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新型脱细胞动脉基质的制备及理化性能评估 被引量:1
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作者 张增增 郝春香 +12 位作者 郭维民 陈明学 王振勇 张学亮 李旭 荆晓光 刘雪剑 孙百川 郝立波 眭翔 刘舒云 郭全义 周成福 《中国医药生物技术》 2018年第2期130-136,共7页
目的探索新型脱细胞动脉基质的制备方法,并分析评估其各项理化性能。方法采用自主设计的三联脱细胞程序,逐步去除动脉血管中的细胞和抗原成分。通过甲苯胺蓝、HE及天狼猩红染色和羟脯氨酸定量分析,对比脱细胞前后血管基质成分与天然半... 目的探索新型脱细胞动脉基质的制备方法,并分析评估其各项理化性能。方法采用自主设计的三联脱细胞程序,逐步去除动脉血管中的细胞和抗原成分。通过甲苯胺蓝、HE及天狼猩红染色和羟脯氨酸定量分析,对比脱细胞前后血管基质成分与天然半月板之间的差异;并采用Hoechst33258荧光染色法观察脱细胞前后动脉中DNA残留情况;通过MTT法考察材料经脱细胞程序后的毒性强弱以及对细胞活性的影响;将兔半月板细胞接种到脱细胞血管基质后培养,观察两者的相容性。结果三联脱细胞方法制备的牛大动脉基质的组织学染色结果与正常半月板类似,进一步胶原定量证实,材料经脱细胞后胶原含量无明显统计学差异;通过Hoechst33258染色,未发现血管基质中细胞核和DNA残留;甲苯胺蓝及天狼猩红染色证实脱细胞血管基质支架可以很好地保留胶原成分;MTT结果显示脱细胞动脉基质无细胞毒性,具有良好的生物相容性。半月板细胞接种材料后扫描电镜结果显示,脱细胞血管基质能很好促进细胞黏附,并诱导细胞的增殖。结论自主设计的脱细胞程序能完全去除主要抗原成分,无DNA及细胞碎片的残留,并保留了大部分细胞外基质成分、完整组织结构和良好的力学性能,同时具有良好的细胞生物相容性。对比性分析研究得出,在结构和成分上,脱细胞血管基质与半月板具有高度相似性,是未来非常有应用潜力的组织工程半月板支架之一。 展开更多
关键词 细胞外基质 组织工程 半月板修复
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Mechanical and acoustic emission characteristics of anhydrite rock under freeze-thaw cycles 被引量:2
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作者 ZHANG Chi jin xiao-guang +1 位作者 HOU Chao HE Jie 《Journal of Mountain Science》 SCIE CSCD 2023年第1期227-241,共15页
To study the damage mechanisms of anhydrite rock under freeze-thaw cycles, the physicalmechanical properties and the microcracking activities of anhydrite rock were investigated through mass variation, nuclear magneti... To study the damage mechanisms of anhydrite rock under freeze-thaw cycles, the physicalmechanical properties and the microcracking activities of anhydrite rock were investigated through mass variation, nuclear magnetic resonance, scanning electron microscope tests, and uniaxial compression combined with acoustic emission(AE) tests. Results show that with the increase of freeze-thaw processes,the mass, uniaxial compression strength, and elastic modulus of the anhydrite specimens decrease while the porosity and plasticity characteristics increase.For example, after 120 cycles, the uniaxial compression strength and elastic modulus decrease by 46.54% and 60.16%, and the porosity increase by 75%. Combined with the evolution trend of stressstrain curves and the detected events, three stages were labeled to investigate the AE characteristics in freeze-thaw weathered anhydrite rock. It is found that with the increase of freeze-thaw cycles, the proportions of AE counts in stage Ⅰ and stage Ⅱ show a decaying exponential trend. Contrarily, the proportion of AE counts in stage Ⅲ displays an exponential ascending trend. Meanwhile, as the freeze-thaw cycles increase, the low-frequency AE signals increase while the intermediate-frequency AE signals decrease. After 120 cycles, the proportion of low-frequency AE signals increases by 168.95%, and the proportion of intermediate-frequency AE signals reduces by 81.14%. It is concluded that the microtensile cracking events occupy a dominant position during the loading process. With the increase of freeze-thaw cycles, the b value of samples decreases.After 120 cycles, b value decreases by 27.2%, which means that the proportion of cracking events in rocks with small amplitude decreases. Finally, it is proposed that the freeze-thaw damage mechanism of anhydrite is also characterized by the water chemical softening effect. 展开更多
关键词 Freeze-thaw cycles Anhydrite rock Physical and mechanical properties AE characteristics Damage mechanism
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A simplified testing method for stress-induced of tunnel surrounding rock
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作者 jin xiao-guang WANG Xing-jiu +1 位作者 LI Xiao-hong XU jin 《Journal of Coal Science & Engineering(China)》 2010年第3期225-229,共5页
A simple testing method for secondary or induced stress of surrounding rockwas presented by laboratory experiment, numerical simulation and in situ testing based onthe basic principle of the historical stress restorin... A simple testing method for secondary or induced stress of surrounding rockwas presented by laboratory experiment, numerical simulation and in situ testing based onthe basic principle of the historical stress restoring method.First, stress equivalent coefficient,which key coefficient of stress restored testing, was obtained by laboratory experiment.Second, experimental results were examined using 3D finite element numericalanalysis and the influence factors were analyzed by 2D finite element numerical analysis.The correctness of induced stress measuring results in situ for highway tunnel wereproved by elastic mechanics theory solution and single-hole stress rescission method.Thenew simple method of induced stress measuring has important practical value for undergroundengineering induced stress field analysis. 展开更多
关键词 stress-induced stress measurement numerical analysis EXPERIMENT
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Study on optimization of underlying coal bed exploited depth for tunnel
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作者 jin xiao-guang DING Bao-chen LI Xiao-hong 《Journal of Coal Science & Engineering(China)》 2010年第1期11-16,共6页
Combined with highway construction, the analysis on the relationship betweentunnel construction and coal resource exploitation was processed, which was based onthe research of rational exploitation depth of coal.3D FE... Combined with highway construction, the analysis on the relationship betweentunnel construction and coal resource exploitation was processed, which was based onthe research of rational exploitation depth of coal.3D FEM numerical analysis for tunnelexcavation was carried out according to engineering geological features of coal measurestrata in the project area.Based on the analysis of displacement and stress of the surroundingrock in the tunnel after excavation, the characteristics for displacement andstress of the tunnel support structure were analyzed when the underlying coal bed wasexploited with sublevel and full caving method.In addition, combined with the related codeand standard, the economic and safe prohibiting exploited depth of the underlying coalbed was proposed, so that a scientific basis for tunnel construction of coal measure strataand reasonable exploitation of the mineral resources in complex geological conditions canbe offered. 展开更多
关键词 deep buried tunnel underlying coal bed prohibiting exploited depth structura stability
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掌侧入路切开复位解剖型锁定钢板内固定治疗干骺端掌侧粉碎的不稳定C2型桡骨远端骨折 被引量:22
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作者 马梓元 马明 +9 位作者 高峰 王彬 陈诚 金小广 苏日宝 孙泽毅 周小小 易存国 王秀会 夏胜利 《中国骨与关节损伤杂志》 2022年第3期262-266,共5页
目的探讨掌侧入路切开复位解剖型锁定钢板内固定治疗干骺端掌侧粉碎的不稳定C2型桡骨远端骨折的临床疗效。方法回顾性分析自2015-01—2019-12采用掌侧入路切开复位解剖型锁定钢板内固定治疗的59例干骺端掌侧粉碎的不稳定C2型桡骨远端骨... 目的探讨掌侧入路切开复位解剖型锁定钢板内固定治疗干骺端掌侧粉碎的不稳定C2型桡骨远端骨折的临床疗效。方法回顾性分析自2015-01—2019-12采用掌侧入路切开复位解剖型锁定钢板内固定治疗的59例干骺端掌侧粉碎的不稳定C2型桡骨远端骨折,术中采用“复位-锁定近端-再复位-锁定远端”的治疗策略。结果59例均顺利完成手术并获得至少12个月的随访,切口均一期愈合,术后出现1例切口红肿,1例因螺钉过长导致出现伸肌腱刺激症状,1例远端锁钉穿出关节面软骨,相关并发症症状均经对症处理后改善。术后3个月复查X线片提示骨折均愈合良好,下尺桡关节对位良好,未见骨折复位明显丢失。桡骨解剖学参数、腕关节活动度恢复良好,术后1年DASH评分6~30分,平均14.86分;PRWE疼痛与功能评分4~30分,平均15.95分;Bartra放射学评分70~100分,平均87.95分;采用GarlandWerley腕关节功能评分评价疗效:优32例,良21例,可6例,优良率89.8%。结论掌侧入路切开复位解剖型锁定钢板内固定治疗干骺端掌侧粉碎的不稳定C2型桡骨远端骨折,能获得满意的内固定效果以及良好的腕关节功能,是一种安全有效的手术方法。 展开更多
关键词 桡骨远端骨折 干骺端掌侧粉碎 切开复位 解剖型锁定钢板 内固定
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基于多模态的半桥LLC拓扑ZVS条件分析 被引量:1
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作者 万兰若 刘威 +2 位作者 靳晓光 徐乃珺 吕征宇 《电子技术(上海)》 2018年第5期22-26,共5页
针对传统半桥LLC拓扑只在谐振点附近建模来进行变压器原边零电压开通(ZVS)条件分析,并以此设计谐振腔参数,导致LLC宽范围工作时其余模态不能保证一次侧MOS管零电压开通(ZVS)的问题,对基于多模态下的半桥LLC拓扑的ZVS条件进行了研究,开... 针对传统半桥LLC拓扑只在谐振点附近建模来进行变压器原边零电压开通(ZVS)条件分析,并以此设计谐振腔参数,导致LLC宽范围工作时其余模态不能保证一次侧MOS管零电压开通(ZVS)的问题,对基于多模态下的半桥LLC拓扑的ZVS条件进行了研究,开展了半桥LLC拓扑在五种常见模态下死区时间内基于能量角度的分析,提出了更精确的ZVS判断条件,克服了传统ZVS条件不适用于电路宽范围工作的缺点。利用仿真软件Simetrix&Simplis搭建了电路仿真模型,以一个重载工作点为例验证了新条件相较传统ZVS条件更精确的特点。搭建了谐振频率100k Hz,额定功率330W的实验平台并对该拓扑在高压重载下的ZVS特性进行实验验证,研究结果表明:相较于传统的ZVS条件,该方案能更精确地预判拓扑在各主要模态时能否达成ZVS,从而有利于谐振腔参数设计,证明了该方案的可行性和有效性。 展开更多
关键词 半桥LLC 零电压开通 多模态
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