Background:Renal sympathetic nerves are involved in the reflective activation of the sympathetic nervous system in circulatory control.Catheter-based renal denervation (RDN) ameliorated treatment-resistant hypertensio...Background:Renal sympathetic nerves are involved in the reflective activation of the sympathetic nervous system in circulatory control.Catheter-based renal denervation (RDN) ameliorated treatment-resistant hypertension safely,but 10％-20％ of treated patients are nonresponders to radiofrequency denervation.The purpose of this study was to investigate the safety and efficiency of cryoablation for sympathetic denervation in a swine model and to explore a new way of RDN.Methods:Seven swines randomly assigned to two groups:Renal cryoablation (CR) group and control group.The control group underwent renal angiogram only.The CR group underwent renal angiogram plus bilateral renal cryoablation.Renal angiograms via femoral were performed before denervation,after denervation and prior to the sacrifice to access the diameter of renal arterial and the pressure of aorta abdominalis.Euthanasia of the swine was performed on 28-day to access norepinephrine (NE) changes of the renal cortex and the changes of renal nerves.Results:Cryoablation did not induce severe complications at any time point.There was no significant change in diameter of renal artery.CR reduced systolic blood pressure (BP) from 145.50 ± 9.95 mmHg at baseline to 119.00 ± 14.09 mmHg.There was a slight but insignificant decrease in diastolic BP.The main nerve changes at 28-day consisted of necrosis with perineurial fibrosis at the site of CR exposure in conjunction with the nerve vacuolation.Compared with the control group,renal tissue NE of CR group decreased by 89.85％.Conclusions:Percutaneous catheter-based cryoablation of the renal artery is safe.CR could effectively reduce NE storing in the renal cortex,and the efficiency could be maintained 28-day at least.展开更多
Background:Growing industrialization of China exposes its labor population to the risk of musculoskeletal disorders(MSDs).This study aimed to investigate the prevalence and risk factors of MSDs in a modern industrial ...Background:Growing industrialization of China exposes its labor population to the risk of musculoskeletal disorders(MSDs).This study aimed to investigate the prevalence and risk factors of MSDs in a modern industrial region of Beijing.Methods:A cross-sectional study included 1415 employees in six industrial companies was conducted between January 2018 and May 2018 in Fangshan district,Beijng,China.Nordic Musculoskeletal Questionnaire(NMQ)was used to collect the information about MSDs.Demographic factors,lifestyle factors,health and medical factors,and work-related factors were collected as independent variables.Descriptive statistics,the chi-squared(/2)test,and binary logistic regression analysis were used to analyze data.Results:Among 1415 participants,498 reported MSDs.The regions involved were the neck(25.16%),shoulders(17.17%),and upper back(13.29%).There was a significant statistical difference between frontline industrial workers and other staff in the prevalence of self-reported symptoms involving the shoulders(Х^2=4.33,P=0.037),wrists and hands(Х^2=8.90,P=0.003),and ankles and feet(Х^2=12.88,P<0.001).Increased age(P=0.005,OR=1.63;P=0.001,OR=2.33),a high or a low salary(P<0.001,OR=0.49;P<0.001,OR=0.30),night-shift(P=0.027,OR=1.46),two-week-history of illness and treatment(P=0.004,OR=5.60;P=0.013,OR=4.19),concurrent chronic diseases(P=0.001,OR=3.45;P=0.092,OR=7.81),limited access to health information(P=0.004,OR=0.49),and negative attitude towards seeking healthcare(P=0.010,OR=1.77;P=0.009,OR=2.75)were associated with MSDs in frontline workers.Female gender(P<0.001,OR=2.30),high education(P=0.001,OR=1.96),no exercises(P=0.027,OR=0.59),night-shift(P=0.017,OR=1.98),concurrent chronic diseases(P=0.002,OR=3.73;P=0.020,OR=13.42),limited access to health information(P=0.013,OR=0.53),far distance to medical institution(P=0.009,OR=1.83),and negative propensity(P=0.009,OR=1.94;P=0.014,OR=2.74)were associated with MSDs in other staffs.Conclusions:The prevalence of MSDs among industrial employees has changed.Frontline workers had different prevalence and risk factors for MSDs compared with other employees.Negative propensity to healthcare,limited ways to obtain health knowledge,and concomitant chronic diseases were associated with MSDs.Surprisingly,highly educated and high-income employees had a higher risk of MSDs.展开更多
Background： Coronary microembolization （CME） has been frequently seen in acute coronary syndromes and percutaneous coronary intervention. Small animal models are required for further studies of CME related to sever...Background： Coronary microembolization （CME） has been frequently seen in acute coronary syndromes and percutaneous coronary intervention. Small animal models are required for further studies of CME related to severe prognosis. This study aimed to explore a new mouse model of CME. Methods： The mouse model of CME was established by injecting polystyrene microspheres into the left ventricular chamber during 15-s occlusion of the ascending aorta. Based on the average diameter and dosage used, 30 C57BL/6 male mice were randomly divided into five groups （n = 6 in each）： 9 μm/500,000, 9 μm/800,000, 17 μm/200,000, 17 μm/500,000, and sham groups. The postoperative survival and performance of the mice were recorded. The mice were sacrificed 3 or 10 days after the surgery. The heart tissues were harvested for hematoxylin and eosin staining and Masson trichrome staining to compare the extent of inflammatory cellular infiltration and fibrin deposition among groups and for scanning transmission electron microscopic examinations to see the ultrastructural changes after CME. Results： Survival analysis demonstrated that the cumulative survival rate of the 17 μm/500,000 group was significantly lower than that of the sham group （0/6 vs. 6/6, P = 0.001）. The cumulative survival rate of the 17 μm/200,000 group was lower than those of the sham and 9 μm groups with no statistical difference （cumulative survival rate of the 17 μm/200,000, 9 μm/800,000, 9 μm/500,000, and sham groups was 4/6, 5/6, 6/6, and 6/6, respectively）. The pathological alterations were similar between the 9 μm/500,000 and 9 μm/800,000 groups. The extent of inflammatory cellular infiltration and fibrin deposition was more severe in the 17 μm/200,000 group than in the 9 μm/500,000 and 9 μm/800,000 groups 3 and 10 days after the surgery. Scanning transmission electron microscopic examinations revealed platelet aggregation and adhesion, microthrombi formation, and changes in cardiomyocytes. Conclusion： The injection of 500,000 polystyrene microspheres at an average diameter of 9 μm is proved to be appropriate for the mouse model of CME based on the general conditions, postoperative survival rates, and pathological changes.展开更多
基金grants from National Natural Science Foundation of China，the National Basic Research Program of China (No.2011CB503905) from the Ministry of Science and Technology of China
文摘Background:Renal sympathetic nerves are involved in the reflective activation of the sympathetic nervous system in circulatory control.Catheter-based renal denervation (RDN) ameliorated treatment-resistant hypertension safely,but 10％-20％ of treated patients are nonresponders to radiofrequency denervation.The purpose of this study was to investigate the safety and efficiency of cryoablation for sympathetic denervation in a swine model and to explore a new way of RDN.Methods:Seven swines randomly assigned to two groups:Renal cryoablation (CR) group and control group.The control group underwent renal angiogram only.The CR group underwent renal angiogram plus bilateral renal cryoablation.Renal angiograms via femoral were performed before denervation,after denervation and prior to the sacrifice to access the diameter of renal arterial and the pressure of aorta abdominalis.Euthanasia of the swine was performed on 28-day to access norepinephrine (NE) changes of the renal cortex and the changes of renal nerves.Results:Cryoablation did not induce severe complications at any time point.There was no significant change in diameter of renal artery.CR reduced systolic blood pressure (BP) from 145.50 ± 9.95 mmHg at baseline to 119.00 ± 14.09 mmHg.There was a slight but insignificant decrease in diastolic BP.The main nerve changes at 28-day consisted of necrosis with perineurial fibrosis at the site of CR exposure in conjunction with the nerve vacuolation.Compared with the control group,renal tissue NE of CR group decreased by 89.85％.Conclusions:Percutaneous catheter-based cryoablation of the renal artery is safe.CR could effectively reduce NE storing in the renal cortex,and the efficiency could be maintained 28-day at least.
文摘Background:Growing industrialization of China exposes its labor population to the risk of musculoskeletal disorders(MSDs).This study aimed to investigate the prevalence and risk factors of MSDs in a modern industrial region of Beijing.Methods:A cross-sectional study included 1415 employees in six industrial companies was conducted between January 2018 and May 2018 in Fangshan district,Beijng,China.Nordic Musculoskeletal Questionnaire(NMQ)was used to collect the information about MSDs.Demographic factors,lifestyle factors,health and medical factors,and work-related factors were collected as independent variables.Descriptive statistics,the chi-squared(/2)test,and binary logistic regression analysis were used to analyze data.Results:Among 1415 participants,498 reported MSDs.The regions involved were the neck(25.16%),shoulders(17.17%),and upper back(13.29%).There was a significant statistical difference between frontline industrial workers and other staff in the prevalence of self-reported symptoms involving the shoulders(Х^2=4.33,P=0.037),wrists and hands(Х^2=8.90,P=0.003),and ankles and feet(Х^2=12.88,P<0.001).Increased age(P=0.005,OR=1.63;P=0.001,OR=2.33),a high or a low salary(P<0.001,OR=0.49;P<0.001,OR=0.30),night-shift(P=0.027,OR=1.46),two-week-history of illness and treatment(P=0.004,OR=5.60;P=0.013,OR=4.19),concurrent chronic diseases(P=0.001,OR=3.45;P=0.092,OR=7.81),limited access to health information(P=0.004,OR=0.49),and negative attitude towards seeking healthcare(P=0.010,OR=1.77;P=0.009,OR=2.75)were associated with MSDs in frontline workers.Female gender(P<0.001,OR=2.30),high education(P=0.001,OR=1.96),no exercises(P=0.027,OR=0.59),night-shift(P=0.017,OR=1.98),concurrent chronic diseases(P=0.002,OR=3.73;P=0.020,OR=13.42),limited access to health information(P=0.013,OR=0.53),far distance to medical institution(P=0.009,OR=1.83),and negative propensity(P=0.009,OR=1.94;P=0.014,OR=2.74)were associated with MSDs in other staffs.Conclusions:The prevalence of MSDs among industrial employees has changed.Frontline workers had different prevalence and risk factors for MSDs compared with other employees.Negative propensity to healthcare,limited ways to obtain health knowledge,and concomitant chronic diseases were associated with MSDs.Surprisingly,highly educated and high-income employees had a higher risk of MSDs.
文摘Background： Coronary microembolization （CME） has been frequently seen in acute coronary syndromes and percutaneous coronary intervention. Small animal models are required for further studies of CME related to severe prognosis. This study aimed to explore a new mouse model of CME. Methods： The mouse model of CME was established by injecting polystyrene microspheres into the left ventricular chamber during 15-s occlusion of the ascending aorta. Based on the average diameter and dosage used, 30 C57BL/6 male mice were randomly divided into five groups （n = 6 in each）： 9 μm/500,000, 9 μm/800,000, 17 μm/200,000, 17 μm/500,000, and sham groups. The postoperative survival and performance of the mice were recorded. The mice were sacrificed 3 or 10 days after the surgery. The heart tissues were harvested for hematoxylin and eosin staining and Masson trichrome staining to compare the extent of inflammatory cellular infiltration and fibrin deposition among groups and for scanning transmission electron microscopic examinations to see the ultrastructural changes after CME. Results： Survival analysis demonstrated that the cumulative survival rate of the 17 μm/500,000 group was significantly lower than that of the sham group （0/6 vs. 6/6, P = 0.001）. The cumulative survival rate of the 17 μm/200,000 group was lower than those of the sham and 9 μm groups with no statistical difference （cumulative survival rate of the 17 μm/200,000, 9 μm/800,000, 9 μm/500,000, and sham groups was 4/6, 5/6, 6/6, and 6/6, respectively）. The pathological alterations were similar between the 9 μm/500,000 and 9 μm/800,000 groups. The extent of inflammatory cellular infiltration and fibrin deposition was more severe in the 17 μm/200,000 group than in the 9 μm/500,000 and 9 μm/800,000 groups 3 and 10 days after the surgery. Scanning transmission electron microscopic examinations revealed platelet aggregation and adhesion, microthrombi formation, and changes in cardiomyocytes. Conclusion： The injection of 500,000 polystyrene microspheres at an average diameter of 9 μm is proved to be appropriate for the mouse model of CME based on the general conditions, postoperative survival rates, and pathological changes.
