背景与目的非小细胞肺癌的免疫治疗药物pembrolizumab需要基于包括免疫组织化学(immunohistochemistry,IHC)在内的检测结果,即通过检测程序性死亡配体1(programmed cell death protein 1,PD-L1)的表达等手段来预测治疗反应。评估肺腺癌...背景与目的非小细胞肺癌的免疫治疗药物pembrolizumab需要基于包括免疫组织化学(immunohistochemistry,IHC)在内的检测结果,即通过检测程序性死亡配体1(programmed cell death protein 1,PD-L1)的表达等手段来预测治疗反应。评估肺腺癌细胞学标本免疫细胞化学(immunocytochemistry,ICC)方法检测PD-L1的可行性,并探讨PD-L1表达与临床病理及分子特征的相关性。方法收集60例肺腺癌胸水细胞学标本应用PD-L1 sp263试剂按照生产说明书进行免疫细胞化学染色,同时对胸水细胞学标本做高通量二代测序(next-generation sequencing,NGS),探讨PD-L1与驱动基因突变的相关性。结果60例肺腺癌胸水细胞块标本ICC检测中,有35例PD-L1表达阳性,阳性表达率为58.3%。本院57例组织学标本PD-L1 IHC表达的阳性率为33.3%,细胞学标本与组织学标本差异无统计学意义(P>0.05)。60例细胞学标本中26例接受NGS检测,15例(57.7%)发现表皮生长因子受体(epidermal growth factorreceptor,EGFR)突变,经统计PD-L1表达与EGFR突变未发现相关性。PD-L1的阳性表达率与研究人群的年龄、性别、是否淋巴结或远处转移及是否进行放化疗或靶向治疗均未发现相关性(P>0.05)。结论在无手术标本可取时,胸水细胞学细胞块标本可以对PD-L1进行免疫细胞化学检测,其结果具有可行性。展开更多
A series of highly dispersed platinum‐deposited porous g‐C3N4 (Pt/pg‐C3N4) were successfully fabricated by a simple in situ photoreduction strategy using chloroplatinic acid and porous g‐C3N4 as precursors. Porou...A series of highly dispersed platinum‐deposited porous g‐C3N4 (Pt/pg‐C3N4) were successfully fabricated by a simple in situ photoreduction strategy using chloroplatinic acid and porous g‐C3N4 as precursors. Porous g‐C3N4 was fabricated by a pretreatment strategy using melamine as a raw material.The morphology, porosity, phase, chemical structure, and optical and electronic properties ofas‐prepared Pt/pg‐C3N4 were characterized. The photocatalytic activity of as‐prepared Pt/pg‐C3N4was preliminarily evaluated by the degradation of aqueous azo dyes methyl orange under visible light irradiation. The as‐prepared Pt/pg‐C3N4 were further applied to the degradation and mineralization of aqueous 4‐fluorophenol. The recyclability of Pt/pg‐C3N4 was evaluated under four consecutive photocatalytic runs.展开更多
Multidrug resistance(MDR), deined as the resistance of cancer cells to compounds with diverse structures and mechanisms of actions, signiicantly limits the eicacy of antitumor drugs. A major mechanism that mediates MD...Multidrug resistance(MDR), deined as the resistance of cancer cells to compounds with diverse structures and mechanisms of actions, signiicantly limits the eicacy of antitumor drugs. A major mechanism that mediates MDR in cancer is the overexpression of adenosine triphosphate(ATP)?binding cassette transporters. These transporters bind to their respective substrates and catalyze their elux from cancer cells, thereby lowering the intracellular concentra?tions of the substrates and thus attenuating or even abolishing their eicacy. In addition, cancer cells can become resistant to drugs via mechanisms that attenuate apoptosis and cell cycle arrest such as alterations in the p53, check point kinase, nuclear factor kappa B, and the p38 mitogen?activated protein kinase pathway. In this review, we discuss the mechanisms by which β?elemene, a compound extracted from Rhizoma zedoariae that has clinical antitumor eicacy, overcomes drug resistance in cancer.展开更多
The photocatalytic reduction of aqueous Cr(VI)to Cr(III)was preliminarily studied using porousg‐C3N4as a photocatalyst under acidic conditions.The observed synergistic photocatalytic effect ofporous g‐C3N4on a Cr(VI...The photocatalytic reduction of aqueous Cr(VI)to Cr(III)was preliminarily studied using porousg‐C3N4as a photocatalyst under acidic conditions.The observed synergistic photocatalytic effect ofporous g‐C3N4on a Cr(VI)/4‐chlorophenol(4‐CP)composite pollution system was further studiedunder different pH conditions.Compared with single‐component photocatalytic systems for Cr(VI)reduction or4‐CP degradation,the Cr(VI)reduction efficiency and4‐CP degradation efficiency weresimultaneously improved in the Cr(VI)/4‐CP composite pollution system.The synergistic photocatalyticeffect in the Cr(VI)/4‐CP composite pollution system can be attributed to the acceleratedredox reaction between dichromate and4‐CP by electron transfer with porous g‐C3N4.展开更多
In this paper,sulfonic groups functionalized annealed bio-based carbon microspheres loaded polytetrafluoroethylene(A-BCMSs-SO_(3)H@PTFE)fibers with high activity,high stability,and easy regeneration were successfully ...In this paper,sulfonic groups functionalized annealed bio-based carbon microspheres loaded polytetrafluoroethylene(A-BCMSs-SO_(3)H@PTFE)fibers with high activity,high stability,and easy regeneration were successfully fabricated by a simple method using low-cost raw materials.The characterization results showed that the annealed biomass carbon microspheres derived from waste Camellia oleifera shells were evenly distributed on the polytetrafluoroethylene fibers and the sulfonic groups can be successfully loaded on the surface of annealed biomass carbon microspheres by room temperature sulfonation.Subsequently,the as-prepared A-BCMSs-SO_(3)H@PTFE fibers were applied to the acidcatalyzed synthesis of liquid biofuel 5-ethoxymethylfurfural.The catalytic experiment results indicated that the annealing temperature and time during catalyst preparation have a significant effect on the activity and selectivity of A-BCMSs-SO_(3)H@PTFE fibers.The results of catalytic reaction kinetics showed that the yield of 5-ethoxymethylfurfural can reach more than 60%after 72 h of acid-catalyzed reaction.The stability test showed that the as-prepared A-BCMSs-SO_(3)H@PTFE fibers still maintained a stable acid catalytic activity after four recycles.展开更多
A series of biobased heterogeneous solid Br?nsted acid catalysts with perfect spherical microstructures are successfully fabricated directly from waste Camellia oleifera shells by a simple hydrothermal carbonization-a...A series of biobased heterogeneous solid Br?nsted acid catalysts with perfect spherical microstructures are successfully fabricated directly from waste Camellia oleifera shells by a simple hydrothermal carbonization-annealing-sulfonation process. 350℃ low temperature annealing process helps to increase the activity of the catalyst due to the simultaneous maintenance of the spherical microstructure and aromatic carbon framework. As a renewable catalyst with low cost, the as-prepared materials are successfully applied on the synthesis of green renewable liquid biofuel 5-ethoxymethylfurfural(EMF) directly from fructose. In the catalytic test, the influences of reaction time and temperature, fructose concentration, and adding amount of the catalyst on the yield of EMF are investigated systematically. As a result, the optimal reaction temperature is 100℃, the EMF yield monotonically increases with prolonging the reaction time from 3 to 24 h, the optimal fructose concentration is0.5 mmol, and the EMF yield gradually increases with increasing the adding amount of the catalyst from 50 to 150 mg. In addition, the asprepared catalysts exhibit considerably high stability in the current EMF synthesis system, and they can maintain a similar level of reactivity after four catalytic cycles.展开更多
In order to study the influence mechanism of structural type and size on the hydrodynamic performance of vertical curved V-type otter board,this paper based on the computational fluid dynamics method,a three-dimension...In order to study the influence mechanism of structural type and size on the hydrodynamic performance of vertical curved V-type otter board,this paper based on the computational fluid dynamics method,a three-dimensional numerical model of vertical curved V-type otter board is established to calculate and analyze its hydrodynamic performance,and the validity of the numerical simulation results is verified by designing and constructing a scaled model for dynamic flume experiments.On this basis,the effects of different structural parameters,such as the deflector angle,camber,and spacing,on the hydrodynamic performance of the vertical curved V-Type otter board are investigated and the optimal structural design scheme is determined.The results demonstrate that single or synchronous changes of the angle,camber,and spacing of the two deflectors have varying degrees of impact on the hydrodynamic performance of the vertical curved V-Type otter board.To improve the expansion effect of the otter board,setting the angle of the deflector A to 40◦and that of the deflector B to 30◦,or setting the camber of deflectors A and B to 18%,or setting the spacing between deflectors A and B to 380 mm and that between the deflector and the main panel to 670 mm can increase the lift coefficient of the vertical curved V-Type otter board to its maximum value.Considering the overall performance of the otter board,when the angle of the deflector A is set to 25◦and that of the deflector B is set to 30◦,or the camber of the deflector A is set to 6% and that of the deflector B is set to 9%,or the spacing between deflectors A and B is set to 340 mm and that between the deflector and the main panel is set to 610 mm,the lift-to-drag ratio of the otter board can be increased to its maximum value.展开更多
Photocatalytic selective transform native lignin into valuable chemicals is an attractive but challenging task.Herein,we report a mesoporous sulfur-doped carbon nitride(MSCN-0.5)which is prepared by a facile one-step ...Photocatalytic selective transform native lignin into valuable chemicals is an attractive but challenging task.Herein,we report a mesoporous sulfur-doped carbon nitride(MSCN-0.5)which is prepared by a facile one-step thermal condensation strategy.It is highly active and selective for the cleavage Cα-Cβbond inβ-O-4 lignin model compound under visible light radiation at room temperature,achieving 99%substrate conversion and 98%Cα-Cβbond cleavage selectivity.Mechanistic studies revealed that the Cβ-H bond of lignin model compounds activated by holes and generate key Cβradical intermediates,further induced the Cα-Cβbond cleavage by superoxide anion radicals(·O2-)to produce aromatic oxygenates.Waste Camellia oleifera shell(WCOS)was taken as a representative to further understand the reaction mechanisms on native lignin.33.2 mg of monophenolic compounds(Vanillin accounted for 22%and Syringaldehyde for 34%)can be obtained by each gram of WCOS lignin,which is 2.5 times as that of the pristine carbon nitride.The present work offers useful guidance for designing metal-free heterogeneous photocatalysts for Cα-Cβbond cleavage to harvest monophenolic compounds.展开更多
Sorption and cosorption of the nonionic herbicide mefenacet and two typical metals （copper and silver） on black soil and its components （kaolinite and humic acid） were investigated. It was found that because of th...Sorption and cosorption of the nonionic herbicide mefenacet and two typical metals （copper and silver） on black soil and its components （kaolinite and humic acid） were investigated. It was found that because of their different valences and properties, Cu2＋and Ag＋ presented different effects on the sorption of mefenacet. Due to the competition of Cu2＋, along with the shells of dense water formed by its surface complexation, for sorption surface area with mefenacet, the addition of Cu2＋ decreased the sorption amount of mefenacet on soil and its components, especially on humic acid. However, the addition of Ag＋ significantly enhanced the sorption of mefenacet, which was attributed to the softness of the cation that weakened the hydrophilicity of the local region around Ag＋-complexed functionalities, and thus mitigated the competitive sorption of water. In addition, the sorption of mefenacet on soil with or without the two metals was generally decreased with increasing pH, which was caused by the hydrolysis of carbonyl and carboxyl groups on the surface of the sorbents, π-π interaction between mefenacet and the soil organic matter, and so on. On the other hand, the presence of mefenacet seemed to have little effect on the sorption of Cu2＋ and Ag＋, indicating that Cu2＋ and Ag＋ might be sorbed strongly on the tested sorbents and the mefenacet added was too low in concentration to affect the sorption of the metals.展开更多
The recurrence and metastasis of children with mediastinal neuroblastoma(NB)are also occurred after surgery,chemotherapy,or radiotherapy.Strategies targeting the tumor microenvironment have been reported to improve su...The recurrence and metastasis of children with mediastinal neuroblastoma(NB)are also occurred after surgery,chemotherapy,or radiotherapy.Strategies targeting the tumor microenvironment have been reported to improve survival;however,thorough investigations of monocytes and tumor-associated macrophages(Mϕs)with specialized functions in NB are still lacking.Our data first demonstrated polypyrimidine tract binding protein 2(PTBP2)as a possible identifier in patients with mediastinal NB screened by proteomic profiling and that PTBP2 predicted good outcomes.Functional studies revealed that PTBP2 in NB cells induced the chemotactic activity and repolarization of tumor-associated monocytes and Mϕs,which,in turn,inhibited NB growth and dissemination.Mechanistically,PTBP2 prevents interferon regulatory factor 9 alternative splicing and upregulates signal transducers and activators of transcription 1 to stimulate C-C motif chemokine ligand 5(CCL5)and interferon-stimulated gene factor-dependent type I interferon secretion,to induce monocyte/Mϕs chemotaxis,and to sustain monocytes in a proinflammatory phenotype.Our study defined a critical event of PTBP2-induced monocytes/Mϕs in NB progression and revealed that RNA splicing occurred by PTBP2 benefits immune compartmentalization between NB cells and monocytes.This work revealed the pathological and biological role of PTBP2 in NB development and indicates that PTBP2-induced RNA splicing benefits immune compartmentalization and predicted a favorable prognosis in mediastinal NB.展开更多
文摘背景与目的非小细胞肺癌的免疫治疗药物pembrolizumab需要基于包括免疫组织化学(immunohistochemistry,IHC)在内的检测结果,即通过检测程序性死亡配体1(programmed cell death protein 1,PD-L1)的表达等手段来预测治疗反应。评估肺腺癌细胞学标本免疫细胞化学(immunocytochemistry,ICC)方法检测PD-L1的可行性,并探讨PD-L1表达与临床病理及分子特征的相关性。方法收集60例肺腺癌胸水细胞学标本应用PD-L1 sp263试剂按照生产说明书进行免疫细胞化学染色,同时对胸水细胞学标本做高通量二代测序(next-generation sequencing,NGS),探讨PD-L1与驱动基因突变的相关性。结果60例肺腺癌胸水细胞块标本ICC检测中,有35例PD-L1表达阳性,阳性表达率为58.3%。本院57例组织学标本PD-L1 IHC表达的阳性率为33.3%,细胞学标本与组织学标本差异无统计学意义(P>0.05)。60例细胞学标本中26例接受NGS检测,15例(57.7%)发现表皮生长因子受体(epidermal growth factorreceptor,EGFR)突变,经统计PD-L1表达与EGFR突变未发现相关性。PD-L1的阳性表达率与研究人群的年龄、性别、是否淋巴结或远处转移及是否进行放化疗或靶向治疗均未发现相关性(P>0.05)。结论在无手术标本可取时,胸水细胞学细胞块标本可以对PD-L1进行免疫细胞化学检测,其结果具有可行性。
基金supported by the National Natural Science Foundation of China (51568049, 51208248, 51468043, 21366024)the National Science Fund for Excellent Young Scholars (51422807)+1 种基金the Natural Science Foundation of Jiangxi Province, China (20161BAB206118, 20114BAB213015)the Natural Science Foundation of Jiangxi Provincial Department of Education, China (GJJ14515, GJJ12456)~~
文摘A series of highly dispersed platinum‐deposited porous g‐C3N4 (Pt/pg‐C3N4) were successfully fabricated by a simple in situ photoreduction strategy using chloroplatinic acid and porous g‐C3N4 as precursors. Porous g‐C3N4 was fabricated by a pretreatment strategy using melamine as a raw material.The morphology, porosity, phase, chemical structure, and optical and electronic properties ofas‐prepared Pt/pg‐C3N4 were characterized. The photocatalytic activity of as‐prepared Pt/pg‐C3N4was preliminarily evaluated by the degradation of aqueous azo dyes methyl orange under visible light irradiation. The as‐prepared Pt/pg‐C3N4 were further applied to the degradation and mineralization of aqueous 4‐fluorophenol. The recyclability of Pt/pg‐C3N4 was evaluated under four consecutive photocatalytic runs.
基金supported by St.John’s University Research Seed Grant (No. 579-1110-7002) to Zhe-Sheng Chen
文摘Multidrug resistance(MDR), deined as the resistance of cancer cells to compounds with diverse structures and mechanisms of actions, signiicantly limits the eicacy of antitumor drugs. A major mechanism that mediates MDR in cancer is the overexpression of adenosine triphosphate(ATP)?binding cassette transporters. These transporters bind to their respective substrates and catalyze their elux from cancer cells, thereby lowering the intracellular concentra?tions of the substrates and thus attenuating or even abolishing their eicacy. In addition, cancer cells can become resistant to drugs via mechanisms that attenuate apoptosis and cell cycle arrest such as alterations in the p53, check point kinase, nuclear factor kappa B, and the p38 mitogen?activated protein kinase pathway. In this review, we discuss the mechanisms by which β?elemene, a compound extracted from Rhizoma zedoariae that has clinical antitumor eicacy, overcomes drug resistance in cancer.
基金supported by the National Natural Science Foundation of China(51568049,51468043,21366024,21665018)the National Science Fund for Excellent Young Scholars(51422807)+2 种基金the Natural Science Foundation of Jiangxi Province,China(20161BAB206118,20171ACB21035)the Distinguished Youth Science Fund of Jiangxi Province(20162BCB23043)the Natural Science Foundation of Jiangxi Provincial Department of Education,China(GJJ14515)~~
文摘The photocatalytic reduction of aqueous Cr(VI)to Cr(III)was preliminarily studied using porousg‐C3N4as a photocatalyst under acidic conditions.The observed synergistic photocatalytic effect ofporous g‐C3N4on a Cr(VI)/4‐chlorophenol(4‐CP)composite pollution system was further studiedunder different pH conditions.Compared with single‐component photocatalytic systems for Cr(VI)reduction or4‐CP degradation,the Cr(VI)reduction efficiency and4‐CP degradation efficiency weresimultaneously improved in the Cr(VI)/4‐CP composite pollution system.The synergistic photocatalyticeffect in the Cr(VI)/4‐CP composite pollution system can be attributed to the acceleratedredox reaction between dichromate and4‐CP by electron transfer with porous g‐C3N4.
基金financially supported by the National Natural Science Foundation of China(21966023,21665018)the Natural Science Foundation of Jiangxi Province,China(20171ACB21035)
文摘In this paper,sulfonic groups functionalized annealed bio-based carbon microspheres loaded polytetrafluoroethylene(A-BCMSs-SO_(3)H@PTFE)fibers with high activity,high stability,and easy regeneration were successfully fabricated by a simple method using low-cost raw materials.The characterization results showed that the annealed biomass carbon microspheres derived from waste Camellia oleifera shells were evenly distributed on the polytetrafluoroethylene fibers and the sulfonic groups can be successfully loaded on the surface of annealed biomass carbon microspheres by room temperature sulfonation.Subsequently,the as-prepared A-BCMSs-SO_(3)H@PTFE fibers were applied to the acidcatalyzed synthesis of liquid biofuel 5-ethoxymethylfurfural.The catalytic experiment results indicated that the annealing temperature and time during catalyst preparation have a significant effect on the activity and selectivity of A-BCMSs-SO_(3)H@PTFE fibers.The results of catalytic reaction kinetics showed that the yield of 5-ethoxymethylfurfural can reach more than 60%after 72 h of acid-catalyzed reaction.The stability test showed that the as-prepared A-BCMSs-SO_(3)H@PTFE fibers still maintained a stable acid catalytic activity after four recycles.
基金supported by the National Natural Science Foundation of China (51568049, 21665018, 51468043, 21366024)the Natural Science Foundation of Jiangxi Province, China (20161BAB206118, 20171ACB21035)+1 种基金Distinguished Youth Science Fund of Jiangxi Province, China (20162BCB23043)the Natural Science Foundation of Jiangxi Provincial Department of Education, China (GJJ14515)
文摘A series of biobased heterogeneous solid Br?nsted acid catalysts with perfect spherical microstructures are successfully fabricated directly from waste Camellia oleifera shells by a simple hydrothermal carbonization-annealing-sulfonation process. 350℃ low temperature annealing process helps to increase the activity of the catalyst due to the simultaneous maintenance of the spherical microstructure and aromatic carbon framework. As a renewable catalyst with low cost, the as-prepared materials are successfully applied on the synthesis of green renewable liquid biofuel 5-ethoxymethylfurfural(EMF) directly from fructose. In the catalytic test, the influences of reaction time and temperature, fructose concentration, and adding amount of the catalyst on the yield of EMF are investigated systematically. As a result, the optimal reaction temperature is 100℃, the EMF yield monotonically increases with prolonging the reaction time from 3 to 24 h, the optimal fructose concentration is0.5 mmol, and the EMF yield gradually increases with increasing the adding amount of the catalyst from 50 to 150 mg. In addition, the asprepared catalysts exhibit considerably high stability in the current EMF synthesis system, and they can maintain a similar level of reactivity after four catalytic cycles.
基金supported by the National Natural Science Foundation of China(Grant No.31972845)the Open Fund of National Ocean Fisheries Engineering and Technology Research Center(Grant No.A1-2006-21-200208).
文摘In order to study the influence mechanism of structural type and size on the hydrodynamic performance of vertical curved V-type otter board,this paper based on the computational fluid dynamics method,a three-dimensional numerical model of vertical curved V-type otter board is established to calculate and analyze its hydrodynamic performance,and the validity of the numerical simulation results is verified by designing and constructing a scaled model for dynamic flume experiments.On this basis,the effects of different structural parameters,such as the deflector angle,camber,and spacing,on the hydrodynamic performance of the vertical curved V-Type otter board are investigated and the optimal structural design scheme is determined.The results demonstrate that single or synchronous changes of the angle,camber,and spacing of the two deflectors have varying degrees of impact on the hydrodynamic performance of the vertical curved V-Type otter board.To improve the expansion effect of the otter board,setting the angle of the deflector A to 40◦and that of the deflector B to 30◦,or setting the camber of deflectors A and B to 18%,or setting the spacing between deflectors A and B to 380 mm and that between the deflector and the main panel to 670 mm can increase the lift coefficient of the vertical curved V-Type otter board to its maximum value.Considering the overall performance of the otter board,when the angle of the deflector A is set to 25◦and that of the deflector B is set to 30◦,or the camber of the deflector A is set to 6% and that of the deflector B is set to 9%,or the spacing between deflectors A and B is set to 340 mm and that between the deflector and the main panel is set to 610 mm,the lift-to-drag ratio of the otter board can be increased to its maximum value.
基金finacially supported by the National Natural Science Foundation of China(Nos.21966023,21665018 and 51568049)the Natural Science Foundation of Jiangxi Province,China(No.20171ACB21035)Jiangxi Province Graduate Student Innovation Special Fund(No.YC2020-S513)。
文摘Photocatalytic selective transform native lignin into valuable chemicals is an attractive but challenging task.Herein,we report a mesoporous sulfur-doped carbon nitride(MSCN-0.5)which is prepared by a facile one-step thermal condensation strategy.It is highly active and selective for the cleavage Cα-Cβbond inβ-O-4 lignin model compound under visible light radiation at room temperature,achieving 99%substrate conversion and 98%Cα-Cβbond cleavage selectivity.Mechanistic studies revealed that the Cβ-H bond of lignin model compounds activated by holes and generate key Cβradical intermediates,further induced the Cα-Cβbond cleavage by superoxide anion radicals(·O2-)to produce aromatic oxygenates.Waste Camellia oleifera shell(WCOS)was taken as a representative to further understand the reaction mechanisms on native lignin.33.2 mg of monophenolic compounds(Vanillin accounted for 22%and Syringaldehyde for 34%)can be obtained by each gram of WCOS lignin,which is 2.5 times as that of the pristine carbon nitride.The present work offers useful guidance for designing metal-free heterogeneous photocatalysts for Cα-Cβbond cleavage to harvest monophenolic compounds.
基金supported by the Natural Science Foundation of Jiangsu Province (No. BK2009254)
文摘Sorption and cosorption of the nonionic herbicide mefenacet and two typical metals （copper and silver） on black soil and its components （kaolinite and humic acid） were investigated. It was found that because of their different valences and properties, Cu2＋and Ag＋ presented different effects on the sorption of mefenacet. Due to the competition of Cu2＋, along with the shells of dense water formed by its surface complexation, for sorption surface area with mefenacet, the addition of Cu2＋ decreased the sorption amount of mefenacet on soil and its components, especially on humic acid. However, the addition of Ag＋ significantly enhanced the sorption of mefenacet, which was attributed to the softness of the cation that weakened the hydrophilicity of the local region around Ag＋-complexed functionalities, and thus mitigated the competitive sorption of water. In addition, the sorption of mefenacet on soil with or without the two metals was generally decreased with increasing pH, which was caused by the hydrolysis of carbonyl and carboxyl groups on the surface of the sorbents, π-π interaction between mefenacet and the soil organic matter, and so on. On the other hand, the presence of mefenacet seemed to have little effect on the sorption of Cu2＋ and Ag＋, indicating that Cu2＋ and Ag＋ might be sorbed strongly on the tested sorbents and the mefenacet added was too low in concentration to affect the sorption of the metals.
基金from National Natural Science Foundation of China(82002635,82002636,and 82173593)Guangzhou Science and Technology Project(202102021227 and 202102020421)Shenzhen Municipal Commission of Science and Technology Innovation(JCYJ20220531093213030)。
文摘The recurrence and metastasis of children with mediastinal neuroblastoma(NB)are also occurred after surgery,chemotherapy,or radiotherapy.Strategies targeting the tumor microenvironment have been reported to improve survival;however,thorough investigations of monocytes and tumor-associated macrophages(Mϕs)with specialized functions in NB are still lacking.Our data first demonstrated polypyrimidine tract binding protein 2(PTBP2)as a possible identifier in patients with mediastinal NB screened by proteomic profiling and that PTBP2 predicted good outcomes.Functional studies revealed that PTBP2 in NB cells induced the chemotactic activity and repolarization of tumor-associated monocytes and Mϕs,which,in turn,inhibited NB growth and dissemination.Mechanistically,PTBP2 prevents interferon regulatory factor 9 alternative splicing and upregulates signal transducers and activators of transcription 1 to stimulate C-C motif chemokine ligand 5(CCL5)and interferon-stimulated gene factor-dependent type I interferon secretion,to induce monocyte/Mϕs chemotaxis,and to sustain monocytes in a proinflammatory phenotype.Our study defined a critical event of PTBP2-induced monocytes/Mϕs in NB progression and revealed that RNA splicing occurred by PTBP2 benefits immune compartmentalization between NB cells and monocytes.This work revealed the pathological and biological role of PTBP2 in NB development and indicates that PTBP2-induced RNA splicing benefits immune compartmentalization and predicted a favorable prognosis in mediastinal NB.
