目的分析昼夜节律与骨代谢领域研究现况、热点及发展趋势,以期为日后研究提供参考。方法以Web of Science核心数据库2000年1月1日至2022年11月30日收录文献为基础,采用VOSviewer 1.6.16和CiteSpace 6.1.R4软件对文献发表年度、国家/地...目的分析昼夜节律与骨代谢领域研究现况、热点及发展趋势,以期为日后研究提供参考。方法以Web of Science核心数据库2000年1月1日至2022年11月30日收录文献为基础,采用VOSviewer 1.6.16和CiteSpace 6.1.R4软件对文献发表年度、国家/地区、机构、作者、期刊、关键词、引用情况等进行数据可视化分析。结果共检索295篇文献,发文量总体趋于平缓稳定,在地域上广泛分布于美国、英国、亚洲、欧洲等多个国家和地区,其中美国、英国在论文数量和深度上颇为突出,研究机构以大学及其附属医疗机构为主,2010年后各机构和作者间联系较为紧密。研究内容上聚焦于昼夜节律与骨吸收、骨转化等骨代谢间机制的探索,包含骨代谢标志物、基因表达等维度。研究前沿包括Rev-erbα、骨折风险等。结论昼夜节律和骨代谢相关文献的刊文量仍相对较少,应进一步发挥高质量期刊的引领作用,以期为骨代谢异常防控提供新的思路和治疗方案。未来基于基因表达层面的影响机制以及如何在昼夜节律视角下精准降低骨折风险仍是研究的重点方向。展开更多
Objective To study the antidepressant-like effect and action mechanism of geniposide and eleutheroside B combination treatment on the lipopolysaccharide(LPS)-induced depression mice model.Methods Depression mice model...Objective To study the antidepressant-like effect and action mechanism of geniposide and eleutheroside B combination treatment on the lipopolysaccharide(LPS)-induced depression mice model.Methods Depression mice model was established by lipopolysaccharide(LPS)injection.Totally 48 mice were randomly divided into 6 groups(8 rats per group)according to a random number table,including normal,model,fluoxetine(20 mg/kg),geniposide(100 mg/kg)+eleutheroside B(100 mg/kg),geniposide+eleutheroside B+WAY 100635(0.03 mg/kg),geniposide+eleutheroside B+N-methyl-D-aspartic acid receptor(NMDA,75 mg/kg)groups,respectively.After continuous administration for 10 days,autonomic activity tests after 30 min of administration were performed on the 10th day.On the 11th day,except for the normal group,the mice in the other groups were intraperitoneally injected with LPS(1 mg/kg),and the behavioral tests were performed 4 h later.Enzyme linked immunosorbent assay was used to detect tumor necrosis factor alpha(TNF-α)and interleukin-1β(IL-1β)levels in mice serum.The mRNA expression of indoleamine 2,3-dioxygenase(IDO)and nuclear transcription factor(NF-κB)were detected by real-time quantitative polymerase chain reaction.Western-blot analysis was used to detect IDO and NF-κB protein expressions in hippocampus tissue.Results Compared with the normal group,a single administration of LPS increased the immobility time in the forced swimming test(FST)and tail suspension test(TST,P<0.01),without affecting autonomous activity.Compared with the model group,fluoxetine and geniposide+eleutheroside B administration significantly improved the immobility time of depressed mice in the FST and TST,decreased serum IL-1βcontent,inhibited the expression levels of NF-κB gene and protein in hippocampus tissues(P<0.05 or P<0.01).Compared with the model group,geniposide+eleutheroside B treatment significantly reduced serum TNF-αcontent and inhibited IDO mRNA and protein expressions in hippocampus(P<0.05 or P<0.01).In addition,NMDA partly prevented the inhibition of IDO mRNA expression by geniposide+eleutheroside B;NMDA and WAY-100635 also partly prevented the reduction of IL-1ßcontent induced by geniposide+eleutheroside B treatment(P<0.05 or P<0.01).Conclusions The combination of geniposide and eleutheroside B showed a certain antidepression-like effect.Its main mechanism of action may be contributed to inhibiting the activation of NF-κB,decreasing the proinflammatory cytokines such as TNF-α,IL-1β,and inhibiting in the neuroinflammatory reaction.Additionally,it also affects tryptophan metabolism,reduces the expression of a key enzyme of tryptophan metabolism,IDO.And this antidepressant-like effect may be mediated by 5-hydroxytryptamine and glutamate systems.展开更多
文摘目的分析昼夜节律与骨代谢领域研究现况、热点及发展趋势,以期为日后研究提供参考。方法以Web of Science核心数据库2000年1月1日至2022年11月30日收录文献为基础,采用VOSviewer 1.6.16和CiteSpace 6.1.R4软件对文献发表年度、国家/地区、机构、作者、期刊、关键词、引用情况等进行数据可视化分析。结果共检索295篇文献,发文量总体趋于平缓稳定,在地域上广泛分布于美国、英国、亚洲、欧洲等多个国家和地区,其中美国、英国在论文数量和深度上颇为突出,研究机构以大学及其附属医疗机构为主,2010年后各机构和作者间联系较为紧密。研究内容上聚焦于昼夜节律与骨吸收、骨转化等骨代谢间机制的探索,包含骨代谢标志物、基因表达等维度。研究前沿包括Rev-erbα、骨折风险等。结论昼夜节律和骨代谢相关文献的刊文量仍相对较少,应进一步发挥高质量期刊的引领作用,以期为骨代谢异常防控提供新的思路和治疗方案。未来基于基因表达层面的影响机制以及如何在昼夜节律视角下精准降低骨折风险仍是研究的重点方向。
基金Supported by the Shandong Key Research and Development Plan(No.2017GSF19112)。
文摘Objective To study the antidepressant-like effect and action mechanism of geniposide and eleutheroside B combination treatment on the lipopolysaccharide(LPS)-induced depression mice model.Methods Depression mice model was established by lipopolysaccharide(LPS)injection.Totally 48 mice were randomly divided into 6 groups(8 rats per group)according to a random number table,including normal,model,fluoxetine(20 mg/kg),geniposide(100 mg/kg)+eleutheroside B(100 mg/kg),geniposide+eleutheroside B+WAY 100635(0.03 mg/kg),geniposide+eleutheroside B+N-methyl-D-aspartic acid receptor(NMDA,75 mg/kg)groups,respectively.After continuous administration for 10 days,autonomic activity tests after 30 min of administration were performed on the 10th day.On the 11th day,except for the normal group,the mice in the other groups were intraperitoneally injected with LPS(1 mg/kg),and the behavioral tests were performed 4 h later.Enzyme linked immunosorbent assay was used to detect tumor necrosis factor alpha(TNF-α)and interleukin-1β(IL-1β)levels in mice serum.The mRNA expression of indoleamine 2,3-dioxygenase(IDO)and nuclear transcription factor(NF-κB)were detected by real-time quantitative polymerase chain reaction.Western-blot analysis was used to detect IDO and NF-κB protein expressions in hippocampus tissue.Results Compared with the normal group,a single administration of LPS increased the immobility time in the forced swimming test(FST)and tail suspension test(TST,P<0.01),without affecting autonomous activity.Compared with the model group,fluoxetine and geniposide+eleutheroside B administration significantly improved the immobility time of depressed mice in the FST and TST,decreased serum IL-1βcontent,inhibited the expression levels of NF-κB gene and protein in hippocampus tissues(P<0.05 or P<0.01).Compared with the model group,geniposide+eleutheroside B treatment significantly reduced serum TNF-αcontent and inhibited IDO mRNA and protein expressions in hippocampus(P<0.05 or P<0.01).In addition,NMDA partly prevented the inhibition of IDO mRNA expression by geniposide+eleutheroside B;NMDA and WAY-100635 also partly prevented the reduction of IL-1ßcontent induced by geniposide+eleutheroside B treatment(P<0.05 or P<0.01).Conclusions The combination of geniposide and eleutheroside B showed a certain antidepression-like effect.Its main mechanism of action may be contributed to inhibiting the activation of NF-κB,decreasing the proinflammatory cytokines such as TNF-α,IL-1β,and inhibiting in the neuroinflammatory reaction.Additionally,it also affects tryptophan metabolism,reduces the expression of a key enzyme of tryptophan metabolism,IDO.And this antidepressant-like effect may be mediated by 5-hydroxytryptamine and glutamate systems.
