The recovery and reconstruction of central nervous system function after spinal cord injury(SCI)is a worldwide problem.The difficulty lies in the feasibility issue of new axons passing through the injured area and the...The recovery and reconstruction of central nervous system function after spinal cord injury(SCI)is a worldwide problem.The difficulty lies in the feasibility issue of new axons passing through the injured area and the negative effect of scarring after injury.As a biological material,the human amniotic membrane(HAM)has the advantages of protecting nerve growth,inhibiting scar formation,and promoting neovascularization,but its weak physical properties are difficult to apply in treating SCI.In this study,HAMs were first decellularized and then chemically grafted with methacrylic anhydride.Next,the composite was photocrosslinked with gelatin methacrylate to prepare a cross-network biological complex.The final complexes prepared by appeal were used for in vitro and in vivo studies of SCI in rats,separately.In the in vitro experiment,the composite scaffold inherited abundant biological factors from the amniotic membrane and had the physical properties of a hydrogel,thus providing a favorable environment for the growth and development of neurons and blood vessels.In the in vivo experiment,the composite reduced scarring and promoted the growth of new nerves.Overall,the composite scaffolds can stably simulate the extracellular microenvironment in SCI defects,regulate pathological changes,and promote the generation of new neurons.Therefore,decellularized HAM hydrogels are promising biocomposite materials for central nerve repair after SCI.展开更多
In this paper, the problem of abnormal spectrum usage between satellite spectrum sharing systems is investigated to support multi-satellite spectrum coexistence. Given the cost of monitoring, the mobility of low-orbit...In this paper, the problem of abnormal spectrum usage between satellite spectrum sharing systems is investigated to support multi-satellite spectrum coexistence. Given the cost of monitoring, the mobility of low-orbit satellites, and the directional nature of their signals, traditional monitoring methods are no longer suitable, especially in the case of multiple power level. Mobile crowdsensing(MCS), as a new technology, can make full use of idle resources to complete a variety of perceptual tasks. However, traditional MCS heavily relies on a centralized server and is vulnerable to single point of failure attacks. Therefore, we replace the original centralized server with a blockchain-based distributed service provider to enable its security. Therefore, in this work, we propose a blockchain-based MCS framework, in which we explain in detail how this framework can achieve abnormal frequency behavior monitoring in an inter-satellite spectrum sharing system. Then, under certain false alarm probability, we propose an abnormal spectrum detection algorithm based on mixed hypothesis test to maximize detection probability in single power level and multiple power level scenarios, respectively. Finally, a Bad out of Good(BooG) detector is proposed to ease the computational pressure on the blockchain nodes. Simulation results show the effectiveness of the proposed framework.展开更多
●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHODS:Barrier models of HCEs were tre...●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHODS:Barrier models of HCEs were treated with recombinant human Sema7A at concentrations of 0,125,250,or 500 ng/mL for 24,48,or 72h in vitro.Transepithelial electrical resistance(TEER)as well as Dextran-fluorescein isothiocyanate(FITC)permeability assays were conducted to assess barrier function.To quantify tight junctions(TJs)such as occludin and zonula occludens-1(ZO-1)at the mRNA level,reverse transcriptionpolymerase chain reaction(RT-PCR)analysis was performed.Immunoblotting was used to examine the activity of the nuclear factor-kappa B(NF-κB)signaling pathway and the production of TJs proteins.Immunofluorescence analyses were employed to localize the TJs.Enzyme-linked immunosorbent assay(ELISA)and RT-PCR were utilized to observe changes in interleukin(IL)-1βlevels.To investigate the role of NF-κB signaling activation and IL^(-1)βin Sema7A’s anti-barrier mechanism,we employed 0.1μmol/L IκB kinase 2(IKK2)inhibitor IV or 500 ng/mL IL^(-1)receptor(IL-1R)antagonist.●RESULTS:Treatment with Sema7A resulted in decreased TEER and increased permeability of Dextran-FITC in HCEs through down-regulating mRNA and protein levels of TJs in a time-and dose-dependent manner,as well as altering the localization of TJs.Furthermore,Sema7A stimulated the activation of inhibitor of kappa B alpha(IκBα)and expression of IL-1β.The anti-barrier function of Sema7A was significantly suppressed by treatment with IKK2 inhibitor IV or IL-1R antagonists.●CONCLUSION:Sema7A disrupts barrier function through its influence on NF-κB-mediated expression of TJ proteins,as well as the expression of IL-1β.These findings suggest that Sema7A could be a potential therapeutic target for the diseases in corneal epithelium.展开更多
Optical mode converters are essential for enhancing the capacity of optical communication systems. However, fabrication errors restrict the further improvement of conventional mode converters. To address this challeng...Optical mode converters are essential for enhancing the capacity of optical communication systems. However, fabrication errors restrict the further improvement of conventional mode converters. To address this challenge, we have designed an on-chip TE0–TE1mode converter based on topologically protected waveguide arrays. The simulation results demonstrate that the converter exhibits a mode coupling efficiency of 93.5% near 1550 nm and can tolerate a relative fabrication error of 30%. Our design approach can be extended to enhance the robustness for other integrated photonic devices, beneficial for future development of optical network systems.展开更多
Proton-activated G protein-coupled receptors(GPCRs),initially discovered by Ludwig in 2003,are widely distributed in various tissues.These receptors have been found to modulate the immune system in several inflammator...Proton-activated G protein-coupled receptors(GPCRs),initially discovered by Ludwig in 2003,are widely distributed in various tissues.These receptors have been found to modulate the immune system in several inflammatory diseases,including inflammatory bowel disease,atopic dermatitis,and asthma.Proton-activated GPCRs belong to the G protein-coupled receptor family and can detect alternations in extracellular pH.This detection triggers downstream signaling pathways within the cells,ultimately influencing the function of immune cells.In this review,we specifically focused on investigating the immune response of proton-activated GPCRs under inflammatory conditions.展开更多
BACKGROUND Mesenchymal stem cells(MSCs)are capable of self-renewal and differentiation,and extensive studies have demonstrated their therapeutic potential in atherosclerosis(AS).AIM To conduct a bibliometric analysis ...BACKGROUND Mesenchymal stem cells(MSCs)are capable of self-renewal and differentiation,and extensive studies have demonstrated their therapeutic potential in atherosclerosis(AS).AIM To conduct a bibliometric analysis of studies on the use of MSC therapy for AS over the past two decades,assess key trends and provide insights for future research directions.METHODS We systematically searched the Web of Science Core Collection database for articles published between 1999 and 2023,yielding a total of 556 articles.Visual representation and bibliometric analysis of information and trends were facilitated using CiteSpace,the R package‘bibliometrix’and VOSviewer.RESULTS The analyzed articles were predominantly from 52 countries/regions,with prominent contributions from China and the United States.A cohort of 3057 authors contributed to these publications,with the works of Libby P distinguished by their influence and citation count.Int J Mol Sci has emerged as the journal with the highest publication volume,prominently disseminating influential papers and identifying citation outbreaks.Furthermore,our analysis identified current research hotspots within the field,focusing on vascular progenitor cells,inflammatory mechanisms,and extracellular vesicles.Emerging research frontiers,such as extracellular vesicles and oxidative stress,have been highlighted as areas of burgeoning interest.Finally,we offer perspectives on the status of research and future directions of MSC therapy in AS.CONCLUSION This comprehensive analysis provides valuable insights for advancing scientific research on MSC therapy for AS.By elucidating pivotal trends and research directions,this study aimed to foster innovation and promote the progress of disciplines in this field,thereby contributing to advancing scientific knowledge and clinical practice.展开更多
Real-time acquisition of human pulse signals in daily life is clinically important for cardiovascular disease monitoring and diagnosis.Here,we propose a smart photonic wristband for pulse signal monitoring based on sp...Real-time acquisition of human pulse signals in daily life is clinically important for cardiovascular disease monitoring and diagnosis.Here,we propose a smart photonic wristband for pulse signal monitoring based on speckle pattern analysis with a polymer optical fiber(POF)integrated into a sports wristband.Several different speckle pattern processing algorithms and POFs with different core diameters were evaluated.The results indicated that the smart photonic wristband had a high signal-to-noise ratio and low latency,with the measurement error controlled at approximately 3.7%.This optimized pulse signal could be used for further medical diagnosis and was capable of objectively monitoring subtle pulse signal changes,such as the pulse waveform at different positions of Cunkou and pulse waveforms before and after exercise.With the assistance of artificial intelligence(AI),functions such as gesture recognition have been realized through the established prediction model by processing pulse signals,in which the recognition accuracy reaches 95%.Our AI-assisted smart photonic wristband has potential applications for clinical treatment of cardiovascular diseases and home monitoring,paving the way for medical Internet of Things-enabled smart systems.展开更多
The shot put is a sport that requires very high explosive power and precise technique.Strength training occupies the core position in the training of shot-putters,which can not only improve the throwing distance of at...The shot put is a sport that requires very high explosive power and precise technique.Strength training occupies the core position in the training of shot-putters,which can not only improve the throwing distance of athletes but also enhance their competitive state and prevent sports injuries.The purpose of this paper is to analyze the principles,classification,methods,and specific exercises of strength training in the training of shot-putters,in order to provide scientific training guidance for shot-putters.展开更多
In this study,an acid-induced assembly strategy for a rutile TiO2 photocatalyst was proposed on the basis of the treatment of lamellar protonated titanate with a concentrated HNO3 solution.Nitrate groups were successf...In this study,an acid-induced assembly strategy for a rutile TiO2 photocatalyst was proposed on the basis of the treatment of lamellar protonated titanate with a concentrated HNO3 solution.Nitrate groups were successfully grafted onto a TiO2 surface and induced the assembly of rutile TiO2 nanorods into uniform spindle-like nanobundles.The resulting TiO2 product achieved a photocatalytic hydrogen evolution rate of 402.4μmol h^?1,which is 3.1 times higher than that of Degussa P25-TiO2.It was demonstrated that nitrate group grafting caused the rutile TiO2 surface to become negatively charged,which is favorable for trapping positive protons and improving charge carrier separation,thereby enhancing photocatalytic hydrogen production.Additionally,surface charges were crucial to structural stability based on electrostatic repulsion.This study not only developed a facile surface modification strategy for fabricating efficient H2 production photocatalysts but also identified an influence mechanism of inorganic acids different from that reported in the literature.展开更多
Objective To investigate the influence of vitamin D receptor （VDR） gene polymorphisms on susceptibility to type 1 diabetes mellitus （T1DM） in the Chinese Han population. Method One hundred and thirty-six Chinese ...Objective To investigate the influence of vitamin D receptor （VDR） gene polymorphisms on susceptibility to type 1 diabetes mellitus （T1DM） in the Chinese Han population. Method One hundred and thirty-six Chinese Han people, including 54 T1DM patients and 82 unrelated healthy subjects as control were genotyped by polymerase chain reaction-restriction fragment length polymorphism for three restriction sites in the VDR gene, which were ApaI, TaqI, and BamL Results The frequency of B allele of BsmI site in VDR gene was significantly higher in T1DM patients than in healthy subjects （ P = 0. 033 ） while no difference was found between the two groups in the distribution of ApaI and TaqI polymorphisms. Conclusion The BsmI polymorphism of VDR gene may be associated with the susceptibihty to T1DM in the Chinese Han population of Beijing.展开更多
Command, control, communication, computing, intel- ligence, surveillance and reconnaissance （C^4ISR） in information age is a complex system whose structure always changes ac- tively or passively during the warfare. ...Command, control, communication, computing, intel- ligence, surveillance and reconnaissance （C^4ISR） in information age is a complex system whose structure always changes ac- tively or passively during the warfare. Therefore, it is important to optimize the structure, especially in ambiguous and quick-tempo modern warfare. This paper proposes an adaptive evolvement mechanism for the C^4ISR structure to survive the changeable warfare. Firstly, the information age C^4ISR structure is defined and modeled based on the complex network theory. Secondly, taking the observe, orient, decide and act （OODA） model into consideration, four kinds of loops in the C^4ISR structure are pro- posed and their coefficient of networked effects （CNE） is further defined. Then, the adaptive evolvement mechanisms of the four kinds of loops are presented respectively. Finally, taking the joint air-defense C^4ISR as an example, simulation experiments are im- plemented, which validate the evolvement mechanism and show that the information age C41SR structure has some characteristics of small-world network and scale-free network.展开更多
The chrysanthemum genome harbors three FT-like genes:CmFTL1 and CmFTL3 are thought to act as regulators of floral induction under long-day(LD)and short-day(SD)conditions,respectively,whereas the function of CmFTL2 is ...The chrysanthemum genome harbors three FT-like genes:CmFTL1 and CmFTL3 are thought to act as regulators of floral induction under long-day(LD)and short-day(SD)conditions,respectively,whereas the function of CmFTL2 is currently unclear.The objective of the present research was to explore the function of CmFTL2 in the determination of flowering time of the photo-insensitive chrysanthemum cultivar‘Floral Yuuka’,both in response to variation in the photoperiod and to the exogenous provision of sucrose.Spraying leaves of‘Floral Yuuka’plants with 50 mM sucrose accelerated flowering and increased the level of CmFTL2 transcription in the leaf more strongly than either CmFTL1 or FTL3 under both long and SD conditions.Transcription profiling indicated that all three CmFTL genes were upregulated during floral induction.The relationship of the CmFTL2 sequence with that of other members of the PEBP family suggested that its product contributes to the florigen rather than to the anti-florigen complex.The heterologous expression of CmFTL2 in the Arabidopsis thaliana ft-10 mutant rescued the mutant phenotype,showing that CmFTL2 could compensate for the absence of FT.These results suggest that CmFTL2 acts as a regulator of floral transition and responds to both the photoperiod and sucrose.展开更多
AIM: To study the role of luteolin(LUT) in the expression of toll-like receptors 3(TLR3) ligand poly I:C stimulated inflammatory factors in human corneal fibroblasts(HCFs).METHODS: HCFs cells were cultivated with or w...AIM: To study the role of luteolin(LUT) in the expression of toll-like receptors 3(TLR3) ligand poly I:C stimulated inflammatory factors in human corneal fibroblasts(HCFs).METHODS: HCFs cells were cultivated with or without LUT or poly I:C.The expression levels of interleukin(IL)-6, IL-8, monocyte chemotactic protein-1(MCP-1), vascular cell adhesion molecule(VCAM)-1, as well as intercellular adhesion molecule(ICAM)-1 were measured using enzymelinked immunosorbent assay(ELISA), immunoblotting or reverse transcription-quantitative polymerase chain reaction(PCR) analyses.Immunoblotting was used to assess toll-interleukin-1 receptor-domain-containing adapter-inducing interferon-β(TRIF), TLR3, transforming growth factor-bactivated kinase 1(TAK1), tumor necrosis factor receptor-associated factor 6(TRAF6), the transcription factor AP-1, as well as transcription factor nuclear factor(NF-κB)–inhibitory protein IκB-α degradation and phosphorylation.Immunofluorescence assays were used to localize the cellular location of the p65 subunit of NF-κB.RESULTS: Corneal fibroblasts exposed to poly I:C demonstrated decreased VCAM-1, ICAM-1, MCP-1, IL-6, and IL-8 expression levels upon exposure to LUT in a time-dependent and concentration-dependent manner.LUT was observed to suppress poly I:C-triggered expression of TLR3, the translocation of NF-κB p65 into cell nuclei, as well as the phosphorylation of TAK, c-Jun, and IκB-α, while no impact on the expression levels of TRIF and TRAF6 were observed.CONCLUSION: LUT suppress the expression of proinflammatory adhesion molecules, chemokines, and cytokines in poly I:C exposed HCFs.These effects are likely mediated through TAK/NF-κB signal attenuation.Therefore, LUT is a candidate molecule that can prevent the TLR3-mediated inflammation response associated with corneal viral infection.展开更多
Salamanders are unique among tetrapods in their ability to regenerate the limbs throughout life.Like other poikilothermic amphibians,salamanders also show a remarkable capacity to survive long periods of starvation.Wh...Salamanders are unique among tetrapods in their ability to regenerate the limbs throughout life.Like other poikilothermic amphibians,salamanders also show a remarkable capacity to survive long periods of starvation.Whether the physiological reserves necessary for tissue regeneration are preserved or sacrificed in starved salamanders is unknown.In the current study,we maintained Iberian ribbed newts(Pleurodeles waltl)under extreme physiological stress to assess the extent of regeneration and identify the molecular and cellular changes that may occur under such conditions.After 19 months of complete food deprivation,the animals exhibited extensive morphological and physiological adaptations but remained behaviorally active and vigilant.Autophagy was elevated in different tissues and the transformed gut microbiota indicated remodeling of the intestinal tract related to autophagy.Upon limb amputation in animals starved for 21 months,regeneration proceeded with progenitor cell proliferation and migration,leading to limb blastema formation.However,limb outgrowth and patterning were substantially attenuated.Blockage of autophagy inhibited cell proliferation and blastema formation in starved animals,but not in fed animals.Hence,tissue autophagy and the regenerative response were tightly coupled only when animals were under stress.Our results demonstrate that under adverse conditions,salamanders can exploit alternative strategies to secure blastema formation for limb regeneration.展开更多
Natural and human-induced changes may exert considerable impacts on the seasonal and nodal dynamics of M2 and K1 tidal constituents.Therefore,quantifying the influences of these factors on tidal regime changes is esse...Natural and human-induced changes may exert considerable impacts on the seasonal and nodal dynamics of M2 and K1 tidal constituents.Therefore,quantifying the influences of these factors on tidal regime changes is essential for sustainable water resources management in coastal environments.In this study,the enhanced harmonic analysis was applied to extract the seasonal variability of the M2 and K1 tidal amplitudes and phases at three gauging stations along Lingdingyang Bay of the Zhujiang River Delta.The seasonal dynamics in terms of tidal wave celerity and amplification/damping rate were used to quantify the impacts of human-induced estuarine morphological alterations on M2 and K1 tidal hydrodynamics in inner and outer Lingdingyang Bay.The results show that both tidal amplification/damping rate and wave celerity were considerably increased from the pre-anthropogenic activity period(Pre-AAP)to the post-anthropogenic activity period(Post-AAP)excepting the tidal amplification/damping rate in outer Lingdingyang Bay,and the variations in outer Lingdingyang Bay was larger than those in inner Lingdingyang Bay.The alterations in these two parameters were more significant in flood season than in dry season in both inner and outer Lingdingyang Bay.The seasonal variability of M2 and K1 tidal amplitudes were further quantified using a regression model accounting for the 18.61-year lunar nodal modulation,where this study observes a considerable alteration in M2 constituent owing to human interventions.During the Post-AAP,the M2 amplitudes at the downstream station were larger than those that would have occurred in the absence of strong human interventions,whereas the opposite was true for the upstream station,leading to a substantial decrease in tidal amplification in outer Lingdingyang Bay.However,it is opposite in inner Lingdingyang Bay.The underlying mechanism can be primarily attributed to channel deepening and narrowing caused by human interventions,that resulted in substantial enlargement of the bay volume and reduced the effective bottom friction,leading to faster wave celerity and stronger amplified waves.展开更多
基金supported by the National Natural Science Foundation of China(No.82371383)。
文摘The recovery and reconstruction of central nervous system function after spinal cord injury(SCI)is a worldwide problem.The difficulty lies in the feasibility issue of new axons passing through the injured area and the negative effect of scarring after injury.As a biological material,the human amniotic membrane(HAM)has the advantages of protecting nerve growth,inhibiting scar formation,and promoting neovascularization,but its weak physical properties are difficult to apply in treating SCI.In this study,HAMs were first decellularized and then chemically grafted with methacrylic anhydride.Next,the composite was photocrosslinked with gelatin methacrylate to prepare a cross-network biological complex.The final complexes prepared by appeal were used for in vitro and in vivo studies of SCI in rats,separately.In the in vitro experiment,the composite scaffold inherited abundant biological factors from the amniotic membrane and had the physical properties of a hydrogel,thus providing a favorable environment for the growth and development of neurons and blood vessels.In the in vivo experiment,the composite reduced scarring and promoted the growth of new nerves.Overall,the composite scaffolds can stably simulate the extracellular microenvironment in SCI defects,regulate pathological changes,and promote the generation of new neurons.Therefore,decellularized HAM hydrogels are promising biocomposite materials for central nerve repair after SCI.
文摘In this paper, the problem of abnormal spectrum usage between satellite spectrum sharing systems is investigated to support multi-satellite spectrum coexistence. Given the cost of monitoring, the mobility of low-orbit satellites, and the directional nature of their signals, traditional monitoring methods are no longer suitable, especially in the case of multiple power level. Mobile crowdsensing(MCS), as a new technology, can make full use of idle resources to complete a variety of perceptual tasks. However, traditional MCS heavily relies on a centralized server and is vulnerable to single point of failure attacks. Therefore, we replace the original centralized server with a blockchain-based distributed service provider to enable its security. Therefore, in this work, we propose a blockchain-based MCS framework, in which we explain in detail how this framework can achieve abnormal frequency behavior monitoring in an inter-satellite spectrum sharing system. Then, under certain false alarm probability, we propose an abnormal spectrum detection algorithm based on mixed hypothesis test to maximize detection probability in single power level and multiple power level scenarios, respectively. Finally, a Bad out of Good(BooG) detector is proposed to ease the computational pressure on the blockchain nodes. Simulation results show the effectiveness of the proposed framework.
基金Supported by the National Natural Science Foundation of China(No.81770889)Zhuhai Science and Technology Program(No.ZH22036201210134PWC).
文摘●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHODS:Barrier models of HCEs were treated with recombinant human Sema7A at concentrations of 0,125,250,or 500 ng/mL for 24,48,or 72h in vitro.Transepithelial electrical resistance(TEER)as well as Dextran-fluorescein isothiocyanate(FITC)permeability assays were conducted to assess barrier function.To quantify tight junctions(TJs)such as occludin and zonula occludens-1(ZO-1)at the mRNA level,reverse transcriptionpolymerase chain reaction(RT-PCR)analysis was performed.Immunoblotting was used to examine the activity of the nuclear factor-kappa B(NF-κB)signaling pathway and the production of TJs proteins.Immunofluorescence analyses were employed to localize the TJs.Enzyme-linked immunosorbent assay(ELISA)and RT-PCR were utilized to observe changes in interleukin(IL)-1βlevels.To investigate the role of NF-κB signaling activation and IL^(-1)βin Sema7A’s anti-barrier mechanism,we employed 0.1μmol/L IκB kinase 2(IKK2)inhibitor IV or 500 ng/mL IL^(-1)receptor(IL-1R)antagonist.●RESULTS:Treatment with Sema7A resulted in decreased TEER and increased permeability of Dextran-FITC in HCEs through down-regulating mRNA and protein levels of TJs in a time-and dose-dependent manner,as well as altering the localization of TJs.Furthermore,Sema7A stimulated the activation of inhibitor of kappa B alpha(IκBα)and expression of IL-1β.The anti-barrier function of Sema7A was significantly suppressed by treatment with IKK2 inhibitor IV or IL-1R antagonists.●CONCLUSION:Sema7A disrupts barrier function through its influence on NF-κB-mediated expression of TJ proteins,as well as the expression of IL-1β.These findings suggest that Sema7A could be a potential therapeutic target for the diseases in corneal epithelium.
基金Project supported by the National Undergraduate Training Projects for Innovation and Entrepreneurship (Grant No. 5003182007)the National Natural Science Foundation of China (Grant No. 12074137)+1 种基金the National Key Research and Development Project of China (Grant No. 2021YFB2801903)the Natural Science Foundation from the Science,Technology,and Innovation Commission of Shenzhen Municipality (Grant No. JCYJ20220530161010023)。
文摘Optical mode converters are essential for enhancing the capacity of optical communication systems. However, fabrication errors restrict the further improvement of conventional mode converters. To address this challenge, we have designed an on-chip TE0–TE1mode converter based on topologically protected waveguide arrays. The simulation results demonstrate that the converter exhibits a mode coupling efficiency of 93.5% near 1550 nm and can tolerate a relative fabrication error of 30%. Our design approach can be extended to enhance the robustness for other integrated photonic devices, beneficial for future development of optical network systems.
基金supported by the National Nature Science Foundation of China(No.81873694)the Key Research and Development Program of Hubei Province(No.2022BCA005)Knowledge Innovation Program of Wuhan Basic Research(No.2022020801010446).
文摘Proton-activated G protein-coupled receptors(GPCRs),initially discovered by Ludwig in 2003,are widely distributed in various tissues.These receptors have been found to modulate the immune system in several inflammatory diseases,including inflammatory bowel disease,atopic dermatitis,and asthma.Proton-activated GPCRs belong to the G protein-coupled receptor family and can detect alternations in extracellular pH.This detection triggers downstream signaling pathways within the cells,ultimately influencing the function of immune cells.In this review,we specifically focused on investigating the immune response of proton-activated GPCRs under inflammatory conditions.
基金Supported by the Regional Cooperation Program of Shanxi Province,China,No.202204041101038the Construction and Demonstration of the Molecular Diagnosis and Treatment Platform for Vascular Diseases in Shanxi Province,China,No.SCP-2023-17Translational Medicine Engineering Research Center for Vascular Diseases of Shanxi Province,China,No.2022017.
文摘BACKGROUND Mesenchymal stem cells(MSCs)are capable of self-renewal and differentiation,and extensive studies have demonstrated their therapeutic potential in atherosclerosis(AS).AIM To conduct a bibliometric analysis of studies on the use of MSC therapy for AS over the past two decades,assess key trends and provide insights for future research directions.METHODS We systematically searched the Web of Science Core Collection database for articles published between 1999 and 2023,yielding a total of 556 articles.Visual representation and bibliometric analysis of information and trends were facilitated using CiteSpace,the R package‘bibliometrix’and VOSviewer.RESULTS The analyzed articles were predominantly from 52 countries/regions,with prominent contributions from China and the United States.A cohort of 3057 authors contributed to these publications,with the works of Libby P distinguished by their influence and citation count.Int J Mol Sci has emerged as the journal with the highest publication volume,prominently disseminating influential papers and identifying citation outbreaks.Furthermore,our analysis identified current research hotspots within the field,focusing on vascular progenitor cells,inflammatory mechanisms,and extracellular vesicles.Emerging research frontiers,such as extracellular vesicles and oxidative stress,have been highlighted as areas of burgeoning interest.Finally,we offer perspectives on the status of research and future directions of MSC therapy in AS.CONCLUSION This comprehensive analysis provides valuable insights for advancing scientific research on MSC therapy for AS.By elucidating pivotal trends and research directions,this study aimed to foster innovation and promote the progress of disciplines in this field,thereby contributing to advancing scientific knowledge and clinical practice.
基金financial supports from National Key R&D Program of China (2022YFE0140400)National Natural Science Foundation of China(62003046, 62111530238)+7 种基金Guangdong Basic and Applied Basic Research Foundation (2021A1515011997)The Supplemental Funds for Major Scientific Research Projects of Beijing Normal University,Zhuhai(ZHPT2023007)Special project in key field of Guangdong Provincial Department of Education (2021ZDZX1050)The Innovation Team Project of Guangdong Provincial Department of Education (2021KCXTD014)Fundação para a Ciência e a Tecnologia (FCT) through the 2021.00667CEECIND (iAqua project)PTDC/EEI-EEE/0415/2021 (DigiAqua project)The project i3N,UIDB/50025/2020 n&UIDP/50025/2020, financed by national funds through the FCT/MEC
文摘Real-time acquisition of human pulse signals in daily life is clinically important for cardiovascular disease monitoring and diagnosis.Here,we propose a smart photonic wristband for pulse signal monitoring based on speckle pattern analysis with a polymer optical fiber(POF)integrated into a sports wristband.Several different speckle pattern processing algorithms and POFs with different core diameters were evaluated.The results indicated that the smart photonic wristband had a high signal-to-noise ratio and low latency,with the measurement error controlled at approximately 3.7%.This optimized pulse signal could be used for further medical diagnosis and was capable of objectively monitoring subtle pulse signal changes,such as the pulse waveform at different positions of Cunkou and pulse waveforms before and after exercise.With the assistance of artificial intelligence(AI),functions such as gesture recognition have been realized through the established prediction model by processing pulse signals,in which the recognition accuracy reaches 95%.Our AI-assisted smart photonic wristband has potential applications for clinical treatment of cardiovascular diseases and home monitoring,paving the way for medical Internet of Things-enabled smart systems.
文摘The shot put is a sport that requires very high explosive power and precise technique.Strength training occupies the core position in the training of shot-putters,which can not only improve the throwing distance of athletes but also enhance their competitive state and prevent sports injuries.The purpose of this paper is to analyze the principles,classification,methods,and specific exercises of strength training in the training of shot-putters,in order to provide scientific training guidance for shot-putters.
基金supported by the National Natural Science Foundation of China (21771070, 21571071)the Fundamental Research Funds for the Central Universities (2018KFYYXJJ120, 2019KFYRCPY104)~~
文摘In this study,an acid-induced assembly strategy for a rutile TiO2 photocatalyst was proposed on the basis of the treatment of lamellar protonated titanate with a concentrated HNO3 solution.Nitrate groups were successfully grafted onto a TiO2 surface and induced the assembly of rutile TiO2 nanorods into uniform spindle-like nanobundles.The resulting TiO2 product achieved a photocatalytic hydrogen evolution rate of 402.4μmol h^?1,which is 3.1 times higher than that of Degussa P25-TiO2.It was demonstrated that nitrate group grafting caused the rutile TiO2 surface to become negatively charged,which is favorable for trapping positive protons and improving charge carrier separation,thereby enhancing photocatalytic hydrogen production.Additionally,surface charges were crucial to structural stability based on electrostatic repulsion.This study not only developed a facile surface modification strategy for fabricating efficient H2 production photocatalysts but also identified an influence mechanism of inorganic acids different from that reported in the literature.
文摘Objective To investigate the influence of vitamin D receptor （VDR） gene polymorphisms on susceptibility to type 1 diabetes mellitus （T1DM） in the Chinese Han population. Method One hundred and thirty-six Chinese Han people, including 54 T1DM patients and 82 unrelated healthy subjects as control were genotyped by polymerase chain reaction-restriction fragment length polymorphism for three restriction sites in the VDR gene, which were ApaI, TaqI, and BamL Results The frequency of B allele of BsmI site in VDR gene was significantly higher in T1DM patients than in healthy subjects （ P = 0. 033 ） while no difference was found between the two groups in the distribution of ApaI and TaqI polymorphisms. Conclusion The BsmI polymorphism of VDR gene may be associated with the susceptibihty to T1DM in the Chinese Han population of Beijing.
基金supported by the National Defense Basic Research Program of China and National Defense Pre-Research Foundation of China
文摘Command, control, communication, computing, intel- ligence, surveillance and reconnaissance （C^4ISR） in information age is a complex system whose structure always changes ac- tively or passively during the warfare. Therefore, it is important to optimize the structure, especially in ambiguous and quick-tempo modern warfare. This paper proposes an adaptive evolvement mechanism for the C^4ISR structure to survive the changeable warfare. Firstly, the information age C^4ISR structure is defined and modeled based on the complex network theory. Secondly, taking the observe, orient, decide and act （OODA） model into consideration, four kinds of loops in the C^4ISR structure are pro- posed and their coefficient of networked effects （CNE） is further defined. Then, the adaptive evolvement mechanisms of the four kinds of loops are presented respectively. Finally, taking the joint air-defense C^4ISR as an example, simulation experiments are im- plemented, which validate the evolvement mechanism and show that the information age C41SR structure has some characteristics of small-world network and scale-free network.
基金This work was supported by funding from the National Natural Science Foundation of China(31372100)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutionsby‘Programs of Innovation and Entrepreneurship Talents’of Jiangsu Province.
文摘The chrysanthemum genome harbors three FT-like genes:CmFTL1 and CmFTL3 are thought to act as regulators of floral induction under long-day(LD)and short-day(SD)conditions,respectively,whereas the function of CmFTL2 is currently unclear.The objective of the present research was to explore the function of CmFTL2 in the determination of flowering time of the photo-insensitive chrysanthemum cultivar‘Floral Yuuka’,both in response to variation in the photoperiod and to the exogenous provision of sucrose.Spraying leaves of‘Floral Yuuka’plants with 50 mM sucrose accelerated flowering and increased the level of CmFTL2 transcription in the leaf more strongly than either CmFTL1 or FTL3 under both long and SD conditions.Transcription profiling indicated that all three CmFTL genes were upregulated during floral induction.The relationship of the CmFTL2 sequence with that of other members of the PEBP family suggested that its product contributes to the florigen rather than to the anti-florigen complex.The heterologous expression of CmFTL2 in the Arabidopsis thaliana ft-10 mutant rescued the mutant phenotype,showing that CmFTL2 could compensate for the absence of FT.These results suggest that CmFTL2 acts as a regulator of floral transition and responds to both the photoperiod and sucrose.
基金Supported by the National Natural Science Foundation of China (No.81770889)Administration of Traditional Chinese Medicine of Guangdong Province (No.20201070)。
文摘AIM: To study the role of luteolin(LUT) in the expression of toll-like receptors 3(TLR3) ligand poly I:C stimulated inflammatory factors in human corneal fibroblasts(HCFs).METHODS: HCFs cells were cultivated with or without LUT or poly I:C.The expression levels of interleukin(IL)-6, IL-8, monocyte chemotactic protein-1(MCP-1), vascular cell adhesion molecule(VCAM)-1, as well as intercellular adhesion molecule(ICAM)-1 were measured using enzymelinked immunosorbent assay(ELISA), immunoblotting or reverse transcription-quantitative polymerase chain reaction(PCR) analyses.Immunoblotting was used to assess toll-interleukin-1 receptor-domain-containing adapter-inducing interferon-β(TRIF), TLR3, transforming growth factor-bactivated kinase 1(TAK1), tumor necrosis factor receptor-associated factor 6(TRAF6), the transcription factor AP-1, as well as transcription factor nuclear factor(NF-κB)–inhibitory protein IκB-α degradation and phosphorylation.Immunofluorescence assays were used to localize the cellular location of the p65 subunit of NF-κB.RESULTS: Corneal fibroblasts exposed to poly I:C demonstrated decreased VCAM-1, ICAM-1, MCP-1, IL-6, and IL-8 expression levels upon exposure to LUT in a time-dependent and concentration-dependent manner.LUT was observed to suppress poly I:C-triggered expression of TLR3, the translocation of NF-κB p65 into cell nuclei, as well as the phosphorylation of TAK, c-Jun, and IκB-α, while no impact on the expression levels of TRIF and TRAF6 were observed.CONCLUSION: LUT suppress the expression of proinflammatory adhesion molecules, chemokines, and cytokines in poly I:C exposed HCFs.These effects are likely mediated through TAK/NF-κB signal attenuation.Therefore, LUT is a candidate molecule that can prevent the TLR3-mediated inflammation response associated with corneal viral infection.
基金This study was supported by the National Natural Science Foundation of China(31771617)and HZAU-AGIS Cooperation Fund(SZYJY2021009)。
文摘Salamanders are unique among tetrapods in their ability to regenerate the limbs throughout life.Like other poikilothermic amphibians,salamanders also show a remarkable capacity to survive long periods of starvation.Whether the physiological reserves necessary for tissue regeneration are preserved or sacrificed in starved salamanders is unknown.In the current study,we maintained Iberian ribbed newts(Pleurodeles waltl)under extreme physiological stress to assess the extent of regeneration and identify the molecular and cellular changes that may occur under such conditions.After 19 months of complete food deprivation,the animals exhibited extensive morphological and physiological adaptations but remained behaviorally active and vigilant.Autophagy was elevated in different tissues and the transformed gut microbiota indicated remodeling of the intestinal tract related to autophagy.Upon limb amputation in animals starved for 21 months,regeneration proceeded with progenitor cell proliferation and migration,leading to limb blastema formation.However,limb outgrowth and patterning were substantially attenuated.Blockage of autophagy inhibited cell proliferation and blastema formation in starved animals,but not in fed animals.Hence,tissue autophagy and the regenerative response were tightly coupled only when animals were under stress.Our results demonstrate that under adverse conditions,salamanders can exploit alternative strategies to secure blastema formation for limb regeneration.
基金The National Key R&D Program of China under contract No.2016YFC0402600the National Natural Science Foundation of China under contract No.51979296the Guangzhou Science and Technology Program of China under contract No.202002030452。
文摘Natural and human-induced changes may exert considerable impacts on the seasonal and nodal dynamics of M2 and K1 tidal constituents.Therefore,quantifying the influences of these factors on tidal regime changes is essential for sustainable water resources management in coastal environments.In this study,the enhanced harmonic analysis was applied to extract the seasonal variability of the M2 and K1 tidal amplitudes and phases at three gauging stations along Lingdingyang Bay of the Zhujiang River Delta.The seasonal dynamics in terms of tidal wave celerity and amplification/damping rate were used to quantify the impacts of human-induced estuarine morphological alterations on M2 and K1 tidal hydrodynamics in inner and outer Lingdingyang Bay.The results show that both tidal amplification/damping rate and wave celerity were considerably increased from the pre-anthropogenic activity period(Pre-AAP)to the post-anthropogenic activity period(Post-AAP)excepting the tidal amplification/damping rate in outer Lingdingyang Bay,and the variations in outer Lingdingyang Bay was larger than those in inner Lingdingyang Bay.The alterations in these two parameters were more significant in flood season than in dry season in both inner and outer Lingdingyang Bay.The seasonal variability of M2 and K1 tidal amplitudes were further quantified using a regression model accounting for the 18.61-year lunar nodal modulation,where this study observes a considerable alteration in M2 constituent owing to human interventions.During the Post-AAP,the M2 amplitudes at the downstream station were larger than those that would have occurred in the absence of strong human interventions,whereas the opposite was true for the upstream station,leading to a substantial decrease in tidal amplification in outer Lingdingyang Bay.However,it is opposite in inner Lingdingyang Bay.The underlying mechanism can be primarily attributed to channel deepening and narrowing caused by human interventions,that resulted in substantial enlargement of the bay volume and reduced the effective bottom friction,leading to faster wave celerity and stronger amplified waves.
