The new challenges that the phenomenon of demographic aging imposes on society in general, and health services in particular, can impact active and healthy aging. The project was implemented for two years （2012/2013...The new challenges that the phenomenon of demographic aging imposes on society in general, and health services in particular, can impact active and healthy aging. The project was implemented for two years （2012/2013） and aimed to combat loneliness in the elderly, which constitutes an obstacle to healthy and active aging. Decrease the sense of loneliness of individuals from 65 to 84 years of age, the project was made by using the health planning methodology, starting with a descriptive study （health diagnosis of the independent elderly population） followed by the development, implementation and evaluation of a community intervention project, which included home visits to the elderly, assessment of the level of loneliness in two different times （UCLA scale） and various intergenerational activities of social life （dancing, walking, online courses, games...） using a cross-section of partnerships and community involvement. There was a decrease from 62.8% to 41.9% in the reported overall feeling of loneliness of independent individuals between the ages of 65 and 84 years of age. There was an increase from 50.0% to 86.4% of seniors who now have dreams/life projects. The reduction of social and emotional isolation resulted in the promotion of social networks, which not only encouraged social interaction but also considered the elderly who live alone. The health gains arising from it are evidenced in contributing more proactively and the improvement of the quality of life in the elderly community.展开更多
The antibacterial potential of silver nanoparticles（AgNPs） resulted in their increasing incorporation into consumer,industrial and biomedical products.Therefore,human and environmental exposure to AgNPs（either as a...The antibacterial potential of silver nanoparticles（AgNPs） resulted in their increasing incorporation into consumer,industrial and biomedical products.Therefore,human and environmental exposure to AgNPs（either as an engineered product or a contaminant）supports the emergent research on the features conferring them different toxicity profiles.In this study,30 ran AgNPs coated with citrate or poly（ethylene glycol）（PEG） were used to assess the influence of coating on the effects produced on a human hepatoma cell line（HepG2）,namely in terms of viability,apoptosis,apoptotic related genes,cell cycle and cyclins gene expression.Both types of coated AgNPs decreased cell proliferation and viability with a similar toxicity profile.At the concentrations used（11 and 5 μg/mL corresponding to IC50 and-IC10 levels,respectively） the amount of cells undergoing apoptosis was not significant and the apoptotic related genes BCL2（anti-apoptotic gene）and BAX（pro-apoptotic gene） were both downregulated.Moreover,both AgNPs affected HepG2 cell cycle progression at the higher concentration（11 μg/mL） by increasing the percentage of cells in S（synthesis phase） and G2（Gap 2 phase） phases.Considering the cell-cycle related genes,the expression of cyclin B1 and cyclin E1 genes were decreased.Thus,this work has shown that citrate- and PEG-coated AgNPs impact on HepG2 apoptotic gene expression,cell cycle dynamics and cyclin regulation in a similar way.More research is needed to determine the properties that confer AgNPs at lower toxicity,since their use has proved helpful in several industrial and biomedical contexts.展开更多
Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their a...Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their ability to induce ecotoxic,cytotoxic,and mutagenic re-sponses in unicellular organisms and a human cell line.Ecotoxicity was evaluated through the Vibrio fischeri bioluminescence inhibition assay.Moreover,cytotoxicity was assessed at two time points(24-and 48-hr)through two complementary techniques in order to evaluate the cellular metabolic activity and membrane integrity of human lung epithelial cells A549.The Ames test using two Salmonella typhimurium strains(TA100 and TA98)was employed to assess the mutagenic potential of the polycyclic aromatic hydrocarbon fraction extracted from the PM10 samples.Results obtained with the bioluminescent bacteria indicated that only particles from the combustion of acacia pellets were toxic.All samples induced im-pairment on the A549 cells metabolic activity,while no significant release of lactate dehy-drogenase was recorded.PM10 emissions from acacia pellets were the most cytotoxic,while samples from both certified pellets evoked significant cytotoxicity at lower doses.Cytotox-icity time-dependency was only observed for PM10 from the combustion of acacia pellets and one of the brands of certified pellets.Mutagenic activity was not detected in both S.typhi murium strains.This study emphasises the role of the raw material for pellet manu-facturing on the toxicological profile of PM emissions.Alternative raw materials should be deeply investigated before their use in pelletisation and combustion in residential appli-ances.展开更多
文摘The new challenges that the phenomenon of demographic aging imposes on society in general, and health services in particular, can impact active and healthy aging. The project was implemented for two years （2012/2013） and aimed to combat loneliness in the elderly, which constitutes an obstacle to healthy and active aging. Decrease the sense of loneliness of individuals from 65 to 84 years of age, the project was made by using the health planning methodology, starting with a descriptive study （health diagnosis of the independent elderly population） followed by the development, implementation and evaluation of a community intervention project, which included home visits to the elderly, assessment of the level of loneliness in two different times （UCLA scale） and various intergenerational activities of social life （dancing, walking, online courses, games...） using a cross-section of partnerships and community involvement. There was a decrease from 62.8% to 41.9% in the reported overall feeling of loneliness of independent individuals between the ages of 65 and 84 years of age. There was an increase from 50.0% to 86.4% of seniors who now have dreams/life projects. The reduction of social and emotional isolation resulted in the promotion of social networks, which not only encouraged social interaction but also considered the elderly who live alone. The health gains arising from it are evidenced in contributing more proactively and the improvement of the quality of life in the elderly community.
基金the projects CICECOAveiro Institute of Materials(Ref.FCT UID/CTM/50011/2013)CESAM(Ref.FCT UID/AMB/50017/2013)+5 种基金financed by national funds through the FCT/MECco-financed by the European Regional Development Fund(FEDER)under the PT2020 Partnership AgreementFunding to the project FCOMP-01-0124-FEDER-021456(Ref.FCT PTDC/SAU-TOX/120953/2010)by FEDER through COMPETEnational funds through FCT,and the FCT-awarded grants(SFRH/BD/81792/2011SFRH/BPD/111736/2015SFRH/BPD/74868/2010)
文摘The antibacterial potential of silver nanoparticles（AgNPs） resulted in their increasing incorporation into consumer,industrial and biomedical products.Therefore,human and environmental exposure to AgNPs（either as an engineered product or a contaminant）supports the emergent research on the features conferring them different toxicity profiles.In this study,30 ran AgNPs coated with citrate or poly（ethylene glycol）（PEG） were used to assess the influence of coating on the effects produced on a human hepatoma cell line（HepG2）,namely in terms of viability,apoptosis,apoptotic related genes,cell cycle and cyclins gene expression.Both types of coated AgNPs decreased cell proliferation and viability with a similar toxicity profile.At the concentrations used（11 and 5 μg/mL corresponding to IC50 and-IC10 levels,respectively） the amount of cells undergoing apoptosis was not significant and the apoptotic related genes BCL2（anti-apoptotic gene）and BAX（pro-apoptotic gene） were both downregulated.Moreover,both AgNPs affected HepG2 cell cycle progression at the higher concentration（11 μg/mL） by increasing the percentage of cells in S（synthesis phase） and G2（Gap 2 phase） phases.Considering the cell-cycle related genes,the expression of cyclin B1 and cyclin E1 genes were decreased.Thus,this work has shown that citrate- and PEG-coated AgNPs impact on HepG2 apoptotic gene expression,cell cycle dynamics and cyclin regulation in a similar way.More research is needed to determine the properties that confer AgNPs at lower toxicity,since their use has proved helpful in several industrial and biomedical contexts.
基金We are also grateful for the support to CESAM(UIDB/50017/2020+UIDP/50017/2020)to FCT/MCTES through national funds,and co-funding by FEDER,within the PT2020 Partnership Agreement and Compete 2020+2 种基金This study was financially supported by the project“Chemical and toxicological SOurce PROfiling of particulate matter in urban air(SOPRO)”,POCI-01-0145-FEDER-029574,funded by FEDER,through COMPETE2020-Programa Operacional Competitividade e Internacionalizacao(POCI)by national funds(OE),through FCT/MCTES and Programa Operacional Regional de Lisboa 2020(Project N.007317).FCT is also acknowledged for the research contract under Scientific Employment Stimulus to H.Oliveira(CEECIND/04050/2017)The Vibrio fischeri bioluminescent inhibition tests were supported by the BIONANO_GINOP-2.3.2-15-2016-00017 project.
文摘Particulate matter emissions(PM10)from the combustion,in a residential stove,of two com-mercial brands of certified(ENplus A1)pellets,a non-certified brand and laboratory made pellets of acacia were tested for their ability to induce ecotoxic,cytotoxic,and mutagenic re-sponses in unicellular organisms and a human cell line.Ecotoxicity was evaluated through the Vibrio fischeri bioluminescence inhibition assay.Moreover,cytotoxicity was assessed at two time points(24-and 48-hr)through two complementary techniques in order to evaluate the cellular metabolic activity and membrane integrity of human lung epithelial cells A549.The Ames test using two Salmonella typhimurium strains(TA100 and TA98)was employed to assess the mutagenic potential of the polycyclic aromatic hydrocarbon fraction extracted from the PM10 samples.Results obtained with the bioluminescent bacteria indicated that only particles from the combustion of acacia pellets were toxic.All samples induced im-pairment on the A549 cells metabolic activity,while no significant release of lactate dehy-drogenase was recorded.PM10 emissions from acacia pellets were the most cytotoxic,while samples from both certified pellets evoked significant cytotoxicity at lower doses.Cytotox-icity time-dependency was only observed for PM10 from the combustion of acacia pellets and one of the brands of certified pellets.Mutagenic activity was not detected in both S.typhi murium strains.This study emphasises the role of the raw material for pellet manu-facturing on the toxicological profile of PM emissions.Alternative raw materials should be deeply investigated before their use in pelletisation and combustion in residential appli-ances.
