High-order Laguerre–Gaussian(LG)petal-like beams have become a topic of significant interest due to their potential application in next-generation optical trapping,quantum optics,and materials processing technologies...High-order Laguerre–Gaussian(LG)petal-like beams have become a topic of significant interest due to their potential application in next-generation optical trapping,quantum optics,and materials processing technologies.In this work,we demonstrate the generation of high-order LG beams with petal-like spatial profiles and tunable orbital angular momentum(OAM)in the mid-infrared wavelength region.These beams are generated using idler-resonant optical parametric oscillation(OPO)in a KTiOAsO_(4)(KTA)crystal.By adjusting the length of the resonant cavity,the OAM of the mid-infrared idler field can be tuned and we demonstrate tuning in the range of 0 to10.When using a maximum pump energy of 20.2 mJ,the maximum output energy of high-order modes LG_(0.45),LG_(0.48),and LG_(0.410) were 0.8,0.53,and 0.46 mJ,respectively.The means by which high-order LG modes with petal-like spatial profiles and tunable OAM were generated from the OPO is theoretically modeled by examining the spatial overlap efficiency of the beam waists of the pump and resonant idler fields within the center of the KTA crystal.The methodology presented in this work offers a simple and flexible method to wavelength-convert laser emission and generate high-order LG modes.展开更多
With the people's lives improving,the demands of livestock products on the import and export aspects were growing. Veterinary drug residues as the important factor which might influence animal products safety has ...With the people's lives improving,the demands of livestock products on the import and export aspects were growing. Veterinary drug residues as the important factor which might influence animal products safety has become a major concern. In this paper,the status quo and inspection of the veterinary drug residue and the corresponding control measures were put forward.展开更多
CD4^(+)T cells are essential for sustaining CD8^(+)T cell responses during a chronic infection.The adoptive transfer of virus-specific CD4^(+)T cells has been shown to efficiently rescue exhausted CD8^(+)T cells.Howev...CD4^(+)T cells are essential for sustaining CD8^(+)T cell responses during a chronic infection.The adoptive transfer of virus-specific CD4^(+)T cells has been shown to efficiently rescue exhausted CD8^(+)T cells.However,the question of whether endogenous virus-specific CD4^(+)T cell responses can be enhanced by certain vaccination strategies and subsequently reinvigorate exhausted CD8^(+)T cells remains unexplored.In this study,we developed a CD4^(+)T cell epitope-based heterologous prime-boost immunization strategy and examined the efficacy of this strategy using a mouse model of chronic lymphocytic choriomeningitis virus(LCMV)infection.We primed chronically LCMV-infected mice with a Listeria monocytogenes vector that expressed the LCMV glycoprotein-specific I-Ab-restricted CD4^(+)T cell epitope GP61–80(LM-GP61)and subsequently boosted the primed mice with an influenza virus A(PR8 strain)vector that expressed the same CD4^(+)T cell epitope(IAV-GP61).This heterologous prime-boost vaccination strategy elicited strong anti-viral CD4^(+)T cell responses,which further improved both the quantity and quality of the virusspecific CD8^(+)T cells and led to better control of the viral loads.The combination of this strategy and the blockade of the programmed cell death-1(PD-1)inhibitory pathway further enhanced the anti-viral CD8^(+)T cell responses and viral clearance.Thus,a heterologous prime-boost immunization that selectively induces virus-specific CD4^(+)T cell responses in conjunction with blockade of the inhibitory pathway may represent a promising therapeutic approach to treating patients with chronic viral infections.展开更多
As humans continue to develop COVID-19 widely,numerous novel variants of SARS-CoV-2 have emerged[1,2].These variants,which may possess enhanced transmissibility and often result in breakthrough infections in the vacci...As humans continue to develop COVID-19 widely,numerous novel variants of SARS-CoV-2 have emerged[1,2].These variants,which may possess enhanced transmissibility and often result in breakthrough infections in the vaccinated population,pose great challenges to the current vaccine strategies targeting the immunodominance of the receptor-binding domain(RBD)of the spike(S)protein[2].展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.12264049 and 11664041)the Xinjiang Normal University Young Outstanding Talent Programme(Grant No.XJNUQB2022-17).
文摘High-order Laguerre–Gaussian(LG)petal-like beams have become a topic of significant interest due to their potential application in next-generation optical trapping,quantum optics,and materials processing technologies.In this work,we demonstrate the generation of high-order LG beams with petal-like spatial profiles and tunable orbital angular momentum(OAM)in the mid-infrared wavelength region.These beams are generated using idler-resonant optical parametric oscillation(OPO)in a KTiOAsO_(4)(KTA)crystal.By adjusting the length of the resonant cavity,the OAM of the mid-infrared idler field can be tuned and we demonstrate tuning in the range of 0 to10.When using a maximum pump energy of 20.2 mJ,the maximum output energy of high-order modes LG_(0.45),LG_(0.48),and LG_(0.410) were 0.8,0.53,and 0.46 mJ,respectively.The means by which high-order LG modes with petal-like spatial profiles and tunable OAM were generated from the OPO is theoretically modeled by examining the spatial overlap efficiency of the beam waists of the pump and resonant idler fields within the center of the KTA crystal.The methodology presented in this work offers a simple and flexible method to wavelength-convert laser emission and generate high-order LG modes.
基金Supported by Scientific and Technological Program in State Quality Detection Bureau(2088IK013,2010IK004)Key Scientic techndogical Program(2012ZRZ014,2012SZZ030)
文摘With the people's lives improving,the demands of livestock products on the import and export aspects were growing. Veterinary drug residues as the important factor which might influence animal products safety has become a major concern. In this paper,the status quo and inspection of the veterinary drug residue and the corresponding control measures were put forward.
基金The work was supported by National Basic Research Program of China(973 program,2013CB531500,to LY)the National Natural Science Foundation of China(81471624 to LY).
文摘CD4^(+)T cells are essential for sustaining CD8^(+)T cell responses during a chronic infection.The adoptive transfer of virus-specific CD4^(+)T cells has been shown to efficiently rescue exhausted CD8^(+)T cells.However,the question of whether endogenous virus-specific CD4^(+)T cell responses can be enhanced by certain vaccination strategies and subsequently reinvigorate exhausted CD8^(+)T cells remains unexplored.In this study,we developed a CD4^(+)T cell epitope-based heterologous prime-boost immunization strategy and examined the efficacy of this strategy using a mouse model of chronic lymphocytic choriomeningitis virus(LCMV)infection.We primed chronically LCMV-infected mice with a Listeria monocytogenes vector that expressed the LCMV glycoprotein-specific I-Ab-restricted CD4^(+)T cell epitope GP61–80(LM-GP61)and subsequently boosted the primed mice with an influenza virus A(PR8 strain)vector that expressed the same CD4^(+)T cell epitope(IAV-GP61).This heterologous prime-boost vaccination strategy elicited strong anti-viral CD4^(+)T cell responses,which further improved both the quantity and quality of the virusspecific CD8^(+)T cells and led to better control of the viral loads.The combination of this strategy and the blockade of the programmed cell death-1(PD-1)inhibitory pathway further enhanced the anti-viral CD8^(+)T cell responses and viral clearance.Thus,a heterologous prime-boost immunization that selectively induces virus-specific CD4^(+)T cell responses in conjunction with blockade of the inhibitory pathway may represent a promising therapeutic approach to treating patients with chronic viral infections.
基金This study was supported by the Key Laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin)for Agrifood Safety and Quality(26116120)Social Development Project of the Yangzhou Science and Technology(YZ2020122),the Research Foundation for Talented Scholars in Yangzhou University and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘As humans continue to develop COVID-19 widely,numerous novel variants of SARS-CoV-2 have emerged[1,2].These variants,which may possess enhanced transmissibility and often result in breakthrough infections in the vaccinated population,pose great challenges to the current vaccine strategies targeting the immunodominance of the receptor-binding domain(RBD)of the spike(S)protein[2].
