AIM:To improve an asialoglycoprotein receptor(ASGPR)-based enrichment method for detection of circulating tumor cells(CTCs)of hepatocellular carcinoma(HCC).METHODS:Peripheral blood samples were collected from healthy ...AIM:To improve an asialoglycoprotein receptor(ASGPR)-based enrichment method for detection of circulating tumor cells(CTCs)of hepatocellular carcinoma(HCC).METHODS:Peripheral blood samples were collected from healthy subjects,patients with HCC or various other cancers,and patients with hepatic lesions or hepatitis.CTCs were enriched from whole blood by extracting CD45-expressing leukocytes with monoclonal antibody coated-beads following density gradient centrifugation.The remaining cells were cytocentrifuged on polylysine-coated slides.Isolated cells were treated by triple immunofluorescence staining with CD45antibody and a combination of antibodies against ASGPR and carbamoyl phosphate synthetase 1(CPS1),used as liver-specific markers,and costained with DAPI.The cell slide was imaged and stained tumor cells that met preset criteria were counted.Recovery,sensitivity and specificity of the detection methods were determined and compared by spiking experiments with various types of cultured human tumor cell lines.Expression of ASGPR and CPS1 in cultured tumor cells and tumor tissue specimens was analyzed by flow cytometry and triple immunofluorescence staining,respectively.RESULTS:CD45 depletion of leukocytes resulted in a significantly greater recovery of multiple amounts of spiked HCC cells than the ASGPR+selection(P s<0.05).The expression rates of either ASGPR or CPS1were different in various liver cancer cell lines,ranging between 18%and 99%for ASGPR and between 9%and 98%for CPS1.In both human HCC tissues and liver cancer cell lines,there were a few HCC cells that did not stain positive for ASGPR or CPS1.The mixture of monoclonal antibodies against ASGPR and CPS1identified more HCC cells than either antibody alone.However,these antibodies did not detect any tumor cells in blood samples spiked with the human breastcancer cell line MCF-7 and the human renal cancer cell line A498.ASGPR+or/and CPS1+CTCs were detected in 29/32(91%)patients with HCC,but not in patients with any other kind of cancer or any of the other test subjects.Furthermore,the improved method detected a higher CTC count in all patients examined than did the previous method(P=0.001),and consistently achieved 12%-21%higher sensitivity of CTC detection in all seven HCC patients with more than 40 CTCs.CONCLUSION:Negative depletion enrichment combined with identification using a mixture of antibodies against ASGPR and CPS1 improves sensitivity and specificity for detecting circulating HCC cells.展开更多
Active and passive anti-Aβimmunotherapies have successfully been used for the prevention and treatment of Alzheimer’s disease animal models.However,clinical use of these immunotherapies is not effective,because the ...Active and passive anti-Aβimmunotherapies have successfully been used for the prevention and treatment of Alzheimer’s disease animal models.However,clinical use of these immunotherapies is not effective,because the vaccination is administered too late.At 1 month of age,100μL of Aβ3–10-KLH peptide(vaccine,2μg/μL)was subcutaneously injected into the neck of an amyloid precursor protein/presenilin-1/tau transgenic(3×Tg-AD)mouse model.Aβ3–10-KLH peptide was re-injected at 1.5,2.5,3.5,4.5,5.5,and 6.5 months of age.Serum levels of Aβantibody were detected by enzyme-linked immunosorbent assay,while spatial learning and memory ability were evaluated by Morris water maze.Immunohistochemistry was used to detect total tau with HT7 and phosphorylated tau with AT8(phosphorylation sites Ser202 and Thr205)and AT180(phosphorylation site Thr231)antibodies in the hippocampus.In addition,western blot analysis was used to quantify AT8 and AT180 expression in the hippocampus.The results showed that after vaccine injection,mice produced high levels of Aβantibody,cognitive function was significantly improved,and total tau and phosphorylated tau levels were significantly reduced.These findings suggest that early active immunization with Aβ3–10-KLH vaccine can greatly reduce tau phosphorylation,thereby mitigating the cognitive decline of 3×Tg-AD mice.This study was approved by the Animal Ethics Committee of China Medical University,China(approval No.103-316)on April 2,2016.展开更多
We study the effects of infrared radiation on a two-dimensional Bardeen-Cooper-Schrieffer superconductor coupled with a normal metal substrate through a tunneling barrier.The phase transition is analyzed by inspecting...We study the effects of infrared radiation on a two-dimensional Bardeen-Cooper-Schrieffer superconductor coupled with a normal metal substrate through a tunneling barrier.The phase transition is analyzed by inspecting the stability of the system against perturbations of pairing potentials.We find an oscillating gap phase with a frequency not directly related to the radiation frequency,but instead resulting from the asymmetry of electron density of states of the system as well as the tunneling amplitude.When such a superconductor is in contact with another superconductor,gives rise to an unusual alternating Josephson current.展开更多
In this work, a fluorescein-derived fluorescent probe for H2S based on the thiolysis of dinitrophenyl ether is reported. This probe exhibits turn-on fluorescence imaging of H2S in living cells and bulk solutions with ...In this work, a fluorescein-derived fluorescent probe for H2S based on the thiolysis of dinitrophenyl ether is reported. This probe exhibits turn-on fluorescence imaging of H2S in living cells and bulk solutions with excellent selectivity. The reaction mechanism was explained by means of absorption, fluorescence and HPLC-MS.展开更多
Dear Editor,The coronavirus disease 2019(COVID-19)has resulted in more than 66.5 million cases globally in more than 191 countries with overl,529,000 mortalities as of 6 December.Currently,the COVID-19 continued to sp...Dear Editor,The coronavirus disease 2019(COVID-19)has resulted in more than 66.5 million cases globally in more than 191 countries with overl,529,000 mortalities as of 6 December.Currently,the COVID-19 continued to spread around the world,while the duration of antibody lasts and antibody level remained poorly understood,which are primary for safe and effective antiviral treatments and vaccines in the future.In this study,we comprehensively characterized the longitudinal changes of antibodies in recovered patients from 5-7 to 34-42 weeks after symptom onset.展开更多
The excited-state intramolecular proton transfer molecules based on chalcone （E）-3-（4＇-dimethylami- nophenyl）-l-（4＇-fluoro-2＇-hydroxyphenyl）-2-propen-l-one （DMF-HPPO） have been synthesized under microwave i...The excited-state intramolecular proton transfer molecules based on chalcone （E）-3-（4＇-dimethylami- nophenyl）-l-（4＇-fluoro-2＇-hydroxyphenyl）-2-propen-l-one （DMF-HPPO） have been synthesized under microwave irradiation. One-dimensional （1D） microwires and 2D microdisks of DMF-HPPO have been selectively prepared by controlling the solution polarity. XRD results revealed the two microcrystals exhibit distinct diffraction patterns, which indicates that they have belonged to different crystalline nature. The microcrystals demonstrate shape-dependent amplified spontaneous emissions （ASE） that the emission of ID microwire is central around 618 nm and the 2D microdisk emits fluorescence at 650 nm. This result reveals the controlled synthesis of two microcrystals and their consequent multicolor amplified spontaneous emission, providing considerable promise for the development and application of new opto-electronic devices.展开更多
基金Supported by Grants from the China National Key Projects for Infectious Disease,No.2012ZX10002012-10the National Nature Science Foundation of China,No.81172207,No.81272669 and No.81301830
文摘AIM:To improve an asialoglycoprotein receptor(ASGPR)-based enrichment method for detection of circulating tumor cells(CTCs)of hepatocellular carcinoma(HCC).METHODS:Peripheral blood samples were collected from healthy subjects,patients with HCC or various other cancers,and patients with hepatic lesions or hepatitis.CTCs were enriched from whole blood by extracting CD45-expressing leukocytes with monoclonal antibody coated-beads following density gradient centrifugation.The remaining cells were cytocentrifuged on polylysine-coated slides.Isolated cells were treated by triple immunofluorescence staining with CD45antibody and a combination of antibodies against ASGPR and carbamoyl phosphate synthetase 1(CPS1),used as liver-specific markers,and costained with DAPI.The cell slide was imaged and stained tumor cells that met preset criteria were counted.Recovery,sensitivity and specificity of the detection methods were determined and compared by spiking experiments with various types of cultured human tumor cell lines.Expression of ASGPR and CPS1 in cultured tumor cells and tumor tissue specimens was analyzed by flow cytometry and triple immunofluorescence staining,respectively.RESULTS:CD45 depletion of leukocytes resulted in a significantly greater recovery of multiple amounts of spiked HCC cells than the ASGPR+selection(P s<0.05).The expression rates of either ASGPR or CPS1were different in various liver cancer cell lines,ranging between 18%and 99%for ASGPR and between 9%and 98%for CPS1.In both human HCC tissues and liver cancer cell lines,there were a few HCC cells that did not stain positive for ASGPR or CPS1.The mixture of monoclonal antibodies against ASGPR and CPS1identified more HCC cells than either antibody alone.However,these antibodies did not detect any tumor cells in blood samples spiked with the human breastcancer cell line MCF-7 and the human renal cancer cell line A498.ASGPR+or/and CPS1+CTCs were detected in 29/32(91%)patients with HCC,but not in patients with any other kind of cancer or any of the other test subjects.Furthermore,the improved method detected a higher CTC count in all patients examined than did the previous method(P=0.001),and consistently achieved 12%-21%higher sensitivity of CTC detection in all seven HCC patients with more than 40 CTCs.CONCLUSION:Negative depletion enrichment combined with identification using a mixture of antibodies against ASGPR and CPS1 improves sensitivity and specificity for detecting circulating HCC cells.
基金supported by the National Natural Science Foundation of China,No.81371227(to YPC)
文摘Active and passive anti-Aβimmunotherapies have successfully been used for the prevention and treatment of Alzheimer’s disease animal models.However,clinical use of these immunotherapies is not effective,because the vaccination is administered too late.At 1 month of age,100μL of Aβ3–10-KLH peptide(vaccine,2μg/μL)was subcutaneously injected into the neck of an amyloid precursor protein/presenilin-1/tau transgenic(3×Tg-AD)mouse model.Aβ3–10-KLH peptide was re-injected at 1.5,2.5,3.5,4.5,5.5,and 6.5 months of age.Serum levels of Aβantibody were detected by enzyme-linked immunosorbent assay,while spatial learning and memory ability were evaluated by Morris water maze.Immunohistochemistry was used to detect total tau with HT7 and phosphorylated tau with AT8(phosphorylation sites Ser202 and Thr205)and AT180(phosphorylation site Thr231)antibodies in the hippocampus.In addition,western blot analysis was used to quantify AT8 and AT180 expression in the hippocampus.The results showed that after vaccine injection,mice produced high levels of Aβantibody,cognitive function was significantly improved,and total tau and phosphorylated tau levels were significantly reduced.These findings suggest that early active immunization with Aβ3–10-KLH vaccine can greatly reduce tau phosphorylation,thereby mitigating the cognitive decline of 3×Tg-AD mice.This study was approved by the Animal Ethics Committee of China Medical University,China(approval No.103-316)on April 2,2016.
基金Supported by the National Basic Research Program of China under Grant No 2015CB921101the National Natural Science Foundation of China under Grant No 11325416
文摘We study the effects of infrared radiation on a two-dimensional Bardeen-Cooper-Schrieffer superconductor coupled with a normal metal substrate through a tunneling barrier.The phase transition is analyzed by inspecting the stability of the system against perturbations of pairing potentials.We find an oscillating gap phase with a frequency not directly related to the radiation frequency,but instead resulting from the asymmetry of electron density of states of the system as well as the tunneling amplitude.When such a superconductor is in contact with another superconductor,gives rise to an unusual alternating Josephson current.
基金the National Natural Science Foundation of China(No.21276251)Ministry of Human Resources and Social Security of PRC+1 种基金the 100 talents program funded by Chinese Academy of SciencesState Key Laboratory of Fine Chemicals of China(No.KF1105)
文摘In this work, a fluorescein-derived fluorescent probe for H2S based on the thiolysis of dinitrophenyl ether is reported. This probe exhibits turn-on fluorescence imaging of H2S in living cells and bulk solutions with excellent selectivity. The reaction mechanism was explained by means of absorption, fluorescence and HPLC-MS.
基金This work was supported by the National Key Research and Development Project of China(2020YFC0842200,2020YFA0707801)the Beijing Natural Science Foundation(No.7202196)+3 种基金Beijing Nova Program(No.xx2018052)Science and Technology Development Plan Project of Jilin Province(20200901007SF)Cheng-Feng Qin was supported by the National Science Fund for Distinguished Young Scholar(No.81925025)the Innovation Fund for Medical Sciences(No.2019RU040)from the Chinese Academy of Medical Sciences(CAMS).
文摘Dear Editor,The coronavirus disease 2019(COVID-19)has resulted in more than 66.5 million cases globally in more than 191 countries with overl,529,000 mortalities as of 6 December.Currently,the COVID-19 continued to spread around the world,while the duration of antibody lasts and antibody level remained poorly understood,which are primary for safe and effective antiviral treatments and vaccines in the future.In this study,we comprehensively characterized the longitudinal changes of antibodies in recovered patients from 5-7 to 34-42 weeks after symptom onset.
基金supported by National Natural Science Foundation of China(No. 21603008)Beijing Municipal Education Committee(No. SQKM201610012006)Research Project of Talent Introduction Foundation of Beijing Institute of Fashion Technology(No. 2015A-16)
文摘The excited-state intramolecular proton transfer molecules based on chalcone （E）-3-（4＇-dimethylami- nophenyl）-l-（4＇-fluoro-2＇-hydroxyphenyl）-2-propen-l-one （DMF-HPPO） have been synthesized under microwave irradiation. One-dimensional （1D） microwires and 2D microdisks of DMF-HPPO have been selectively prepared by controlling the solution polarity. XRD results revealed the two microcrystals exhibit distinct diffraction patterns, which indicates that they have belonged to different crystalline nature. The microcrystals demonstrate shape-dependent amplified spontaneous emissions （ASE） that the emission of ID microwire is central around 618 nm and the 2D microdisk emits fluorescence at 650 nm. This result reveals the controlled synthesis of two microcrystals and their consequent multicolor amplified spontaneous emission, providing considerable promise for the development and application of new opto-electronic devices.
