From four Korean pine （Pinus koraiensis） orchards, 60 clones were selected and analyzed for the fatty acid and amino acid components of the seeds to reveal the variations and correlations of the seed characteristics...From four Korean pine （Pinus koraiensis） orchards, 60 clones were selected and analyzed for the fatty acid and amino acid components of the seeds to reveal the variations and correlations of the seed characteristics among the clonal source orchards and clones. The nutri- tional components of the seeds of the P. koraiensis trees exhibited rich genetic variation; the variation coefficient of the fatty acids was 2.24-66.83 %, while the variation coefficient of the amino acids was 14.70-38.88 %. Rela- tively high genetic-improvement potential exists for the nutritional components of the seeds. The phenotypic dif- ferentiation of the fatty acid and amino acid components reveals that variation within the population （85.18 %） was the primary source for the variation of the fatty acid components; variation among the orchards （63.08 %） was the primary source of the variation of the amino acid components. Data drawn from various clonal source orchards all showed that the seed characteristics were highly controlled by heritability （h2 〉 80 %）, and the seed characteristics of the P. koraiensis trees exhibited a similar genetic gain trend. The principal components were ana- lyzed to obtain the comprehensive principal component value for each clonal seed orchard. Twelve clones were selected based on a clonal selection rate of 20 %. Corre- lation and multiple stepwise-regression analyses were conducted, considering different location conditions, to reveal the stable correlations between the seed character- istics to facilitate improvements of the seed yield of P. koraiensis trees and the clonal selection. Species of real characteristics in P. koraiensis were controlled by higher heritability. Genetic gain was obtained by selecting of superior clones.展开更多
With embryogenic callus of Larix olgensisis, we investigated the effects of inositol, glutamine, casein hydrolysate, carbohydrate, abscisic acid and silver nitrate concentration on the maturation of the somatic embryo...With embryogenic callus of Larix olgensisis, we investigated the effects of inositol, glutamine, casein hydrolysate, carbohydrate, abscisic acid and silver nitrate concentration on the maturation of the somatic embryo.Three dominant factors emerged, and we developed a response surface model based on the Box-Behnken design.We defined the optimal conditions for the maturation of somatic embryos. The contents of abscisic acid, silver nitrate, sucrose and casein hydrolysis significantly affected the amount of maturing embryos, but inositol, maltose and glutamine had no effect. By establishing a response surface model with multiple factors, we predicted that the optimal number of L. olgensis somatic embryos was 204 ± 4 gon basal medium, containing 18.28 mg Labscisic acid,5.46 mg Lsilver nitrate and 82.67 g Lsucrose. In the verification experiments, the addition of 20 mg Labscisic acid, 5 mg Lsilver nitrate and 80 g Lsucrose to BM yielded an average of 202.06 somatic embryos per gram. These results should guide large-scale breeding of L. olgensis.展开更多
Picea spp.are a prominent component of the boreal,montane,and sub-alpine forests in the Northern Hemisphere,and have substantial economic importance due to their high quality fibre.However,performance and wood propert...Picea spp.are a prominent component of the boreal,montane,and sub-alpine forests in the Northern Hemisphere,and have substantial economic importance due to their high quality fibre.However,performance and wood properties of the genus have not been systematically evaluated in China.This study aimed to examine genetic variations in growth traits,phenology,and wood properties of 17 Picea species,(three from North America,two from Europe,and 12 from China),in response to specific climate factors using a randomized complete block design in a monsoonal,middle latitude area of China.Results show that all growth traits and wood properties significantly varied among species(P <0.00).Of the 17 species examined,P.abies and P.pungens were the tallest with heights of 2.5 and 1.9 m at 9 years old,respectively,90% and 50% greater than the average heights.Branch length,number,and angles of both P.abies and P.pungens were greater than those of the other species.Heights of P.glauca and P.omorika were20-33% greater than the average.Fast-growing species had high quantities of first lateral branches and large top whorl branch lengths.The taller species exhibited greater tracheid lengths and average tracheid lengths to radial central diameter ratios(TL/R_D2),but smaller cell wall thicknesses to tracheid radial lumen diameter ratios(WT/R_D1),which is favorable for pulp production.Correlation analysis revealed that height and ring width had significant positive correlations with latitude but strongly negative correlations with longitude.Height was positively correlated with average annual rainfalls,but negative correlations with average yearly sunshine.Due to the increased average annual sunshine at the China test site relative to the seed source,species such as P.abies,P.pungens,P.glauca,and P.omorika from wet,humid areas performed better than native species.Considering similar temperatures,precipitation may be the main factor affecting growth,which is beneficial for predicting the extent of spruce expansion.These results should encourage further testing and provide reference information for future exotic species studies in this part of China.展开更多
Fraxinus mandshurica Rupr.is one of the main afforestation species in northeast China,and there is great demand for improved F.mandshurica varieties.The results of an investigation into and analysis of the growth trai...Fraxinus mandshurica Rupr.is one of the main afforestation species in northeast China,and there is great demand for improved F.mandshurica varieties.The results of an investigation into and analysis of the growth traits of F.mandshurica provenances and families showed that there were significant differences in different periods.However,variations in growth traits decreased year by year.There was a significant negative correlation between tree heights of the provenances and sunshine hours in their areas of origin.The provenances of Xinglong,Hailin and Wuchang were selected based on the volume of 18-year-old trees.The average genetic gain from the selection of fast-growing provenances was 19.4%.Ten superior fast-growing families were selected.The average volume of the selected families was22.6%,higher than that of all families.The correlation coefficient between heights at 6-year-old and at 18a was 0.838for provenances,and between heights at 4-year-old and at 18-year-old was 0.303 for families.These results indicate that early selection for height in provenances or families could be performed at 6 years or 4 years,respectively.Early selection for DBH and volume in families could start at 8 years.展开更多
The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC...The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC genes in Larix olgensis can be used to analyse the function of the NAC gene in the future.Screening of excellent genetic materials and molecular breeding have been utilized to cultivate high-quality,stress-resistant larches.According to the transcriptome data for L.olgensis,the genes Uni-gene81490 and Unigene70699 with complete ORFs(open reading frames)were obtained by conserved domain analy-sis and named LoNAC1 and LoNAC2,respectively.The cDNAs of LoNAC1 and LoNAC2 were 1971 bp and 1095 bp in length,encoding 656 and 364 amino acids,respectively.The molecular weights of the proteins encoded by the two genes were predicted to be 72.61 kDa and 41.13 kDa,and subcellular localization analysis indicated that the proteins were concentrated in the nucleus.The results of real-time quantitative PCR analysis showed that at different growth stages and in different tissues of L.olgensis,the relative expression levels of the two NAC genes were highest in the stem,and the expression differences were more obvious in non-lignified tissues.After drought,salt and alkali stress and hormone treatment,expression was induced to different degrees.The expression levels of LoNAC1 and LoNAC2 in semi-lignified L.olgensis were higher than in the other two periods(non-lignified and lignified),and expression levels significantly increased under drought and salt stress.Relative expression levels changed under hormone treatment.It is speculated that these two genes may not only be related to drought and salt stress and secondary growth but may also be induced by hormones such as abscisic acid.Overall,LoNAC1 and LoNAC2 are genetic materials that can be used for molecular breeding of larch.展开更多
Larix olgensis A.Henry is a fast-growing tree used for aff orestation in northeastern China and has great ecological and economic value.For studying developmental genes in the xylem of this species,we investigated the...Larix olgensis A.Henry is a fast-growing tree used for aff orestation in northeastern China and has great ecological and economic value.For studying developmental genes in the xylem of this species,we investigated the Myb transcription factor family,one of the largest families of transcription factors in plants,which plays an important role in the regulation of lignifi cation in plant secondary walls.By sequencing a L.olgensis cDNA library using the Illumina HiSeq2500 high-throughput sequencing platform,we obtained 58,683 unigene sequences,of which 16,554 unigenes were longer than 1000 bp,accounting for 28.2%of the total database.The alignment of these genes with the GO,COG,KEGG,Swiss-Prot and NR databases resulted in annotated 29,350 unigenes.We obtained a total of 1460 differentially expressed genes,of which 453 were upregulated and 1007 were downregulated at the two developmental stages analyzed.The gene annotations showed a wide range of biological functions and metabolic pathways.The 10 Myb transcription factors that were obtained from the diff erentially expressed genes were analyzed by real-time quantitative PCR(qRT-PCR).The results showed that four Myb transcription factors may be associated with xylem development in L.olgensis.Due to the large genome size of conifers,genomics research on these species has lagged behind that for other plant groups.Our data provide the basis for further studies on xylem development in L.olgensis.展开更多
基金supported by the Special Research of Public Welfare Forestry Industry for Financial Support for ‘‘Korean pine Improved Type Selection and Directive Breeding Technology Research(201204320)’’the Fundamental Research Funds for the Central Universities(2572014AA23)
文摘From four Korean pine （Pinus koraiensis） orchards, 60 clones were selected and analyzed for the fatty acid and amino acid components of the seeds to reveal the variations and correlations of the seed characteristics among the clonal source orchards and clones. The nutri- tional components of the seeds of the P. koraiensis trees exhibited rich genetic variation; the variation coefficient of the fatty acids was 2.24-66.83 %, while the variation coefficient of the amino acids was 14.70-38.88 %. Rela- tively high genetic-improvement potential exists for the nutritional components of the seeds. The phenotypic dif- ferentiation of the fatty acid and amino acid components reveals that variation within the population （85.18 %） was the primary source for the variation of the fatty acid components; variation among the orchards （63.08 %） was the primary source of the variation of the amino acid components. Data drawn from various clonal source orchards all showed that the seed characteristics were highly controlled by heritability （h2 〉 80 %）, and the seed characteristics of the P. koraiensis trees exhibited a similar genetic gain trend. The principal components were ana- lyzed to obtain the comprehensive principal component value for each clonal seed orchard. Twelve clones were selected based on a clonal selection rate of 20 %. Corre- lation and multiple stepwise-regression analyses were conducted, considering different location conditions, to reveal the stable correlations between the seed character- istics to facilitate improvements of the seed yield of P. koraiensis trees and the clonal selection. Species of real characteristics in P. koraiensis were controlled by higher heritability. Genetic gain was obtained by selecting of superior clones.
基金supported by the National High-tech R&D Program(863 Program)of China(2013AA102704)
文摘With embryogenic callus of Larix olgensisis, we investigated the effects of inositol, glutamine, casein hydrolysate, carbohydrate, abscisic acid and silver nitrate concentration on the maturation of the somatic embryo.Three dominant factors emerged, and we developed a response surface model based on the Box-Behnken design.We defined the optimal conditions for the maturation of somatic embryos. The contents of abscisic acid, silver nitrate, sucrose and casein hydrolysis significantly affected the amount of maturing embryos, but inositol, maltose and glutamine had no effect. By establishing a response surface model with multiple factors, we predicted that the optimal number of L. olgensis somatic embryos was 204 ± 4 gon basal medium, containing 18.28 mg Labscisic acid,5.46 mg Lsilver nitrate and 82.67 g Lsucrose. In the verification experiments, the addition of 20 mg Labscisic acid, 5 mg Lsilver nitrate and 80 g Lsucrose to BM yielded an average of 202.06 somatic embryos per gram. These results should guide large-scale breeding of L. olgensis.
基金supported by the Thirteenth Five-Year Plan for Key & Research Projects of China(2017YFD0600606-09)the National Natural Science Foundation of China (NSFC31600541)the Science Foundation of China Post-doctor (2016M591053)。
文摘Picea spp.are a prominent component of the boreal,montane,and sub-alpine forests in the Northern Hemisphere,and have substantial economic importance due to their high quality fibre.However,performance and wood properties of the genus have not been systematically evaluated in China.This study aimed to examine genetic variations in growth traits,phenology,and wood properties of 17 Picea species,(three from North America,two from Europe,and 12 from China),in response to specific climate factors using a randomized complete block design in a monsoonal,middle latitude area of China.Results show that all growth traits and wood properties significantly varied among species(P <0.00).Of the 17 species examined,P.abies and P.pungens were the tallest with heights of 2.5 and 1.9 m at 9 years old,respectively,90% and 50% greater than the average heights.Branch length,number,and angles of both P.abies and P.pungens were greater than those of the other species.Heights of P.glauca and P.omorika were20-33% greater than the average.Fast-growing species had high quantities of first lateral branches and large top whorl branch lengths.The taller species exhibited greater tracheid lengths and average tracheid lengths to radial central diameter ratios(TL/R_D2),but smaller cell wall thicknesses to tracheid radial lumen diameter ratios(WT/R_D1),which is favorable for pulp production.Correlation analysis revealed that height and ring width had significant positive correlations with latitude but strongly negative correlations with longitude.Height was positively correlated with average annual rainfalls,but negative correlations with average yearly sunshine.Due to the increased average annual sunshine at the China test site relative to the seed source,species such as P.abies,P.pungens,P.glauca,and P.omorika from wet,humid areas performed better than native species.Considering similar temperatures,precipitation may be the main factor affecting growth,which is beneficial for predicting the extent of spruce expansion.These results should encourage further testing and provide reference information for future exotic species studies in this part of China.
基金Promotion project of State Forestry Administration(2017)06,China。
文摘Fraxinus mandshurica Rupr.is one of the main afforestation species in northeast China,and there is great demand for improved F.mandshurica varieties.The results of an investigation into and analysis of the growth traits of F.mandshurica provenances and families showed that there were significant differences in different periods.However,variations in growth traits decreased year by year.There was a significant negative correlation between tree heights of the provenances and sunshine hours in their areas of origin.The provenances of Xinglong,Hailin and Wuchang were selected based on the volume of 18-year-old trees.The average genetic gain from the selection of fast-growing provenances was 19.4%.Ten superior fast-growing families were selected.The average volume of the selected families was22.6%,higher than that of all families.The correlation coefficient between heights at 6-year-old and at 18a was 0.838for provenances,and between heights at 4-year-old and at 18-year-old was 0.303 for families.These results indicate that early selection for height in provenances or families could be performed at 6 years or 4 years,respectively.Early selection for DBH and volume in families could start at 8 years.
基金This project was supported by the National Science and Technology Major Project(2018ZX08020003-001-001)the National Natural Science Foundation of China(Grant No.31700595)+1 种基金the Fundamental Research Funds for the Central Universities(2572019BA13)Heilongjiang Touyan Innovation Team Program.
文摘The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC genes in Larix olgensis can be used to analyse the function of the NAC gene in the future.Screening of excellent genetic materials and molecular breeding have been utilized to cultivate high-quality,stress-resistant larches.According to the transcriptome data for L.olgensis,the genes Uni-gene81490 and Unigene70699 with complete ORFs(open reading frames)were obtained by conserved domain analy-sis and named LoNAC1 and LoNAC2,respectively.The cDNAs of LoNAC1 and LoNAC2 were 1971 bp and 1095 bp in length,encoding 656 and 364 amino acids,respectively.The molecular weights of the proteins encoded by the two genes were predicted to be 72.61 kDa and 41.13 kDa,and subcellular localization analysis indicated that the proteins were concentrated in the nucleus.The results of real-time quantitative PCR analysis showed that at different growth stages and in different tissues of L.olgensis,the relative expression levels of the two NAC genes were highest in the stem,and the expression differences were more obvious in non-lignified tissues.After drought,salt and alkali stress and hormone treatment,expression was induced to different degrees.The expression levels of LoNAC1 and LoNAC2 in semi-lignified L.olgensis were higher than in the other two periods(non-lignified and lignified),and expression levels significantly increased under drought and salt stress.Relative expression levels changed under hormone treatment.It is speculated that these two genes may not only be related to drought and salt stress and secondary growth but may also be induced by hormones such as abscisic acid.Overall,LoNAC1 and LoNAC2 are genetic materials that can be used for molecular breeding of larch.
文摘Larix olgensis A.Henry is a fast-growing tree used for aff orestation in northeastern China and has great ecological and economic value.For studying developmental genes in the xylem of this species,we investigated the Myb transcription factor family,one of the largest families of transcription factors in plants,which plays an important role in the regulation of lignifi cation in plant secondary walls.By sequencing a L.olgensis cDNA library using the Illumina HiSeq2500 high-throughput sequencing platform,we obtained 58,683 unigene sequences,of which 16,554 unigenes were longer than 1000 bp,accounting for 28.2%of the total database.The alignment of these genes with the GO,COG,KEGG,Swiss-Prot and NR databases resulted in annotated 29,350 unigenes.We obtained a total of 1460 differentially expressed genes,of which 453 were upregulated and 1007 were downregulated at the two developmental stages analyzed.The gene annotations showed a wide range of biological functions and metabolic pathways.The 10 Myb transcription factors that were obtained from the diff erentially expressed genes were analyzed by real-time quantitative PCR(qRT-PCR).The results showed that four Myb transcription factors may be associated with xylem development in L.olgensis.Due to the large genome size of conifers,genomics research on these species has lagged behind that for other plant groups.Our data provide the basis for further studies on xylem development in L.olgensis.
