gallbladder cancer(gbc), although considered as a relatively rare malignancy, is the most common neoplasm of the biliary tract system. the late diagnosis and abysmal prognosis present challenges to treatment. the over...gallbladder cancer(gbc), although considered as a relatively rare malignancy, is the most common neoplasm of the biliary tract system. the late diagnosis and abysmal prognosis present challenges to treatment. the overall 5-year survival rate for metastatic gbc patients is extremely low. BRC A1 and BRCA2 are the breast cancer susceptibility genes and their mutation carriers are at a high risk for cancer development, both in men and women. Olaparib, an oral poly ADP-ribose polymerase inhibitor, has been approved by the Food and Drug Administration and the European commission for the treatment of ovarian cancer with any BRCA1/2 mutations. the first case of BRCA1-mutated gbc patient who responded to olaparib treatment is reported here.展开更多
Neural progenitor cells(NPCs) capable of self-renewal and differentiation into neural cell lineages offer broad prospects for cell therapy for neurodegenerative diseases. However, cell therapy based on NPC transplanta...Neural progenitor cells(NPCs) capable of self-renewal and differentiation into neural cell lineages offer broad prospects for cell therapy for neurodegenerative diseases. However, cell therapy based on NPC transplantation is limited by the inability to acquire sufficient quantities of NPCs. Previous studies have found that a chemical cocktail of valproic acid, CHIR99021, and Repsox(VCR) promotes mouse fibroblasts to differentiate into NPCs under hypoxic conditions. Therefore, we used VCR(0.5 mM valproic acid, 3 μM CHIR99021, and 1 μM Repsox) to induce the reprogramming of rat embryonic fibroblasts into NPCs under a hypoxic condition(5%). These NPCs exhibited typical neurosphere-like structures that can express NPC markers, such as Nestin, SRY-box transcription factor 2, and paired box 6(Pax6), and could also differentiate into multiple types of functional neurons and astrocytes in vitro. They had similar gene expression profiles to those of rat brain-derived neural stem cells. Subsequently, the chemically-induced NPCs(ciNPCs) were stereotactically transplanted into the substantia nigra of 6-hydroxydopamine-lesioned parkinsonian rats. We found that the ciNPCs exhibited long-term survival, migrated long distances, and differentiated into multiple types of functional neurons and glial cells in vivo. Moreover, the parkinsonian behavioral defects of the parkinsonian model rats grafted with ciNPCs showed remarkable functional recovery. These findings suggest that rat fibroblasts can be directly transformed into NPCs using a chemical cocktail of VCR without introducing exogenous factors, which may be an attractive donor material for transplantation therapy for Parkinson’s disease.展开更多
Intrahepatic cholangiocarcinoma(ICC) is a relatively rare form of liver cancer with a poor prognosis. The therapeutic options for patients with advanced ICC are limited and usually ineffective. There is currently no a...Intrahepatic cholangiocarcinoma(ICC) is a relatively rare form of liver cancer with a poor prognosis. The therapeutic options for patients with advanced ICC are limited and usually ineffective. There is currently no approved targeted therapy for ICC, although accumulating evidence supports inhibition of the PI3K/Akt/m TOR signaling pathway as a promising therapeutic strategy in the treatment of ICC. Here, we report a patient with stage IV ICC harboring a PIK3 CA mutation who responded well to the m TOR inhibitor everolimus. Computed tomography and magnetic resonance imaging demonstrated shrinkage of the tumor and maintenance of a partial response for 6.5 mo after everolimus treatment as the best response. To the best of our knowledge, this is the first clinical case report in the literature of clinical benefit from everolimus treatment in an ICC patient with PIK3 CA mutation.展开更多
Over the past decade,energy-related carbon dioxide(CO_(2))emissions have steadily increased worldwide,reaching 33.4 billion tons in 2019.Most climate scenarios suggest that carbon capture,utilization,and storage(CCUS)...Over the past decade,energy-related carbon dioxide(CO_(2))emissions have steadily increased worldwide,reaching 33.4 billion tons in 2019.Most climate scenarios suggest that carbon capture,utilization,and storage(CCUS)is critical for the reduction of direct CO_(2)emissions from industrial processes and the use of fossil fuels in power generation,industrial activities,and fuel conversion.Consequently.展开更多
Chronic pancreatitis(CP)is a progressive and irreversible fibroinflammatory disorder,accompanied by pancreatic exocrine insufficiency and dysregulated gut microbiota.Recently,accumulating evidence has supported a corr...Chronic pancreatitis(CP)is a progressive and irreversible fibroinflammatory disorder,accompanied by pancreatic exocrine insufficiency and dysregulated gut microbiota.Recently,accumulating evidence has supported a correlation between gut dysbiosis and CP development.However,whether gut microbiota dysbiosis contributes to CP pathogenesis remains unclear.Herein,an experimental CP was induced by repeated high-dose caerulein injections.The broad-spectrum antibiotics(ABX)and ABX targeting Gram-positive(G+)or Gram-negative bacteria(G-)were applied to explore the specific roles of these bacteria.Gut dysbiosis was observed in both mice and in CP patients,which was accompanied by a sharply reduced abundance for short-chain fatty acids(SCFAs)-producers,especially G+bacteria.Broad-spectrum ABX exacerbated the severity of CP,as evidenced by aggravated pancreatic fibrosis and gut dysbiosis,especially the depletion of SCFAs-producing G+bacteria.Additionally,depletion of SCFAs-producing G+bacteria rather than G-bacteria intensified CP progression independent of TLR4,which was attenuated by supplementation with exogenous SCFAs.Finally,SCFAs modulated pancreatic fibrosis through inhibition of macrophage infiltration and M2 phenotype switching.The study supports a critical role for SCFAs-producing G+bacteria in CP.Therefore,modulation of dietary-derived SCFAs or G+SCFAs-producing bacteria may be considered a novel interventive approach for the management of CP.展开更多
Introduction Lymphatic malformations (LMs) are composed of dilated, abnormal lymphatic channels of varying size that can result in significant physical and esthetic impairment due to relentless growth(1)LMs represent ...Introduction Lymphatic malformations (LMs) are composed of dilated, abnormal lymphatic channels of varying size that can result in significant physical and esthetic impairment due to relentless growth(1)LMs represent a group of heterogeneous diseases, and are classi if ed into three types: microcystic/superficial LM, which was named as lymphangioma circumscriptum[2], macro-cystic LM, and mixed LMs(2-4)In clinical practice, it is dif if cult to classify LMs because some cases have been found to differ from the well- characterized entities. Cutaneous LMs are uncommon, and usually present as microcystic/superficial LM. This condition represents congenitally determined or developmental malformations of the superficial lymphatics rather than true neoplasms. Although developmental dys-plasia, these lesions do not appear genetic, as familial cases do not seem to occur. Typically, LM in infancy usually has the highest incidence of onset, but the lesions can spontaneously appear in adolescence or adulthood, and mostly by the age of 30 years(1- 2,4)Herein we report a case of late-onset superficial LM, which is relatively infrequent.展开更多
基金supported by International Science and Technology Cooperation Projects,No.2015DFA30650 and No.2010DFB33720Capital Special Research Project for Health Development,No.2014-2-4012Capital Research Project for the Characteristics Clinical Application,No.Z151100004015170
文摘gallbladder cancer(gbc), although considered as a relatively rare malignancy, is the most common neoplasm of the biliary tract system. the late diagnosis and abysmal prognosis present challenges to treatment. the overall 5-year survival rate for metastatic gbc patients is extremely low. BRC A1 and BRCA2 are the breast cancer susceptibility genes and their mutation carriers are at a high risk for cancer development, both in men and women. Olaparib, an oral poly ADP-ribose polymerase inhibitor, has been approved by the Food and Drug Administration and the European commission for the treatment of ovarian cancer with any BRCA1/2 mutations. the first case of BRCA1-mutated gbc patient who responded to olaparib treatment is reported here.
基金supported by the National Natural Science Foundation of China,No. 81771381 (to CQL)Anhui Provincial Key Research and Development Project,Nos. 2022e07020030 (to CQL), 2022e07020032 (to YG)+2 种基金Science Research Project of Bengbu Medical College,No. 2021byfy002 (to CQL)the Natural Science Foundation of the Higher Education Institutions of Anhui Province,No. KJ2021ZD0085 (to CJW)the Undergraduate Innovative Training Program of China,Nos. 202110367043 (to CQL), 202110367044 (to YG)。
文摘Neural progenitor cells(NPCs) capable of self-renewal and differentiation into neural cell lineages offer broad prospects for cell therapy for neurodegenerative diseases. However, cell therapy based on NPC transplantation is limited by the inability to acquire sufficient quantities of NPCs. Previous studies have found that a chemical cocktail of valproic acid, CHIR99021, and Repsox(VCR) promotes mouse fibroblasts to differentiate into NPCs under hypoxic conditions. Therefore, we used VCR(0.5 mM valproic acid, 3 μM CHIR99021, and 1 μM Repsox) to induce the reprogramming of rat embryonic fibroblasts into NPCs under a hypoxic condition(5%). These NPCs exhibited typical neurosphere-like structures that can express NPC markers, such as Nestin, SRY-box transcription factor 2, and paired box 6(Pax6), and could also differentiate into multiple types of functional neurons and astrocytes in vitro. They had similar gene expression profiles to those of rat brain-derived neural stem cells. Subsequently, the chemically-induced NPCs(ciNPCs) were stereotactically transplanted into the substantia nigra of 6-hydroxydopamine-lesioned parkinsonian rats. We found that the ciNPCs exhibited long-term survival, migrated long distances, and differentiated into multiple types of functional neurons and glial cells in vivo. Moreover, the parkinsonian behavioral defects of the parkinsonian model rats grafted with ciNPCs showed remarkable functional recovery. These findings suggest that rat fibroblasts can be directly transformed into NPCs using a chemical cocktail of VCR without introducing exogenous factors, which may be an attractive donor material for transplantation therapy for Parkinson’s disease.
文摘Intrahepatic cholangiocarcinoma(ICC) is a relatively rare form of liver cancer with a poor prognosis. The therapeutic options for patients with advanced ICC are limited and usually ineffective. There is currently no approved targeted therapy for ICC, although accumulating evidence supports inhibition of the PI3K/Akt/m TOR signaling pathway as a promising therapeutic strategy in the treatment of ICC. Here, we report a patient with stage IV ICC harboring a PIK3 CA mutation who responded well to the m TOR inhibitor everolimus. Computed tomography and magnetic resonance imaging demonstrated shrinkage of the tumor and maintenance of a partial response for 6.5 mo after everolimus treatment as the best response. To the best of our knowledge, this is the first clinical case report in the literature of clinical benefit from everolimus treatment in an ICC patient with PIK3 CA mutation.
基金Supported by the projects of Geoscience Literature Knowledge Service and Decision Support(DD20230139)Natural Resource Information Tracking and Research(DD20221794).
文摘Over the past decade,energy-related carbon dioxide(CO_(2))emissions have steadily increased worldwide,reaching 33.4 billion tons in 2019.Most climate scenarios suggest that carbon capture,utilization,and storage(CCUS)is critical for the reduction of direct CO_(2)emissions from industrial processes and the use of fossil fuels in power generation,industrial activities,and fuel conversion.Consequently.
基金supported by funds from the National Natural Science Foundation of China(Grant Nos.82070666,82122068)the Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province(Grant No.BK20200026,China)+4 种基金the Collaborative Innovation Center of Food Safety and Quality Control of Jiangsu Province,the Fundamental Research Funds for the Central Universities(Grant Nos.JUSRP221037,JUSRP22007,China)the China Postdoctoral Science Foundation(Grant No.2022M721366)the Excellent Postdoctoral Program of Jiangsu Province(Grant No.2023ZB168,China)Wuxi City’s first“double hundred”young and middle-aged medical and health talents(Grant No:BJ2020045,China)Wuxi Social Development Science and Technology Demonstration Project(Grant No:N20201003,China)。
文摘Chronic pancreatitis(CP)is a progressive and irreversible fibroinflammatory disorder,accompanied by pancreatic exocrine insufficiency and dysregulated gut microbiota.Recently,accumulating evidence has supported a correlation between gut dysbiosis and CP development.However,whether gut microbiota dysbiosis contributes to CP pathogenesis remains unclear.Herein,an experimental CP was induced by repeated high-dose caerulein injections.The broad-spectrum antibiotics(ABX)and ABX targeting Gram-positive(G+)or Gram-negative bacteria(G-)were applied to explore the specific roles of these bacteria.Gut dysbiosis was observed in both mice and in CP patients,which was accompanied by a sharply reduced abundance for short-chain fatty acids(SCFAs)-producers,especially G+bacteria.Broad-spectrum ABX exacerbated the severity of CP,as evidenced by aggravated pancreatic fibrosis and gut dysbiosis,especially the depletion of SCFAs-producing G+bacteria.Additionally,depletion of SCFAs-producing G+bacteria rather than G-bacteria intensified CP progression independent of TLR4,which was attenuated by supplementation with exogenous SCFAs.Finally,SCFAs modulated pancreatic fibrosis through inhibition of macrophage infiltration and M2 phenotype switching.The study supports a critical role for SCFAs-producing G+bacteria in CP.Therefore,modulation of dietary-derived SCFAs or G+SCFAs-producing bacteria may be considered a novel interventive approach for the management of CP.
文摘Introduction Lymphatic malformations (LMs) are composed of dilated, abnormal lymphatic channels of varying size that can result in significant physical and esthetic impairment due to relentless growth(1)LMs represent a group of heterogeneous diseases, and are classi if ed into three types: microcystic/superficial LM, which was named as lymphangioma circumscriptum[2], macro-cystic LM, and mixed LMs(2-4)In clinical practice, it is dif if cult to classify LMs because some cases have been found to differ from the well- characterized entities. Cutaneous LMs are uncommon, and usually present as microcystic/superficial LM. This condition represents congenitally determined or developmental malformations of the superficial lymphatics rather than true neoplasms. Although developmental dys-plasia, these lesions do not appear genetic, as familial cases do not seem to occur. Typically, LM in infancy usually has the highest incidence of onset, but the lesions can spontaneously appear in adolescence or adulthood, and mostly by the age of 30 years(1- 2,4)Herein we report a case of late-onset superficial LM, which is relatively infrequent.
