Battery safety is influenced by various factors,with thermal runaway being one of the most significant concerns.While most studies have concentrated on developing one-time,self-activating mechanism for thermal protect...Battery safety is influenced by various factors,with thermal runaway being one of the most significant concerns.While most studies have concentrated on developing one-time,self-activating mechanism for thermal protection,such as temperature-responsive electrodes,and thermal-shutdown separators,these methods only provide irreversible protection.Recently,reversible temperature-sensitive electrolytes have emerged as promising alternatives,offering both thermo-reversibility and self-protective properties.However,further research is crucial to fully understand these thermal-shutdown electrolytes.In this study,we propose lower critical solution temperature(LCST)phase behavior poly(benzyl methacrylate)/imidazolium-based ionic liquid mixtures to prepare temperature-sensitive electrolytes that provide reversible thermal shutdown protection of batteries.This electrolyte features an appropriate protection temperature(~105℃)and responds quickly within a 1 min at 105℃,causing cells to hardly discharge as the voltage suddenly drops to 3.38 V,and providing efficient thermal shutdown protection within 30 min.Upon cooling back to room temperature,the battery regains its original performance.Additionally,the electrolyte exhibits excellent cycling stability with the capacity retention of the battery is 91.6%after 500 cycles.This work provides a viable solution for preventing batteries from thermal runaway triggered by overheating.展开更多
Exosomes secreted by tumor cells carry abundant molecular biomarkers that reflect the status of their originating cells.These tumor-derived exosomes(TDEs)have emerged as attractive diagnostic targets.However,the ident...Exosomes secreted by tumor cells carry abundant molecular biomarkers that reflect the status of their originating cells.These tumor-derived exosomes(TDEs)have emerged as attractive diagnostic targets.However,the identification and characterization of highly heterogeneous TDEs remain practically challenging.Here,we report a dual rolling circle amplification(DRCA)-assisted approach for the selective encapsulation of single TDEs for fluorescence microscopic and flow cytometric analysis.TDEs have been targeted by aptamers that recognized their surface tumor marker and exosomal marker CD63,following DRCA that produced entangling polymeric DNA chains,resulting in facile particle enlargement that allows single-particle fluorescence profiling of exosome heterogeneity.We have demonstrated the use of a dual-marker positive ratio for exosome differentiation and applied division and multiplication operations for normalized andmagnified marker heterogeneity analysis.We further applied this assay to distinguish lung adenocarcinoma and pulmonary nodule patients and found an accuracy of 90%.We anticipate promising transformations of this straightforward assay into clinically implantable diagnostic methods.展开更多
基金funded by the National Natural Science Foundation of China(no.22075155)the Zhejiang Provincial Natural Science Foundation of China(No.LY24B030002)+2 种基金Ningbo Natural Science Foundation(2023J089)the China Scholarship Council(CSC)the Ningbo Science and Technology Bureau(2024QL036).
文摘Battery safety is influenced by various factors,with thermal runaway being one of the most significant concerns.While most studies have concentrated on developing one-time,self-activating mechanism for thermal protection,such as temperature-responsive electrodes,and thermal-shutdown separators,these methods only provide irreversible protection.Recently,reversible temperature-sensitive electrolytes have emerged as promising alternatives,offering both thermo-reversibility and self-protective properties.However,further research is crucial to fully understand these thermal-shutdown electrolytes.In this study,we propose lower critical solution temperature(LCST)phase behavior poly(benzyl methacrylate)/imidazolium-based ionic liquid mixtures to prepare temperature-sensitive electrolytes that provide reversible thermal shutdown protection of batteries.This electrolyte features an appropriate protection temperature(~105℃)and responds quickly within a 1 min at 105℃,causing cells to hardly discharge as the voltage suddenly drops to 3.38 V,and providing efficient thermal shutdown protection within 30 min.Upon cooling back to room temperature,the battery regains its original performance.Additionally,the electrolyte exhibits excellent cycling stability with the capacity retention of the battery is 91.6%after 500 cycles.This work provides a viable solution for preventing batteries from thermal runaway triggered by overheating.
基金supported by the National Key Research Program(grant no.2019YFA0905800)the NSFC Program(grant no.22090053)the Natural Science Foundation of Hunan Province(grant no.2021JJ40040).
文摘Exosomes secreted by tumor cells carry abundant molecular biomarkers that reflect the status of their originating cells.These tumor-derived exosomes(TDEs)have emerged as attractive diagnostic targets.However,the identification and characterization of highly heterogeneous TDEs remain practically challenging.Here,we report a dual rolling circle amplification(DRCA)-assisted approach for the selective encapsulation of single TDEs for fluorescence microscopic and flow cytometric analysis.TDEs have been targeted by aptamers that recognized their surface tumor marker and exosomal marker CD63,following DRCA that produced entangling polymeric DNA chains,resulting in facile particle enlargement that allows single-particle fluorescence profiling of exosome heterogeneity.We have demonstrated the use of a dual-marker positive ratio for exosome differentiation and applied division and multiplication operations for normalized andmagnified marker heterogeneity analysis.We further applied this assay to distinguish lung adenocarcinoma and pulmonary nodule patients and found an accuracy of 90%.We anticipate promising transformations of this straightforward assay into clinically implantable diagnostic methods.
