Background:To explore the effect of electroacupuncture Wushu acupoints on angiogenesis and expressions of brain-derived neurotrophic factor(BDNF),as well as to explore the possible mechanism of electroacupuncture on a...Background:To explore the effect of electroacupuncture Wushu acupoints on angiogenesis and expressions of brain-derived neurotrophic factor(BDNF),as well as to explore the possible mechanism of electroacupuncture on acute myocardial ischemia(AMI).Methods:We randomly divided 42 Sprague Dawley rats into sham AMI group,AMI group,and the heart channel groups comprising Shaochong(HT9)acupoint,Shaofu(HT8)acupoint,Shenmen(HT7)acupoint,Lingdao(HT4)acupoint,and Shaohai(HT3)acupoint groups,with 6 rats in each group.The AMI model was fabricated via hypodesmus of the left anterior descending coronary artery.After modeling,the corresponding acupoints of each group underwent electroacupuncture treatment 30 min/time,once/day for 3 consecutive days.The cardiogram obtained before and after the intervention was compared,and the pathologic changes of the myocardial tissue were observed via hematoxylin&eosin staining.The amount of serum endothelin-1,prostacycline-2,thromboxane-2,and BDNF indicators were assayed using ELISA,the number of CD31-positive cells in myocardial tissue was calculated using immunohistochemistry,and the expression levels of BDNF and TrKB was measured using western blot.Result:Compared with the sham AMI group,the ST segment on the electrocardiogram was significantly elevated(P<0.05);the myocardial angiogenesis marker CD31 was remarkably increased(P<0.05);serum levels of endothelin-1,prostacycline-2 and prostacycline-2/thromboxane-2 were decreased(P<0.05),whereas serum levels of thromboxane-2 levels were increased(P<0.05);and those of serum BDNF was decreased(P<0.05);the protein overexpression contents of BDNF and TrKB in the myocardial tissues of HT8 acpoint and Shenmen acpoint groups were reduced(P<0.05)in the AMI group.Compared with the AMI group,in the heart channel groups,the ST segment of electrocardiogram was dramatically reduced(P<0.05);CD31 was dramatically elevated(P<0.05);the serum levels of endothelin-1,prostacycline-2,and prostacycline-2/thromboxane-2 were increased(P<0.05),thromboxane-2 levels were reduced(P<0.05);the serum BDNF level was increased(P<0.05);the protein overexpression levels of BDNF and TrKB in myocardial tissue of HT8 group and Shenmen group was increased(P<0.05).Conclusion:electroacupuncture of Wushu acupoints along the heart channel may enhance myocardial ischemia by promoting overexpression of serum BDNF and activating the BDNF/TrKB pathway to promote myocardial angiogenesis.展开更多
Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure tre...Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure treatment. Methods: Healthy male Sprague-Dawley rats were allocated into three groups: Sham group, Model group, and electroacupuncture (Model + EA) group, with each group comprising 8 rats. The model underwent a procedure involving the ligation of the left anterior descending coronary artery to induce a model of heart failure. The Model + EA group was used for 7 consecutive days for electroacupuncture of bilateral Shenmen (HT7) and Tongli (HT5), once a day for 30 min each time. Left ventricular parameters in rats were assessed using a small-animal ultrasound machine to analyze changes in left ventricular end-diastolic volume, left ventricular end-systolic volume, left ventricular ejection fraction, and left ventricular fractional shortening. Serum interleukin-1β (IL-1β), cardiac troponin (cTn), and N-terminal brain natriuretic peptide precursor levels were measured using ELISA. Histopathological changes in rat myocardium were observed through HE staining, while collagen deposition in rat myocardial tissue was assessed using the Masson staining method. Picro sirius red staining, immunohistochemical staining, and RT-qPCR were utilized to distinguish between the various types of collagen deposition. The expression level of TGF-β1 and SMAD2/3/4/7 mRNA in rat myocardial tissues was determined using RT-qPCR. Additionally, western blot analysis was conducted to assess the protein expression levels of TGF-β1, SMAD3/7, and p-SMAD3 in rat myocardial tissues. Results: Compared with the Sham group, the left ventricular ejection fraction and left ventricular fractional shortening values of the Model group were significantly decreased (P < 0.01);the left ventricular end-diastolic volume and left ventricular end-systolic volume values were remarkably increased (P < 0.01);serum N-terminal brain natriuretic peptide precursor content was increased (P < 0.01);serum IL-1β and cTn levels were increased (P < 0.01);myocardial collagen volume fraction were increased (P < 0.01);and those of the expression of TGF-β1 and SMAD2/3/4 mRNA was increased (P < 0.01);the expression of SMAD7 mRNA was decreased (P < 0.01);the protein expression levels of TGF-β1, SMAD3, and p-Smad3 were increased (P < 0.01);the protein expression level of SMAD7 was decreased (P < 0.01) in the Model group. Compared to the Model group, the expression levels of the proteins TGF-β1, SMAD3, and p-Smad3 in myocardial tissue were found to be decreased (P < 0.01), and the expression level of the protein SMAD7 was found to be increased (P < 0.01) in the Model + EA group;the collagen volume fraction and deposition of type Ⅰ /Ⅲ collagen were decreased (P < 0.01) in the Model + EA group. Conclusion: Electroacupuncture alleviates myocardial fibrosis in rats with heart failure, and this effect is likely due to attributed to the modulation of the TGF-β1/Smads signaling pathway, which helps reduce collagen deposition in the extracellular matrix.展开更多
基金supported by the the National Natural Science Foundation of China(No.82274303)the China's National key Research and development program projects(No.2022YFC3500500 and No.2022YFC3500502).
文摘Background:To explore the effect of electroacupuncture Wushu acupoints on angiogenesis and expressions of brain-derived neurotrophic factor(BDNF),as well as to explore the possible mechanism of electroacupuncture on acute myocardial ischemia(AMI).Methods:We randomly divided 42 Sprague Dawley rats into sham AMI group,AMI group,and the heart channel groups comprising Shaochong(HT9)acupoint,Shaofu(HT8)acupoint,Shenmen(HT7)acupoint,Lingdao(HT4)acupoint,and Shaohai(HT3)acupoint groups,with 6 rats in each group.The AMI model was fabricated via hypodesmus of the left anterior descending coronary artery.After modeling,the corresponding acupoints of each group underwent electroacupuncture treatment 30 min/time,once/day for 3 consecutive days.The cardiogram obtained before and after the intervention was compared,and the pathologic changes of the myocardial tissue were observed via hematoxylin&eosin staining.The amount of serum endothelin-1,prostacycline-2,thromboxane-2,and BDNF indicators were assayed using ELISA,the number of CD31-positive cells in myocardial tissue was calculated using immunohistochemistry,and the expression levels of BDNF and TrKB was measured using western blot.Result:Compared with the sham AMI group,the ST segment on the electrocardiogram was significantly elevated(P<0.05);the myocardial angiogenesis marker CD31 was remarkably increased(P<0.05);serum levels of endothelin-1,prostacycline-2 and prostacycline-2/thromboxane-2 were decreased(P<0.05),whereas serum levels of thromboxane-2 levels were increased(P<0.05);and those of serum BDNF was decreased(P<0.05);the protein overexpression contents of BDNF and TrKB in the myocardial tissues of HT8 acpoint and Shenmen acpoint groups were reduced(P<0.05)in the AMI group.Compared with the AMI group,in the heart channel groups,the ST segment of electrocardiogram was dramatically reduced(P<0.05);CD31 was dramatically elevated(P<0.05);the serum levels of endothelin-1,prostacycline-2,and prostacycline-2/thromboxane-2 were increased(P<0.05),thromboxane-2 levels were reduced(P<0.05);the serum BDNF level was increased(P<0.05);the protein overexpression levels of BDNF and TrKB in myocardial tissue of HT8 group and Shenmen group was increased(P<0.05).Conclusion:electroacupuncture of Wushu acupoints along the heart channel may enhance myocardial ischemia by promoting overexpression of serum BDNF and activating the BDNF/TrKB pathway to promote myocardial angiogenesis.
基金the China’s National Key Research and Development Program Projects(No.2022YFC3500500 and No.2022YFC3500502).
文摘Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure treatment. Methods: Healthy male Sprague-Dawley rats were allocated into three groups: Sham group, Model group, and electroacupuncture (Model + EA) group, with each group comprising 8 rats. The model underwent a procedure involving the ligation of the left anterior descending coronary artery to induce a model of heart failure. The Model + EA group was used for 7 consecutive days for electroacupuncture of bilateral Shenmen (HT7) and Tongli (HT5), once a day for 30 min each time. Left ventricular parameters in rats were assessed using a small-animal ultrasound machine to analyze changes in left ventricular end-diastolic volume, left ventricular end-systolic volume, left ventricular ejection fraction, and left ventricular fractional shortening. Serum interleukin-1β (IL-1β), cardiac troponin (cTn), and N-terminal brain natriuretic peptide precursor levels were measured using ELISA. Histopathological changes in rat myocardium were observed through HE staining, while collagen deposition in rat myocardial tissue was assessed using the Masson staining method. Picro sirius red staining, immunohistochemical staining, and RT-qPCR were utilized to distinguish between the various types of collagen deposition. The expression level of TGF-β1 and SMAD2/3/4/7 mRNA in rat myocardial tissues was determined using RT-qPCR. Additionally, western blot analysis was conducted to assess the protein expression levels of TGF-β1, SMAD3/7, and p-SMAD3 in rat myocardial tissues. Results: Compared with the Sham group, the left ventricular ejection fraction and left ventricular fractional shortening values of the Model group were significantly decreased (P < 0.01);the left ventricular end-diastolic volume and left ventricular end-systolic volume values were remarkably increased (P < 0.01);serum N-terminal brain natriuretic peptide precursor content was increased (P < 0.01);serum IL-1β and cTn levels were increased (P < 0.01);myocardial collagen volume fraction were increased (P < 0.01);and those of the expression of TGF-β1 and SMAD2/3/4 mRNA was increased (P < 0.01);the expression of SMAD7 mRNA was decreased (P < 0.01);the protein expression levels of TGF-β1, SMAD3, and p-Smad3 were increased (P < 0.01);the protein expression level of SMAD7 was decreased (P < 0.01) in the Model group. Compared to the Model group, the expression levels of the proteins TGF-β1, SMAD3, and p-Smad3 in myocardial tissue were found to be decreased (P < 0.01), and the expression level of the protein SMAD7 was found to be increased (P < 0.01) in the Model + EA group;the collagen volume fraction and deposition of type Ⅰ /Ⅲ collagen were decreased (P < 0.01) in the Model + EA group. Conclusion: Electroacupuncture alleviates myocardial fibrosis in rats with heart failure, and this effect is likely due to attributed to the modulation of the TGF-β1/Smads signaling pathway, which helps reduce collagen deposition in the extracellular matrix.
