In recent years,a number of wireless indoor positioning(WIP),such as Bluetooth,Wi-Fi,and Ultra-Wideband(UWB)technologies,are emerging.However,the indoor environment is complex and changeable.Walls,pillars,and even ped...In recent years,a number of wireless indoor positioning(WIP),such as Bluetooth,Wi-Fi,and Ultra-Wideband(UWB)technologies,are emerging.However,the indoor environment is complex and changeable.Walls,pillars,and even pedestrians may block wireless signals and produce non-line-of-sight(NLOS)deviations,resulting in decreased positioning accuracy and the inability to provide people with real-time continuous indoor positioning.This work proposed a strong tracking particle filter based on the chi-square test(SPFC)for indoor positioning.SPFC can fuse indoor wireless signals and the information of the inertial sensing unit(IMU)in the smartphone and detect the NLOS deviation through the chi-square test to avoid the influence of the NLOS deviation on the final positioning result.Simulation experiment results show that the proposed SPFC can reduce the positioning error by 15.1%and 12.3% compared with existing fusion positioning systems in the LOS and NLOS environment.展开更多
Alternative polyadenylation(APA)contributes to transcriptome complexity and gene expression regulation and has been implicated in various cellular processes and diseases.Singlecell RNA sequencing(scRNA-seq)has enabled...Alternative polyadenylation(APA)contributes to transcriptome complexity and gene expression regulation and has been implicated in various cellular processes and diseases.Singlecell RNA sequencing(scRNA-seq)has enabled the profiling of APA at the single-cell level;however,the spatial information of cells is not preserved in scRNA-seq.Alternatively,spatial transcriptomics(ST)technologies provide opportunities to decipher the spatial context of the transcriptomic landscape.Pioneering studies have revealed potential spatially variable genes and/or splice isoforms;however,the pattern of APA usage in spatial contexts remains unappreciated.In this study,we developed a toolkit called stAPAminer for mining spatial patterns of APA from spatially barcoded ST data.APA sites were identified and quantified from the ST data.In particular,an imputation model based on the k-nearest neighbors algorithm was designed to recover APA signals,and then APA genes with spatial patterns of APA usage variation were identified.By analyzing wellestablished ST data of the mouse olfactory bulb(MOB),we presented a detailed view of spatial APA usage across morphological layers of the MOB.We compiled a comprehensive list of genes with spatial APA dynamics and obtained several major spatial expression patterns that represent spatial APA dynamics in different morphological layers.By extending this analysis to two additional replicates of the MOB ST data,we observed that the spatial APA patterns of several genes were reproducible among replicates.stAPAminer employs the power of ST to explore the transcriptional atlas of spatial APA patterns with spatial resolution.This toolkit is available at https://github.com/BMILAB/stAPAminer and https://ngdc.cncb.ac.cn/biocode/tools/BT007320.展开更多
基金funded by the project“Design of System Integration Construction Scheme Based on Functions of Each Module” (No.XDHT2020169A)the project“Development of Indoor Inspection Robot System for Substation” (No.XDHT2019501A).
文摘In recent years,a number of wireless indoor positioning(WIP),such as Bluetooth,Wi-Fi,and Ultra-Wideband(UWB)technologies,are emerging.However,the indoor environment is complex and changeable.Walls,pillars,and even pedestrians may block wireless signals and produce non-line-of-sight(NLOS)deviations,resulting in decreased positioning accuracy and the inability to provide people with real-time continuous indoor positioning.This work proposed a strong tracking particle filter based on the chi-square test(SPFC)for indoor positioning.SPFC can fuse indoor wireless signals and the information of the inertial sensing unit(IMU)in the smartphone and detect the NLOS deviation through the chi-square test to avoid the influence of the NLOS deviation on the final positioning result.Simulation experiment results show that the proposed SPFC can reduce the positioning error by 15.1%and 12.3% compared with existing fusion positioning systems in the LOS and NLOS environment.
基金supported by the National Natural Science Foundation of China(Grant Nos.T2222007 to Xiaohui Wu,61573296 to Guoli Ji,and 81901287 to Shuting Xia)the Suzhou City People’s Livelihood Science and Technology Project,China(Grant No.SYS2020086 to Shuting Xia).
文摘Alternative polyadenylation(APA)contributes to transcriptome complexity and gene expression regulation and has been implicated in various cellular processes and diseases.Singlecell RNA sequencing(scRNA-seq)has enabled the profiling of APA at the single-cell level;however,the spatial information of cells is not preserved in scRNA-seq.Alternatively,spatial transcriptomics(ST)technologies provide opportunities to decipher the spatial context of the transcriptomic landscape.Pioneering studies have revealed potential spatially variable genes and/or splice isoforms;however,the pattern of APA usage in spatial contexts remains unappreciated.In this study,we developed a toolkit called stAPAminer for mining spatial patterns of APA from spatially barcoded ST data.APA sites were identified and quantified from the ST data.In particular,an imputation model based on the k-nearest neighbors algorithm was designed to recover APA signals,and then APA genes with spatial patterns of APA usage variation were identified.By analyzing wellestablished ST data of the mouse olfactory bulb(MOB),we presented a detailed view of spatial APA usage across morphological layers of the MOB.We compiled a comprehensive list of genes with spatial APA dynamics and obtained several major spatial expression patterns that represent spatial APA dynamics in different morphological layers.By extending this analysis to two additional replicates of the MOB ST data,we observed that the spatial APA patterns of several genes were reproducible among replicates.stAPAminer employs the power of ST to explore the transcriptional atlas of spatial APA patterns with spatial resolution.This toolkit is available at https://github.com/BMILAB/stAPAminer and https://ngdc.cncb.ac.cn/biocode/tools/BT007320.
