14132 to T.C.)。
文摘Birds exhibit extraordinary mobility and remarkable navigational skills,obtaining guidance cues from the Earth’s magnetic field for orientation and long-distance movement.Bird species also show tremendous diversity in navigation strategies,with considerable differences even within the same taxa and among individuals from the same population.The highly conserved iron and iron-sulfur cluster binding magnetoreceptor(MagR)protein is suggested to enable animals,including birds,to detect the geomagnetic field and navigate accordingly.Notably,MagR is also implicated in other functions,such as electron transfer and biogenesis of iron-sulfur clusters,raising the question of whether variability exists in its biochemical and biophysical features among species,particularly birds.In the current study,we conducted a comparative analysis of MagR from two different bird species,including the migratory European robin(Erithacus rubecula)and the homing pigeon(Columba livia).Sequence alignment revealed an extremely high degree of similarity between the MagRs of these species,with only three sequence variations.Nevertheless,two of these variations underpinned significant differences in metal binding capacity,oligomeric state,and magnetic properties.These findings offer compelling evidence for the marked differences in MagR between the two avian species,potentially explaining how a highly conserved protein can mediate such diverse functions.
