BACKGROUND China's most frequent malignancy is gastric cancer(GC),which has a very poor survival rate,and the survival rate for patients with advanced GC is dismal.Pyroptosis has been connected to the genesis and ...BACKGROUND China's most frequent malignancy is gastric cancer(GC),which has a very poor survival rate,and the survival rate for patients with advanced GC is dismal.Pyroptosis has been connected to the genesis and development of cancer.The function of pyroptosis-related long non-coding RNAs(PRLs)in GC,on the other hand,remains uncertain.AIM To explore the construction and comprehensive analysis of the prognostic characteristics of long non-coding RNA(lncRNA)related to pyroptosis in GC patients.METHODS The TCGA database provided us with 352 stomach adenocarcinoma samples,and we obtained 28 pyroptotic genes from the Reactome database.We examined the correlation between lncRNAs and pyroptosis using the Pearson correlation coefficient.Prognosis-related PRLs were identified through univariate Cox analysis.A predictive signature was constructed using stepwise Cox regression analysis,and its reliability and independence were assessed.To facilitate clinical application,a nomogram was created based on this signature.we analyzed differences in immune cell infiltration,immune function,and checkpoints between the high-risk group(HRG)and low-risk group(LRG).RESULTS Five hundred and twenty-three PRLs were screened from all lncRNAs(absolute correlation coefficient>0.4,P<0.05).Nine PRLs were included in the risk prediction signature that was created through stepwise Cox regression analysis.We determined the risk score for GC patients and employed the median value as the dividing line between HRG and LRG.The ability of the risk signature to predict the overall survival(OS)of GC is demonstrated by the Kaplan-Meier analysis,risk curve,receiver operating characteristic curve,and decision curve analysis curve.The risk signature was shown to be an independent prognostic factor for OS in both univariate and multivariate Cox regression analyses.HRG showed a more efficient local immune response or modulation compared to LRG,as indicated by the predicted signal pathway analysis and examination of immune cell infiltration,function,and checkpoints(P<0.05).CONCLUSION In general,we have created a brand-new prognostic signature using PRLs,which may provide ideas for immunotherapy in patients with GC.展开更多
Background Endothelial function,as measured by big endothelin-1(ET-1),has been demonstrated to be useful in predicting adverse long-term events in patients with cardiovascular disease.Nevertheless,there are little dat...Background Endothelial function,as measured by big endothelin-1(ET-1),has been demonstrated to be useful in predicting adverse long-term events in patients with cardiovascular disease.Nevertheless,there are little data about the association between big ET-1 and thromboembolism risk in atrial fibrillation(AF).We aimed to investigate the relationship between big ET-1 and CHADS2/CHA2DS2-VASc scores used for evaluating thromboembolic risk in patients with non-valvular AF.Methods The study population consisted of 238 consecutive AF patients(67.6%with paroxysmal AF and 32.4%with persistent AF).The patients were divided into two groups(high-or low-intermediate risk group)based on CHADS2 and CHA2 DS2-VASc scores(score≥2 or<2,respectively).Clinical,laboratory,and echocardiographic parameters were evaluated,and the CHADS2/CHA2DS2-VASc scores were compared between groups.The association between big ET-1 levels and CHADS2/CHA2DS2-VASc score was assessed.Multivariate logistic regression analysis was performed to identify independent predictors of CHADS2/CHA2DS2-VASc scores.Results The high CHADS2/CHA2DS2-VASc score group had older age,higher big ET-1 levels,and enlarged left atrial diameter than the low CHADS2/CHA2DS2-VASc score group(P<0.05).Multiple logistic regression analysis revealed that big ET-1 level was an independent determinant of high CHADS2/CHA2DS2-VASc scores[odds ratio(OR)=2.545 and OR=3.816;both P<0.05].Conclusions Our study indicates that in non-valvular AF,big ET-1 was significantly correlated with CHADS2/CHA2DS2-VASc scores and an independent predictor of high CHADS2/CHA2DS2-VASc scores.Big ET-1 may serve as a useful marker for risk stratification in this setting.展开更多
An equiatomic FeSiBAlNi amorphous high-entropy alloy （HEA） was fabricated by mechanical alloying （MA）. A fully amorphous phase was obtained in the FeSiBAlNi HEA after 240 h of MA. The bulk FeSiBAlNi samples were s...An equiatomic FeSiBAlNi amorphous high-entropy alloy （HEA） was fabricated by mechanical alloying （MA）. A fully amorphous phase was obtained in the FeSiBAlNi HEA after 240 h of MA. The bulk FeSiBAlNi samples were sintered by spark plasma sintering （SPS） at 520 and 1080℃ under a pressure of 80 MPa. The sample sintered at 520℃ exhibited an amorphous composite structure comprising solid-solution phases （body-centered cubic （bcc） and face-centered cubic （fee） phases）. When the as-milled amorphous HEA was consoli- dated at 1080℃, another fcc phase appeared and the amorphous phase disappeared. The sample sintered by SPS at 1080℃ exhibited a slightly higher melting temperature compared with those of the as-milled alloy and the bulk sample sintered at 520℃. The corrosion behav- iors of the as-sintered samples were investigated by potentiodynamic polarization measurements and immersion tests in seawater solution. The results showed that the HEA obtained by SPS at 1080℃ exhibited better corrosion resistance than that obtained by SPS at 520℃.展开更多
The mechanical performances such as tensile strength and blast property of metal lined SiC/SiC composite cladding tubes were investigated. Nb or Ta was selected as liner material, and the SiC/SiC composite layer was f...The mechanical performances such as tensile strength and blast property of metal lined SiC/SiC composite cladding tubes were investigated. Nb or Ta was selected as liner material, and the SiC/SiC composite layer was fabricated by winding and different precursor impregnation and pyrolysis(PIP) processes. The tensile strengths of different tube samples were measured at room temperature(RT) and 1200 °C, respectively. The blast property was investigated through the maximum water pressure of tubes. And the fracture microstructures were observed by SEM.The highest tensile strength at RT was 150.7 MPa. The blast strength was enhanced with the PIP process increasing from 1 to 4 cycles and the tube of 4 PIP cycles had the highest water pressure of 34.7 MPa. Compared with the metal tubes, the multi-layer structure improved tensile and blast properties significantly. The different processes such as PIP cycles and pyrolytic carbon(PyC) coating were important factors to enhance the mechanical performances of SiC/SiC-based tubes. However, the retention rate of tensile strength was only 18.5% at 1200 °C.展开更多
Size-classified daily aerosol mass concentrations and concentrations of water-soluble inorganic ions were measured in Hefei,China,in four representative months between September 2012 and August 2013.An annual average ...Size-classified daily aerosol mass concentrations and concentrations of water-soluble inorganic ions were measured in Hefei,China,in four representative months between September 2012 and August 2013.An annual average mass concentration of 169.09μg/m^3 for total suspended particulate（TSP）was measured using an Andersen Mark-II cascade impactor.The seasonal average mass concentration was highest in winter（234.73μg/m^3）and lowest in summer（91.71μg/m^3）.Water-soluble ions accounted for 59.49%,32.90%,48.62%and 37.08%of the aerosol mass concentration in winter,spring,summer,and fall,respectively,which indicated that ionic species were the primary constituents of the atmospheric aerosols.The four most abundant ions were NO3^-,SO4^2-,Ca^2＋ and NH4^＋.With the exception of Ca^2＋,the mass concentrations of water-soluble ions were in an intermediate range compared with the levels for other Chinese cities.Sulfate,nitrate,and ammonium were the dominant fine-particle species,which were bimodally distributed in spring,summer and fall;however,the size distribution became unimodal in winter,with a peak at 1.1–2.1μm.The Ca^2＋ peak occurred at approximately 4.7–5.8μm in all seasons.The cation to anion ratio was close to 1.4,which suggested that the aerosol particles were alkalescent in Hefei.The average NO3^-/SO4^2-mass ratio was 1.10 in Hefei,which indicated that mobile source emissions were predominant.Significant positive correlation coefficients between the concentrations of NH4^＋ and SO4^2-,NH4^＋ and NO3^-,SO4^2-and NO3^-,and Mg^2＋ and Ca^2＋ were also indicated,suggesting that aerosol particles may be present as（NH4）2SO4,NH4HSO4,and NH4NO3.展开更多
Hexane, petroleum ether, chloroform, ethyl acetate, methanol and water extracts of Clausena anisata [（Willd.） Hook E Ex Benth] leaves and roots were evaluated against Helicoverpa armigera （Hubner） for antifeedant ...Hexane, petroleum ether, chloroform, ethyl acetate, methanol and water extracts of Clausena anisata [（Willd.） Hook E Ex Benth] leaves and roots were evaluated against Helicoverpa armigera （Hubner） for antifeedant activities. Antifeedant activity was confirmed, and was found to be higher in root extracts than those of the leaf. Chloroform and petroleum ether extracts of the root showed strongest antifeedant activities （DC50s [concentration （C） causing 50% deterrence compared with the control] 0.014% and 0.016% respectively）, and root extracts were fractionated using silica gel column chromatography. One fraction of the chloroform and one of the petroleum ether root extracts was active; and on the basis of mass spectroscopy and ^1H and ^13C nuclear magnetic resonance spectral data, the active compounds in the two fractions were confirmed to be identical, and identified as osthol [2H-1-Benzopyran-2-one, 7-methoxy-8-（3-methyl-2-butenyl）]. The highest concentration of osthol was found in the chloroform root extract. Antifeedant activities of the root extracts, as measured by DC50 values, were highly correlated with their osthol contents. Approximately 99% of the variation in bioactivity of the root extracts could be accounted for by variation in osthol content; osthol therefore, appeared to be an antifeedant component of C. anisata to H. armigera. This may provide a new approach to managing this pest.展开更多
Background The Beijing Electron Positron Collider(BEPCII)is a high-brightness collider and operates in compatible mode of synchronous light source.This summer,BEPCII spare cavity,which was all made in China,had been p...Background The Beijing Electron Positron Collider(BEPCII)is a high-brightness collider and operates in compatible mode of synchronous light source.This summer,BEPCII spare cavity,which was all made in China,had been put into BEPCII operation,and the LLRF system for the spare cavity was also upgraded at the time.Purpose The purpose is to stably control the amplitude and phase of the spare cavity and apply digital technology to achieve some intelligent functions.Methods The digital method is applied in the superconducting cavity feedback control of the accelerator and it introduces the digital technology of the RF low-level control system that has been stably applied to the BEPCII spare cavity operation.In addition,some functions such as quench detector and input protection as well as horizontal test have been realized in the RF system operation.Results The amplitude and phase stabilities of cavity voltage with beam in the digital LLRF have reached±0.09%(RMS)and±0.055°(RMS),respectively.Conclusion The BEPCII spare cavity with digital LLRF system has been stably operated for 2 months.The DLLRF system has been reliable and stable,and the performance of the spare cavity with beam was very good.展开更多
Tumor metastasis accounts for over 90%of tumor-related deaths,prompting the development of fluorescently labeled tumor-specific molecular imaging agents for differentiating tumors from normal tissues.However,early det...Tumor metastasis accounts for over 90%of tumor-related deaths,prompting the development of fluorescently labeled tumor-specific molecular imaging agents for differentiating tumors from normal tissues.However,early detection of metastasis lesions by tracking tumor markers alone has proven to be challenging.Herein,we reported a glycopeptide-based bispecific fluorescence probe(bsProbe)for earlier detection of bladder cancer and metastasis.By simultaneously recognition(tumor&tumor microenvironment)and in vivo self-assembly,the tumor accumulation of bsProbe(12.3%ID/g)was obviously increased by∼6 fold compared with that in CXCR4 specific fluorescence probe(sProbe),indicating the obvious advantages of bsProbe over existing tumor metastasis detection probes.Additionally,bsProbe substantially broadens the tumor diagnosis window and enhances the detection signal to noise ratio(SNR:approximately 9.5),permitting early diagnosis of lung micro-metastasis(∼1 mm),precise identifying of tumor boundaries and micro-tumors in orthotopic tumor models.More importantly,bsProbe was demonstrated to distinguish malignant from benign specimen with a specificity of 90.48%and sensitivity of 92.22%in 195 clinical specimens of bladder cancer patients.Taken together,this novel synergetic targeting(CD206×CXCR4)strategy provides an attractive method for earlier detection of bladder cancer and metastasis,which might be further extended to the imaging-guided surgery of clinical invisible tumors.展开更多
基金Supported by The Scientific Research Project of Integrated Traditional Chinese and Western Medicine of Tianjin Health Commission Administration of Traditional Chinese Medicine,No.2021010 and No.2023166Xiao-Ping Chen Foundation for the Development of Science and Technology of Hubei Province,No.CXPJJH122002-073.
文摘BACKGROUND China's most frequent malignancy is gastric cancer(GC),which has a very poor survival rate,and the survival rate for patients with advanced GC is dismal.Pyroptosis has been connected to the genesis and development of cancer.The function of pyroptosis-related long non-coding RNAs(PRLs)in GC,on the other hand,remains uncertain.AIM To explore the construction and comprehensive analysis of the prognostic characteristics of long non-coding RNA(lncRNA)related to pyroptosis in GC patients.METHODS The TCGA database provided us with 352 stomach adenocarcinoma samples,and we obtained 28 pyroptotic genes from the Reactome database.We examined the correlation between lncRNAs and pyroptosis using the Pearson correlation coefficient.Prognosis-related PRLs were identified through univariate Cox analysis.A predictive signature was constructed using stepwise Cox regression analysis,and its reliability and independence were assessed.To facilitate clinical application,a nomogram was created based on this signature.we analyzed differences in immune cell infiltration,immune function,and checkpoints between the high-risk group(HRG)and low-risk group(LRG).RESULTS Five hundred and twenty-three PRLs were screened from all lncRNAs(absolute correlation coefficient>0.4,P<0.05).Nine PRLs were included in the risk prediction signature that was created through stepwise Cox regression analysis.We determined the risk score for GC patients and employed the median value as the dividing line between HRG and LRG.The ability of the risk signature to predict the overall survival(OS)of GC is demonstrated by the Kaplan-Meier analysis,risk curve,receiver operating characteristic curve,and decision curve analysis curve.The risk signature was shown to be an independent prognostic factor for OS in both univariate and multivariate Cox regression analyses.HRG showed a more efficient local immune response or modulation compared to LRG,as indicated by the predicted signal pathway analysis and examination of immune cell infiltration,function,and checkpoints(P<0.05).CONCLUSION In general,we have created a brand-new prognostic signature using PRLs,which may provide ideas for immunotherapy in patients with GC.
基金supported by the National Natural Science Foundation of China(No.81600275)
文摘Background Endothelial function,as measured by big endothelin-1(ET-1),has been demonstrated to be useful in predicting adverse long-term events in patients with cardiovascular disease.Nevertheless,there are little data about the association between big ET-1 and thromboembolism risk in atrial fibrillation(AF).We aimed to investigate the relationship between big ET-1 and CHADS2/CHA2DS2-VASc scores used for evaluating thromboembolic risk in patients with non-valvular AF.Methods The study population consisted of 238 consecutive AF patients(67.6%with paroxysmal AF and 32.4%with persistent AF).The patients were divided into two groups(high-or low-intermediate risk group)based on CHADS2 and CHA2 DS2-VASc scores(score≥2 or<2,respectively).Clinical,laboratory,and echocardiographic parameters were evaluated,and the CHADS2/CHA2DS2-VASc scores were compared between groups.The association between big ET-1 levels and CHADS2/CHA2DS2-VASc score was assessed.Multivariate logistic regression analysis was performed to identify independent predictors of CHADS2/CHA2DS2-VASc scores.Results The high CHADS2/CHA2DS2-VASc score group had older age,higher big ET-1 levels,and enlarged left atrial diameter than the low CHADS2/CHA2DS2-VASc score group(P<0.05).Multiple logistic regression analysis revealed that big ET-1 level was an independent determinant of high CHADS2/CHA2DS2-VASc scores[odds ratio(OR)=2.545 and OR=3.816;both P<0.05].Conclusions Our study indicates that in non-valvular AF,big ET-1 was significantly correlated with CHADS2/CHA2DS2-VASc scores and an independent predictor of high CHADS2/CHA2DS2-VASc scores.Big ET-1 may serve as a useful marker for risk stratification in this setting.
基金the Natural Science Foundation of China (No. 51171072)the Excellent Middle-age and Young Scientists Research Award Foundation of Shandong Province (No. BS2012CL002)the International Cooperation Training Project of Excellent Young and Middle-aged Teachers of Shandong Province, China
文摘An equiatomic FeSiBAlNi amorphous high-entropy alloy （HEA） was fabricated by mechanical alloying （MA）. A fully amorphous phase was obtained in the FeSiBAlNi HEA after 240 h of MA. The bulk FeSiBAlNi samples were sintered by spark plasma sintering （SPS） at 520 and 1080℃ under a pressure of 80 MPa. The sample sintered at 520℃ exhibited an amorphous composite structure comprising solid-solution phases （body-centered cubic （bcc） and face-centered cubic （fee） phases）. When the as-milled amorphous HEA was consoli- dated at 1080℃, another fcc phase appeared and the amorphous phase disappeared. The sample sintered by SPS at 1080℃ exhibited a slightly higher melting temperature compared with those of the as-milled alloy and the bulk sample sintered at 520℃. The corrosion behav- iors of the as-sintered samples were investigated by potentiodynamic polarization measurements and immersion tests in seawater solution. The results showed that the HEA obtained by SPS at 1080℃ exhibited better corrosion resistance than that obtained by SPS at 520℃.
基金supported by the National Key R&D Program of China (No. 2018YFB1900603)the Natural Science Foundation of Hunan Province, China (No. 2020JJ4667)。
文摘The mechanical performances such as tensile strength and blast property of metal lined SiC/SiC composite cladding tubes were investigated. Nb or Ta was selected as liner material, and the SiC/SiC composite layer was fabricated by winding and different precursor impregnation and pyrolysis(PIP) processes. The tensile strengths of different tube samples were measured at room temperature(RT) and 1200 °C, respectively. The blast property was investigated through the maximum water pressure of tubes. And the fracture microstructures were observed by SEM.The highest tensile strength at RT was 150.7 MPa. The blast strength was enhanced with the PIP process increasing from 1 to 4 cycles and the tube of 4 PIP cycles had the highest water pressure of 34.7 MPa. Compared with the metal tubes, the multi-layer structure improved tensile and blast properties significantly. The different processes such as PIP cycles and pyrolytic carbon(PyC) coating were important factors to enhance the mechanical performances of SiC/SiC-based tubes. However, the retention rate of tensile strength was only 18.5% at 1200 °C.
基金supported by the Anhui Provincial Natural Science Foundation(No.1308085MD55)the China Special Fund for Meteorological Research in the Public Interest(NosGYHY201206011 and GYHY201406039)
文摘Size-classified daily aerosol mass concentrations and concentrations of water-soluble inorganic ions were measured in Hefei,China,in four representative months between September 2012 and August 2013.An annual average mass concentration of 169.09μg/m^3 for total suspended particulate（TSP）was measured using an Andersen Mark-II cascade impactor.The seasonal average mass concentration was highest in winter（234.73μg/m^3）and lowest in summer（91.71μg/m^3）.Water-soluble ions accounted for 59.49%,32.90%,48.62%and 37.08%of the aerosol mass concentration in winter,spring,summer,and fall,respectively,which indicated that ionic species were the primary constituents of the atmospheric aerosols.The four most abundant ions were NO3^-,SO4^2-,Ca^2＋ and NH4^＋.With the exception of Ca^2＋,the mass concentrations of water-soluble ions were in an intermediate range compared with the levels for other Chinese cities.Sulfate,nitrate,and ammonium were the dominant fine-particle species,which were bimodally distributed in spring,summer and fall;however,the size distribution became unimodal in winter,with a peak at 1.1–2.1μm.The Ca^2＋ peak occurred at approximately 4.7–5.8μm in all seasons.The cation to anion ratio was close to 1.4,which suggested that the aerosol particles were alkalescent in Hefei.The average NO3^-/SO4^2-mass ratio was 1.10 in Hefei,which indicated that mobile source emissions were predominant.Significant positive correlation coefficients between the concentrations of NH4^＋ and SO4^2-,NH4^＋ and NO3^-,SO4^2-and NO3^-,and Mg^2＋ and Ca^2＋ were also indicated,suggesting that aerosol particles may be present as（NH4）2SO4,NH4HSO4,and NH4NO3.
文摘Hexane, petroleum ether, chloroform, ethyl acetate, methanol and water extracts of Clausena anisata [（Willd.） Hook E Ex Benth] leaves and roots were evaluated against Helicoverpa armigera （Hubner） for antifeedant activities. Antifeedant activity was confirmed, and was found to be higher in root extracts than those of the leaf. Chloroform and petroleum ether extracts of the root showed strongest antifeedant activities （DC50s [concentration （C） causing 50% deterrence compared with the control] 0.014% and 0.016% respectively）, and root extracts were fractionated using silica gel column chromatography. One fraction of the chloroform and one of the petroleum ether root extracts was active; and on the basis of mass spectroscopy and ^1H and ^13C nuclear magnetic resonance spectral data, the active compounds in the two fractions were confirmed to be identical, and identified as osthol [2H-1-Benzopyran-2-one, 7-methoxy-8-（3-methyl-2-butenyl）]. The highest concentration of osthol was found in the chloroform root extract. Antifeedant activities of the root extracts, as measured by DC50 values, were highly correlated with their osthol contents. Approximately 99% of the variation in bioactivity of the root extracts could be accounted for by variation in osthol content; osthol therefore, appeared to be an antifeedant component of C. anisata to H. armigera. This may provide a new approach to managing this pest.
文摘Background The Beijing Electron Positron Collider(BEPCII)is a high-brightness collider and operates in compatible mode of synchronous light source.This summer,BEPCII spare cavity,which was all made in China,had been put into BEPCII operation,and the LLRF system for the spare cavity was also upgraded at the time.Purpose The purpose is to stably control the amplitude and phase of the spare cavity and apply digital technology to achieve some intelligent functions.Methods The digital method is applied in the superconducting cavity feedback control of the accelerator and it introduces the digital technology of the RF low-level control system that has been stably applied to the BEPCII spare cavity operation.In addition,some functions such as quench detector and input protection as well as horizontal test have been realized in the RF system operation.Results The amplitude and phase stabilities of cavity voltage with beam in the digital LLRF have reached±0.09%(RMS)and±0.055°(RMS),respectively.Conclusion The BEPCII spare cavity with digital LLRF system has been stably operated for 2 months.The DLLRF system has been reliable and stable,and the performance of the spare cavity with beam was very good.
基金supported by the National Key R&D Program of China(2022YFA1205701 and 2020YFA0210800)the National Natural Science Foundation of China(52322308,51725302,52273126,11621505,and 82302266)+8 种基金Beijing Nova Program(20230484237)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB36000000)the Key Research and Development Program of Heilongjiang Province(2022zX06004)Hong Kong scholar project(Xj2024052)Harbin Medical University Cancer Hospital Haiyan Foundation(JYQ2024-03 and JZD2024-17)the Nn10 program of Harbin Medical University Cancer Hospital(Nn102024-01)the Climing program of Harbin Medical University Cancer Hospital(PDYS2024-03)China Postdoctoral Science Foundation(2024MD753933)Heilongjiang Postdoctoral Fund(LBH-Z23221).
文摘Tumor metastasis accounts for over 90%of tumor-related deaths,prompting the development of fluorescently labeled tumor-specific molecular imaging agents for differentiating tumors from normal tissues.However,early detection of metastasis lesions by tracking tumor markers alone has proven to be challenging.Herein,we reported a glycopeptide-based bispecific fluorescence probe(bsProbe)for earlier detection of bladder cancer and metastasis.By simultaneously recognition(tumor&tumor microenvironment)and in vivo self-assembly,the tumor accumulation of bsProbe(12.3%ID/g)was obviously increased by∼6 fold compared with that in CXCR4 specific fluorescence probe(sProbe),indicating the obvious advantages of bsProbe over existing tumor metastasis detection probes.Additionally,bsProbe substantially broadens the tumor diagnosis window and enhances the detection signal to noise ratio(SNR:approximately 9.5),permitting early diagnosis of lung micro-metastasis(∼1 mm),precise identifying of tumor boundaries and micro-tumors in orthotopic tumor models.More importantly,bsProbe was demonstrated to distinguish malignant from benign specimen with a specificity of 90.48%and sensitivity of 92.22%in 195 clinical specimens of bladder cancer patients.Taken together,this novel synergetic targeting(CD206×CXCR4)strategy provides an attractive method for earlier detection of bladder cancer and metastasis,which might be further extended to the imaging-guided surgery of clinical invisible tumors.
