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Overview of ferroptosis and pyroptosis in acute liver failure
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作者 Ya-Wen Sun Bo-Wen Zhao +1 位作者 hai-fang li Guang-Xiao Zhang 《World Journal of Gastroenterology》 SCIE CAS 2024年第34期3856-3861,共6页
In this editorial,we comment on the article by Zhou et al published in a recent issue.We specifically focus on the crucial roles of ferroptosis and pyroptosis in acute liver failure(ALF),a disease with high mortality ... In this editorial,we comment on the article by Zhou et al published in a recent issue.We specifically focus on the crucial roles of ferroptosis and pyroptosis in acute liver failure(ALF),a disease with high mortality rates.Ferroptosis is the result of increased intracellular reactive oxygen species due to iron accumulation,glutathione(GSH)depletion,and decreased GSH peroxidase 4 activity,while pyroptosis is a procedural cell death mediated by gasdermin D which initiates a sustained inflammatory process.In this review,we describe the characteristics of ferroptosis and pyroptosis,and discuss the involvement of the two cell death modes in the onset and development of ALF.Furthermore,we summarize several interfering methods from the perspective of ferroptosis and pyroptosis for the alleviation of ALF.These observations might provide new targets and a theoretical basis for the treatment of ALF,which are also crucial for improving the prognosis of patients with ALF. 展开更多
关键词 Acute liver failure Ferroptosis PYROPTOSIS Glutathione peroxidase 4 Gasdermin D
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Protein succinylation,hepatic metabolism,and liver diseases
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作者 Shuang liu Rui li +2 位作者 Ya-Wen Sun Hai lin hai-fang li 《World Journal of Hepatology》 2024年第3期344-352,共9页
Succinylation is a highly conserved post-translational modication that is processed via enzymatic and non-enzymatic mechanisms.Succinylation exhibits strong effects on protein stability,enzyme activity,and transcripti... Succinylation is a highly conserved post-translational modication that is processed via enzymatic and non-enzymatic mechanisms.Succinylation exhibits strong effects on protein stability,enzyme activity,and transcriptional regulation.Protein succinylation is extensively present in the liver,and increasing evidence has demonstrated that succinylation is closely related to hepatic metabolism.For instance,histone acetyltransferase 1 promotes liver glycolysis,and the sirtuin 5-induced desuccinylation is involved in the regulation of the hepatic urea cycle and lipid metabolism.Therefore,the effects of succinylation on hepatic glucose,amino acid,and lipid metabolism under the action of various enzymes will be discussed in this work.In addition,how succinylases regulate the progression of different liver diseases will be reviewed,including the desuccinylation activity of sirtuin 7,which is closely associated with fatty liver disease and hepatitis,and the actions of lysine acetyltransferase 2A and histone acetyltransferase 1 that act as succinyltransferases to regulate the succinylation of target genes that influence the development of hepatocellular carcinoma.In view of the diversity and significance of protein succinylation,targeting the succinylation pathway may serve as an attractive direction for the treatment of liver diseases. 展开更多
关键词 Protein succinylation Hepatic metabolism Fatty liver HEPATITIS Hepatocellular carcinoma
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Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography 被引量:4
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作者 Lei-Wen Xiang Jing li +1 位作者 Jin-Ming lin hai-fang li 《Journal of Pharmaceutical Analysis》 SCIE CAS 2014年第2期153-158,共6页
Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in... Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26 (5:95). Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957-0.9993. The proposed analytical method has satisfactory repeatability (the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49-97.90%, 95.38-96.45%, 112.46-115.78%and 90.82-97.13%with standard deviation of o5%, respectively) and the limits of detection (LODs, S/N Z 3) for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L, respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis' biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis. 展开更多
关键词 Gouty arthritis CREATININE Uric acid HYPOXANTHINE XANTHINE High-performance liquid chromatography
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Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from Cordyceps militaris
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作者 Jian Chen hai-fang li +2 位作者 Guozhu Zhao Jin-Ming lin Xiangwei He 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2021年第4期499-504,共6页
Cordycepin,which has great immunomodulatory activities such as anticancer,antifungal,antivirus,antileukemia and lipid-lowering ones,is the secondary metabolite of Cordyceps militaris(C.militaris).Liquid submerged ferm... Cordycepin,which has great immunomodulatory activities such as anticancer,antifungal,antivirus,antileukemia and lipid-lowering ones,is the secondary metabolite of Cordyceps militaris(C.militaris).Liquid submerged fermentation is the common cultivation process to produce cordycepin.To optimize the fermentation process and improve production,monitoring the cordycepin secretion in the fermentation is essential.The measurement based on chromatography-mass spectrometry methods is generally involved in the complex sample pretreatments and time-consuming separation,so more rapid and convenient methods are required.Matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS)is more attractive for faster and direct detection.Therefore,MALDI-MS detection combined with isotope-labeled internal standard was applied to the measurement of cordycepin content in the fermentation broth and mycelium.This method made accurate quantification of cordycepin in the range of 5-400μg/mL with a relative standard deviation of 5.6%.The recovery rates of fermentation samples after the 1,13,and 25 days were 90.15%,94.27%,and 95.06%,respectively.The contents of cordycepin in the mycelium and fermentation broth were 136 mg/g and 148.39 mg/mL on the 20 th culture day,respectively.The cordycepin secretion curve of the liquid fermentation of C.militaris was real-time traced over 25 days. 展开更多
关键词 CORDYCEPIN MALDI-MS Isotope internal standard Quantitative analysis Liquid fermentation
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Brain functional network connectivity based on a visual task: visual information processing-related brain regions are significantly activated in the task state 被引量:2
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作者 Yan-li Yang Hong-xia Deng +2 位作者 Gui-yang Xing Xiao-luan Xia hai-fang li 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第2期298-307,共10页
It is not clear whether the method used in functional brain-network related research can be applied to explore the feature binding mechanism of visual perception. In this study, we inves-tigated feature binding of col... It is not clear whether the method used in functional brain-network related research can be applied to explore the feature binding mechanism of visual perception. In this study, we inves-tigated feature binding of color and shape in visual perception. Functional magnetic resonance imaging data were collected from 38 healthy volunteers at rest and while performing a visual perception task to construct brain networks active during resting and task states. Results showed that brain regions involved in visual information processing were obviously activated during the task. The components were partitioned using a greedy algorithm, indicating the visual network existed during the resting state.Z-values in the vision-related brain regions were calculated, conifrming the dynamic balance of the brain network. Connectivity between brain regions was determined, and the result showed that occipital and lingual gyri were stable brain regions in the visual system network, the parietal lobe played a very important role in the binding process of color features and shape features, and the fusiform and inferior temporal gyri were crucial for processing color and shape information. Experimental ifndings indicate that understanding visual feature binding and cognitive processes will help establish computational models of vision, improve image recognition technology, and provide a new theoretical mechanism for feature binding in visual perception. 展开更多
关键词 nerve regeneration functional magnetic resonance imaging resting state task state brain network module division feature binding Fisher’s Z transform CONNECTIVITY visual stimuli NSFC grants neural regeneration
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H2 Dissociation by Au1-Doped Closed-Shell Titanium Oxide Cluster Anions
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作者 li-xue Jiang Xiao-na li +2 位作者 Zi-yu li hai-fang li Sheng-gui He 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2018年第4期457-462,614,共7页
Dissociation of molecular hydrogen (H2) is extensively studied to understand the mechanism of hydrogenation reactions. In this study, H2 dissociation by Aul-doped closed-shell titanium oxide cluster anions AuTi3O7 a... Dissociation of molecular hydrogen (H2) is extensively studied to understand the mechanism of hydrogenation reactions. In this study, H2 dissociation by Aul-doped closed-shell titanium oxide cluster anions AuTi3O7 and AuTi3O8 has been identified by mass spectrometry and quantum chemistry calculations. The clusters were generated by laser ablation and mass- selected to react with H2 in art ion trap reactor. In the reaction of AuTi3O8 with H2, the ion pair Au+-O22 rather than Au+-O2 is the active site to promote H2 dissociation. This finding is in contrast with the previous result that the lattice oxygen is usually the reactive oxygen species in H2 dissociation. The higher reactivity of the peroxide species is further supported by frontier molecular orbital analysis. This study provides new insights into gold catalysis involving H2 activation and dissociation. 展开更多
关键词 Gold H2 dissociation Closed-shell anions Mass spectrometry Density func tional theory calculations
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Site-Specific Photochemical Reaction for Improved C=C Location Analysis of Unsaturated Lipids by Ultraviolet Photodissociation 被引量:2
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作者 hai-fang li Jing Zhao +3 位作者 Wenbo Cao Wenpeng Zhang Yu Xia Zheng Ouyang 《Research》 EI CAS CSCD 2022年第2期283-294,共12页
Unraveling the complexity of the lipidome requires the development of novel approaches to facilitate structural identification and characterization of lipid species with isomer-level discrimination.Ultraviolet photodi... Unraveling the complexity of the lipidome requires the development of novel approaches to facilitate structural identification and characterization of lipid species with isomer-level discrimination.Ultraviolet photodissociation tandem mass spectrometry(UVPD MS/MS)is a promising tool for structure determination of lipids.The sensitivity of UVPD for lipid analysis however is.limited mainly due to weak absorption of UV photons bya C=C.Herein,a C=C site-specific derivatization,the Paterno-Bichi.(PB)reaction,was used to incorporate a chromophore to the C=C moiety in fatty acyls,leading to significantly improved UVPD eficiency and sensitivity for pinpointing C=C locations.The wavelength-dependent photodissociation of the PB products demonstrated 4-CF;-benzophenone as the best reagent for UVPD in terms of the efficiency of generating C=C diagnostic fragments and simplicity for C=C location assignments.We demonstrated the efectiveness of this approach for the shotgun profiling of C=C location isomers in diferent lipid classes from complex lipid extraclis,highlighting its potential to advancing the identification of the C=C bond locations in unsaturated lipids. 展开更多
关键词 DISSOCIATION ISOMERS SIMPLICITY
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Determination of polybrominated diphenyl ethers in river water by combination of liquid–liquid extraction and gas chromatography–mass spectrometry
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作者 Weifei Zhang Xuexia lin +2 位作者 hai-fang li Zhihua Wang Jin-Ming lin 《Chinese Chemical Letters》 SCIE CAS CSCD 2014年第9期1225-1229,共5页
In this work, a reliable and sensitive method for detecting polybrominated diphenyl ethers (PBDEs) has been developed by the combination of liquid-liquid extraction and gas chromatography-mass spectrometry. PBDEs we... In this work, a reliable and sensitive method for detecting polybrominated diphenyl ethers (PBDEs) has been developed by the combination of liquid-liquid extraction and gas chromatography-mass spectrometry. PBDEs were extracted from a large volume of water by liquid-liquid extraction and purified by silica gel chromatography. In order to reduce the deviation, dibromobiphenyl was exploited as the internal standard to minimize differences among the injections. The quantification was performed using an external standard. Good linear correlation coefficients (〉0.991) and a wide linearity range ( 1,0- 500.0 ng/L) indicated the steadiness of the proposed method. Moreover, the satisfactory recovery (〉75%) suggested that successful determination of PBDEs in river water had been achieved. Furthermore, the deduction behavior of PBDEs in river water could be inferred according to the results. 展开更多
关键词 Polybrominated diphenyl ethers Dibromobiphenyl GC-MS Liquid-liquid extraction
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Determination of ammonium on an integrated microchip with LED-induced fluorescence detection 被引量:3
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作者 Shuhua Xue Katsumi Uchiyama hai-fang li 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2012年第3期564-570,共7页
A simply fabricated microfluidic device integrated with a fluorescence detection system has been developed for on-line determination of ammonium in aqueous samples. A 365-nm light-emitting diode (LED) as an excitati... A simply fabricated microfluidic device integrated with a fluorescence detection system has been developed for on-line determination of ammonium in aqueous samples. A 365-nm light-emitting diode (LED) as an excitation source and a minor band pass filter were mounted into a polydimethylsiloxane (PDMS)-based microchip for the purpose of miniaturization of the entire analytical system. The ammonium sample reacted with o-phthaldialdehyde (OPA) on-chip with sodium sulfite as reducing reagent to produce a fluorescent isoindole derivative, which can emit fluorescence signal at about 425 nm when excited at 365 nm. Effects of pH, flow rate of solutions, concentrations of OPA-reagent, phosphate and sulfite salt were investigated. The calibration curve of ammonium in the range of 0.018- 1.8 μg/mL showed a good linear relationship with R2 = 0.9985, and the detection limit was (S /N = 3) 3.6 × 10 4 μg/mL. The relative standard deviation was 2.8% (n = 11) by calculating at 0.18 μg/mL ammonium for repeated detection. The system was applied to determine the ammonium concentration in rain and river waters, even extent to other analytes fluorescence detection by the presented device. 展开更多
关键词 microfluidic chip light emitting diode O-PHTHALDIALDEHYDE FLUORESCENCE AMMONIUM
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Chip-based SALDI-MS for rapid determination of intracellular ratios of glutathione to glutathione disulfide 被引量:1
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作者 Min li Sifeng Mao +2 位作者 Shiqi Wang hai-fang li Jin-Ming lin 《Science China Chemistry》 SCIE EI CAS CSCD 2019年第1期142-150,共9页
Alterations in the ratio of glutathione(GSH) to glutathione disulfide(GSSG) reveal the cell living state and are associated with a variety of diseases. In this study, an Au NPs grafted nanoporous silicon chip was used... Alterations in the ratio of glutathione(GSH) to glutathione disulfide(GSSG) reveal the cell living state and are associated with a variety of diseases. In this study, an Au NPs grafted nanoporous silicon chip was used for surface assisted laser desorption ionization-mass spectrometry(SALDI-MS) detection of GSH. Due to the bond interaction between thiol of GSH and Au NPs modified on the chip surfaces, GSH could be captured from the complex cellular lysate. Meanwhile, the composite nanostructures of Au NPs grafted porous silicon surface presented good desorption/ionization efficiency for GSH detection. The GSH levels in different tumor cells were successfully detected. Chip-based SALDI-MS was optimized for quantification of intracellular GSH/GSSG ratio changing under drug stimulation in liver tumor cells, GSSG was reduced to GSH by reductant of tris(2-carboxyethyl)phosphine(TCEP) and isotope-labeling GSH was as an internal standard. It was found that the increasing concentration of drug irinotecan and hypoxia culture condition caused the rapid consumption of GSH and a decrease of GSH/GSSG ratio in liver tumor cells. The developed SALDI-MS method provided a convenient way to accurately measure and rapidly monitor cellular GSH value and the ratios of GSH/GSSG. 展开更多
关键词 GLUTATHIONE GLUTATHIONE DISULFIDE GOLD nanoparticles SALDI-MS
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Enhanced chemiluminescence from reactions between CdTe/CdS/ZnS quantum dots and periodate 被引量:2
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作者 Yu li Yong-Zan Zheng +3 位作者 Ding-Kun Zhang hai-fang li Yuan Ma Jin-Ming lin 《Chinese Chemical Letters》 SCIE CAS CSCD 2017年第2期184-188,共5页
A novel chemiluminescence(CL) performance of CdTe/CdS/ZnS quantum dots(QDs) with periodate(KIO_4) was studied.Effects of concentration and pH on the CL system were investigated.Electron spin resonance(ESR) and... A novel chemiluminescence(CL) performance of CdTe/CdS/ZnS quantum dots(QDs) with periodate(KIO_4) was studied.Effects of concentration and pH on the CL system were investigated.Electron spin resonance(ESR) and the effects of radical scavenger analysis were employed for identification of intermediate species.The CL spectra for this system showed only one maximum emission peak centered around 620 nm,which was similar with photoluminescence(PL) spectra of CdTe/CdS/ZnS QDs.The CL of CdTe/CdS/ZnS QDs was induced by direct chemical oxidation and the possible mechanism could be explained by radiative recombination of injected holes and electrons.This investigation not only provided new sight into the optical characteristics of CdTe/CdS/ZnS QDs,but also broadened their potential optical utilizations. 展开更多
关键词 Chemiluminescence CdTe/CdS/ZnS quantum dots Periodate Electron injection Hole injection
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