Lipid metabolism disorders commonly occur during menopause.Estrogen deficiency has been shown to lead to excessive energy intake and abnormal lipid metabolism in ovariectomized rats,resulting in obesity.Probiotics exh...Lipid metabolism disorders commonly occur during menopause.Estrogen deficiency has been shown to lead to excessive energy intake and abnormal lipid metabolism in ovariectomized rats,resulting in obesity.Probiotics exhibit anti-obesity properties,and their underlying mechanism has been widely reported.In this study,we demonstrated the metabolic benefits of Lactobacillus plantarum CCFM1180 in suppressing appetite,controlling body weight,correcting obesity-induced abnormalities,enhancing liver lipid metabolism,and protecting liver function in estrogen-deficient rats.The mechanisms associated with the anti-obesity and anti-dyslipidemia effects of CCFM1180 on estrogen-deficient rats were clarified.The results showed that CCFM1180 dramatically reduced food intake by activating the expression of estrogen receptor alpha(ERα)and increasing the level of leptin in abdominal adipose tissue.These changes,combined with the increased butyrate concentration and recovered bile acid structure,helped enhance lipid metabolism.Additionally,CCFM1180 treatment was found to be safer than exogenous estrogen supplementation.Thus,L.plantarum CCFM1180 could be considered a new therapeutic strategy for preventing and alleviating menopausal lipid abnormalities.展开更多
The decline in ovarian estrogen production is known to have detrimental health consequences and negatively affect the quality of life in menopausal women.Increasing estradiol levels is key to preventing disease in men...The decline in ovarian estrogen production is known to have detrimental health consequences and negatively affect the quality of life in menopausal women.Increasing estradiol levels is key to preventing disease in menopausal women.In this study,Lactobacillus gasseri CCFM1255 isolated from healthy infants was found to have a positive effect on estradiol production in ovariectomized rats.CYP19,the key enzyme catalysing the conversion of androgens into estrogens,was upregulated in adipose tissue upon CCFM1255 treatment.Untargeted metabolome analysis and targeted metabolite detection were used to identify the key metabolites altered by CCFM1255 treatment.CCFM1255 treatment significantly improved the serum concentration of glutamine(Gln).A significantly positive correlation was observed between serum Gln and estradiol concentrations.CCFM1255 treatment reshaped the structure of the gut microbiome,which was correlated with certain changes in serum metabolite concentrations.These results indicate that the provision of CCFM1255 as a dietary supplement may be an effective strategy to alleviate menopausal symptoms by increasing circulating estradiol.展开更多
Use of data assimilation to initialize hydrometeors plays a vital role in numerical weather prediction(NWP).To directly analyze hydrometeors in data assimilation systems from cloud-sensitive observations,hydrometeor c...Use of data assimilation to initialize hydrometeors plays a vital role in numerical weather prediction(NWP).To directly analyze hydrometeors in data assimilation systems from cloud-sensitive observations,hydrometeor control variables are necessary.Common data assimilation systems theoretically require that the probability density functions(PDFs)of analysis,background,and observation errors should satisfy the Gaussian unbiased assumptions.In this study,a Gaussian transform method is proposed to transform hydrometeors to more Gaussian variables,which is modified from the Softmax function and renamed as Quasi-Softmax transform.The Quasi-Softmax transform method then is compared to the original hydrometeor mixing ratios and their logarithmic transform and Softmax transform.The spatial distribution,the non-Gaussian nature of the background errors,and the characteristics of the background errors of hydrometeors in each method are studied.Compared to the logarithmic and Softmax transform,the Quasi-Softmax method keeps the vertical distribution of the original hydrometeor mixing ratios to the greatest extent.The results of the D′Agostino test show that the hydrometeors transformed by the Quasi-Softmax method are more Gaussian when compared to the other methods.The Gaussian transform has been added to the control variable transform to estimate the background error covariances.Results show that the characteristics of the hydrometeor background errors are reasonable for the Quasi-Softmax method.The transformed hydrometeors using the Quasi-Softmax transform meet the Gaussian unbiased assumptions of the data assimilation system,and are promising control variables for data assimilation systems.展开更多
This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice.Newborn BALB/c mice were assigned to the control group(administration s...This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice.Newborn BALB/c mice were assigned to the control group(administration saline)and the CCFM1078 group(administration B.breve CCFM1078,3×10^(9) CFU/day)in 3-,4-,and 5-week tests.All the groups have male and female distinctions.Our findings demonstrate that B.breve CCFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites(hexadecanamide,linoleoyl ethanolamide,and palmitoyl ethanolamide),the genes and proteins expression related to the growth hormone(GH)/insulin-like growth factors-1(IGF-1)axis and Gs/PKA/CREB signaling pathways,as well as downstream osteogenic and osteoclastic differentiation factors.The effects of B.breve CCFM1078 were different with age and gender dependent.This finding suggests B.breve CCFM1078 may have potential applications in regulating bone metabolism in the growth period population.展开更多
Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to ...Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.展开更多
In the new era of‘‘Omics’’,the traditional techniques of protein expression in vivo can not come up with the exponential increase of genetic information.The cellfree protein synthesis system provides a new strateg...In the new era of‘‘Omics’’,the traditional techniques of protein expression in vivo can not come up with the exponential increase of genetic information.The cellfree protein synthesis system provides a new strategy of protein expression with advantages of rapid,convenient and high-throughput expression.The preparation of cell extracts,the optimization of substrate concentrations and the energy regeneration system are the key factors for the successful construction of cell-free protein expression system.In this work,the cell extract was prepared from RNase I-defective strain E.coli A19.The cell growth phase,the pressure for cell disruption and the storage condition of cell extracts were optimized.Meanwhile,the optimal substrate concentrations and the energy regeneration system were selected.Under the optimized conditions,the green fluorescent protein(GFP)reporter gene was expressed in the E.coli cell-free system with high expression level(Ca.154 mg/mL)which was 29 times higher than the expression level before optimization.展开更多
基金funded by the National Natural Science Foundation of China(31972052,32021005,31820103010)the Fundamental Research Funds for the Central Universities(JUSRP22006,JUSRP51501)the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu Province。
文摘Lipid metabolism disorders commonly occur during menopause.Estrogen deficiency has been shown to lead to excessive energy intake and abnormal lipid metabolism in ovariectomized rats,resulting in obesity.Probiotics exhibit anti-obesity properties,and their underlying mechanism has been widely reported.In this study,we demonstrated the metabolic benefits of Lactobacillus plantarum CCFM1180 in suppressing appetite,controlling body weight,correcting obesity-induced abnormalities,enhancing liver lipid metabolism,and protecting liver function in estrogen-deficient rats.The mechanisms associated with the anti-obesity and anti-dyslipidemia effects of CCFM1180 on estrogen-deficient rats were clarified.The results showed that CCFM1180 dramatically reduced food intake by activating the expression of estrogen receptor alpha(ERα)and increasing the level of leptin in abdominal adipose tissue.These changes,combined with the increased butyrate concentration and recovered bile acid structure,helped enhance lipid metabolism.Additionally,CCFM1180 treatment was found to be safer than exogenous estrogen supplementation.Thus,L.plantarum CCFM1180 could be considered a new therapeutic strategy for preventing and alleviating menopausal lipid abnormalities.
基金funded by the National Natural Science Foundation of China(32021005)the Fundamental Research Funds for the Central Universities(JUSRP622020,JUSRP51501)the Program of Collaborative Innovation Centre of Food Safety and Quality Control in Jiangsu Province.
文摘The decline in ovarian estrogen production is known to have detrimental health consequences and negatively affect the quality of life in menopausal women.Increasing estradiol levels is key to preventing disease in menopausal women.In this study,Lactobacillus gasseri CCFM1255 isolated from healthy infants was found to have a positive effect on estradiol production in ovariectomized rats.CYP19,the key enzyme catalysing the conversion of androgens into estrogens,was upregulated in adipose tissue upon CCFM1255 treatment.Untargeted metabolome analysis and targeted metabolite detection were used to identify the key metabolites altered by CCFM1255 treatment.CCFM1255 treatment significantly improved the serum concentration of glutamine(Gln).A significantly positive correlation was observed between serum Gln and estradiol concentrations.CCFM1255 treatment reshaped the structure of the gut microbiome,which was correlated with certain changes in serum metabolite concentrations.These results indicate that the provision of CCFM1255 as a dietary supplement may be an effective strategy to alleviate menopausal symptoms by increasing circulating estradiol.
基金National Key Research and Development Program of China(Grant No.2017YFC1502102)National Natural Science Foundation of China(Grant No.42075148)+1 种基金Graduate Research and Innovation Projects of Jiangsu Province(Grant No.KYCX20_0910)the High-Performance Computing Center of Nanjing University of Information Science and Technology(NUIST).
文摘Use of data assimilation to initialize hydrometeors plays a vital role in numerical weather prediction(NWP).To directly analyze hydrometeors in data assimilation systems from cloud-sensitive observations,hydrometeor control variables are necessary.Common data assimilation systems theoretically require that the probability density functions(PDFs)of analysis,background,and observation errors should satisfy the Gaussian unbiased assumptions.In this study,a Gaussian transform method is proposed to transform hydrometeors to more Gaussian variables,which is modified from the Softmax function and renamed as Quasi-Softmax transform.The Quasi-Softmax transform method then is compared to the original hydrometeor mixing ratios and their logarithmic transform and Softmax transform.The spatial distribution,the non-Gaussian nature of the background errors,and the characteristics of the background errors of hydrometeors in each method are studied.Compared to the logarithmic and Softmax transform,the Quasi-Softmax method keeps the vertical distribution of the original hydrometeor mixing ratios to the greatest extent.The results of the D′Agostino test show that the hydrometeors transformed by the Quasi-Softmax method are more Gaussian when compared to the other methods.The Gaussian transform has been added to the control variable transform to estimate the background error covariances.Results show that the characteristics of the hydrometeor background errors are reasonable for the Quasi-Softmax method.The transformed hydrometeors using the Quasi-Softmax transform meet the Gaussian unbiased assumptions of the data assimilation system,and are promising control variables for data assimilation systems.
基金supported by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘This study endeavors to investigate the effects of Bifidobacterium breve CCFM1078 on bone formation and resorption balance in growing BALB/c mice.Newborn BALB/c mice were assigned to the control group(administration saline)and the CCFM1078 group(administration B.breve CCFM1078,3×10^(9) CFU/day)in 3-,4-,and 5-week tests.All the groups have male and female distinctions.Our findings demonstrate that B.breve CCFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites(hexadecanamide,linoleoyl ethanolamide,and palmitoyl ethanolamide),the genes and proteins expression related to the growth hormone(GH)/insulin-like growth factors-1(IGF-1)axis and Gs/PKA/CREB signaling pathways,as well as downstream osteogenic and osteoclastic differentiation factors.The effects of B.breve CCFM1078 were different with age and gender dependent.This finding suggests B.breve CCFM1078 may have potential applications in regulating bone metabolism in the growth period population.
基金funded by the National Key R&D Program of China(2021YFD2100700)National Natural Science Foundation of China(32021005)+1 种基金111 project(BP0719028)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Bifidobacterium longum subsp.infantis is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune development.This study aimed to explore the effects of B.longum subsp.infantis supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing mice.Female and male mice were orally administered either saline or B.longum subsp.infantis CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and 5-week-age.RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway.However,this effect was not observed in 4-and 5-week-old mice.Furthermore,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old mice.In summary,the results of this study suggested that B.longum subsp.infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.
基金the National Natural Science Foundation of China(Grant Nos.20736008,20676115 and 20706023)The Ministry of Education(Grant No.20060335085),The People’s Republic of China.
文摘In the new era of‘‘Omics’’,the traditional techniques of protein expression in vivo can not come up with the exponential increase of genetic information.The cellfree protein synthesis system provides a new strategy of protein expression with advantages of rapid,convenient and high-throughput expression.The preparation of cell extracts,the optimization of substrate concentrations and the energy regeneration system are the key factors for the successful construction of cell-free protein expression system.In this work,the cell extract was prepared from RNase I-defective strain E.coli A19.The cell growth phase,the pressure for cell disruption and the storage condition of cell extracts were optimized.Meanwhile,the optimal substrate concentrations and the energy regeneration system were selected.Under the optimized conditions,the green fluorescent protein(GFP)reporter gene was expressed in the E.coli cell-free system with high expression level(Ca.154 mg/mL)which was 29 times higher than the expression level before optimization.
