In wastewater treatment systems,extracting meaningful features from process data is essential for effective monitoring and control.However,the multi-time scale data generated by different sampling frequencies pose a c...In wastewater treatment systems,extracting meaningful features from process data is essential for effective monitoring and control.However,the multi-time scale data generated by different sampling frequencies pose a challenge to accurately extract features.To solve this issue,a multi-timescale feature extraction method based on adaptive entropy is proposed.Firstly,the expert knowledge graph is constructed by analyzing the characteristics of wastewater components and water quality data,which can illustrate various water quality parameters and the network of relationships among them.Secondly,multiscale entropy analysis is used to investigate the inherent multi-timescale patterns of water quality data in depth,which enables us to minimize information loss while uniformly optimizing the timescale.Thirdly,we harness partial least squares for feature extraction,resulting in an enhanced representation of sample data and the iterative enhancement of our expert knowledge graph.The experimental results show that the multi-timescale feature extraction algorithm can enhance the representation of water quality data and improve monitoring capabilities.展开更多
Background:Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects.Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs(miRNAs)afte...Background:Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects.Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs(miRNAs)after exercise and found that some of them act as exerkines.However,whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains unknown.Methods:A 9-day treadmill training was used as an exercise model in C57BL/6 mice.Liver-specific adeno-associated virus 8 was used to knock down microRNA-122-5p(miR-122-5p).Human umbilical vein endothelial cells were used in vitro.Results:Among these differentially expressed extracellular vesicle miRNAs,miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in vitro.Exercise increased circulating levels of miR-122-5p,which was produced mainly by the liver and shuttled by extracellular vesicles in mice.Inhibition of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles,and exerciseimproved muscle performance in mice.Mechanistically,miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells,and miR-122-5p upregulated endothelial cell fatty-acid utilization by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase(AGPAT1).In addition,miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in mice.Conclusion:These findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p,which enhances fatty acid utilization by targeting AGPAT1 in endothelial cells,highlighting the therapeutic potential of miR-122-5p in tissue repair.展开更多
Although a few pristine metal-organic frameworks(MOFs) of graphene analogue topology exhibit high intrinsic electrical conductivity, their use in lithium-ion batteries(LIBs) is still hampered by unfavorable Li+adsorpt...Although a few pristine metal-organic frameworks(MOFs) of graphene analogue topology exhibit high intrinsic electrical conductivity, their use in lithium-ion batteries(LIBs) is still hampered by unfavorable Li+adsorption energy(ΔEa). In this paper, an electroconductive ferrocene-based MOF@MXene heterostructure is built to provide stable anodes for Li+storage. Charge density difference and planar average potential charge density show substantial redistribution of charges at the interfaces, transferring from MXene to MOF layers. Moreover, density functional theory(DFT) calculations reveal that the interaction between MXene and MOF significantly increases the ΔEa. As a result, the heterostructure anode exhibits high capacities and outstanding cycling stability with a capacity retention of 80% after 5000 cycles at 5 A g^(-1), outperforming mono-component MXene and MOF. Furthermore, the heterostructure anode is built into a full cell with a commercial NCM 532 cathode, delivering a high energy density of 611 Wh kg^(-1)and power density of 7600 W kg^(-1). The developed conductive MOF@MXene heterogeneity for improved LIB offers valuable insights into the design of advanced electrode materials for energy storage.展开更多
North China Mountain Walnut (NCMW) is one of the ancestors of extant cultivated species, and a valuable gene resource for resistance breeding of walnut in China. Inter-Simple Sequence Repeat (ISSR) primers were design...North China Mountain Walnut (NCMW) is one of the ancestors of extant cultivated species, and a valuable gene resource for resistance breeding of walnut in China. Inter-Simple Sequence Repeat (ISSR) primers were designed to evaluate the level and pattern of genetic diversity in eight populations of NCMW. Nine ISSR primers yielded 91 amplification products with different sizes, of which 84 (92.31%) were polymorphic. A high species-level genetic diversity was detected with Nei’s (H = 0.2592) and Shannon’s diversity (I = 0.4003). In contrast, the population-level genetic diversity was relatively lower (PPB = 43.27%, H = 0.1347, I = 0.1862). Coefficient of populations differentiation (GST) was 0.5066, indicating that inter-population and intra-population variation contributed 50.66% and 49.34% respectively to the total genetic variability. This relative level of variation was further supported by AMOVA analysis. Limited gene flow (Nm = 0.5133.), habitat fragmentation and geographical isolation might be responsible for the population structure of NCMW. UPGMA cluster analysis classified the eight populations into three groups which showed no significant relationship between the genetic similarity coefficient and geographic origin but showed remarkable association with morpho-physiological characters, particularly nut traits. The results of the study provide species-level and population-level genetic profiles for further exploitation and conservation of genetic diversity of NCMW.展开更多
Platelets aggregation and thrombosis formation are major reasons of cardiovascular and cerebral vascular diseases.To develop novel,effective and safe agents for inhibiting platelet aggregation and preventing above dis...Platelets aggregation and thrombosis formation are major reasons of cardiovascular and cerebral vascular diseases.To develop novel,effective and safe agents for inhibiting platelet aggregation and preventing above diseases is urgently needed.Some traditional Chinese medicines for“Houxue Huayu”have been shown to inhibit platelet aggregation potently.They are used frequently in China and also in Asian countries.Recently,the major effective components of Pueraria lobata,Salvia miltiorrhiza Bunge,Apium graveolens L.and so on were studied.The mechanisms and the molecular targets of puerarin,salvianolic acid B and the analogue of 3-n-butylphthalide,dl-PHPB were investigated.Four platelet aggregation inducers,ADP,arachidonic acid(AA),collagen(Col)and thrombin,were used in the study.展开更多
This paper presents a subspace identification method for closed-loop systems with unknown deterministic disturbances.To deal with the unknown deterministic disturbances,two strategies are implemented to construct the ...This paper presents a subspace identification method for closed-loop systems with unknown deterministic disturbances.To deal with the unknown deterministic disturbances,two strategies are implemented to construct the row space that can be used to approximately represent the unknown deterministic disturbances using the trigonometric functions or Bernstein polynomials depending on whether the disturbance frequencies are known.For closed-loop identification,CCF-N4SID is extended to the case with unknown deterministic disturbances using the oblique projection.In addition,a proper Bernstein polynomial order can be determined using the Akaike information criterion(AIC)or the Bayesian information criterion(BIC).Numerical simulation results demonstrate the effectiveness of the proposed identification method for both periodic and aperiodic deterministic disturbances.展开更多
Nordmann's Greenshank(Tringa guttifer)is a globally endangered species that has received little research attention.It is threatened by rapid habitat loss,an incomplete network of protected sites,and lack of long-t...Nordmann's Greenshank(Tringa guttifer)is a globally endangered species that has received little research attention.It is threatened by rapid habitat loss,an incomplete network of protected sites,and lack of long-term data on population dynamics.Citizen science data can be combined with survey data to support population estimation and conservation gap analysis.From 2020 to 2021,Nordmann's Greenshank was surveyed in Tiaozini,Xiaoyangkou,and Dongling on the southern coast of Jiangsu Province,China,and the global population of the species was re-evaluated using the data obtained.We integrated citizen science data from eBird and the China Bird Report from 2000 to 2020 with the survey results to identify important habitats harboring over 1%of its total population,and compared this data with existing protected areas to identify gaps in its global conservation.Our survey found that Tiaozini supported at least 1194 individuals.Consequently,its global population was reestimated to be 1500-2000.Moreover,45 important habitats were identified based on citizen data and survey results.Although 44.4%and 50.0%of the priority sites in the world and China,respectively,are located outside protected areas,the Conservation Effectiveness Index(C)is 68.4%and 71.1%,respectively,showing that the current coverage of protected areas for this part of its range is reasonable.This study presents the most complete and recent population data to date.Tiaozini is the most important migration stopover site for Nordmann's Greenshanks.The species is under threat in terms of breeding,wintering,and stopover sites.Therefore,we suggest improving monitoring,establishing new protected sites to complete the habitat protection network,and improving the effectiveness of existing habitat protection strategies,including further developing high tide roosting sites.展开更多
The yield and yield components of Japonica variety Tongjing 981 under different density and fertilization levels were studied through regression and correlation and path analysis. The results showed that the number of...The yield and yield components of Japonica variety Tongjing 981 under different density and fertilization levels were studied through regression and correlation and path analysis. The results showed that the number of panicles per unit area, number of filled grains per panicle and 1 000-grain weight all had very significant yield increasing effects, and the number of panicles per unit area played a leading role. However, the yield increasing effects of the number of panicles per unit area and number of filled grains per panicle are equally important when the basic seedlings are more or the N fertilizer application rate is large. In practical production, a major factor should be determined among the yield components, and rational cultivation measures should be taken accordingly, to improve yield.展开更多
To correct the light scattering property parameters of milk fat for improving the detection accuracy,the Mie-theory was used to establish a predictive model for light scattering properties of milk fat globule with mul...To correct the light scattering property parameters of milk fat for improving the detection accuracy,the Mie-theory was used to establish a predictive model for light scattering properties of milk fat globule with multi-diameter distributions, by means of Monte Carlo approach to simulate actual multi-diameter size distribution of milk fat globule in milk fat solution. Scattering coefficient and absorption coefficient of multidiameter distribution milk fat particles were calculated by simulating the particles size distribution in milk fat solution. And the light scattering properties of multi-diameter distribution was compared with that of volume mean diameter,Sauter mean diameter and numerical mean diameter in milk fat solution. Theoretical simulation results indicate that the scattering coefficient and absorption coefficient of milk fat particles are determined by the particle size distribution in milk fat solution. There is a distinct difference in scattering characteristics between the milk fat particles with multi-diameter distribution and that with mean diameters. Compared to that with multi-diameter distribution,the scattering coefficient of the milk fat particles with mean diameter has a maximum mean deviation of 9042 m-1. The particle size distribution is not completely determined by the mean diameters. The dependence of the light scattering properties on the particle size distribution should be considered into the model and simulation. Therefore,it is found that the particle size distribution in milk fat solution is an essential and critical factor to significantly improve the detection accuracy of milk fat content.展开更多
Objective: To construct the small interfering RNA (siRNA) expression vector of carcino-embryonic antigen (CEA) and inhibit the expression of CEA in EC9706 cells by RNA interference. Methods: Two pairs of oligonucleoti...Objective: To construct the small interfering RNA (siRNA) expression vector of carcino-embryonic antigen (CEA) and inhibit the expression of CEA in EC9706 cells by RNA interference. Methods: Two pairs of oligonucleotide sequences were designed and synthesized according to the encoding sequence of mRNA of CEA. The annealed oligonucleotide frag-ments were cloned into pRNAT-U6.2 expression vector and identified by sequencing. The recombinant plasmid pRNAT-U6.2-CEA was transfected into EC9706 cells. The expression of CEA in the stable transfected cells was assayed by real time PCR and Western blot. Results: DNA sequencing showed that the oligonucleotide fragments were correctly inserted into pRNAT-U6.2 vector, and CEA expression in the transfected cells was down-regulated significantly by pRNAT-U6.2-CEA at both the mRNA and protein levels. Conclusion: The siRNA expression vector of CEA is successfully constructed and inhibits CEA expression in EC9706 cells. This facilitates further studies of the function of CEA at the molecular level.展开更多
In this work,we proposed a ratiometric silver nanoclusters(AgNCs)fluorescent assay by designing a bifunctional-blockeraided hybridization chain reaction(HCR).Hairpin probe 1(HP1)containing two special DNA fragments(5...In this work,we proposed a ratiometric silver nanoclusters(AgNCs)fluorescent assay by designing a bifunctional-blockeraided hybridization chain reaction(HCR).Hairpin probe 1(HP1)containing two special DNA fragments(5′-CAC CGC T-3′and 5′-ATT TGC CTT TTG GGG ACG GATA-3′)at two terminals creates a red-emitting AgNC nucleation sequence(rNS,5′-CAC CGC TAT TTG CCT TTT GGG GAC GGATA-3′).We found that the presence of a toehold fragment(5′-TGCCC-3′)in HP1 could silence the rNS.Upon the addition of a target nucleic acid,HCR of HP1 and hairpin probe 2(HP2)could be initiated,resulting in the formation of long chain of DNA duplexes with multibranched rNS.As the toehold fragment in HP1participated in generating duplexes,a strong emission of rNS-templated AgNCs was observed at 670 nm.More significantly,a bifunctional blocker was introduced not only to reduce the background red-emitting fluorescence but also to play as an internal green-emitting AgNCs nucleation sequence.On the one hand,the blocker could increase the signal-to-noise-ratio of the constructed biosensor,and on the other hand,the blocker also helped to prepare ratiometric HCR-AgNCs assay with self-calibrating ability to strengthen its reproducibility.Compared with the traditional HCR-AgNCs sensors,the developed ratiometric assay based on the bifunctional-blocker-aided HCR has higher reliability,which is important for the fabrication of biosensors in various fields for practical biosensing applications.展开更多
Although immobilization-free and label-free electrochemical DNA(E-DNA)biosensors have engaged tremendous interest due to their superior properties,such as easy operation,time-saving and cost-saving,most of them are fa...Although immobilization-free and label-free electrochemical DNA(E-DNA)biosensors have engaged tremendous interest due to their superior properties,such as easy operation,time-saving and cost-saving,most of them are fabricated in homogeneous modes and usually produce high background current.In the present work,we proposed a new immobilization-free and label-free heterogeneous E-DNA assay based on a dual-blocker-aided multibranched hybridization chain reaction(HCR)for one-pot nucleic acid detection with zero background.The target nucleic acid triggers the HCR involving cascaded hybridization between two metastable hairpins,resulting in the generation of HCR products with multibranched arms,which can be captured onto the electrode viaπ-πstacking interactions between multibranched arms and reduced graphene oxide(rGO).Prior to the incubation process with an electrode,two blockers are designed to prohibit the nonspecific absorption of unreacted hairpin probes.Thus,an immobilization-free and label-free heterogeneous electrochemical assay for one-pot nucleic acid detection with zero background is readily realized.This strategy also presents additional merits of simplicity and cheap cost,since probe immobilization,signal tag labeling,and multiple incubation processes are avoided.Therefore,the as-proposed effective and versatile biosensor has great potential to be applied in nucleic acid-related practical biosensing.展开更多
Background and Aims:Metabolic dysfunction-associ-ated fatty liver disease(MAFLD)is a new concept,pro-posed in 2020;however,its applicability in Asia populations has yet to be evaluated.Therefore,we aimed to compare th...Background and Aims:Metabolic dysfunction-associ-ated fatty liver disease(MAFLD)is a new concept,pro-posed in 2020;however,its applicability in Asia populations has yet to be evaluated.Therefore,we aimed to compare the difference in epidemiological and clinical characteris-tics between MAFLD and non-alcoholic fatty liver disease(NAFLD)among Asian populations.Methods:Based on the Jinchang cohort,30,633 participants were collected.The prevalence and incidence of MAFLD and NAFLD were used to analyze the epidemic characteristics and its overlapping effects.In addition,the corresponding clinical character-istics of the two diagnostic criteria populations were com-pared.Results:The prevalence rates of MAFLD and NAFLD were 21.03%and 18.83%,respectively.After an average 2.28-year follow-up,the incidence densities of MAFLD and NAFLD were 41.58 per 1,000 person-years and 37.69 per 1,000 person-years,respectively.With the increase of baseline age,body mass index(BMI),and waist circumfer-ence(WC)levels,the prevalence and incidence of MAFLD and NAFLD were on the rise(all ptrend<0.05).Among the total patients diagnosed at baseline or follow-up,most pa-tients had both MAFLD and NAFLD,accounting for 78.84%and 82.88%,respectively.Compared with NAFLD,MAFLD patients had greater proportions of males and metabolic diseases(diabetes,dyslipidemia),and had higher BMI,WC,liver enzymes,blood glucose,and lipid levels in the base-line diagnosis patients(p<0.05).Additionally,lean MAFLD patients had higher metabolic disorders than lean NAFLD patients(p<0.05).Conclusions:Compared with NAFLD,the newly proposed definition of MAFLD is more practical and accurate,and it can help identify more fatty liver pa-tients with high-risk diseases.展开更多
A finite-difference-time-domain(FDTD)approach is undertaken to investigate the extraordinary optical transmission(EOT)phenomenon of Au circular aperture arrays deposited on a Bragg fiber facet for refractive index(RI)...A finite-difference-time-domain(FDTD)approach is undertaken to investigate the extraordinary optical transmission(EOT)phenomenon of Au circular aperture arrays deposited on a Bragg fiber facet for refractive index(RI)sensing.Investigation shows that the choice of effective indices and modal loss of the Bragg fiber core modes will affect the sensitivity enhancement by using a mode analysis approach.The critical parameters of Bragg fiber including the middle dielectric RI,as well as its gap between dielectric layers,which affect the EOT and RI sensitivity for the sensor,are discussed and optimized.It is demonstrated that a better sensitivity of 156±5 nm per refractive index unit(RIU)and an averaged figure of merit exceeding 3.5 RIU‒1 are achieved when RI is 1.5 and gap is 0.02μm in this structure.展开更多
Seeking continuous development,a modern community must also be able to adapt to future possible challenges using constrained or limited resources.As a revolutionary communication paradigm,the Internet of Things(IoT)em...Seeking continuous development,a modern community must also be able to adapt to future possible challenges using constrained or limited resources.As a revolutionary communication paradigm,the Internet of Things(IoT)empowers the cutting-edge and emerging applications which enable manifold new intelligent services towards a smart community.The sophisticated ecosystem of a digital community is made feasible by the IoT infrastructure,which also provides community control with access to a wealth of actual data.In addition,IoT platforms empower the ubiquitous computing ability,providing more potentials to the actuators in perception layer in the IoT architecture.With more and more population in the urban areas,sustainability issues have become a key factor to consider in the development of a digital community.We give a modern survey in this study on the most recent developments in IoT for sustainable digital communities.After carefully examining the most recent literature,we specifically highlight the various smart digital community application scenarios,such as smart buildings,energy management,green transportation,trash management,etc.We also look into a number of major issues facing the use of IoT technology in digital communities.Furthermore,we discuss potential future applications and future research areas for IoT,the critical component of sustainable digital communities.展开更多
Agarophyton vermiculophyllum is an agarophytic red alga originating from Northeast Asia that successfully spread to Europe and North America in the last 20 years.In this study,three hsp70 genes(hsp70-l,hsp70-2,and hsp...Agarophyton vermiculophyllum is an agarophytic red alga originating from Northeast Asia that successfully spread to Europe and North America in the last 20 years.In this study,three hsp70 genes(hsp70-l,hsp70-2,and hsp70-3)and one hsp90 gene were cloned,and real-time reverse transcription polymerase chain reaction(RT-qPCR)was used to measure their transcriptional levels in three kinds of thalli(tetrasporophytes,and male/female gametophytes)belonging to diploid or haploid phases in the life cycle of A.vermiculophyllum.The results show that the three Hsp70s in A.vermiculophyllum clustered into three different groups,and the locations of the putative Hsp70-l,Hsp70-2,and Hsp70-3 were in the cytoplasm,endoplasmic reticulum and chloroplast,respectively,according to their sequences.Beside on the same research,the putative Hsp90 was supposed to have a cytoplasmic location.The RT-qPCR results show that the three hsp70 genes were highly upregulated in gametophytes as compared to tetrasporophytes but the transcriptional level of hsp90 did not show such a significant increase.The chloroplast hsp70-3 exhibited the highest upregulation and the transcriptional level in creased more than 570 fold in female gametophytes,and 17 fold in male gametophytes,compared with tetrasporophytes.Therefore,cpHsp70-3 might act more like a chaperon molecule involved in haploid development under natural condition,while Hsp70-l and Hsp70-2 were more active in stress resistance.展开更多
Nobiletin(NOB) may have a potential effect on metabolic syndrome. In the present study, we aimed to explore the latent mechanisms of NOB for the treatment of metabolic syndrome based on network pharmacology and molecu...Nobiletin(NOB) may have a potential effect on metabolic syndrome. In the present study, we aimed to explore the latent mechanisms of NOB for the treatment of metabolic syndrome based on network pharmacology and molecular docking methods. The potential targets of NOB were retrieved and identified from six databases, such as the Traditional Chinese Medicine Systems Pharmacology Database. The metabolic syndrome-related targets were retrieved from six databases as follows: the Drug Bank database, Gene Cards database, the Online Mendelian Inheritance in Man database, Pharm GKB database, Therapeutic Target Database, and Dis Ge Net database. Gene ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis of 60 intersected genes were performed in R software(Bioconductor, cluster Profiler) to investigate the molecular mechanisms. Subsequently, the ingredient-target-pathway network of NOB was constructed and visualized through Cytoscape 3.8.0 software. Protein-protein interaction network was constructed to screen hub genes in the treatment of NOB on metabolic syndrome through The Search Tool for the Retrieval of Interacting Genes/Proteins and visualized by Cytoscape 3.8.0 software. Afterward, molecular docking was used to analyze the score of the hub genes with NOB. Cumulatively, 105 targets of NOB were identified. Moreover, 1975 metabolic syndrome-related genes were acquired from six databases after combining and deleting the repeated items, and the overlap of metabolic syndrome-related genes with NOB-related target genes identified 60 intersection genes of NOB against metabolic syndrome. Moreover, 1858 GO entries of NOB on metabolic syndrome were identified, and 153 pathways were screened based on GO and KEGG analyses. The target hub genes of NOB in Met S treatment were TP53, MAPK8, AKT1, GSK3B, HSP90AA1, CTNNB1, JUN, AR, ESR1, CCND1, HRAS, TNF, and PPARA. It was confirmed that lipid and atherosclerosis, together with the AGE-RAGE signaling pathway in diabetic complications, were putatively critical pathways of NOB in the treatment of metabolic syndrome. The molecular docking results revealed that most of 13 hub genes had a strong binding to NOB. Due to the versatile actions of NOB, it had the potential action on metabolic syndrome by multiple targets and multiple pathways.展开更多
基金the National Key Research and Development Program of China(2022YFB3305800-5)the National Natural Science Foundation of China(62125301,62021003)+2 种基金the Beijing Outstanding Young Scientist Program(BJJWZYJH01201910005020)the Natural Science Foundation of Beijing Municipality(KZ202110005009)Youth Beijing Scholar(037).
文摘In wastewater treatment systems,extracting meaningful features from process data is essential for effective monitoring and control.However,the multi-time scale data generated by different sampling frequencies pose a challenge to accurately extract features.To solve this issue,a multi-timescale feature extraction method based on adaptive entropy is proposed.Firstly,the expert knowledge graph is constructed by analyzing the characteristics of wastewater components and water quality data,which can illustrate various water quality parameters and the network of relationships among them.Secondly,multiscale entropy analysis is used to investigate the inherent multi-timescale patterns of water quality data in depth,which enables us to minimize information loss while uniformly optimizing the timescale.Thirdly,we harness partial least squares for feature extraction,resulting in an enhanced representation of sample data and the iterative enhancement of our expert knowledge graph.The experimental results show that the multi-timescale feature extraction algorithm can enhance the representation of water quality data and improve monitoring capabilities.
基金supported by the National Key Basic Research Program of China(2019YFF0301600 and 2020YFC2002900)National Natural Science Foundation of China(31930055,31871146,32071169,81870273,and 32071108)Major Basic Science Program of Shaanxi Provincial Natural Science Foundation of China(2016ZDJC-17).
文摘Background:Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects.Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs(miRNAs)after exercise and found that some of them act as exerkines.However,whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains unknown.Methods:A 9-day treadmill training was used as an exercise model in C57BL/6 mice.Liver-specific adeno-associated virus 8 was used to knock down microRNA-122-5p(miR-122-5p).Human umbilical vein endothelial cells were used in vitro.Results:Among these differentially expressed extracellular vesicle miRNAs,miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in vitro.Exercise increased circulating levels of miR-122-5p,which was produced mainly by the liver and shuttled by extracellular vesicles in mice.Inhibition of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles,and exerciseimproved muscle performance in mice.Mechanistically,miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells,and miR-122-5p upregulated endothelial cell fatty-acid utilization by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase(AGPAT1).In addition,miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in mice.Conclusion:These findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p,which enhances fatty acid utilization by targeting AGPAT1 in endothelial cells,highlighting the therapeutic potential of miR-122-5p in tissue repair.
基金financial support from the Science Foundation for Outstanding Young Scholars of Shandong Province (No. 2022HWYQ- 009)the Natural Science Foundation of Shandong Province (No. ZR2021QB201, No. YDZX2021001)the Qilu Young Scholars Program of Shandong University。
文摘Although a few pristine metal-organic frameworks(MOFs) of graphene analogue topology exhibit high intrinsic electrical conductivity, their use in lithium-ion batteries(LIBs) is still hampered by unfavorable Li+adsorption energy(ΔEa). In this paper, an electroconductive ferrocene-based MOF@MXene heterostructure is built to provide stable anodes for Li+storage. Charge density difference and planar average potential charge density show substantial redistribution of charges at the interfaces, transferring from MXene to MOF layers. Moreover, density functional theory(DFT) calculations reveal that the interaction between MXene and MOF significantly increases the ΔEa. As a result, the heterostructure anode exhibits high capacities and outstanding cycling stability with a capacity retention of 80% after 5000 cycles at 5 A g^(-1), outperforming mono-component MXene and MOF. Furthermore, the heterostructure anode is built into a full cell with a commercial NCM 532 cathode, delivering a high energy density of 611 Wh kg^(-1)and power density of 7600 W kg^(-1). The developed conductive MOF@MXene heterogeneity for improved LIB offers valuable insights into the design of advanced electrode materials for energy storage.
文摘North China Mountain Walnut (NCMW) is one of the ancestors of extant cultivated species, and a valuable gene resource for resistance breeding of walnut in China. Inter-Simple Sequence Repeat (ISSR) primers were designed to evaluate the level and pattern of genetic diversity in eight populations of NCMW. Nine ISSR primers yielded 91 amplification products with different sizes, of which 84 (92.31%) were polymorphic. A high species-level genetic diversity was detected with Nei’s (H = 0.2592) and Shannon’s diversity (I = 0.4003). In contrast, the population-level genetic diversity was relatively lower (PPB = 43.27%, H = 0.1347, I = 0.1862). Coefficient of populations differentiation (GST) was 0.5066, indicating that inter-population and intra-population variation contributed 50.66% and 49.34% respectively to the total genetic variability. This relative level of variation was further supported by AMOVA analysis. Limited gene flow (Nm = 0.5133.), habitat fragmentation and geographical isolation might be responsible for the population structure of NCMW. UPGMA cluster analysis classified the eight populations into three groups which showed no significant relationship between the genetic similarity coefficient and geographic origin but showed remarkable association with morpho-physiological characters, particularly nut traits. The results of the study provide species-level and population-level genetic profiles for further exploitation and conservation of genetic diversity of NCMW.
文摘Platelets aggregation and thrombosis formation are major reasons of cardiovascular and cerebral vascular diseases.To develop novel,effective and safe agents for inhibiting platelet aggregation and preventing above diseases is urgently needed.Some traditional Chinese medicines for“Houxue Huayu”have been shown to inhibit platelet aggregation potently.They are used frequently in China and also in Asian countries.Recently,the major effective components of Pueraria lobata,Salvia miltiorrhiza Bunge,Apium graveolens L.and so on were studied.The mechanisms and the molecular targets of puerarin,salvianolic acid B and the analogue of 3-n-butylphthalide,dl-PHPB were investigated.Four platelet aggregation inducers,ADP,arachidonic acid(AA),collagen(Col)and thrombin,were used in the study.
基金partially supported by National Key Research and Development Program of China(2019YFC1510902)National Natural Science Foundation of China(62073104)+1 种基金Natural Science Foundation of Heilongjiang Province(LH2022F024)China Postdoctoral Science Foundation(2022M710965)。
文摘This paper presents a subspace identification method for closed-loop systems with unknown deterministic disturbances.To deal with the unknown deterministic disturbances,two strategies are implemented to construct the row space that can be used to approximately represent the unknown deterministic disturbances using the trigonometric functions or Bernstein polynomials depending on whether the disturbance frequencies are known.For closed-loop identification,CCF-N4SID is extended to the case with unknown deterministic disturbances using the oblique projection.In addition,a proper Bernstein polynomial order can be determined using the Akaike information criterion(AIC)or the Bayesian information criterion(BIC).Numerical simulation results demonstrate the effectiveness of the proposed identification method for both periodic and aperiodic deterministic disturbances.
基金funded by the National Natural Science Foundation of China(No.31971400)the"Saving Spoon-billed Sandpiper"of Shenzhen Mangrove Wetlands Conservation Foundation(MCF)the Fundamental Research Funds for the Central Universities(No.BLX202144)。
文摘Nordmann's Greenshank(Tringa guttifer)is a globally endangered species that has received little research attention.It is threatened by rapid habitat loss,an incomplete network of protected sites,and lack of long-term data on population dynamics.Citizen science data can be combined with survey data to support population estimation and conservation gap analysis.From 2020 to 2021,Nordmann's Greenshank was surveyed in Tiaozini,Xiaoyangkou,and Dongling on the southern coast of Jiangsu Province,China,and the global population of the species was re-evaluated using the data obtained.We integrated citizen science data from eBird and the China Bird Report from 2000 to 2020 with the survey results to identify important habitats harboring over 1%of its total population,and compared this data with existing protected areas to identify gaps in its global conservation.Our survey found that Tiaozini supported at least 1194 individuals.Consequently,its global population was reestimated to be 1500-2000.Moreover,45 important habitats were identified based on citizen data and survey results.Although 44.4%and 50.0%of the priority sites in the world and China,respectively,are located outside protected areas,the Conservation Effectiveness Index(C)is 68.4%and 71.1%,respectively,showing that the current coverage of protected areas for this part of its range is reasonable.This study presents the most complete and recent population data to date.Tiaozini is the most important migration stopover site for Nordmann's Greenshanks.The species is under threat in terms of breeding,wintering,and stopover sites.Therefore,we suggest improving monitoring,establishing new protected sites to complete the habitat protection network,and improving the effectiveness of existing habitat protection strategies,including further developing high tide roosting sites.
基金Supported by National Spark Program(2013GA690123)Agricultural New Variety Postsubsidy Project of Major Research and Development Programof Jiangsu Province(BE2016398)~~
文摘The yield and yield components of Japonica variety Tongjing 981 under different density and fertilization levels were studied through regression and correlation and path analysis. The results showed that the number of panicles per unit area, number of filled grains per panicle and 1 000-grain weight all had very significant yield increasing effects, and the number of panicles per unit area played a leading role. However, the yield increasing effects of the number of panicles per unit area and number of filled grains per panicle are equally important when the basic seedlings are more or the N fertilizer application rate is large. In practical production, a major factor should be determined among the yield components, and rational cultivation measures should be taken accordingly, to improve yield.
基金Sponsored by the National Natural Science Foundation of China(Grant No.51206038)the Scientific Research Fund of Heilongjiang Provincial Education Department(Grant No.12521122)
文摘To correct the light scattering property parameters of milk fat for improving the detection accuracy,the Mie-theory was used to establish a predictive model for light scattering properties of milk fat globule with multi-diameter distributions, by means of Monte Carlo approach to simulate actual multi-diameter size distribution of milk fat globule in milk fat solution. Scattering coefficient and absorption coefficient of multidiameter distribution milk fat particles were calculated by simulating the particles size distribution in milk fat solution. And the light scattering properties of multi-diameter distribution was compared with that of volume mean diameter,Sauter mean diameter and numerical mean diameter in milk fat solution. Theoretical simulation results indicate that the scattering coefficient and absorption coefficient of milk fat particles are determined by the particle size distribution in milk fat solution. There is a distinct difference in scattering characteristics between the milk fat particles with multi-diameter distribution and that with mean diameters. Compared to that with multi-diameter distribution,the scattering coefficient of the milk fat particles with mean diameter has a maximum mean deviation of 9042 m-1. The particle size distribution is not completely determined by the mean diameters. The dependence of the light scattering properties on the particle size distribution should be considered into the model and simulation. Therefore,it is found that the particle size distribution in milk fat solution is an essential and critical factor to significantly improve the detection accuracy of milk fat content.
基金the Main Project of Ministry of Education of China (No. 207150)
文摘Objective: To construct the small interfering RNA (siRNA) expression vector of carcino-embryonic antigen (CEA) and inhibit the expression of CEA in EC9706 cells by RNA interference. Methods: Two pairs of oligonucleotide sequences were designed and synthesized according to the encoding sequence of mRNA of CEA. The annealed oligonucleotide frag-ments were cloned into pRNAT-U6.2 expression vector and identified by sequencing. The recombinant plasmid pRNAT-U6.2-CEA was transfected into EC9706 cells. The expression of CEA in the stable transfected cells was assayed by real time PCR and Western blot. Results: DNA sequencing showed that the oligonucleotide fragments were correctly inserted into pRNAT-U6.2 vector, and CEA expression in the transfected cells was down-regulated significantly by pRNAT-U6.2-CEA at both the mRNA and protein levels. Conclusion: The siRNA expression vector of CEA is successfully constructed and inhibits CEA expression in EC9706 cells. This facilitates further studies of the function of CEA at the molecular level.
基金supported by the National Natural Science Foundation of China(22304062)the Zhejiang Provincial Natural Science Foundation of China(LTGY24B050002)+2 种基金the Program for Science and Technology of Jiaxing(2023AY40028)the Baiqing Foundation of Jiaxing University(CD70621010)Springer Nature or its licensor(e.g.a society or other partner)holds exclusive rights to this article under a publishing agreement with the author(s)or other rightsholder(s)author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law。
文摘In this work,we proposed a ratiometric silver nanoclusters(AgNCs)fluorescent assay by designing a bifunctional-blockeraided hybridization chain reaction(HCR).Hairpin probe 1(HP1)containing two special DNA fragments(5′-CAC CGC T-3′and 5′-ATT TGC CTT TTG GGG ACG GATA-3′)at two terminals creates a red-emitting AgNC nucleation sequence(rNS,5′-CAC CGC TAT TTG CCT TTT GGG GAC GGATA-3′).We found that the presence of a toehold fragment(5′-TGCCC-3′)in HP1 could silence the rNS.Upon the addition of a target nucleic acid,HCR of HP1 and hairpin probe 2(HP2)could be initiated,resulting in the formation of long chain of DNA duplexes with multibranched rNS.As the toehold fragment in HP1participated in generating duplexes,a strong emission of rNS-templated AgNCs was observed at 670 nm.More significantly,a bifunctional blocker was introduced not only to reduce the background red-emitting fluorescence but also to play as an internal green-emitting AgNCs nucleation sequence.On the one hand,the blocker could increase the signal-to-noise-ratio of the constructed biosensor,and on the other hand,the blocker also helped to prepare ratiometric HCR-AgNCs assay with self-calibrating ability to strengthen its reproducibility.Compared with the traditional HCR-AgNCs sensors,the developed ratiometric assay based on the bifunctional-blocker-aided HCR has higher reliability,which is important for the fabrication of biosensors in various fields for practical biosensing applications.
基金supported by the National Natural Science Foundation of China(22304062)the Zhejiang Provincial Natural Science Foundation of China(LTGY24B050002)+1 种基金the Program for Science and Technology of Jiaxing(2023AY40028)the Baiqing Foundation of Jiaxing University(CD70621010).
文摘Although immobilization-free and label-free electrochemical DNA(E-DNA)biosensors have engaged tremendous interest due to their superior properties,such as easy operation,time-saving and cost-saving,most of them are fabricated in homogeneous modes and usually produce high background current.In the present work,we proposed a new immobilization-free and label-free heterogeneous E-DNA assay based on a dual-blocker-aided multibranched hybridization chain reaction(HCR)for one-pot nucleic acid detection with zero background.The target nucleic acid triggers the HCR involving cascaded hybridization between two metastable hairpins,resulting in the generation of HCR products with multibranched arms,which can be captured onto the electrode viaπ-πstacking interactions between multibranched arms and reduced graphene oxide(rGO).Prior to the incubation process with an electrode,two blockers are designed to prohibit the nonspecific absorption of unreacted hairpin probes.Thus,an immobilization-free and label-free heterogeneous electrochemical assay for one-pot nucleic acid detection with zero background is readily realized.This strategy also presents additional merits of simplicity and cheap cost,since probe immobilization,signal tag labeling,and multiple incubation processes are avoided.Therefore,the as-proposed effective and versatile biosensor has great potential to be applied in nucleic acid-related practical biosensing.
基金This study was supported by the National Natural Science Foundation of China(Grant Number:41705122).
文摘Background and Aims:Metabolic dysfunction-associ-ated fatty liver disease(MAFLD)is a new concept,pro-posed in 2020;however,its applicability in Asia populations has yet to be evaluated.Therefore,we aimed to compare the difference in epidemiological and clinical characteris-tics between MAFLD and non-alcoholic fatty liver disease(NAFLD)among Asian populations.Methods:Based on the Jinchang cohort,30,633 participants were collected.The prevalence and incidence of MAFLD and NAFLD were used to analyze the epidemic characteristics and its overlapping effects.In addition,the corresponding clinical character-istics of the two diagnostic criteria populations were com-pared.Results:The prevalence rates of MAFLD and NAFLD were 21.03%and 18.83%,respectively.After an average 2.28-year follow-up,the incidence densities of MAFLD and NAFLD were 41.58 per 1,000 person-years and 37.69 per 1,000 person-years,respectively.With the increase of baseline age,body mass index(BMI),and waist circumfer-ence(WC)levels,the prevalence and incidence of MAFLD and NAFLD were on the rise(all ptrend<0.05).Among the total patients diagnosed at baseline or follow-up,most pa-tients had both MAFLD and NAFLD,accounting for 78.84%and 82.88%,respectively.Compared with NAFLD,MAFLD patients had greater proportions of males and metabolic diseases(diabetes,dyslipidemia),and had higher BMI,WC,liver enzymes,blood glucose,and lipid levels in the base-line diagnosis patients(p<0.05).Additionally,lean MAFLD patients had higher metabolic disorders than lean NAFLD patients(p<0.05).Conclusions:Compared with NAFLD,the newly proposed definition of MAFLD is more practical and accurate,and it can help identify more fatty liver pa-tients with high-risk diseases.
基金This work is partially supported by the National Natural Science Foundation of China(Grant Nos.61465004 and 61765004)the Guangxi Natural Science Foundation(Grant Nos.2017GXNSFAA1981642016GXNSFAA380006)the Guangxi Key Laboratory of Precision Navigation Technology and Application,Guilin University of Electronic Technology Foundation(Grant No.DH201804).
文摘A finite-difference-time-domain(FDTD)approach is undertaken to investigate the extraordinary optical transmission(EOT)phenomenon of Au circular aperture arrays deposited on a Bragg fiber facet for refractive index(RI)sensing.Investigation shows that the choice of effective indices and modal loss of the Bragg fiber core modes will affect the sensitivity enhancement by using a mode analysis approach.The critical parameters of Bragg fiber including the middle dielectric RI,as well as its gap between dielectric layers,which affect the EOT and RI sensitivity for the sensor,are discussed and optimized.It is demonstrated that a better sensitivity of 156±5 nm per refractive index unit(RIU)and an averaged figure of merit exceeding 3.5 RIU‒1 are achieved when RI is 1.5 and gap is 0.02μm in this structure.
基金This work was supported by the National Key Research and Development Project(No.2018YFC1900800-5)National Science Foundation of China(Nos.61890930-5,61903010,62021003,and 62125301)+2 种基金Beijing Outstanding Young Scientist Program(No.BJJWZYJH01201910005020)Beijing Natural Science Foundation(No.KZ202110005009)CAAI-Huawei MindSpore Open Fund(No.CAAIXSJLJJ-2021-017A).
文摘Seeking continuous development,a modern community must also be able to adapt to future possible challenges using constrained or limited resources.As a revolutionary communication paradigm,the Internet of Things(IoT)empowers the cutting-edge and emerging applications which enable manifold new intelligent services towards a smart community.The sophisticated ecosystem of a digital community is made feasible by the IoT infrastructure,which also provides community control with access to a wealth of actual data.In addition,IoT platforms empower the ubiquitous computing ability,providing more potentials to the actuators in perception layer in the IoT architecture.With more and more population in the urban areas,sustainability issues have become a key factor to consider in the development of a digital community.We give a modern survey in this study on the most recent developments in IoT for sustainable digital communities.After carefully examining the most recent literature,we specifically highlight the various smart digital community application scenarios,such as smart buildings,energy management,green transportation,trash management,etc.We also look into a number of major issues facing the use of IoT technology in digital communities.Furthermore,we discuss potential future applications and future research areas for IoT,the critical component of sustainable digital communities.
基金This research was supported by the National Natural Science Foundation of China(Grant nos.41476111 and 31372529).
文摘Agarophyton vermiculophyllum is an agarophytic red alga originating from Northeast Asia that successfully spread to Europe and North America in the last 20 years.In this study,three hsp70 genes(hsp70-l,hsp70-2,and hsp70-3)and one hsp90 gene were cloned,and real-time reverse transcription polymerase chain reaction(RT-qPCR)was used to measure their transcriptional levels in three kinds of thalli(tetrasporophytes,and male/female gametophytes)belonging to diploid or haploid phases in the life cycle of A.vermiculophyllum.The results show that the three Hsp70s in A.vermiculophyllum clustered into three different groups,and the locations of the putative Hsp70-l,Hsp70-2,and Hsp70-3 were in the cytoplasm,endoplasmic reticulum and chloroplast,respectively,according to their sequences.Beside on the same research,the putative Hsp90 was supposed to have a cytoplasmic location.The RT-qPCR results show that the three hsp70 genes were highly upregulated in gametophytes as compared to tetrasporophytes but the transcriptional level of hsp90 did not show such a significant increase.The chloroplast hsp70-3 exhibited the highest upregulation and the transcriptional level in creased more than 570 fold in female gametophytes,and 17 fold in male gametophytes,compared with tetrasporophytes.Therefore,cpHsp70-3 might act more like a chaperon molecule involved in haploid development under natural condition,while Hsp70-l and Hsp70-2 were more active in stress resistance.
基金Basic Scientific Research Operating Expenses of Heilongjiang Provincial Universities (Grant No. 2018-KYYWF-0100)。
文摘Nobiletin(NOB) may have a potential effect on metabolic syndrome. In the present study, we aimed to explore the latent mechanisms of NOB for the treatment of metabolic syndrome based on network pharmacology and molecular docking methods. The potential targets of NOB were retrieved and identified from six databases, such as the Traditional Chinese Medicine Systems Pharmacology Database. The metabolic syndrome-related targets were retrieved from six databases as follows: the Drug Bank database, Gene Cards database, the Online Mendelian Inheritance in Man database, Pharm GKB database, Therapeutic Target Database, and Dis Ge Net database. Gene ontology(GO) analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis of 60 intersected genes were performed in R software(Bioconductor, cluster Profiler) to investigate the molecular mechanisms. Subsequently, the ingredient-target-pathway network of NOB was constructed and visualized through Cytoscape 3.8.0 software. Protein-protein interaction network was constructed to screen hub genes in the treatment of NOB on metabolic syndrome through The Search Tool for the Retrieval of Interacting Genes/Proteins and visualized by Cytoscape 3.8.0 software. Afterward, molecular docking was used to analyze the score of the hub genes with NOB. Cumulatively, 105 targets of NOB were identified. Moreover, 1975 metabolic syndrome-related genes were acquired from six databases after combining and deleting the repeated items, and the overlap of metabolic syndrome-related genes with NOB-related target genes identified 60 intersection genes of NOB against metabolic syndrome. Moreover, 1858 GO entries of NOB on metabolic syndrome were identified, and 153 pathways were screened based on GO and KEGG analyses. The target hub genes of NOB in Met S treatment were TP53, MAPK8, AKT1, GSK3B, HSP90AA1, CTNNB1, JUN, AR, ESR1, CCND1, HRAS, TNF, and PPARA. It was confirmed that lipid and atherosclerosis, together with the AGE-RAGE signaling pathway in diabetic complications, were putatively critical pathways of NOB in the treatment of metabolic syndrome. The molecular docking results revealed that most of 13 hub genes had a strong binding to NOB. Due to the versatile actions of NOB, it had the potential action on metabolic syndrome by multiple targets and multiple pathways.
