Alfalfa (Medicago sativa L.) is the most widely cultivated perennial leguminous forage crop woldwide.Micro RNA156 (miR156) precursor genes from dicotyledonous species are reportedly useful for improving alfalfa plant ...Alfalfa (Medicago sativa L.) is the most widely cultivated perennial leguminous forage crop woldwide.Micro RNA156 (miR156) precursor genes from dicotyledonous species are reportedly useful for improving alfalfa plant architecture and abiotic stress resistance.However,there has been no report on whether a miR156 precursor gene from a monocotyledonous species functions in alfalfa.We introduced two tandem precursor genes of miR156,rice Osa-MIR156b and Osa-MIR156c (Osa-MIR156bc),into alfalfa.The expression of miR156 in the transgenic (TG) alfalfa was significantly elevated.Compared to wild-type plants,the TG plants overexpressing miR156 had more branches and leaves and showed improved salt and drought tolerance.Overexpression of miR156 slightly reduced plant height,but the biomass yield of TG plants grown in flowerpots was still increased.Forage quality of TG plants was markedly improved by reduction of acid detergent lignin (ADL) content and increase in crude protein content.The expression of the putative miR156 target genes Ms SPL6,Ms SPL12,and Ms SPL13 in TG plants was repressed by miR156overexpression,and that of all tested Ms SPLs would be sharply increased under drought or salt stress.RNA sequencing revealed that overexpression of miR156 affected the expression of genes associated with abiotic stress resistance and plant development in multiple pathways.This first report of overexpression of monocot miR156 precursors in alfalfa sheds light on the function of mi RNA156 precursors from the monocot species rice that could be used for genetic improvement of the dicot forage crop alfalfa.展开更多
基金supported by the National Natural Science Foundation of China (31971755)the Beijing Municiple Natural Science Foundation (6192011) of China。
文摘Alfalfa (Medicago sativa L.) is the most widely cultivated perennial leguminous forage crop woldwide.Micro RNA156 (miR156) precursor genes from dicotyledonous species are reportedly useful for improving alfalfa plant architecture and abiotic stress resistance.However,there has been no report on whether a miR156 precursor gene from a monocotyledonous species functions in alfalfa.We introduced two tandem precursor genes of miR156,rice Osa-MIR156b and Osa-MIR156c (Osa-MIR156bc),into alfalfa.The expression of miR156 in the transgenic (TG) alfalfa was significantly elevated.Compared to wild-type plants,the TG plants overexpressing miR156 had more branches and leaves and showed improved salt and drought tolerance.Overexpression of miR156 slightly reduced plant height,but the biomass yield of TG plants grown in flowerpots was still increased.Forage quality of TG plants was markedly improved by reduction of acid detergent lignin (ADL) content and increase in crude protein content.The expression of the putative miR156 target genes Ms SPL6,Ms SPL12,and Ms SPL13 in TG plants was repressed by miR156overexpression,and that of all tested Ms SPLs would be sharply increased under drought or salt stress.RNA sequencing revealed that overexpression of miR156 affected the expression of genes associated with abiotic stress resistance and plant development in multiple pathways.This first report of overexpression of monocot miR156 precursors in alfalfa sheds light on the function of mi RNA156 precursors from the monocot species rice that could be used for genetic improvement of the dicot forage crop alfalfa.
