本研究以橄榄果为原料发酵生产橄榄果酒,通过单因素试验探索了酵母接种量、初始糖度、发酵时间、发酵温度、初始p H值对橄榄果酒感官品质的影响,用正交试验和验证试验,确定了橄榄果酒最佳的发酵工艺条件,结果表明:在初始p H 4.0,酵母接...本研究以橄榄果为原料发酵生产橄榄果酒,通过单因素试验探索了酵母接种量、初始糖度、发酵时间、发酵温度、初始p H值对橄榄果酒感官品质的影响,用正交试验和验证试验,确定了橄榄果酒最佳的发酵工艺条件,结果表明:在初始p H 4.0,酵母接种量7%,初始糖度22°Bx,28℃条件下发酵9 d,获得的橄榄果酒酒精度达(12.20±0.45)%vol,含糖量为5.26 g/L,酸度为5.46g/L。最佳工艺条件下,成品酒酸涩适中,果香浓郁,酒液微黄透亮,后味清爽,理化指标达到相关标准。通过固相微萃取和气相色谱质谱联用法检测分析发现橄榄果酒中含有74种风味物质,其中酯类26种、醇类13种、烷类4种、烯类12种、酚类1种、醚类1种,其他17种,其中,乙酸丁酯、苯乙醇、异戊醇、癸酸乙酯、月桂酸乙酯、乙酸苯乙酯、α-律草烯、α-蒎烯、β-榄香烯等萜烯类物质对滋味有显著贡献作用,使成品酒风格突出,赋予了橄榄果酒独特的口感。展开更多
This study investigates the developmental changes of the lipoprotein lipase （LPL） mRNA level in sheep muscle and its effect on intramuscular fat （IMF） accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 da...This study investigates the developmental changes of the lipoprotein lipase （LPL） mRNA level in sheep muscle and its effect on intramuscular fat （IMF） accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 days old were selected. Six animals of each breed were slaughtered at 2, 30, 60, 90, and 120 days （only the Xinjiang Merino sheep at 120-day old were available） to collect samples from longissimus dorsi muscle for the purpose of determining the IMF content and extracting total RNA that was used to investigate the developmental changes of the LPL mRNA expression by real-time PCR. The results showed that in male Kazak sheep, the IMF content increased with the progress of development and there were significant differences （P〈0.05） between the age groups. However, there was no difference （P〉0.05） between age groups in Xinjiang Merino sheep. Furthermore, the IMF content of the male Kazak sheep was significantly higher （P 〈 0.01） than that of the Xinjiang Merino sheep aged from 30 to 90 days. The highest LPL mRNA expression appeared at day 2 and it was significantly higher than that of all other age groups （P 〈 0.01）, while animals at 60-day old had the lowest LPL mRNA expression in the male Kazak sheep. In Xinjiang Merino sheep, the highest one occurred at 30-day old （P〈0.01）, followed by a continuous decrease to the lowest level at 90-day old, and then it started to increase slightly. At 2 to 60-day old, the LPL mRNA expression was negatively correlated to the IMF content （r=-0.625, P 〈 0.05） in male Kazak sheep, but no such relationship was detected in the male Xinjiang Merino sheep.展开更多
文摘This study investigates the developmental changes of the lipoprotein lipase （LPL） mRNA level in sheep muscle and its effect on intramuscular fat （IMF） accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 days old were selected. Six animals of each breed were slaughtered at 2, 30, 60, 90, and 120 days （only the Xinjiang Merino sheep at 120-day old were available） to collect samples from longissimus dorsi muscle for the purpose of determining the IMF content and extracting total RNA that was used to investigate the developmental changes of the LPL mRNA expression by real-time PCR. The results showed that in male Kazak sheep, the IMF content increased with the progress of development and there were significant differences （P〈0.05） between the age groups. However, there was no difference （P〉0.05） between age groups in Xinjiang Merino sheep. Furthermore, the IMF content of the male Kazak sheep was significantly higher （P 〈 0.01） than that of the Xinjiang Merino sheep aged from 30 to 90 days. The highest LPL mRNA expression appeared at day 2 and it was significantly higher than that of all other age groups （P 〈 0.01）, while animals at 60-day old had the lowest LPL mRNA expression in the male Kazak sheep. In Xinjiang Merino sheep, the highest one occurred at 30-day old （P〈0.01）, followed by a continuous decrease to the lowest level at 90-day old, and then it started to increase slightly. At 2 to 60-day old, the LPL mRNA expression was negatively correlated to the IMF content （r=-0.625, P 〈 0.05） in male Kazak sheep, but no such relationship was detected in the male Xinjiang Merino sheep.
