AIM:To study the inflammatory microenvironment and expression of chemokines in hepatocellular carcinoma(HCC) in nude mice.METHODS:CBRH-7919 HCC cells were injected into the subcutaneous region of nude mice.Beginning t...AIM:To study the inflammatory microenvironment and expression of chemokines in hepatocellular carcinoma(HCC) in nude mice.METHODS:CBRH-7919 HCC cells were injected into the subcutaneous region of nude mice.Beginning two weeks after the challenge,tumor growth was measured every week for six weeks.The stromal microenvironment and inflammatory cell infiltration was assessed by immunohistochemistry in paired tumor and adjacent peritumoral samples,and macrophage phenotype was assessed using double-stain immunohistochemistry incorporating expression of an intracellular enzyme.A chemokine PCR array,comprised of 98 genes,was used to screen differential gene expressions,which were validated by Western blotting.Additionally,expression of identified chemokines was knocked-down by RNA interference,and the effect on tumor growth was assessed.RESULTS:Inflammatory cell infiltrates are a key feature of adjacent peritumoral tissues with increased macrophage,neutrophil,and T cell(specifically helperand activated subsets)infiltration.Macrophages within adjacent peritumoral tissues express inducible nitric oxide synthase,suggestive of a proinflammatory phenotype.Fifty-one genes were identified in tumor tissues during the progression period,including 50that were overexpressed(including CXCL1,CXCL2 and CXCL3)and three that were underexpressed(CXCR1,Ifg and Actb).RNA interference of CXCL1 in the CBRH-7919 cells decreased the growth of tumors in nude mice and inhibited expression of CXCL2,CXCL3and interleukin-1βprotein.CONCLUSION:These findings suggest that CXCL1plays a critical role in tumor growth and may serve as a potential molecular target for use in HCC therapy.展开更多
Gaofang(medicated paste),also known as gaoji in Chinese,is one of the eight dosage forms of traditional Chinese medicine.Originated in the Han and Tang dynasties,it is widely used in Shanghai,Jiangsu,Zhejiang,and Guan...Gaofang(medicated paste),also known as gaoji in Chinese,is one of the eight dosage forms of traditional Chinese medicine.Originated in the Han and Tang dynasties,it is widely used in Shanghai,Jiangsu,Zhejiang,and Guangdong of China at present.It is generally believed that Gaofang can help reinforce deficiency,delay aging,and regulate the overall health status.Currently,Gaofang has been extensively used in the treatment of cardiovascular disease.Based on the relevant literature and clinical experience,this article reviews the general situation and the clinical application of Gaofang as well as the common Chinese medicines in Gaofang for cardiovascular diseases.展开更多
To investigate the protective effects and underlying mechanisms of Qin-Zhu-Liang-Xue decoction(QZLX)in atopic dermatitis(AD)and glucocorticoid resistance,we conducted a single-blinded,randomized controlled clinical tr...To investigate the protective effects and underlying mechanisms of Qin-Zhu-Liang-Xue decoction(QZLX)in atopic dermatitis(AD)and glucocorticoid resistance,we conducted a single-blinded,randomized controlled clinical trial to evaluate the efficacy and safety of this concoction.Network pharmacology analysis was performed and validated through clinical studies.The efficacy,safety,and mechanism of action of QZLX and glucocorticoid receptor(GR)αrecombinant protein were assessed in AD mice induced by 2,4-dinitrofluorobenzene(DNFB).Correlation analysis was performed to determine the clinical relevance of GRα.The trial demonstrated that patients who received QZLX showed considerable improvements in their Scoring Atopic Dermatitis(SCORAD)and Dermatology Life Quality Index(DLQI)scores compared with those who received mizolastine at week 4.Network pharmacological analysis identified GRαas a key target for QZLX in AD treatment.QZLX administration increased the serum GRαexpression in AD patients,alleviated AD symptoms in mice,decreased inflammatory cytokine expression,and increased GRαexpression without affecting liver or kidney function.In addition,GRαrecombinant protein improved AD-like skin lesions in DNFB-induced mice.A negative correlation was observed between GRαexpression and clinical parameters,including SCORAD,DLQI,and serum IgE levels.QZLX alleviates AD symptoms through the upregulation of GRαand thus presents a novel therapeutic strategy for the prevention of glucocorticoid resistance in AD management.展开更多
基金Supported by Natural Science Foundation of China,No.81072954
文摘AIM:To study the inflammatory microenvironment and expression of chemokines in hepatocellular carcinoma(HCC) in nude mice.METHODS:CBRH-7919 HCC cells were injected into the subcutaneous region of nude mice.Beginning two weeks after the challenge,tumor growth was measured every week for six weeks.The stromal microenvironment and inflammatory cell infiltration was assessed by immunohistochemistry in paired tumor and adjacent peritumoral samples,and macrophage phenotype was assessed using double-stain immunohistochemistry incorporating expression of an intracellular enzyme.A chemokine PCR array,comprised of 98 genes,was used to screen differential gene expressions,which were validated by Western blotting.Additionally,expression of identified chemokines was knocked-down by RNA interference,and the effect on tumor growth was assessed.RESULTS:Inflammatory cell infiltrates are a key feature of adjacent peritumoral tissues with increased macrophage,neutrophil,and T cell(specifically helperand activated subsets)infiltration.Macrophages within adjacent peritumoral tissues express inducible nitric oxide synthase,suggestive of a proinflammatory phenotype.Fifty-one genes were identified in tumor tissues during the progression period,including 50that were overexpressed(including CXCL1,CXCL2 and CXCL3)and three that were underexpressed(CXCR1,Ifg and Actb).RNA interference of CXCL1 in the CBRH-7919 cells decreased the growth of tumors in nude mice and inhibited expression of CXCL2,CXCL3and interleukin-1βprotein.CONCLUSION:These findings suggest that CXCL1plays a critical role in tumor growth and may serve as a potential molecular target for use in HCC therapy.
基金financed by the grants from Shanghai Traditional Chinese Medicine Expert Heritage Studio Construction Project(No.2020ZYGZS-002)Project of Traditional Chinese Medicine Processing and Inheritance Base of Shanghai Health and Wellness Committee(No.30304118220)Chinese Medicine Research Project of Shanghai Health and Wellness Committee(No.2020YP001).
文摘Gaofang(medicated paste),also known as gaoji in Chinese,is one of the eight dosage forms of traditional Chinese medicine.Originated in the Han and Tang dynasties,it is widely used in Shanghai,Jiangsu,Zhejiang,and Guangdong of China at present.It is generally believed that Gaofang can help reinforce deficiency,delay aging,and regulate the overall health status.Currently,Gaofang has been extensively used in the treatment of cardiovascular disease.Based on the relevant literature and clinical experience,this article reviews the general situation and the clinical application of Gaofang as well as the common Chinese medicines in Gaofang for cardiovascular diseases.
基金supported by the National Natural Science Foundation of China(No.82004359)Xinglin Youth Scholar of Shanghai University of Traditional Chinese Medicine(No.RY411.33.10)+10 种基金Youth Talent Promotion Project of China Association of Traditional Chinese Medicine(2021-2023)Category A(No.CACM-2021-QNRC2-A10)Health Young Talents of Shanghai Municipal Health Commission(No.2022YQ026)“Chen Guang”project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation(No.22CGA50)The Clinical Research Plan of SHDC(No.SHDC22022302)High-level Chinese Medicine Key Discipline Construction Project(Integrative Chinese and Western Medicine Clinic)of National Administration of TCM(No.zyyzdxk-2023065)Shanghai Dermatology Research Center(No.2023ZZ02017)Shanghai Dermatology Hospital Demonstration Research Ward Project(No.SHDC2023CRW009)Evidence-based Dermatology Base sponsored by State Administration of Traditional Chinese Medicine,Shanghai Municipal Project for the Construction of Traditional Chinese Medicine Expert Heritage Studio(No.2020ZYGZS-002)Clinical Transformation Incubation Program in Hospital(No.lczh2023-01)Professional Technical Service Platform Construction Project of Shanghai Science and Technology Commission(No.21DZ2294500)Shanghai key Discipline Construction Project of Traditional Chinese Medicine(No.shzyyzdxk-2024104).
文摘To investigate the protective effects and underlying mechanisms of Qin-Zhu-Liang-Xue decoction(QZLX)in atopic dermatitis(AD)and glucocorticoid resistance,we conducted a single-blinded,randomized controlled clinical trial to evaluate the efficacy and safety of this concoction.Network pharmacology analysis was performed and validated through clinical studies.The efficacy,safety,and mechanism of action of QZLX and glucocorticoid receptor(GR)αrecombinant protein were assessed in AD mice induced by 2,4-dinitrofluorobenzene(DNFB).Correlation analysis was performed to determine the clinical relevance of GRα.The trial demonstrated that patients who received QZLX showed considerable improvements in their Scoring Atopic Dermatitis(SCORAD)and Dermatology Life Quality Index(DLQI)scores compared with those who received mizolastine at week 4.Network pharmacological analysis identified GRαas a key target for QZLX in AD treatment.QZLX administration increased the serum GRαexpression in AD patients,alleviated AD symptoms in mice,decreased inflammatory cytokine expression,and increased GRαexpression without affecting liver or kidney function.In addition,GRαrecombinant protein improved AD-like skin lesions in DNFB-induced mice.A negative correlation was observed between GRαexpression and clinical parameters,including SCORAD,DLQI,and serum IgE levels.QZLX alleviates AD symptoms through the upregulation of GRαand thus presents a novel therapeutic strategy for the prevention of glucocorticoid resistance in AD management.
